Homogenising of small-volume mixtures by centrifugation and heating

A technology of action and centrifugal force, applied in chemical reaction in liquid medium, in the field of chemical reaction, can solve the problem that the equipment does not meet the needs of fast and accurate thermal cycle equipment and methods

Inactive Publication Date: 2002-06-05
阿尔法赫里克斯有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] In particular, the devices and methods of the prior art do not meet the need for rapid and accurate thermal cycling devices and methods

Method used

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  • Homogenising of small-volume mixtures by centrifugation and heating
  • Homogenising of small-volume mixtures by centrifugation and heating
  • Homogenising of small-volume mixtures by centrifugation and heating

Examples

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no. 1 example

[0025] figure 1 is a schematic representation of a first embodiment of the invention having a temperature measuring device (7) connected to a processor (4);

[0026] figure 2 represents a second embodiment of the invention, wherein the temperature measuring device (7) is supplemented by a laser source (8) and a sensor (9);

[0027] image 3 represents a third embodiment of the invention, wherein the temperature is measured by a probe (12) connected to the processor (4) via a transmitter (13) and receiver (14);

[0028] Figure 4 shows a schematic cross-sectional view of the apparatus according to the invention, showing essential components, such as the heating mantle (6);

[0029] Figure 5 means from figure 2 The upper part of the shown embodiment sees the resulting schematic diagram;

[0030] Figure 6 means from e.g. figure 1 The upper portion of the illustrated embodiment sees the resulting schematic diagram;

[0031] Figure 7 It shows a schematic cross-secti...

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PUM

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Abstract

The efficient temperation of a sample is achieved when the sample is subjected to centrifugation simultaneously wish heating and optionally also cooling. This is achieved in a device comprising at least a rotor (1) for holding reaction vessels (2), a motor (3), connected to the rotor, a processor (4) for controlling the speed of the rotor, and elements (5 and 6) for heating and cooling the contents of the reaction vessels, wherein the elements (6) for heating cover the apices of the reaction vessels for at least part of the rotational path of the vessels and that the elements for heating operate at a temperature significantly higher than the meeting temperature of the reaction vessels. The invention further relates to a method for performing chemical reactions and in particular a method for performing biochemical reactions involving thermocycling, for example PCR-reactions.

Description

[0001] technical field of invention [0002] The invention relates to the field of chemical reactions requiring uniform mixing and / or heat preservation of reactants, in particular to the field of chemical reactions in liquid media. The present invention discloses a new equipment and method for effectively and uniformly mixing, smoothing and / or insulated reactants. background of the invention [0003] Many important industrial processes and processes used in various laboratories rely on chemical reactions. The time it takes to complete a process or process is usually determined by the time it takes for a particular chemical reaction or reactions to reach chemical equilibrium. This is often referred to as the dynamic nature of a chemical reaction, or simply reaction kinetics. In each case, there are many variables that affect the kinetics of this reaction, for example, concentrations of reactants, temperature, presence or absence of a catalyst, etc. [0004] In general, incre...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N15/09B01F3/08B01F9/00B01J19/00B01J19/18B01L7/00B04B5/04B04B15/02C07B61/00G01N1/44G01N35/00
CPCG01N1/44B01L7/52B01L7/5255B04B5/0407B01F9/0003B04B15/02G01N2035/00495Y10T436/25375B04B5/0414B04B5/0421B01F29/15
InventorM·马姆奎斯特
Owner阿尔法赫里克斯有限公司