Preparation method of dendrobium polysaccharide with antioxidant and hypoglycemic activity

Abstract

The invention discloses a preparation method of dendrobium polysaccharide with antioxidant and hypoglycemic activity, which comprises the following steps: (1) cleaning fresh dendrobium candidum strips, cutting into sections, adding water, pulping into paste, filtering, and collecting filtrate to obtain dendrobium candidum homogenate; (2) treating the dendrobium officinale homogenate by using a pulsed electric field; (3) adding hot water into the dendrobium officinale homogenate treated in the step (2), and extracting polysaccharide; and (4) adding high-temperature-resistant alpha-amylase, saccharifying enzyme and protease into the extracting solution for enzymolysis, centrifuging after enzymolysis is finished, taking supernate, dialyzing, and freeze-drying to obtain the dendrobium officinale polysaccharide. According to the method disclosed by the invention, the fresh dendrobium officinale strips are used as raw materials, the dendrobium polysaccharides with antioxidant activity and activity of inhibiting alpha-amylase and advanced saccharification end products can be rapidly and efficiently separated and enriched, and the application of the product in end food is expanded.

Description

Technical Field

[0001] The invention relates to the field of medical technology, and in particular to a method for preparing a novel Dendrobium officinale polysaccharide having antioxidant properties and the ability to inhibit the activity of alpha-amylase and advanced saccharification end products (AGEs). Background Art

[0002] Antioxidants include natural antioxidants and synthetic antioxidants. Natural antioxidants such as red clover extract, vitamin C, E, etc. have received widespread attention due to their safety and high efficiency. Although synthetic antioxidants such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are widely used, they have potential health risks. Using natural antioxidants to replace synthetic antioxidants in the food industry has become a future development trend.

[0003] Diabetes is a global endocrine metabolic disease characterized by elevated blood sugar levels. According to data released by the International Diabetes Federation, the number of diabetes patients worldwide has reached hundreds of millions, and is becoming younger. The pathogenesis of diabetes is closely related to oxidative stress. Excessive reactive oxygen species produced by oxidative stress can directly damage pancreatic β cells, causing reduced insulin secretion or insulin resistance, leading to elevated blood sugar. Antioxidant therapy can reduce the damage of oxidative stress to pancreatic β cells, improve insulin sensitivity, and reduce insulin resistance, thereby helping to lower blood sugar levels. With the deepening of understanding of the pathogenesis of diabetes, the development of new, efficient and safe hypoglycemic drugs has become a research hotspot.

[0004] AGEs are the product of excess sugar and protein. There are two main sources of AGEs in the human body. One is produced through a series of reactions between excess sugar and protein, and the other is produced through the intake of AGEs contained in food. These AGEs are harmful to the human body and will bind to tissue cells and destroy cells. Studies have shown that AGEs are related to a series of chronic diseases such as diabetes, atherosclerosis and Alzheimer's disease, and are the culprit for accelerating human diseases and aging.

[0005] Dendrobium polysaccharide (DOP) is an important bioactive component in Dendrobium, with multiple biological activities such as anti-oxidation and hypoglycemic. However, the traditional extraction method of Dendrobium polysaccharide has problems such as low efficiency, low purity and complex operation, which limits its application in the medical field. Therefore, the development of an efficient, simple and high-purity extraction method of Dendrobium polysaccharide is of great significance for its application in the medical field. Summary of the invention

[0006] In view of the above problems existing in the prior art, the present invention provides a method for preparing dendrobium polysaccharides with antioxidant and hypoglycemic activities. The present invention uses fresh dendrobium officinale strips as raw materials, can quickly and efficiently separate and enrich dendrobium polysaccharides with antioxidant activity, α-amylase inhibition and advanced saccharification end products (AGEs) activity, and expand the application of this product in terminal foods.

[0007] The technical solution of the present invention is as follows:

[0008] The first object of the present invention is to provide a method for preparing Dendrobium officinale polysaccharide, the preparation method comprising the following steps:

[0009] (1) Cleaning and cutting fresh Dendrobium officinale strips, adding water, homogenizing into a paste, filtering, collecting the filtrate, and preparing a Dendrobium officinale homogenate;

[0010] (2) using pulsed electric field to treat the homogenate of Dendrobium officinale;

[0011] (3) adding hot water to the homogenate of Dendrobium officinale treated in step (2) to extract polysaccharides;

[0012] (4) adding a thermostable α-amylase, a saccharifying enzyme and a neutral protease to the extract in sequence for enzymolysis, centrifuging after the enzymolysis, taking the supernatant for dialysis and freeze-drying to obtain Dendrobium officinale polysaccharide (DOP).

