Traditional Chinese medicine oral liquid for treating damp-heat diarrhea of pigs and chickens and preparation method of traditional Chinese medicine oral liquid

Abstract

The invention provides a traditional Chinese medicine oral liquid for treating damp-heat diarrhea of pigs and chickens and a preparation method thereof. The traditional Chinese medicine oral liquid for treating damp-heat diarrhea of pigs and chickens comprises the following components: a traditional Chinese medicine extracting solution, a poly-combined accelerant and auxiliary materials, the formula of the traditional Chinese medicinal materials with different effects is optimized, so that the traditional Chinese medicinal oral liquid has the effects of clearing away heat and toxic materials, cooling blood and stopping dysentery, and tonifying spleen and nourishing stomach, the taste of the medicine and the dispersion state of the medicine in a solvent are improved through the self-made poly-combined accelerant and auxiliary materials, the absorption of the medicine is promoted, the medicine effect is accelerated, and the medicine resistance is not generated.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese veterinary medicines, and in particular to a traditional Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens and a preparation method thereof. Background Art

[0002] Damp-heat diarrhea is one of the common diseases in pig and chicken farming, which has a serious impact on the health and growth of pigs and chickens, and brings certain economic losses to the farming industry. The incidence of damp-heat diarrhea is relatively common in pigs and chickens. Sick pigs and chickens often show symptoms such as diarrhea, abnormal feces, and lack of energy. In severe cases, it can lead to dehydration, weight loss, and even death. Environmental factors: A high temperature and high humidity breeding environment is conducive to the breeding of damp heat. Improper feeding management: poor feed quality, unhygienic drinking water, etc. Pathogen infection: such as infection by certain bacteria and viruses.

[0003] At present, drugs are mainly used to treat damp-heat diarrhea in pigs and chickens. Common therapeutic drugs include antibiotics, traditional Chinese medicine, etc. However, long-term and large-scale use of antibiotics may lead to drug resistance problems. The frequent occurrence of damp-heat diarrhea not only affects the growth performance and production benefits of pigs and chickens, but also increases the breeding costs, including treatment costs, losses caused by slow growth or death due to the disease, etc. Due to certain limitations of traditional treatment methods, there is a great demand in the market for safer and more effective treatment options, especially green and environmentally friendly traditional Chinese medicine treatment options. With the emphasis on healthy breeding, more attention will be paid to strengthening preventive measures, improving the breeding environment and feeding management in the future. At the same time, the research and application of natural medicines such as traditional Chinese medicine will continue to deepen in order to seek more sustainable treatment methods.

[0004] At present, the patent with the patent number "CN1857436A" discloses a Chinese medicine composition for clearing away heat and detoxifying, drying dampness and stopping diarrhea, as well as its preparation method and application. The effective ingredients of the Chinese medicine composition are made from the following raw materials in a weight ratio: 20-30 parts of berberine hydrochloride, 300-450 parts of celandine, 300-450 parts of pulsatilla, and 300-450 parts of fraxinus. The structural components of the Chinese medicine composition are relatively simple, and if taken for a long time, poultry will produce antibodies, resulting in unsatisfactory prevention and treatment effects.

[0005] In view of the problems existing in the prior art, the purpose of this application is to provide a Chinese medicine oral liquid that is quickly absorbed and has the effects of clearing away heat and detoxifying, cooling blood and stopping diarrhea, and nourishing the spleen and stomach. Summary of the invention

[0006] Purpose of the invention:

[0007] The purpose of the present invention is to provide a Chinese medicine oral liquid which is quickly absorbed and has the effects of clearing away heat and detoxifying, cooling blood and stopping dysentery, and nourishing the spleen and stomach.

[0008] The technical solution of the present invention:

[0009] The present application invents a Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, characterized in that the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens comprises the following components: a Chinese medicine extract, a polymer combined promoter, auxiliary materials, and deionized water.

[0010] Furthermore, the Chinese medicine extract comprises the following components by weight: 200-300 parts of Scutellaria baicalensis, 280-350 parts of Gardenia jasminoides, 120-200 parts of Qinpi, 120-200 parts of Pulsatilla scutellariae, 180-300 parts of Radish seeds, 200-400 parts of malt, 175-250 parts of Atractylodes macrocephala, 100-200 parts of Dioscorea opposita, 100-150 parts of Codonopsis pilosula, and 100-120 parts of Costusroot.

