A device and method for multi-effect precise electrical stimulation of egg cells

Abstract

The invention discloses a multi-effect precise electric stimulation device for egg cells, comprising: a base; an electric stimulation mechanism, comprising two first electric stimulation components and two second electric stimulation components, each first electric stimulation component includes liquid metal Capillary micro-needle electrodes, a first needle-holding instrument connected to the liquid metal capillary micro-needle electrodes, each second electrical stimulation component comprising a metal parallel-plate micro-electrode and a second needle-holding instrument connected to the metal parallel plate micro-electrodes; The cell pose adjustment chip is installed on the base and used to adjust the cell pose; the rotation mechanism is installed on the base and connected with the cell pose adjustment chip, and is used to drive the cell pose adjustment chip to rotate. The invention also discloses a multi-effect precise electric stimulation method for egg cells. The invention can perform precise electrical stimulation on specific parts of egg cells at different stages in the whole cloning process, realize multiple effects on the targeted electrical stimulation at each stage of the somatic cell cloning process, and improve the success rate of the whole process of somatic cell cloning.

Description

technical field [0001] The invention relates to the field of biotechnology related to cloning, in particular to a multi-effect precise electric stimulation device and method for egg cells oriented to the cloning process. Background technique [0002] Cloning is the process of using biological technology to produce offspring with the same genetic organization as the original individual through asexual reproduction. Since its birth, cloning technology has always occupied the most important position in life science research and related medical technology. Egg cells contain important biological genetic information and are the main objects of operation in the cloning process. However, the success rate of cloning experiments is extremely low. One of the main reasons is that the activity of isolated egg cells will be greatly reduced, and they are very easy to die during the experiment. Even if they do not die during reconstruction, they are also prone to developmental arrest in su...

AI Technical Summary

Problems solved by technology

However, due to the large distance between macroscopic parallel plate electrodes, the voltage required for applying electrical stimulation is also large, which is very easy to cause damage to the cells and is not safe; and the traditional electrical stimulator cannot complete the precise electrical stimulation of specific parts of the egg cells. It is also impossible to use a set of electrical stimulation devices to complete the various electrical stimulations required for cloning egg cells at various stages (electrical activation, electrical fusion, mitochondrial membrane potential increase, etc.)

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