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A strain of Fusobacterium nucleatum obtained from colorectal cancer tumor tissue and its application

A Fusobacterium nucleatum, tumor tissue technology, applied in the direction of tissue culture, tumor/cancer cells, bacteria, etc., to achieve the effect of promoting proliferation

Active Publication Date: 2021-11-23
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diverse Fusobacterium nucleatum strains currently lacking for colorectal cancer research

Method used

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  • A strain of Fusobacterium nucleatum obtained from colorectal cancer tumor tissue and its application
  • A strain of Fusobacterium nucleatum obtained from colorectal cancer tumor tissue and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] THCT14E2 was co-cultured with the colorectal cancer HCT116 cell line, and the specific steps were as follows:

[0029] The cultured HCT116 cells were taken, digested with trypsin to prepare HCT116 cell suspension, counted and inoculated into 24-well plates (1×10 5 cells / well), cultured for 12-24 hours until the cells adhered. After adhering to the wall, wash with PBS and replace the medium without anti-antibody. In the experimental group, freshly prepared THCT14E2 bacteria were added and suspended in PBS to make 1×10 per well 8 CFU; the control group was added with an equal volume of PBS. Three replicate holes were made for each group. cultured under normal conditions. The cell proliferation activity was detected by CCK8 method at 6 hours, 24 hours, 48 ​​hours, and 72 hours of culture respectively. The bacterial suspension was replaced every 24 hours.

[0030] Depend on figure 1 It can be seen that the proliferation activity of HCT116 cells at 72 hours was signif...

Embodiment 2

[0032] THCT14E2 was co-cultured with colorectal cancer LoVo cell line, the specific operation steps are as follows:

[0033] The cultured LoVo cells were taken, digested with trypsin to prepare LoVo cell suspension, counted and inoculated into 24-well plates (1×10 5 cells / well), cultured for 12-24 hours until the cells adhered. After adhering to the wall, wash with PBS and replace the medium without anti-antibody. Freshly prepared THCT14E2 bacteria were suspended in PBS, and the bacterial suspension was added to the experimental group (1×10 8 CFU / well); the control group was added with an equal volume of PBS. Three replicate holes were made for each group. cultured under normal conditions. The cell proliferation activity was detected by CCK8 method at 6 hours, 24 hours, 48 ​​hours, and 72 hours of culture respectively. The bacterial suspension was replaced every 24 hours.

[0034] Depend on figure 2 It can be seen that the proliferation activity of LoVo cells at 72 hou...

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Abstract

The present invention provides a strain of Fusobacterium nucleatum THCT14E2 obtained from colorectal cancer tumor tissue that does not belong to the known subspecies of Fusobacterium nucleatum. The 16SrRNA gene sequence of the strain is specifically shown in SEQ ID NO:1. The taxonomic name of THCT14E2 is Fusobacterium nucleatum, its preservation number is CCTCC NO: M 2019361, the preservation date is May 17, 2019, and the preservation unit is China Center for Type Culture Collection. The results of co-culture of colorectal cancer cell lines with THCT14E2 show that THCT14E2 can significantly promote the proliferation of colorectal cancer cells. Therefore, THCT14E2 can promote the understanding of the diversity of colorectal cancer microecology and will help the development of Fusobacterium nucleatum derived from colorectal cancer. functional research.

Description

technical field [0001] The invention relates to the field of Fusobacterium nucleatum, in particular to a strain of Fusobacterium nucleatum obtained from colorectal cancer tumor tissue and its application. Background technique [0002] Colorectal cancer is a cancer with a high incidence in the world, and the disease burden caused by it has always been a difficult problem that scientists strive to overcome. At present, China has become the country with the largest number of new cases of colorectal cancer each year. Previous epidemiological surveys and experimental studies have generally confirmed that only 5%-15% of the incidence of colorectal cancer is attributed to genetic factors, and the remaining 85% is mainly affected by the environment, such as changes in dietary structure and intestinal microecological imbalance. Researches on colorectal cancer and intestinal microecological disorders are emerging, among which Fusobacterium nucleatum in the intestine is closely relate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N5/09C12R1/01
CPCC12N1/20C12N5/0693C12N2500/72C12N1/205C12R2001/01
Inventor 秦环龙蔚青毕德玺
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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