Chicken infectious bursal disease virus multi-epitope protein and application thereof

A technology for bursal disease and chicken infectivity, which is applied in the direction of virus antigen components, viruses, and viral peptides, can solve the problems of urgent research on new vaccines, the inability of antibodies to resist viruses, and the tendency to mutate, so as to control virus infection, The effect of good immune effect and low preparation cost

CN112159480AActive Publication Date: 2021-01-01FUJIAN AGRI & FORESTRY UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-01-01

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Abstract

The invention provides a chicken Infectious Bursal Disease Virus (IBDV) multi-epitope protein and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO. 1. A chicken immunoglobulin IgG heavy chain part constant region (gamma CH) is used as a carrier protein of a multi-epitope fusion protein; B cell epitope and T cell epitope genes on IBDV representative strains VP2 andVP3 are subjected to serial synthesis and are then connected with a gamma CH coding gene; a recombinant plasmid pET-VP2 + 3-gamma CH is built; and the recombinant plasmid is converted into escherichiacoli BL21 for expression. The recombinant multi-epitope fusion protein pVP2+ 3-gamma CH is purified and identified and is then subjected to an immune challenge protection test through SPF chicken; and a good immune protection effect is primarily shown. The recombinant multi-epitope protein pVP2+ 3-gamma CH is successfully built and expressed through the study; and the foundation is laid for the research and development of IBDV multi-epitope vaccines.
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Description

technical field

[0001] The invention relates to a chicken infectious bursal disease virus multi-antigen epitope protein and application thereof, belonging to the field of biotechnology. Background technique

[0002] At present, the clinically used livestock and poultry virus disease vaccines are mainly attenuated vaccines and inactivated vaccines. In the clinical application of the attenuated vaccine, there may be safety problems such as strain reversion and gene recombination between the vaccine strain and the wild-type strain. The inactivated vaccine tends to induce humoral immunity, and its ability to induce cellular immunity is relatively weak. An ideal vaccine should not only be safe, but also induce the body's specific humoral immunity and cellular immunity at the same time. By inducing humoral immunity to produce high-affinity neutralizing antibodies, preventing the virus from binding to target cell receptors. Inducing cellular immunity produces specific cytotoxic ...

Examples

Embodiment 1

[0018] 1. Preparation of multi-antigen epitope fusion protein of infectious bursal disease virus

[0019] (1) Prediction and screening of epitopes of infectious bursal disease virus

[0020] Using the ABCpred prediction method combined with the DNAStar software Protean program to predict the epitopes of the VP2 and VP3 proteins of the IBDV reference strains HLJ0504 (GQ451330), Gx (AY444873), YS07 (FJ695138), and E (AF133904) in the GenBank database, Several B-cell antigenic sites and T-cell antigenic sites were screened by combining the two algorithms. The relevant information is shown in Table 1.

[0021] Table 1 Antigen sites related to IBDV multi-epitope protein

[0022]

[0023] The amino acid sequence of the designed multi-antigen epitope protein was analyzed by Protean software, and the results were as follows: figure 1 , the rearranged multi-epitope protein has a higher antigenic index and a higher surface possibility, and can be used for prokaryotic expression to p...