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Breeding method of a new high-yield, lodging-resistant and disease-resistant wheat variety
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A new variety, wheat technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problems of loose plant type, easy lodging, affecting high yield, etc.
Active Publication Date: 2022-07-29
JIANGSU LIXIAHE REGION AGRI RES INST
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[0004] The biggest disadvantage of Yangmai 18 is that the stalk has poor elasticity or toughness, the plant type is loose, and it is easy to lodging, which affects its high yield.
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Embodiment 1
[0027] Embodiment 1 Breeding method of a new high-yield, lodging-resistant and disease-resistant wheat variety
[0028] 1) At the beginning of January 2015, the dwarf and large panicle stalks were planted in the greenhouse as an intermediate material of lodging-resistant wheat, Yuanyou-2 and the scab-resistant wheat variety Yangmai 18. In April, Yuanyou-2 was used as the female parent, and the Yangmai 18 is the male parent, a hybrid combination is prepared, and a hybrid F1 is obtained at the end of May;
[0029] The intermediate material of dwarf stalk and large ear lodging resistant wheat is Yuanyou-2, which is an intermediate material with dwarf stalk, large ear and hard stalk provided by the research group of Mr. Li Lihui of the Chinese Academy of Agricultural Sciences during the implementation of the 973 project. It is planted in the Institute of Crop Science, Chinese Academy of Agricultural Sciences. The national uniform number is ZM017976. According to investigation, it ...
Embodiment 2
[0039] Example 2: Establishment of the QGns-YY-5B / QGbs-YY-5B genotype method for molecular marker-assisted selection of the number of grains per ear and the number of grains set at the base of the ear:
[0040] Use the molecular marker Xgpw4483 primer set to detect the materials in step 5 and step 6 in Example 1 respectively;
[0041] 1. Use CTAB method (Reference: Stacey J, Isaac PG. Isolation of DNA from plants. Methods Mol. Biol. 1994, 28: 9-15) to extract the F3 individual plant in step 5 and the F4 plant in step 6 at the seedling stage The mixed leaf genomic DNA of the row was diluted to obtain a template solution with a DNA concentration of about 30 ng / μL.
[0042] 2. According to the existing references (Hu Wenjing, Gao Derong, Lu Chengbin, etc., QTL mapping of wheat panicle traits and plant height and analysis of T6VS.6AL translocation effect, Journal of Wheat Crops, 2019, 39(5): 505- 512), the number of grains per ear QGns-YY-5B and the number of grains set at the ba...
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Abstract
The invention discloses a breeding method for a new high-yield, lodging-resistant and disease-resistant wheat variety. The method uses a lodging-resistant wheat intermediate material with a dwarf and large panicle stem as the female parent, and a disease-resistant variety Yangmai 18 is used as the male parent to cross once , backcross once, and then add generation in the greenhouse. In the process of adding generation, powdery mildew is induced, and individual plants against powdery mildew are selected, and then combined with the number of grains per ear and the number of grains at the base of the ear, the synergistic site QGns‑YY‑5B / QGbs‑YY‑ Molecular marker selection was carried out in 5B, and the selected lines were planted in the field, artificially induced and selected for powdery mildew and scab in high generations, and comprehensive agronomic traits, stalk toughness, lodging resistance and disease resistance were selected. The new wheat variety bred by the method of the invention stably inherits the resistance to scab and powdery mildew of Yangmai 18, and adds the excellent characters of good stem toughness and lodging resistance of the non-recurrent parent, and the average yield is significantly higher than that of Yangmai 18. .
Description
technical field [0001] The invention belongs to the technical field of crop breeding methods, and relates to a breeding method for a new high-yield, lodging-resistant and disease-resistant wheat variety. Background technique [0002] The rice stubble and wheat areas in the middle and lower reaches of the Yangtze River in my country are the frequent and recurring areas of scab. On the other hand, the fungus will overwinter on the rice stumps, resulting in a long-term source of scab infection in the planting area. From 2012 to 2015, the average annual occurrence area of scab in Jiangsu Province was about 1.2 million hectares 2 . The infection period of head blight is mainly during the flowering period of wheat. After the gibberella infects the florets, it expands rapidly in the ear, and it reproduces continuously during the grain filling and maturation of wheat, and then accumulates various toxins, such as deoxynivalenol. (deoxynivalenol, DON), nivalenol (nivalenol, NIV) a...
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