[0011] Furthermore, the auxiliary materials include the following components: sucrose, sorbic acid, and sodium alginate.

[0012] Furthermore, the raw materials for preparing the polymer combined accelerator include: linolenic acid, propylene oxide, dimethyl ethylene oxide, and zinc oxide.

[0013] The preparation method of the polymeric combined accelerator comprises the following steps:

[0014] S1: Add linolenic acid into the reactor, control the temperature to 100-120°C, and stir slowly;

[0015] S2: slowly drip propylene oxide and dimethyl ethylene oxide into the reaction kettle of step S1, stir for 15 to 30 minutes, then add zinc oxide to react for 2 to 6 hours to obtain a crude product of a polymerized combined accelerator;

[0016] S3: extracting and drying the crude product of the polymerized combined accelerator to obtain the polymerized combined accelerator.

[0017] Furthermore, the total mass ratio of the linolenic acid to propylene oxide and dimethylethylene oxide is 2 to 5:1.

[0018] Furthermore, the weight average molecular weight of the polymeric combined promoter is 5000 to 12000 Da.

[0019] Furthermore, the added amount of zinc oxide is 1 to 2.5% of the total mass of linolenic acid, propylene oxide and dimethylethylene oxide.

[0020] The method for preparing the Chinese medicine extract comprises the following steps:

[0021] S1: crush and grind Scutellaria baicalensis, Gardenia jasminoides, Cortex qinpi, Aucklandia lappa, Pulsatilla chinensis, Radish seeds, Malt, Atractylodes macrocephala, Dioscorea opposita, and Codonopsis pilosula for 30 to 60 minutes respectively;

[0022] S2: Weigh Scutellaria baicalensis, Gardenia jasminoides, Cortex qinpi, Aucklandia lappa, Pulsatilla chinensis, Radish seeds, Malt, Atractylodes macrocephala, Dioscorea opposita, and Codonopsis pilosula, put them into a casserole, stir-fry over low heat for 15 to 30 minutes, add ethanol and water, and boil for 2 to 4 hours;

[0023] S3: After decocting, the filtrate obtained by filtration is collected to prepare a Chinese medicine extract.

[0024] Furthermore, the mass ratio of the traditional Chinese medicine extract to the polymer combined promoter is 8 to 12:1.

[0025] Furthermore, the auxiliary material accounts for 10-15% of the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens.

[0026] The present application also provides a method for preparing a Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, which is characterized by comprising the following steps:

[0027] Step 1: Place the Chinese medicine extract in a container, control the temperature at 60-70°C, and concentrate the Chinese medicine extract to a concentrated solution with a relative density of 1.2-1.5;

[0028] Step 2: Add auxiliary materials and polymer joint promoter to the concentrated solution respectively, stir evenly, and obtain a Chinese medicine mixture;

[0029] Step 3: prepare the Chinese medicine mixture with deionized water into oral liquids of different concentrations, and then pasteurize and fill them to obtain the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens.

[0030] The invention provides a Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, which adopts a Chinese medicine formula, is safe and non-toxic, and will not produce drug resistance. Among them, Radix Scutellariae, Fructus Gardeniae, Rhizoma Pulsatillae, and Cortex Fraxini have the effects of clearing away heat and dampness, purging fire and detoxifying, cooling blood and stopping diarrhea, and have good therapeutic effects on diarrhea, dysentery, and blood in stool caused by damp-heat in the gastrointestinal tract; Radish, Atractylodes macrocephala, Dioscorea opposita, and Codonopsis pilosula can promote qi and relieve pain, strengthen the spleen and digest food, dry dampness and promote diuresis, and can be used for abdominal pain caused by qi stagnation and strengthen the spleen and stomach; Radish seeds and malt can digest food and eliminate bloating, descend qi and resolve phlegm, help digest food, and increase appetite.