[0013] In one embodiment of the present invention, in step (1), the length of the cut segments is 2-3 cm; the filtered residue is squeezed 2-3 times again, and the filtrate is combined.

[0014] In one embodiment of the present invention, in step (1), the homogenization time is 5-10 minutes.

[0015] In one embodiment of the present invention, in step (1), the volume ratio of the mass of fresh Dendrobium officinale strips to water is 1:5-10 g / mL.

[0016] In one embodiment of the present invention, in step (1), the filtration is directly performed by squeezing and filtering with gauze.

[0017] In one embodiment of the present invention, in step (2), the pulse electric field treatment conditions are: electric field frequency is 100-200 Hz, pulse width is 6-20 μs, and electric field strength is 5-15 kV / cm.

[0018] In one embodiment of the present invention, in step (3), the volume ratio of Dendrobium officinale homogenate to hot water is 1:2-5; the temperature of the hot water is 60°C; the extraction temperature is 95-100°C, and the extraction time is 2h.

[0019] In one embodiment of the present invention, in step (3), an enzyme reactor is used to precisely control the temperature for extraction.

[0020] In one embodiment of the present invention, in step (4), the amount of thermostable α-amylase used is 0.1-1wt% of the extract; the pH of the reaction system is 5.8-6.5, the temperature is 95-100°C, and the enzymolysis time is 2h.

[0021] In one embodiment of the present invention, the amount of saccharifying enzyme used is 0.1-1wt% of the extract; the pH value of the reaction system is 3-4.5, the temperature is 30-60°C, and the enzymolysis time is 2h.

[0022] In one embodiment of the present invention, in step (4), the amount of neutral protease used is 0.1-1 wt %; the pH value of the reaction system is 6.0-8.0, the temperature is 20-60° C., and the enzymolysis time is 2 h.

[0023] In one embodiment of the present invention, in step (4), enzymatic hydrolysis is performed step by step, and the enzyme does not need to be inactivated at the end of each step, but the pH value needs to be adjusted.

[0024] In one embodiment of the present invention, in step (4), the centrifugation condition is 8000 rpm for 15 min.

[0025] In one embodiment of the present invention, in step (4), the dialysis condition is dialysis in pure water, the dialysis bag cutoff is 7000Da, and the dialysis time is 72h.

[0026] The second object of the present invention is to provide a Dendrobium officinale polysaccharide prepared by the above preparation method.

[0027] The prepared Dendrobium officinale polysaccharide has a purity of 85-90wt%, can inhibit the activity of alpha-amylase, inhibit the generation of AGEs, and also has anti-DPPH activity.

[0028] The third object of the present invention is to provide an application of the above-mentioned Dendrobium officinale polysaccharide for preparing feed, medicine, food or health care products.

[0029] The beneficial technical effects of the present invention are:

[0030] The preparation method of the invention has high repeatability and can realize industrial production.

[0031] In the preparation process of the present invention, the Dendrobium officinale homogenate is treated by a pulse electric field, which destroys the structure of the cell membrane and causes perforation of the cell membrane, thereby accelerating the dissolution of the Dendrobium polysaccharide.

[0032] The dendrobium polysaccharide prepared by the invention has good antioxidant capacity and the ability to inhibit alpha amylase and AGEs, and is beneficial to the prevention of diabetes and human health. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The inhibitory activity of Dendrobium officinale polysaccharide on amylase obtained in Example 1.

[0034] Figure 2 The DPPH free radical scavenging activity of the Dendrobium officinale polysaccharide obtained in Example 1.

[0035] Figure 3 The anti-AGEs activity of the Dendrobium officinale polysaccharide prepared in Example 1.

[0036] Figure 4 This is the molecular weight analysis of the Dendrobium officinale polysaccharide obtained in Example 2. DETAILED DESCRIPTION

[0037] The present invention is described in detail below in conjunction with the accompanying drawings and embodiments.

