Construction method of an immunocompetent synovial sarcoma xenograft mouse model

Abstract

The invention provides a method for constructing an immune-sound synovial sarcoma xenograft mouse model, which includes the cultivation of the P0 generation: first resuscitating the tumor tissue block of a synovial sarcoma patient stored in a liquid nitrogen environment, and then under aseptic conditions It was transplanted into immunodeficient mice; normal immune mice were pretreated with cyclosporine; culture of Px generation: the tumor tissue pieces grown in any generation of mice from P0 to Px‑1 generation were inoculated into the above-mentioned From the mice pretreated with cyclosporine, the Px generation mice were formed; the cultivation of the Py generation: take the synovial sarcoma tumor tissue pieces grown in any generation of mice from the Px generation to the Py-1 generation above and inoculate them into normal immune cells. In the mouse body, until the tumor tissue grows in the body, the culture of the Py generation is completed. The invention improves the problem that the traditional PDX model has no immune system, provides an in vivo model for the research of tumor immunotherapy, and broadens the application range of the PDX model.

Description

technical field [0001] The invention relates to the field of establishment of a tumor transplantation mouse model, in particular to a construction method of an immune-competent synovial sarcoma xenotransplantation mouse model. Background technique [0002] Synovial sarcoma (SS) is a highly malignant soft tissue sarcoma, accounting for about 7-10% of soft tissue sarcomas [1]. Due to the complex origin of SS tissue, no clear risk factors, variable pathological morphology, and difficulty in dividing benign from malignant, its diagnosis, differential diagnosis, and classification have become clinical difficulties. SS is common in men aged 20-30 (male / female ratio is about 1.2:1). It mainly occurs near the large joints of the limbs, but it can occur in almost any part of the body, and SS hardly occurs in the joints, and it is also similar to normal slippery joints. Membrane tissue doesn't matter. About 80% of primary SS occurs in the hands and feet, and about 20% occurs in non-...

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Problems solved by technology

However, this method is mainly due to the fact that the immune response caused by the initial antigen stimulation of normal immune mice is generally 7 to 10 days, while the cell-mediated immune response to the allograft is obvious on the 9th to 12th day, so the first 6 days The tumor can proliferate within the tumor, so the main purpose of this method is still to test drug sensitivity, the tumor will still regress, and the observation of the tumor still needs the help of a microscope. unable to proceed

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