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Method for purifying cephalosporin C broth using adsorbent

A technology of broth and aminocephalosporanic acid, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc.

Inactive Publication Date: 2006-08-30
CHONGKUNDANG BIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the case of the enzymatic method, since deacetylated CPC exists directly in 7-ACA in the form of deacetylated 7-ACA, a process of removing deacetylated CPC from the CPC broth is necessary

Method used

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  • Method for purifying cephalosporin C broth using adsorbent
  • Method for purifying cephalosporin C broth using adsorbent
  • Method for purifying cephalosporin C broth using adsorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Using SEPABEADS  Adsorption and Elution of Cephalosporin C Broth Filtrate by SP-700 Resin

[0035] The CPC broth filtrate (deacetylated CPC content: 6%) was adsorbed at a rate of 3BVH on a bed filled with SEPABEADS  A column of SP-700 resin (diameter: 6 cm, height: 40 cm, column bed volume: 3000 ml) was used and eluted. After adsorption and elution, the distribution of CPC and deacetylated CPC as figure 2 shown.

[0036] exist figure 2 When the eluate was collected above the 6BV point, the deacetylated CPC content in the CPC solution was 0.9%, while the deacetylated CPC content at the 6BV point was 3.5%.

Embodiment 2

[0037] Example 2: Using SEPABEADS  Adsorption and Elution of Cephalosporin C Broth Filtrate by SP-825 Resin

[0038] In addition to using SEPABEADS  SP-825 resin instead of SEPABEADS  Except for SP-700 resin, the CPC broth filtrate was adsorbed and eluted according to the same method as in Example 1. result in image 3given in.

Embodiment 3

[0039] Example 3: Using SEPABEADS  Adsorption and Elution of Cephalosporin C Broth Filtrate by SP-850 Resin

[0040] In addition to using SEPABEADS  SP-850 resin instead of SEPABEADS  Except for SP-700 resin, the CPC broth filtrate was adsorbed and eluted according to the same method as in Example 1. result in Figure 4 given in.

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Abstract

A method for purification of cephalosporin C broth using absorbent is provided, thereby effectively removing diacetyl CPC(cephalosporin C) from the cultured medium. The method for purification of cephalosporin C broth using absorbent comprises the steps of: filtering and absorbing cephalosporin C(CPC) cultured medium using absorbent such as polystyrene resin, and eluting it to remove diacetyl CPC from the CPC cultured medium; reacting cephalosporin C(CPC) cultured medium with D-amino acid oxidase(DAOD) to prepare glutaryl-7-aminocephalosporanic acid(Gl-7-ACA); and reacting glutaryl-7-aminocephalosporanic acid(Gl-7-ACA) with glutaryl-7-aminocephalosporanic acid acylase(Gl-7-ACA acylase) to prepare 7-aminocephalosporanic acid(7-ACA), wherein the CPC cultured medium has pH 5.0 to 6.0; and the eluting solution is phosphate buffer solution with pH 6 to 9, acetate buffer solution, carbonate buffer solution or weak alkali solution.

Description

technical field [0001] The present invention relates to a process for preparing 7-aminocephalosporanic acid (7-ACA) by enzymatically purifying cephalosporin C (CPC) broth. More specifically, the present invention relates to a method for separating and removing deacetylated cephalosporin C (deacetylated CPC) from CPC broth by using an adsorbent. Background technique [0002] Methods for preparing 7-ACA from CPC are generally divided into chemical methods and enzymatic methods. [0003] According to chemical methods, crystals of CPC salt are obtained from CPC broth and hydrolyzed to synthesize 7-ACA (Belgian Patent No. 615955). Chemical methods are deficient due to the use of highly toxic contaminating reactants (eg, chlorinated solvents, chlorosilanes, dimethylaniline, etc.) and the need for severe operating conditions (extremely low temperatures, eg -50°C). [0004] According to the enzymatic method, after the CPC broth was purified, the purified filtrate was subjected to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P35/04C12P35/00
CPCC12N9/80C12P35/06C12Y305/01093
Inventor 金正优申相圭尹钟赞尹仁均
Owner CHONGKUNDANG BIO