[0038] Anti-α-amylase activity verification:

[0039] The sample was appropriately diluted to make its inhibition rate (AR, %) of α-amylase less than 50%. In the inhibition group, 0.25mL α-amylase solution (1.5U / mL) and 0.25mL polysaccharide sample were added to 0.5mL PBS, and after 10min in a 37℃ water bath, 0.5mL 1% (w / w) soluble starch solution was added. After accurate reaction for 5min, 1mL DNS reagent was added to terminate the reaction. The reaction solution was heated in a boiling water bath for 10min and then quickly placed in an ice water bath to cool to room temperature, and then 5mL deionized water was added. After mixing evenly, the absorbance value was measured at a wavelength of 540nm. During the measurement process, a blank group, a blank control group and an inhibition control group were set up. No sample was added to the blank group; no α-amylase solution and sample were added to the blank control group; no α-amylase solution was added to the inhibition control group. The insufficient volume was supplemented with PBS.

[0040] The activity (U) of α-amylase used in the assay was defined as the amount of α-amylase required to catalyze starch to produce 1 μmol of glucose within 1 min at 37°C / pH 6.9.

[0041] R=(1-(A3-A4) / (A1-A2))×100% (1)

[0042] Wherein, A1, A2, A3 and A4 are the absorbance values ​​of blank group, blank control group, inhibition group and inhibition control group at 540 nm, respectively.

[0043] Anti-DPPH activity verification:

[0044] The absorbance was measured at 517 nm. Dendrobium polysaccharide was dissolved in distilled water to prepare polysaccharide solutions of different concentrations of 0.1-5 mg / mL. DPPH solution (0.04 mg / mL) was prepared using anhydrous ethanol. Inhibition group: 1 mL of polysaccharide solution was added to 2 mL of DPPH solution; blank group: 1 mL of anhydrous ethanol was added to 2 mL of DPPH solution. The reaction was shaded at room temperature for 30 minutes, and the DPPH free radical scavenging rate was calculated according to formula (2):

[0045] DPPH free radical scavenging rate (%) = (A0-A1) / A0×100(2)

[0046] Among them, A0 is the absorbance of the blank group; A1 is the absorbance of the inhibition group.

[0047] Anti-AGEs activity assay:

[0048] The BSA-fructose model was used to determine the inhibitory effect of Dendrobium polysaccharides on the formation of AGEs. BSA (10 mg / mL) and fructose (0.25 M) were prepared using PBS (50 mM, pH 7.4). BSA and fructose were incubated at 50 °C for 24 h, and then the total AGEs of the samples were measured at an excitation wavelength of 370 nm and an emission wavelength of 440 nm. Inhibition group: 100 μL BSA, 100 μL fructose, 100 μL polysaccharide solution; blank group: 100 μL BSA, 100 μL fructose, 100 μL polysaccharide solution

[0049] Inhibition rate (%) = (1-A1 / A0) × 100 (3)

[0050] Among them, A0 is the absorbance of the blank group; A1 is the absorbance of the inhibition group.

[0051] Example 1

[0052] A method for preparing Dendrobium officinale polysaccharide comprises the following steps:

[0053] (1) 50 g of fresh Dendrobium officinale strips were cleaned, cut into 3 cm long segments, added with 400 mL of deionized water, homogenized with a juicer for 5 min, squeezed and filtered with gauze, and the residue was squeezed twice again. The filtrate was combined to prepare Dendrobium officinale homogenate.

[0054] (2) The Dendrobium officinale homogenate was treated with a pulsed electric field with an electric field frequency of 100 Hz, a pulse width of 10 μs, and an electric field strength of 5 kV / cm.

[0055] (3) Add 5 times the volume of 60°C deionized water to the Dendrobium officinale homogenate, use an enzyme reactor to precisely control the temperature at 98°C, and extract for 2 h.

[0056] (4) 0.5 wt% of thermostable α-amylase was added to the extract, and the pH value was 6.0 and the temperature was 98°C for 2 h. Then, 0.5 wt% of saccharifying enzyme was added to the system, and the pH value was 4.5 and the temperature was 58°C for 2 h. Then, 0.5 wt% of neutral protease was added to the system, and the pH value was 7.5 and the temperature was 45°C for 2 h.