[0031] The hydrophilic group (-O-) of the homemade polymer co-accelerator gives it water solubility, and the hydrophobic group (alkyl) interacts with the hydrophobic part of the drug to help the drug dissolve, maintain the stability of the drug solution, prevent the drug from precipitating or settling, reduce the interfacial tension of the drug solution, increase the contact area between the drug and the absorption site, thereby promoting the absorption of the drug and accelerating the efficacy.

[0032] Beneficial effects:

[0033] The invention provides a Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens. The liquid has fast absorption, fast drug effect, no drug resistance, the effects of clearing away heat and detoxifying, cooling blood and stopping diarrhea, and nourishing the spleen and stomach, and can also increase the appetite of pigs and chickens. The liquid has a wide prospect of promotion and application in the technical field of Chinese veterinary medicine. DETAILED DESCRIPTION

[0034] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, but not to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.

[0035] Linolenic acid was purchased from Xuchang Ruixinfu Information Technology Co., Ltd.; dimethylethylene oxide was purchased from Shandong Tianmao New Materials Technology Co., Ltd.; Tween-80 and poloxamer were purchased from Guangzhou Warner Biotechnology Co., Ltd.

[0036] Unless otherwise specified, the chemical reagents used in the present invention are all commercially available analytically pure.

[0037] Preparation of Polymeric Combined Accelerator A:

[0038] S1: Add 5 g of linolenic acid into the reactor, control the temperature to 120°C, and stir slowly;

[0039] S2: Slowly drop 1g of propylene oxide and 1g of dimethylethylene oxide into the reaction kettle of step S1, stir for 15 minutes, then add 70mg of zinc oxide to react for 4 hours to obtain a crude product of a polymerized combined accelerator;

[0040] S3: The crude polymeric combined accelerator was extracted with dichloromethane and dried at 45° C. to obtain a polymeric combined accelerator A, the weight average molecular weight of which was 6542 Da.

[0041] Preparation of Polymeric Combined Accelerator B:

[0042] The difference between this preparation and the polymeric combined accelerator A is that 5 g of linolenic acid and 70 mg of zinc oxide are replaced by 8 g of linolenic acid and 100 mg of zinc oxide, respectively, and the weight average molecular weight of the polymeric combined accelerator B is 11450 Da.

[0043] Preparation of Polymeric Combined Accelerator C:

[0044] The difference between this preparation and the polymeric combined accelerator A is that 70 mg of zinc oxide is replaced by 200 mg of zinc oxide, and the weight average molecular weight of the polymeric combined accelerator C is 13562 Da.

[0045] Preparation of Polymeric Combined Accelerator D:

[0046] The difference between this preparation and the polymeric combined accelerator A is that 70 mg of zinc oxide is replaced by 20 mg of zinc oxide, and the weight average molecular weight of the polymeric combined accelerator D is 3578 Da.

[0047] Preparation of Polymeric Combined Accelerator E:

[0048] The difference between this preparation and the polymeric combined accelerator A is that 5 g of linolenic acid and 70 mg of zinc oxide are replaced by 12 g of linolenic acid and 140 mg of zinc oxide, respectively, and the weight average molecular weight of the polymeric combined accelerator E is 7864 Da.

[0049] Preparation of Polymeric Combined Accelerator F:

[0050] The difference between this preparation and the polymeric combined accelerator A is that 5 g of linolenic acid and 70 mg of zinc oxide are replaced by 3 g of linolenic acid and 50 mg of zinc oxide, respectively, and the weight average molecular weight of the polymeric combined accelerator F is 5784 Da.

[0051] Preparation of Chinese medicine extract A:

[0052] S1: crush and grind Scutellaria baicalensis, Gardenia jasminoides, Cortex qinpi, Aucklandia lappa, Pulsatilla chinensis, Radish seeds, Malt, Atractylodes macrocephala, Dioscorea opposita, and Codonopsis pilosula for 50 minutes respectively;

[0053] S2: Weigh 25g Scutellaria baicalensis, 30g Gardenia jasminoides, 15g Fragrant cortex, 10g Aucklandia lappa, 15g Pulsatilla chinensis, 30g Radish seeds, 40g Malt, 20g Atractylodes macrocephala, 20g Dioscorea opposita, and 10g Codonopsis pilosula, put them into a casserole, stir-fry over low heat for 20 minutes, add 200ml ethanol and 1L water, and simmer for 4 hours;

[0054] S3: After decoction, the filtrate obtained by filtration is collected to prepare the Chinese medicine extract A.

