A manual method for depositing a sample directly onto a slide for liquid based cytology

Abstract

An efficient manual method for preparing a cytology sample for staining. According to the method, a biological sample is provided, wherein the biological sample is a liquid-based cytology sample. A slide is placed in a holder adapted for receiving the slide. The slide is pre-coated with a composition that will cause cells to adhere to the slide. A settling chamber is placed over said slide and thesettling chamber is locked onto the holder. The settling chamber has openings in both proximate and distal ends. The opening in the distal end of the settling chamber is positioned over the slide such that the slide is interposed between the holder and the settling chamber and the slide is in fluid communication with the settling chamber. A density reagent is then dispensed into the settling chamber. The liquid-based cytology sample is then dispensed into the settling chamber over the reagent. The assembly formed by the holder, slide and settling chamber (and its liquid contents) is placed ina centrifuge. The assembly is centrifuged for less than 5 minutes at a rotation force of less than 500g. The density reagent and the liquid thereover are decanted from the settling chamber. The settling chamber is removed from over the slide. The slide is removed from the holder for staining.

Description

[0001] Cross references to related applications [0002] This application claims priority to U.S. Provisional Application No. 62 / 534,841 entitled "Manual Method for Depositing a Sample Directly Onto a Slide for Liquid Based Cytology" filed on July 20, 2017 and incorporated herein by reference. Technical field [0003] The present invention relates to depositing samples on glass slides used in liquid-based cytology (LBC). Background technique [0004] Cancer kills more than 8 million people every year, accounting for approximately 13% of global deaths, most of which come from the Asia-Pacific region. Cytology is already a rapid and minimally invasive form of cancer detection; screening is a rapid, minimally invasive, and widely accepted method, and has reduced the incidence of cervical cancer in countries with organized screening. In the past ten years, several methods have been introduced, namely LBC and molecular testing. For example, current cervical cancer screening cytological...

AI Technical Summary

Problems solved by technology

However, in some parts of the world, automated methods are not feasible due to the high cost of implementation and development

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