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Tunneling junctions for sequencing

A technology of tunneling junction and sequencing, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, instruments, etc. It can solve problems such as short circuit and high background tunneling current, and achieve the effect of fast manufacturing

Pending Publication Date: 2021-01-29
F HOFFMANN LA ROCHE & CO AG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Also, such a small gap size could create a short circuit and potentially high background tunneling currents

Method used

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  • Tunneling junctions for sequencing
  • Tunneling junctions for sequencing
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Embodiment Construction

[0027] the term

[0028] The term "contact" may refer to bringing one object close to another such that electrons can tunnel from one object through the other. At the subatomic level, two objects may never come into physical contact with each other, because the repulsive forces of the electron clouds in the objects may prevent these objects from coming closer.

[0029] "Nucleic acid" may refer to deoxyribonucleotides or ribonucleotides and polymers thereof in single- or double-stranded form. The term may encompass nucleic acids containing known nucleotide analogs or modified backbone residues or linkages, synthetic, naturally occurring and non-naturally occurring, which have similar binding properties to the reference nucleic acid. Examples of such analogs may include, but are not limited to, phosphorothioate, phosphoramidate, methylphosphonate, 2-O-methylribonucleotide, locked nucleic acid (LNA), peptide nucleic acid (PNA).

[0030] Unless otherwise indicated, a particular ...

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Abstract

Molecules may be analyzed (e.g., sequencing of nucleic acid molecules) by tunneling recognition at a tunneling junction. Embodiments of the present invention may allow detecting individual nucleotidesand the sequencing of a nucleic acid molecule using a tunneling junction. By labeling a specific nucleotide with a moiety, tunneling junctions may generate a signal with a suitable signal-to-noise ratio. The tunneling recognition uses a tunneling current that is mostly through the moiety rather than mostly through the nucleotide or a portion of the molecule of interest. Because a single nucleotide can be detected with a signal with a suitable signal-to-noise ratio resulting from the tunneling current passing through the moiety, embodiments of the present invention may allow for fast detectionof nucleotides using a tunneling current.

Description

Background technique [0001] Techniques for the analysis of single molecules, such as nucleic acids, include tunneling junction devices with a submolecular-sized gap between two conductive layers. Tunneling junctions are identified using tunneling. Tunneling recognition is based on placing molecules or molecular parts, such as nucleotides of nucleic acids, between conductive layers. When a molecule or molecular part touches or is sufficiently close to two layers, the orbitals of the molecule or molecular part will allow electrons to be transferred from one layer to the other, resulting in a tunneling current. Tunneling current can be analyzed to identify molecules or molecular sites. [0002] In order to identify molecular sites, such as nucleotides, the size of the gap will generally have to be on the order of nanometers, including less than 2nm, or even sub-nanometer. Generating such small gaps requires sophisticated and expensive techniques. Reducing the size of the tunn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869
CPCC12Q1/6869C12Q2563/116C12Q2563/157C12Q2565/607C12Q2565/631C12Q2521/101C12Q2535/122C12Q2563/113C12Q1/6876G01N15/1031C12Q2563/155C12Q2525/117G01N15/1023
Inventor Y·阿斯蒂耶F·伯格曼D·海因德尔H·库切尔迈斯特N-P·施滕格勒J·托波兰奇克
Owner F HOFFMANN LA ROCHE & CO AG
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