Chicken infectious bursal disease virus multi-epitope protein and application thereof

Abstract

The invention provides a chicken Infectious Bursal Disease Virus (IBDV) multi-epitope protein and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO. 1. A chicken immunoglobulin IgG heavy chain part constant region (gamma CH) is used as a carrier protein of a multi-epitope fusion protein; B cell epitope and T cell epitope genes on IBDV representative strains VP2 andVP3 are subjected to serial synthesis and are then connected with a gamma CH coding gene; a recombinant plasmid pET-VP2 + 3-gamma CH is built; and the recombinant plasmid is converted into escherichiacoli BL21 for expression. The recombinant multi-epitope fusion protein pVP2+ 3-gamma CH is purified and identified and is then subjected to an immune challenge protection test through SPF chicken; and a good immune protection effect is primarily shown. The recombinant multi-epitope protein pVP2+ 3-gamma CH is successfully built and expressed through the study; and the foundation is laid for the research and development of IBDV multi-epitope vaccines.

Description

technical field [0001] The invention relates to a chicken infectious bursal disease virus multi-antigen epitope protein and application thereof, belonging to the field of biotechnology. Background technique [0002] At present, the clinically used livestock and poultry virus disease vaccines are mainly attenuated vaccines and inactivated vaccines. In the clinical application of the attenuated vaccine, there may be safety problems such as strain reversion and gene recombination between the vaccine strain and the wild-type strain. The inactivated vaccine tends to induce humoral immunity, and its ability to induce cellular immunity is relatively weak. An ideal vaccine should not only be safe, but also induce the body's specific humoral immunity and cellular immunity at the same time. By inducing humoral immunity to produce high-affinity neutralizing antibodies, preventing the virus from binding to target cell receptors. Inducing cellular immunity produces specific cytotoxic ...

AI Technical Summary

Problems solved by technology

However, it is worth noting that although conventional vaccines can suppress the occurrence of the disease to a certain extent, there are now some reports of immunization failures, and the outbreak of the disease occurred in chicken farms vaccinated with IBDV vaccines due to immunization failures.

There are many reasons for the failure of immunization, and the difference between the virus itself and the vaccine strain directly leads to the inability of the antibodies produced by immunization to resist the attack of the virus, which is the main reason for the failure of immunization

IBDV is prone to mutation, and many reports of immunization failures indicate that research on new vaccines is urgent

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