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A kind of buffer and its application in neuron-specific enolase detection kit

A technology of enolase and detection reagents, applied in neuron-specific enolase detection kits, in the field of buffer solutions, can solve the problem of incompatibility between detection process and sensitivity

Active Publication Date: 2022-01-14
SOPHONIX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are situations in which the detection process and sensitivity cannot be compatible in the prior art

Method used

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  • A kind of buffer and its application in neuron-specific enolase detection kit
  • A kind of buffer and its application in neuron-specific enolase detection kit
  • A kind of buffer and its application in neuron-specific enolase detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] A buffer solution, in parts by weight, the buffer solution includes the following components: Tris 4.2g, KCl 8.5g, disodium hydrogen phosphate dodecahydrate 5.8g, magnesium sulfate 5g, trehalose 10g , BSA 15g.

[0075] The preparation method of described buffer solution, comprises the following steps:

[0076] (1) Weigh Tris, KCl, NaH 2 PO 4 , MgSO 4 , trehalose, BSA, add to purified water and stir until completely dissolved, adjust the pH value to 7.2, and set the volume to 1000ml;

[0077] (2) Filter with a 0.22 μm filter membrane to obtain the buffer solution. (Denoted as buffer S1)

Embodiment 2

[0079] A buffer solution, in parts by weight, the buffer solution includes the following components: 15.6 g of trishydroxymethylaminomethane, 24 g of KCl, 1.2 g of disodium hydrogen phosphate dodecahydrate, 0.1 g of magnesium sulfate, trehalose 50g, BSA 1.0g.

[0080] The preparation method of described buffer solution, comprises the following steps:

[0081] (1) Weigh Tris, KCl, NaH 2 PO 4 , MgSO 4 , trehalose, BSA, add to purified water and stir until completely dissolved, adjust the pH value to 6.8, and set the volume to 1000ml;

[0082] (2) Filter with a 0.22 μm filter membrane to obtain the buffer solution. (Denoted as buffer S2)

Embodiment 3

[0084] A buffer solution, which comprises the following components in parts by weight: 20 g of trishydroxymethylaminomethane, 6 g of KCl, 6 g of disodium hydrogen phosphate dodecahydrate, 10 g of magnesium sulfate, 5.0 g of trehalose, and 50 g of BSA.

[0085] Preferably, the preparation method of the buffer comprises the following steps:

[0086] (1) Weigh Tris, KCl, NaH 2 PO 4 , MgSO 4 , trehalose, BSA, add to purified water and stir until completely dissolved, adjust the pH value to 8.5, and set the volume to 1000ml;

[0087] (2) Filter with a 0.22 μm filter membrane to obtain the buffer solution. (Denoted as buffer S3)

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Abstract

The invention belongs to the technical field of immunodiagnosis, and in particular relates to a buffer solution and its application in a neuron-specific enolase detection kit. The buffer includes the following components: Tris, KCl, disodium hydrogen phosphate dodecahydrate, magnesium sulfate, trehalose, and BSA. The buffer solution can be used to prepare a kit for detecting NSE, the kit can quickly and easily detect the content of neuron-specific enolase in human peripheral blood, the operation is simple, and it can be used for small cell lung cancer, neuroblastoma, Wilms tumor provides an in vitro diagnostic kit for auxiliary diagnosis and its preparation method and use.

Description

technical field [0001] The invention belongs to the technical field of kit preparation, and in particular relates to a buffer solution and its application in a neuron-specific enolase detection kit. Background technique [0002] Neuron-specific enolase (Neuron-Specific Enolase, NSE) is an acidic protease with a molecular weight of about 78,000. NSE is one of the important markers of small cell lung cancer, which can be used for differential diagnosis and to detect the therapeutic effect of small cell lung cancer after radiotherapy and chemotherapy. NSE can also be used in the differential diagnosis of neuroblastoma and Wilms tumor, and has high clinical application value in the early diagnosis of neuroblastoma. [0003] There are many detection methods in the prior art, including ELISA, FIA, plate chemiluminescence and other methods, but the sensitivity and stability of NSE in ELISA, FIA, plate chemiluminescence and other methodologies have been unsatisfactory, which has se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/573G01N33/543G01N33/535
CPCG01N33/57488G01N33/57423G01N33/57407G01N33/57438G01N33/573G01N33/535G01N33/54326G01N2333/988
Inventor 王法龙刘聪魏宏娟李博飞
Owner SOPHONIX CO LTD
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