[0057] (5) After the enzymatic hydrolysis, the enzymatic hydrolyzate was centrifuged at 8000 rpm for 15 min, the supernatant was dialyzed, the molecular weight cut-off of the dialysis bag was 7000 Da, and freeze-dried to obtain Dendrobium officinale polysaccharide. The extraction rate of Dendrobium officinale polysaccharide was 31.7%, and the purity was 88%.

[0058] Example 2

[0059] A method for preparing Dendrobium officinale polysaccharide comprises the following steps:

[0060] (1) 50 g of fresh Dendrobium officinale strips were cleaned, cut into 3 cm long segments, added with 500 mL of deionized water, homogenized with a juicer for 5 min, squeezed and filtered with gauze, and the residue was squeezed twice again. The filtrate was combined to prepare Dendrobium officinale homogenate.

[0061] (2) The Dendrobium officinale homogenate was treated with a pulsed electric field with an electric field frequency of 150 Hz, a pulse width of 15 μs, and an electric field strength of 8 kV / cm.

[0062] (3) Add 5 times the volume of 60°C deionized water to the Dendrobium officinale homogenate, use an enzyme reactor to precisely control the temperature at 95°C, and extract for 2 h.

[0063] (4) 0.5 wt% of thermostable α-amylase was added to the extract, and the pH value was 6.0 and the temperature was 98°C for 2 h. Then, 0.5 wt% of saccharifying enzyme was added to the system, and the pH value was 4.5 and the temperature was 58°C for 2 h. Then, 0.5 wt% of neutral protease was added to the system, and the pH value was 7.5 and the temperature was 45°C for 2 h.

[0064] (5) After the enzymatic hydrolysis, the enzymatic hydrolyzate was centrifuged at 8000 rpm for 15 min, the supernatant was dialyzed with a molecular weight cutoff of 7000 Da, and freeze-dried to obtain Dendrobium officinale polysaccharide. The extraction rate of Dendrobium officinale polysaccharide was 33.6% and the purity was 90%.

[0065] Example 3

[0066] A method for preparing Dendrobium officinale polysaccharide comprises the following steps:

[0067] (1) 50 g of fresh Dendrobium officinale strips were cleaned, cut into 3 cm long segments, added with 500 mL of deionized water, homogenized with a juicer for 5 min, squeezed and filtered with gauze, and the residue was squeezed twice again. The filtrate was combined to prepare Dendrobium officinale homogenate.

[0068] (2) The Dendrobium officinale homogenate was treated with a pulsed electric field with an electric field frequency of 200 Hz, a pulse width of 20 μs, and an electric field strength of 10 kV / cm.

[0069] (3) Add 4 times the volume of 60°C deionized water to the Dendrobium officinale homogenate, use an enzyme reactor to precisely control the temperature at 100°C, and extract for 2 h.

[0070] (4) 0.2 wt% of thermostable α-amylase was added to the extract, and the pH value was 6.0 and the temperature was 95°C for 2 h. Then, 0.5 wt% of saccharifying enzyme was added to the system, and the pH value was 4 and the temperature was 60°C for 2 h. Then, 0.8 wt% of neutral protease was added to the system, and the pH value was 6.0 and the temperature was 50°C for 2 h.

[0071] (5) After the enzymatic hydrolysis, the enzymatic hydrolyzate was centrifuged at 8000 rpm for 15 min, and the supernatant was dialyzed with a molecular weight cutoff of 7000 Da, and freeze-dried to obtain Dendrobium officinale polysaccharide. The extraction rate of Dendrobium officinale polysaccharide was 33.8% and the purity was 90.7%.

[0072] Comparative Example 1

[0073] A method for preparing Dendrobium officinale polysaccharide comprises the following steps:

[0074] (1) 50 g of fresh Dendrobium officinale strips were cleaned, cut into 3 cm long segments, added with 400 mL of deionized water, homogenized with a juicer for 5 min, squeezed and filtered with gauze, and the residue was squeezed twice again. The filtrate was combined to prepare Dendrobium officinale homogenate.

[0075] (2) Add 5 times the volume of 60°C deionized water to the homogenate of Dendrobium officinale, use an enzyme reactor to precisely control the temperature at 98°C, and extract for 2 h.