[0055] Preparation of Chinese medicine extract B:

[0056] The difference between the present preparation and the Chinese medicine extract A is that no costusroot, atractylodes, yam or codonopsis is added in step S2.

[0057] Preparation of Chinese medicine extract C:

[0058] The difference between the present preparation and the Chinese medicine extract A is that radish seeds and malt are not added in step S2.

[0059] Preparation method of Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens:

[0060] Step 1: Place the Chinese medicine extract in a container, control the temperature at 65°C, and concentrate the Chinese medicine extract to a concentrated solution with a relative density of 1.2;

[0061] Step 2: Add sucrose, sorbic acid, sodium alginate and a polymer joint promoter to the concentrated solution in sequence, stir evenly, and obtain a Chinese medicine mixture;

[0062] Step 3: prepare the Chinese medicine mixture with deionized water into a 1 mg / ml oral solution, and then pasteurize and fill it to obtain the Chinese medicine oral solution for treating damp-heat diarrhea in pigs and chickens.

[0063] Examples 1 to 11

[0064] According to the above-mentioned preparation method of the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, Table 1 is the ingredient list of the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens of Examples 1 to 11.

[0065] Table 1

[0066] Comparative Examples 1 to 3

[0067] Comparative Examples 1 to 3 were prepared according to the above-mentioned preparation method of the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens. Table 2 is the ingredient list of the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens of Comparative Examples 1 to 3.

[0068] Table 2

[0069]

[0070] Test experiment:

[0071] The 20-day-old chicks were randomly divided into 15 groups, with 20 chicks in each group. One day in advance, the chicks were orally inoculated with 5×10 sporulated oocysts of Eimeria tenella. 4 A blank control group (no insect attack and no medication) was set up, and a medication group was set up, with a total of 14 groups, which were respectively Huangzhi oral liquid prepared in Examples 1 to 11 and Comparative Examples 1 to 3. The chicks in the medication group were given 2 ml of the drug once a day, and the test period was 10 days.

[0072] The chicks in each group were observed clinically for 10 days, and all the chickens were weighed, and the relative weight gain rate, survival rate, and bloody stool scores were recorded.

[0073] 1.Relative weight gain rate:

[0074] Relative weight gain rate (%) = average weight gain of the infected and medicated group / average weight gain of the uninfected and unmedicated group × 100%.

[0075] 2. Survival rate:

[0076] Survival rate (%) = (total number of chickens in the group - number of chickens in each group that died due to coccidia infection) / total number of chickens in the group × 100%.

[0077] 3. Scoring of bloody stools:

[0078] On the 4th, 6th, 8th and 10th days after inoculation of oocysts, refer to the method of Morehouse (1970) and score according to the following standards: 0: 100% feces without blood; 1: 1-25% feces with blood; 2: 26-50% feces with blood; 3: 51-75% feces with blood; 4: 76-100% feces with blood. Scoring bloody stools can be used to evaluate the speed of the effect of traditional Chinese medicine.

[0079] Table 3:

[0080]

[0081] Table 4: Bloody stool scores of chicks in different experimental groups

[0082]

[0083] From the above data, it can be seen that the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens prepared by the present invention has a fast effect, good therapeutic effect, and a high cure survival rate. Specifically, compared with Example 2, it can be seen that when preparing the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, the unreasonable mass ratio of the Chinese medicine extract and the polymer combined promoter will lead to a slow effect speed of the prepared Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, and the therapeutic effect is not significantly improved; compared with Example 2, it can be seen that when preparing the Chinese medicine extract, the lack of several Chinese medicines will lead to a lower cure survival rate of the prepared Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, a slower effect speed, and an insignificant increase in weight; compared with Example 2, it can be seen that when preparing the polymer combined promoter, the distribution of the polymer combined promoter is Too much or too little amount of the accelerator will lead to a slower onset of action, insignificant weight gain and poor therapeutic effect of the prepared Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens; compared with Example 2, Examples 10 to 11 show that the ratio of linolenic acid, propylene oxide and dimethyl ethylene oxide in the polymer combined accelerator is unreasonable, which will lead to a slower onset of action, insignificant weight gain and poor therapeutic effect of the prepared Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens; compared with Comparative Example 1 and Example 1, it can be seen that the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens without adding the polymer combined accelerator has a slower onset of action and a poor therapeutic effect; compared with Comparative Examples 2 to 3 and Example 2, it can be seen that the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens using different accelerators has a slower onset of action and a poor therapeutic effect, and the expected effect cannot be achieved.

