A kind of plant open tissue culture method and special culture medium

A culture medium and tissue culture technology, applied in the field of plant biology, can solve the problems of restricting the wide application of open tissue culture, difficult toxicity and side effects

Active Publication Date: 2022-03-01
青岛泽欣农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In open tissue culture, the microbial contamination of the medium comes from the microorganisms contained in the medium itself and the microorganisms in the bottle space that settle into the medium. The antibacterial agent of the open tissue culture is dissolved in the agar medium, which is only antibacterial to the part of the medium and does not target The microorganisms in the space in the bottle, so that the antimicrobial agent added to the medium must be kept at a relatively high concentration, which will inevitably produce toxic and side effects on the plant material to be cultivated. This contradiction is the technical difficulty (key) of open tissue culture, which limits the open tissue culture. Wide application of tissue culture

Method used

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  • A kind of plant open tissue culture method and special culture medium
  • A kind of plant open tissue culture method and special culture medium
  • A kind of plant open tissue culture method and special culture medium

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Experimental program
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Effect test

Embodiment 1

[0024] (1) Method

[0025] 1. (1) Medium preparation for the control group (conventional open tissue culture), 200ml culture bottle (cylindrical, bottom diameter 6cm), medium MS + NAA 0.2 mg / L + sucrose 3%, agar 0.6%, antibacterial agent sorbic acid Potassium 0.3% and bromogeramine 0.1% (the concentration of the antibacterial agent is the lowest concentration of open tissue culture medium without microbial contamination), mix with water and boil until completely dissolved, adjust the volume to pH 5.6, and dispense into culture bottles while hot , Each bottle is filled with about 25ml of medium, covered, and placed flat to cool and solidify.

[0026] (2) Oxygen slow-release granules were prepared. The filler was mixed with equal volumes of kaolin and perlite, the adhesive was carboxymethyl cellulose, and the oxygen-releasing agent was sodium percarbonate (Na 2 CO 3 ·3H2 o 2 ), each raw material was pre-treated with ultraviolet light, filler: adhesive: oxygen release agent wa...

Embodiment 2

[0044] (1) Method

[0045] 1. (1) Preparation of open tissue culture medium (control group), 650ml orchid vase (bottom diameter 8cm, bottle mouth diameter about 4cm), medium 1 / 2MS+6-BA3mL / L+sucrose 3%+activated carbon 0.1% + Agar 0.6%. Antibacterial agent sodium hypochlorite 0.3% + bromogeramine 0.2% (the antibacterial agent concentration is the lowest concentration of open tissue culture medium without microbial contamination), mixed with water and heated until completely dissolved, constant volume, adjusted to pH 5.6, and dispensed while hot For culture bottles, fill each bottle with about 100ml of medium, cover it, and place it flat to cool and solidify.

[0046] (2) Oxygen slow-release granules are prepared. The filler is mixed with equal volumes of kaolin and perlite, the adhesive is polyvinyl acetate, and the oxygen-releasing agent is sodium perborate (NaBO 3 4H 2 O), each raw material is pre-cold sterilized, the weight ratio of filler: adhesive: oxygen release agent ...

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PUM

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Abstract

The invention belongs to the technical field of plant biology, and in particular relates to a method and a special culture medium for carrying out open culture in a culture bottle in the open tissue culture of plants. When preparing open tissue culture medium, after the components of the medium are divided into culture bottles, oxygen slow-release granules are put into them, and they are placed flat to cool and solidify. After the medium is inoculated with sprout explants, a layer of non- Volatile oily liquid, open to culture. The invention strengthens the photosynthesis of bottle seedlings, obtains robust bottle seedlings, reduces transpiration, and improves the survival rate of bottle seedlings after transplanting. The invention can also reduce the amount of antimicrobial agents used in culture media and inhibit over-acidification of culture media. The invention has the advantages of low cost, simple preparation, convenient application and remarkable effect.

Description

technical field [0001] The invention belongs to the field of plant biotechnology. The invention relates to a plant open tissue culture method and a special culture medium, in particular to a method for improving the gas microenvironment of bottle seedlings in plant open tissue culture. Background technique [0002] Plant tissue culture technology has broad market prospects for seedling production, virus removal, and germplasm improvement. The open tissue culture of plants (open tissue culture) that has attracted widespread attention in recent years is to add antibacterial agents to the medium without autoclaving, inoculate in an open environment (no ultra-clean bench) and cover for conventional culture. Open tissue culture is of great significance in improving operational efficiency and reducing tissue culture costs. Related papers (Liu Fuping. Research methods for the application of antibacterial agents in plant antibacterial tissue culture. Genomics and Applied Biology, 2...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 刘泽洋
Owner 青岛泽欣农业科技有限公司
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