Compound probiotic bacteria liquid, preparation method thereof, and compound probiotic fermented feed made therefrom

A technology of compound probiotics and fermented feed, applied in the field of compound probiotics fermented feed, can solve problems such as single function, and achieve the effects of simple preparation process, promotion of absorption, and low cost of raw materials

Active Publication Date: 2022-01-14
MINNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing fermented feeds currently on the market have a single function and cannot be fermented feeds that can improve the immune function of animals and promote the growth and development of animals.

Method used

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  • Compound probiotic bacteria liquid, preparation method thereof, and compound probiotic fermented feed made therefrom

Examples

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Effect test

preparation example Construction

[0032] The preparation method of described compound probiotic bacterial liquid, it comprises the following processing steps:

[0033] (1) Preparation of nutrient solution and fermentation broth; (2) Activation of various strains; (3) Preparation of Lactobacillus johnsonii seed suspension, Lactobacillus bulgaricus seed suspension, Bifidobacterium seed suspension, Pichia burtonii seed suspension Suspension; (4) preparation of Lactobacillus johnsonii inoculum, Lactobacillus bulgaricus inoculum, bifidobacterium inoculum, Pichia burtonii inoculum; (5) preparation of bacterial liquid;

[0034] Wherein, the concrete preparation method of nutrient solution and fermented liquid in the step (1) is:

[0035] a. Collect the industrial waste water produced during the canning process of Agaricus bisporus;

[0036] b. Centrifuge the collected industrial wastewater produced during the canning process of Agaricus bisporus, and take the supernatant; during centrifugation, the speed of the cent...

Embodiment 1

[0069] A compound probiotic bacterial liquid, in terms of parts by weight, it comprises 8 parts of nutrient liquid, 15 parts of Lactobacillus johnsonii bacterial agent, 30 parts of Lactobacillus bulgaricus bacterial agent, 15 parts of Bifidobacterium bacterial agent, Burton Bi Red yeast inoculant 5 parts.

[0070] The preparation method of described compound probiotic bacterial liquid, it comprises the following processing steps:

[0071] (1) Preparation of nutrient solution and fermentation broth; (2) Activation of various strains; (3) Preparation of Lactobacillus johnsonii seed suspension, Lactobacillus bulgaricus seed suspension, Bifidobacterium seed suspension, Pichia burtonii seed suspension Suspension; (4) preparation of Lactobacillus johnsonii inoculum, Lactobacillus bulgaricus inoculum, bifidobacterium inoculum, Pichia burtonii inoculum; (5) preparation of bacterial liquid;

[0072] Wherein, the concrete preparation method of nutrient solution and fermented liquid in ...

Embodiment 2

[0100] A kind of composite probiotic bacterial liquid, by weight ratio, it comprises 9 parts of nutrient liquids, 10 parts of Lactobacillus johnsonii bacterial agents, 26 parts of Lactobacillus bulgaricus bacterial agents, 18 parts of Bifidobacterium bacterial agents, Burton Bi Red yeast inoculant 6 parts.

[0101] The preparation method of described compound probiotic bacterial liquid, it comprises the following processing steps:

[0102] (1) Preparation of nutrient solution and fermentation broth; (2) Activation of various strains; (3) Preparation of Lactobacillus johnsonii seed suspension, Lactobacillus bulgaricus seed suspension, Bifidobacterium seed suspension, Pichia burtonii seed suspension Suspension; (4) preparation of Lactobacillus johnsonii inoculum, Lactobacillus bulgaricus inoculum, bifidobacterium inoculum, Pichia burtonii inoculum; (5) preparation of bacterial liquid;

[0103] Wherein, the concrete preparation method of nutrient solution and fermented liquid in...

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Abstract

The present invention relates to a compound probiotic bacteria liquid and its preparation method and the compound probiotic fermented feed made from it. The compound probiotic bacteria liquid, in terms of parts by weight, comprises 8-10 parts of nutrient solution, about 8-15 parts of Lactobacillus inoculum, 25-30 parts of Lactobacillus bulgaricus, 15-20 parts of Bifidobacterium inoculum, 5-8 parts of Pichia Burtonii inoculum; , Lactobacillus bulgaricus, Bifidobacterium, and Pichia burtonii were prepared from fermentation broth and corresponding bacteria; Precipitation, the solution obtained by dissolving the precipitation in water; the fermented liquid is the solution obtained after the processing wastewater of Agaricus bisporus canning is concentrated, ethanol precipitation, filtration to remove the precipitate, the filtrate is collected, and the filtrate is concentrated; the canned Agaricus bisporus in the present invention The processing waste water can be fully utilized, saving the treatment cost of the processing waste water in canned Agaricus bisporus.

Description

technical field [0001] The invention relates to a compound probiotic bacterial liquid, a preparation method thereof and a compound probiotic fermented feed prepared therefrom. Background technique [0002] Agaricus bisporus (Lange) Sing is scientifically known as Agaricus bisporus (Lange) Sing, and the Chinese aliases are mushrooms and mushrooms. It is currently the most widely cultivated edible fungus with the highest output and largest consumption in the world, accounting for about 25% of the world's total output of edible fungi about. Due to the short storage period of fresh Agaricus bisporus, its international trade is mainly based on canned processed products, and the fresh mushrooms must be pre-cooked in time to prevent the mushrooms from opening. The weight of cooked mushrooms is 35%-40% lower than that of fresh mushrooms, and the lost weight becomes industrial waste water. In China, the raw materials of Agaricus bisporus used for canning processing account for abou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/12A23K10/30A23K10/37A23K10/22A23K20/158A23K20/26C12R1/225C12R1/01C12R1/84
CPCA23K10/12A23K10/30A23K10/37A23K10/22A23K20/158A23K20/26A23K50/30C12N1/20C12N1/16A23V2400/151A23V2400/123A23V2400/51Y02P60/87
Inventor 黄家福
Owner MINNAN NORMAL UNIV
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