[0076] (3) 0.5 wt% of thermostable α-amylase was added to the extract, and the pH value was 6.0 and the temperature was 98°C for 2 h. Then, 0.5 wt% of saccharifying enzyme was added to the system, and the pH value was 4.5 and the temperature was 58°C for 2 h. Then, 0.5 wt% of neutral protease was added to the system, and the pH value was 7.5 and the temperature was 45°C for 2 h.

[0077] (4) After the enzymatic hydrolysis, the enzymatic hydrolyzate was centrifuged at 8000 rpm for 15 min, the supernatant was dialyzed, the molecular weight cut-off of the dialysis bag was 7000 Da, and freeze-dried to obtain Dendrobium officinale polysaccharide. The extraction rate of Dendrobium officinale polysaccharide was 27.4%, and the purity was 87%.

[0078] Comparative Example 2

[0079] A method for preparing Dendrobium officinale polysaccharide comprises the following steps:

[0080] (1) 50 g of fresh Dendrobium officinale strips were cleaned, cut into 3 cm long segments, added with 500 mL of deionized water, homogenized with a juicer for 5 min, squeezed and filtered with gauze, and the residue was squeezed twice again. The filtrate was combined to prepare Dendrobium officinale homogenate.

[0081] (2) The pulsed electric field was used to treat the homogenate of Dendrobium officinale, with an electric field frequency of 150 Hz, a pulse width of 15 μs, and an electric field strength of 8 kV / cm

[0082] (3) Add 5 times the volume of 60℃ deionized water to the Dendrobium homogenate, the extraction temperature is 95℃, and the extraction time is 2h

[0083] (4) Carry out the enzymatic hydrolysis with saccharifying enzyme and neutral protease in steps, and inactivate the enzymes at the end of the experiment. The addition amount of the two enzymes is 0.5 wt %, the enzymatic hydrolysis temperature is 58° C. and 45° C., the solution pH value is 4.5 and 7.5, and the enzymatic hydrolysis time is 2 h.

[0084] (5) After the enzymatic hydrolysis, the enzymatic hydrolyzate was centrifuged at 8000 rpm for 15 min, the supernatant was dialyzed, the molecular weight cut-off of the dialysis bag was 7000 Da, and freeze-dried to obtain Dendrobium officinale polysaccharide. The extraction rate of Dendrobium officinale polysaccharide was 32.9%, and the purity was 78%.

[0085] Test example:

[0086] (1) α-amylase, DPPH inhibitory activity and anti-AGEs activity

[0087] The dendrobium polysaccharide obtained in Example 1 was tested for α-amylase inhibition activity. The results are as follows: Figure 1 As shown, the inhibitory activity of Dendrobium polysaccharide on α-amylase increases with the increase of concentration. At 9 mg / mL, the inhibition rate is about 36%, which has good α-amylase inhibitory activity.

[0088] The polysaccharide obtained in Example 1 was subjected to DPPH inhibition activity assay. Figure 2As shown in the figure, the inhibitory activity of dendrobium polysaccharide on DPPH increases with the increase of concentration, and the inhibition rate is about 36% at 4 mg / mL, which has good DPPH inhibitory activity. The free radical inhibition experiment proves that dendrobium polysaccharide has excellent antioxidant capacity.

[0089] The anti-AGEs activity of the dendrobium polysaccharide obtained in Example 1 was identified. Figure 3 As shown, the inhibitory ability of Dendrobium polysaccharide on AGEs is concentration-dependent. At a concentration of 1 mg / mL, the inhibition rate is 70%. With the increase of the concentration of Dendrobium polysaccharide, the final inhibition rate reaches 89%, which has good AGEs inhibitory activity.

[0090] (2) Molecular weight and composition analysis of Dendrobium polysaccharides

[0091] The relative molecular weight distribution of the Dendrobium polysaccharide prepared in Example 2 was determined by high performance liquid gel filtration chromatography. The chromatographic conditions are as follows: the mobile phase is a 0.1 mol / L NaNO3 solution with 0.02% NaN3 added; the flow rate is 0.5 mL / min; the high performance liquid chromatograph is equipped with a 2414 differential detector, a Waters e2695 operating system, and Empower data processing software. The column temperature and the differential temperature are both 45°C; the injection volume is 15 μL; the data acquisition time is 30 min. A series of pullulan polysaccharides and glucose with known molecular weights of 0.18 kDa, 2.7 kDa, 9.75 kDa, 135.35 kDa, and 300.60 kDa are used as standards. The sample concentration is 10 mg / mL, and it is centrifuged at high speed (8000 rpm, 15 min) and filtered through a 0.22 μm filter membrane for analysis and determination to obtain data such as the relative weight average molecular weight (R-Mw) of the Dendrobium polysaccharide. The results are as follows Figure 4 In Example 2, the peak molecular weight of the prepared Dendrobium polysaccharide is 412864Da, and the relative molecular weight is 492480Da.

