Rabbit semen cryopreservation diluent, preparation method, rabbit semen cryopreservation method and application

A technique for cryopreservation and diluent, which is applied to rabbit semen cryopreservation diluent and preparation, rabbit semen cryopreservation method and application field, and can solve the problems of low vitality, uneven quality, difficulty in artificial insemination, production and reproduction, etc. question

Active Publication Date: 2022-02-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are almost no commercial products of rabbit semen cryopreservation diluent in the market, and the quality is uneven. The vita

Method used

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  • Rabbit semen cryopreservation diluent, preparation method, rabbit semen cryopreservation method and application
  • Rabbit semen cryopreservation diluent, preparation method, rabbit semen cryopreservation method and application
  • Rabbit semen cryopreservation diluent, preparation method, rabbit semen cryopreservation method and application

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preparation example Construction

[0028] The present invention provides the preparation method of the rabbit semen cryopreservation diluent described in the above technical scheme, comprising the following steps: trishydroxymethylaminomethane, citric acid, glucose, trehalose, L-glutamic acid, dimethyl Sulfoxide, glycerol and water mixed in the system. The present invention has no special requirements on the method of mixing, and the conventional mixing method in the field can be used to mix evenly. After mixing in the present invention, the rabbit semen cryopreservation diluent of the present invention is obtained. In the present invention, there is no special storage condition for the rabbit semen cryopreservation diluent, and low temperature storage can be used. In the present invention, the temperature of the cryopreservation is preferably 2-8°C, more preferably 4°C. The rabbit semen cryopreservation diluent provided by the invention has a simple preparation method, easy quality control and is suitable fo...

Embodiment 1

[0039] A diluent for cryopreservation of rabbit semen, added to every 100 mL of purified water: 3.03 g of tris, 1.69 g of citric acid, 0.85 g of glucose, 16 mL of dimethyl sulfoxide, 2.05 g of trehalose, and L-glutamic acid 0.74g and 3mL of glycerol, pH 7.3.

[0040] Preparation method: Add the raw materials of the above components into purified water, stir thoroughly, mix well, and store at 4°C.

Embodiment 1-1

[0042] A diluent for cryopreservation of rabbit semen, added to every 100 mL of purified water: 3.4 g of tris, 1.5 g of citric acid, 0.7 g of glucose, 14 mL of dimethyl sulfoxide, 2.2 g of trehalose, and L-glutamic acid 0.7g and 2.5mL of glycerol, pH 7.2.

[0043] The preparation method is the same as in Example 1.

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Abstract

The invention provides a rabbit semen cryopreservation diluent, a preparation method, a rabbit semen cryopreservation method and application, and belongs to the technical field of artificial insemination of livestock. The invention provides a rabbit semen cryopreservation diluent. The rabbit semen cryopreservation diluent takes water as a solvent. In the rabbit semen cryopreservation diluent, 3-4 g of trihydroxymethyl aminomethane, 1-2 g of citric acid, 0.5-1 g of glucose, 2-3 g of trehalose, 0.5-1 g of L-glutamic acid, 10-20 mL of dimethyl sulfoxide and 2-4 mL of glycerol are added into every 100 mL of water; and the pH value of the rabbit semen cryopreservation diluent is 6.5-7.5. The rabbit semen cryopreservation diluent provided by the invention obviously improves the motility rate, plasma membrane integrity rate and acrosome integrity rate of frozen sperms, and is beneficial to improving the breeding efficiency and breeding speed of rabbits.

Description

technical field [0001] The invention belongs to the technical field of livestock artificial insemination, and in particular relates to a diluent for cryopreservation of rabbit semen and a preparation method thereof, a method for cryopreservation of rabbit semen and an application thereof. Background technique [0002] Rabbits are multi-fetal animals, and fresh semen can basically meet the daily needs of artificial insemination. However, for the long-distance transportation of breeding male rabbits, frozen semen can avoid the problems of quarantine, isolation and breeding of breeding animals when live animals are introduced. Moreover, sperm freezing for endangered strains and transgenic strains is more convenient and simpler than embryo preservation. At the same time, the semen cryopreservation technology can preserve the semen of male rabbits of fine breeds for a long time, so as to effectively alleviate the problems caused by the "stuck neck" of germplasm resources. The p...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221Y02A50/30
Inventor 邵乐潘孝青翟频秦枫杨杰张霞李健
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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