[0084] The present invention can also have many other embodiments. Without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and modifications based on the present invention, but these corresponding changes and modifications should all fall within the scope of protection of the claims attached to the present invention.

Claims

1. A Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens, characterized in that: The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens comprises the following components: Chinese medicine extract, a polymer joint accelerator, auxiliary materials, and deionized water.

2. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 1, characterized in that: The Chinese medicine extract comprises the following components by weight: 200-300 parts of Scutellaria baicalensis, 280-350 parts of Gardenia jasminoides, 120-200 parts of Qinpi, 120-200 parts of Pulsatilla scutellariae, 180-300 parts of Radish seeds, 200-400 parts of malt, 175-250 parts of Atractylodes macrocephala, 100-200 parts of Dioscorea opposita, 100-150 parts of Codonopsis pilosula, and 100-120 parts of Costusroot.

3. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 1, characterized in that: The auxiliary materials include the following components: sucrose, sorbic acid and sodium alginate.

4. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 1, characterized in that: The raw materials for preparing the polymer combined accelerator include: linolenic acid, propylene oxide, dimethyl ethylene oxide and zinc oxide.

5. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 4, characterized in that: The preparation method of the polymeric combined accelerator comprises the following steps: S1: Add linolenic acid into the reactor, control the temperature to 100-120°C, and stir slowly; S2: slowly drip propylene oxide and dimethyl ethylene oxide into the reaction kettle of step S1, stir for 15 to 30 minutes, then add zinc oxide to react for 2 to 6 hours to obtain a crude product of a polymerized combined accelerator; S3: extracting and drying the crude product of the polymerized combined accelerator to obtain the polymerized combined accelerator.

6. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 4, characterized in that: The total mass ratio of the linolenic acid to propylene oxide and dimethylethylene oxide is 2 to 5:

1.

7. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 4, characterized in that: The weight average molecular weight of the multi-polymer joint promoter is 5000-12000Da.

8. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 1, characterized in that: The method for preparing the Chinese medicine extract comprises the following steps: S1: crush and grind Scutellaria baicalensis, Gardenia jasminoides, Cortex qinpi, Aucklandia lappa, Pulsatilla chinensis, Radish seeds, Malt, Atractylodes macrocephala, Dioscorea opposita, and Codonopsis pilosula for 30 to 60 minutes respectively; S2: Weigh Scutellaria baicalensis, Gardenia jasminoides, Cortex qinpi, Aucklandia lappa, Pulsatilla chinensis, Radish seeds, Malt, Atractylodes macrocephala, Dioscorea opposita, and Codonopsis pilosula, put them into a casserole, stir-fry over low heat for 15 to 30 minutes, add ethanol and water, and boil for 2 to 4 hours; S3: After decocting, the filtrate obtained by filtration is collected to prepare a Chinese medicine extract.

9. The Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to claim 1, characterized in that: The mass ratio of the traditional Chinese medicine extract to the polymerized combined accelerator is 8 to 12:

1.

10. The method for preparing the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: Place the Chinese medicine extract in a container, control the temperature at 60-70°C, and concentrate the Chinese medicine extract to a concentrated solution with a relative density of 1.2-1.5; Step 2: Add auxiliary materials and polymer joint accelerators to the concentrated solution in sequence, stir evenly, and obtain a Chinese medicine mixture; Step 3: prepare the Chinese medicine mixture with deionized water into oral liquids of different concentrations, and then pasteurize and fill them to obtain the Chinese medicine oral liquid for treating damp-heat diarrhea in pigs and chickens.

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