[0092] The dendrobium polysaccharide prepared in Example 1 was weighed to 10 mg (accurate to 0.1 mg), and the polysaccharide sample was dissolved in 4M trifluoroacetic acid (TFA) and acid-lyzed at 121°C for 2h. The acid solution was dissolved in distilled water after being dried by nitrogen, filtered with a 0.45μm filter membrane, and used for the determination of ion exchange chromatography system. 250mM NaOH and 1M NaAc were used as eluents, and the column was passed at a flow rate of 0.5mL / min at 30°C. Standards include fucose, rhamnose, arabinose, galactose, glucose, xylose, mannose, fructose, glucuronic acid and galacturonic acid. The monosaccharide composition was analyzed by ICS-5000 ion chromatography system. As shown in Table 1, the dendrobium polysaccharide is mainly composed of two monosaccharides, mannose and glucose, with a molar ratio of 5.9:1.

[0093] Table 1

[0094] Monosaccharides Proportion(%) Mannose 84.2％ glucose 14.3％

[0095] The embodiments provided above are not intended to limit the scope of the present invention, and the steps described are not intended to limit the execution order thereof. Those skilled in the art may make obvious improvements to the present invention in combination with existing common knowledge, which also fall within the scope of protection defined by the claims of the present invention.

Claims

1. A method for preparing Dendrobium officinale polysaccharide, characterized in that: The preparation method comprises the following steps: (1) Cleaning and cutting fresh Dendrobium officinale strips, adding water, homogenizing into a paste, filtering, collecting the filtrate, and preparing a Dendrobium officinale homogenate; (2) using pulsed electric field to treat the homogenate of Dendrobium officinale; (3) adding hot water to the homogenate of Dendrobium officinale treated in step (2) to extract polysaccharides; (4) adding a thermostable α-amylase, a saccharifying enzyme and a neutral protease to the extract in sequence for enzymolysis, centrifuging after the enzymolysis, taking the supernatant for dialysis and freeze-drying to obtain a Dendrobium officinale polysaccharide.

2. The preparation method according to claim 1, characterized in that: In step (1), the length of the cut segments is 2-3 cm; the filtered residue is squeezed 2-3 times again, and the filtrate is combined.

3. The preparation method according to claim 1, characterized in that: In step (2), the pulse electric field treatment conditions are: electric field frequency is 100-200 Hz, pulse width is 6-20 μs, and electric field strength is 5-15 kV / cm.

4. The preparation method according to claim 1, characterized in that: In step (3), the volume ratio of Dendrobium officinale homogenate to hot water is 1:2-5; the temperature of the hot water is 60° C.; the extraction temperature is 95-100° C., and the extraction time is 2 h.

5. The preparation method according to claim 1, characterized in that: In step (4), the amount of thermostable α-amylase used is 0.1-1wt% of the extract; the pH of the reaction system is 5.8-6.5, the temperature is 95-100°C, and the enzymolysis time is 2h.

6. The preparation method according to claim 1, characterized in that: In step (4), the amount of saccharifying enzyme used is 0.1-1wt% of the extract; the pH value of the reaction system is 3-4.5, the temperature is 30-60°C, and the enzymatic hydrolysis time is 2h.

7. The preparation method according to claim 1, characterized in that: In step (4), the amount of neutral protease used is 0.1-1wt%; the pH value of the reaction system is 6.0-8.0, the temperature is 20-60°C, and the enzymolysis time is 2h.

8. The preparation method according to claim 1, characterized in that: In step (4), the enzymatic hydrolysis is carried out step by step. It is not necessary to inactivate the enzyme at the end of each step, but the pH value needs to be adjusted.

9. A Dendrobium officinale polysaccharide prepared by the preparation method according to any one of claims 1 to 8.

10. An application of the Dendrobium officinale polysaccharide according to claim 9, characterized in that: Used for preparing feed, medicine, food or health products.

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