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Application of tdp-43 gene in predicting lactation volume of puerpera

A 1. TDP-43, gene technology, applied in the field of gene application, to achieve the effect of good application prospects

Active Publication Date: 2021-03-09
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method for predicting in advance a woman's lactation potential

Method used

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  • Application of tdp-43 gene in predicting lactation volume of puerpera
  • Application of tdp-43 gene in predicting lactation volume of puerpera
  • Application of tdp-43 gene in predicting lactation volume of puerpera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Correlation analysis between maternal lactation quantity and TDP-43 gene

[0056] Fresh milk samples (3-5 days after delivery) were collected from 60 healthy lactating women. In order to analyze the expression level of TDP-43 in the mammary epithelial cells of the parturients, milk fat components in milk were separated from breast milk by centrifugation, The RNA was extracted and the expression of related genes was detected for gene expression analysis. 42 days after delivery, a questionnaire survey was conducted on the mother's breast milk secretion during lactation. Breast feeding was divided into three groups, exclusive breast feeding, mixed breast feeding and milk formula feeding. The results showed that the expression of TDP-43 was significantly upregulated in the exclusive breastfeeding group compared with the mixed breastfeeding group, while the expression of TDP-43 was lowest in the formula feeding group. These data show that low expression of TDP-43...

Embodiment 2

[0073] Example 2 Effect of TDP-43 Gene on the Quantity and Quality of Lactation in Mice

[0074] In order to further explore how TDP-43 affects the lactation process, the inventors obtained TDP-43 transgenic mice (Tardbp fl / fl )( figure 2 ). Then Tardbp fl / fl Mice were crossed with WAP-Cre transgenic mice. WAP protein is a lactating protein that is specifically expressed in lactating cells during the middle and late stages of pregnancy and lactation (Wagner et al., 1997). The expression of Cre in the mammary gland is initiated by the WAP promoter, which can recognize the flox sites on both sides of the second and third exons of Tardbp and knock out these two exons, resulting in the abnormal coding of TDP-43 protein, and further Obtain mammary gland conditional Tardbp knockout transgenic mice, and specifically study the role of TDP-43 in mammary gland lactation.

[0075] Then observe the effect of knocking out the Tardbp gene on the lactation process. The main function of...

Embodiment 3

[0081] Example 3 Mechanism of TDP-43 Regulating Lactation Quantity and Quality

[0082] Milk fat is stored in the form of lipids wrapped in a three-layer membrane structure in milk. As the main components of the milk fat globule membrane structure, BTN and XOR play an important role in the stability of milk fat globules, the integrity of the membrane structure, and milk fat secretion. Among them, in the Btn1a1 knockout mice, the lipid droplets were enlarged, the milk fat globule membrane structure was incomplete, and milk fat secretion was abnormal (Ogg et al., 2004); in the Xdh heterozygous knockout mice, the lipid droplets were enlarged, Incomplete milk fat globule membrane structure, abnormal intracellular accumulation of milk fat and premature degeneration of the mammary gland (Vorbach et al., 2002). It can be seen that Btn1a1 and Xdh genes play an important role in milk secretion, and the loss of their expression will lead to abnormal milk secretion.

[0083] TDP-43 is a...

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Abstract

The invention provides technical support at the molecular level for predicting maternal lactation, and protects the application of TDP-43 gene in predicting maternal lactation (quantity and quality). Through the research of the present invention, it is expected that the puerpera can know her lactation ability and milk quality in advance, and carry out reasonable and scientific intervention to ensure that the baby has enough floating milk intake and sufficient nutrition, which is beneficial to my country's prenatal and postnatal care policy. It has a good application prospect and lays the foundation for clinical intervention.

Description

technical field [0001] The invention relates to the field of gene application, in particular to the application of TDP-43 gene in predicting maternal lactation (including quantity and quality). Background technique [0002] Today's country vigorously advocates having a second child and breastfeeding. Breast milk plays a vital role in the early stages of infants with its unique nutrition and easy absorption. However, postpartum lactation disorders and insufficient lactation have caused direct damage to the physical and mental health of mothers and infants. It is also not conducive to my country's eugenics and childcare policies. [0003] Mammalian milk can not only provide rich nutrition to feed offspring, but also provide a large number of immune regulatory factors, which play an antibacterial and protective effect on the survival of newborns (Goldman, 2012; Oftedal, 2012). However, there are few studies on the key genes of mammalian milk secretion. In humans, there is grow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6876
CPCC12Q1/6876C12Q2600/158
Inventor 焦保卫赵丽敏柯浩易萍邹丽杨钦
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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