Construction and application of immunocompetent synovial sarcoma xenograft mouse model

Abstract

The invention provides a method for constructing an immune-competent synovial sarcoma xenograft mouse model, which includes the cultivation of the P0 generation: first collect fresh tumor tissue pieces from patients with synovial sarcoma, and then transplant them into immunodeficiency mice under aseptic conditions. In mice; normal immune mice were pretreated with cyclosporine; culture of Px generation: the tumor tissue pieces grown in any generation of mice from P0 generation to Px-1 generation were inoculated into the above-mentioned cells that had been pretreated with cyclosporine. In the treated mice, the Px generation mice were formed; the cultivation of the Py generation: the synovial sarcoma tumor tissue pieces grown in any generation of mice from the above Px generation to the Py-1 generation were inoculated into immune normal mice, until The tumor tissue grows in the body, that is, the cultivation of the Py generation is completed. The invention improves the problem that the traditional PDX model has no immune system, provides an in vivo model for the research of tumor immunotherapy, and broadens the application range of the PDX model.

Description

technical field [0001] The invention relates to the field of establishing a tumor transplantation mouse model, in particular to a construction method and application of an immune-sound synovial sarcoma xenograft mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcomas [1]. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-ha...

AI Technical Summary

Problems solved by technology

However, this method is mainly due to the fact that the immune response caused by the initial antigen stimulation of normal immune mice is generally 7 to 10 days, while the cell-mediated immune response to the allograft is obvious on the 9th to 12th day, so the first 6 days The tumor can proliferate within the tumor, so the main purpose of this method is still to test drug sensitivity, the tumor will still regress, and the observation of the tumor still needs the help of a microscope. unable to proceed

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