Two-channel spectrum detection device for capillary electrophoresis

Abstract

The invention relates to the field of electrophoresis detection, and particularly discloses a capillary electrophoresis two-channel spectrum detection device which comprises an electrophoresis capillary, and a first detection mechanism and a second detection mechanism are arranged on the outer side of the electrophoresis capillary. The first detection mechanism and the second detection mechanism are located on the outer side of the electrophoresis capillary tube and are perpendicular to each other, and the ends, close to the electrophoresis capillary tube, of the first detection mechanism and the second detection mechanism are connected with a second light condensation sheet. The spectrum analyzer and the laser are used for detecting micro samples and trace samples, so that the sensitivity is improved, and the experiment reliability is improved; detection targets are expanded, and the working efficiency of the instrument is improved; the fluorescence cross is reduced, and the experiment accuracy is improved; the manufacturing cost of the dye kit is reduced, detection items are increased, laser and fluorescence signals are transmitted to the capillary tube and the spectrum analyzer through the optical fiber, and the detection accuracy can be conveniently controlled.

Description

technical field [0001] The invention relates to the field of electrophoresis detection, in particular to a dual-channel spectral detection device for capillary electrophoresis. Background technique [0002] The existing international standard technology adopts single laser, single detector and multi-fluorescence capillary gene detection method, which is currently the gold standard that can be achieved in clinical medicine, judicial organs, agricultural research and other fields. Because a single laser is used to excite multiple fluorescent dyes, it is difficult to excite more than six different fluorescent dyes in this way, and the maximum number of sites that can be excited is 24; but now all the items that need to be tested, the sites that need to be excited Generally, it is 60-100 bits, so the existing technology can no longer satisfy the market application. SUMMARY OF THE INVENTION [0003] In view of the existing problems, the present invention provides a dual-channe...

AI Technical Summary

Problems solved by technology

Because a single laser is used to excite multiple fluorescent dyes, it is difficult to excite more than six different fluorescent dyes by this method, and the maximum number of sites that can be excited is 24; but now in all the items that need to be tested, the sites that need to be excited Generally in the 60-100 position, so the existing technology can no longer satisfy the market application

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