A sub-millisecond real-time 3D super-resolution microscopy imaging system

A real-time three-dimensional, microscopic imaging technology, applied in microscopes, analytical materials, material excitation analysis, etc., can solve problems such as inapplicability, unsuitability for rapid molecular motion process monitoring, PSF damage, etc.
CN110231320BActive Publication Date: 2021-06-22FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2021-06-22

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Abstract

The invention belongs to the technical field of single-molecule imaging, and in particular relates to a submillisecond-level real-time three-dimensional super-resolution microscopic imaging system. The system of the present invention comprises: the first objective lens, the second objective lens, the first plane, the second plane, the first plane mirror, the concave mirror, the second plane mirror, and EMCCD; By building the second objective lens, the fluorescence signal on the upper hemisphere is collected to the second plane, and then returned to the sample plane by the concave mirror on the second plane; after being enlarged by the second objective lens, the single molecule signal is separated from the central axis of the concave mirror The distance is close to the focal length of the concave mirror, and the best measurement accuracy in the vertical direction is achieved.
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Description

Technical field

[0001] The present invention belongs to the field of single molecular imaging, and in particular to a mi-miocither real-time three-dimensional ultra-resolution micro-imaging system. Background technique

[0002] Super resolution microscope brings an optical microscope into a nanoscale, achieving visualization of a single molecular passage in living cells. High-time resolution and high-dimensional spatial resolution microscope are widely used to further study the mechanism of cell nuclear pore protein regulatory genes, millisecond magnitude and even faster cilia amimini-molecular transport, cytocnectic molecular exchange and transmembrane channel switch and other biological problems. Therefore, the super resolution microscope experiment system is required to have high-time resolution and high-dimensional spatial distinguishing photoelectric signal detection function.

[0003] Today, the super resolution microscope is mainly divided into two major categories. One ty...

Claims

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