Biological detection system

Abstract

The biological detection system comprises a biological sample processing device which is used for providing a fully-closed place for nucleic acid extraction and amplification detection of a sample to be detected, the biological sample processing device is provided with a plurality of chambers and at least comprises a sample cracking chamber, a cleaning chamber, an amplification detection chamber and fluid channels communicated with the plurality of chambers, and a plurality of valves are arranged among the fluid channels; the valve lock device comprises an actuating mechanism and a plurality of ejection mechanisms which are actuated by the actuating mechanism and are removably jointed with the valves, and is used for controlling the opening and closing of each valve through the actuating mechanism and the ejection mechanisms according to different treatment processes of the sample to be detected; the temperature control device is used for providing the temperature required in the nucleic acid elution and amplification detection process; the optical detection device is used for performing fluorescence detection on the to-be-detected sample; the valve lock device comprises an actuating motor, and a plurality of ejection mechanisms are distributed along a straight line. The opening and closing of each valve of the biological sample treatment device are controlled by one actuating motor, so that the production cost is reduced, and the ejection mechanism is linearly distributed, so that the miniaturization of the instrument is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of biological sample detection, in particular to a biological detection system. Background Art

[0002] Nucleic acid testing has been widely used in many fields such as disease diagnosis and prognosis, public health, food safety, molecular breeding, forensic identification, etc. Nucleic acid testing mainly includes: sample processing, nucleic acid extraction and purification, nucleic acid amplification and detection. Traditional nucleic acid testing in laboratories requires specialized operators and physically partitioned spaces, and many steps require manual operation. Not only is the operation cumbersome, but there is also the risk of samples and reagents easily causing harm to operators and nucleic acid product contamination.

[0003] The fully integrated and automated nucleic acid detection system can solve the above problems well and achieve the detection goal of "sample in - result out". It not only greatly reduces human operation and reduces the potential contamination hazards of samples and amplification products, but also improves the reliability of detection, saves labor costs and reduces space occupancy to the greatest extent.

[0004] Portable fully integrated nucleic acid testing, also known as "molecular diagnostic POCT (point-of-care testing)", is a portable, miniaturized, automated nucleic acid detection system that uses external automated equipment to simulate manual operations to achieve a fully automated process from sample processing to nucleic acid testing.

[0005] The detection consumables box of conventional molecular POCT products integrates the functions of nucleic acid extraction and nucleic acid amplification, and cooperates with the instruments of molecular POCT products to realize the whole process from sample processing to nucleic acid detection. The mainstream technology of molecular diagnosis is fluorescent quantitative PCR technology. Due to the characteristics of exponential amplification templates in PCR technology, the existing molecular POCT from sample processing to nucleic acid detection must be fully automatic and fully enclosed in the whole process, so as to avoid aerosol contamination to the greatest extent in the absence of PCR partitioned laboratories. This not only puts high demands on the design of consumables boxes, but also on supporting instruments. While ensuring basic fully automatic and fully enclosed conditions, it is also necessary to consider how to reduce costs and reduce space occupancy. Utility Model Content

[0006] The utility model provides a biological detection system, which can reduce space occupation and save costs.

[0007] The biological detection system of the utility model comprises:

[0008] A biological sample processing device, used to provide a fully enclosed place for nucleic acid extraction and amplification detection of a sample to be detected, which has multiple chambers, the multiple chambers at least including a lysis chamber, a cleaning chamber and an amplification detection chamber, and a fluid channel connecting the multiple chambers, and multiple valves are arranged between the fluid channels;

[0009] A valve lock device, comprising an actuating mechanism and a plurality of ejection mechanisms actuated by the actuating mechanism and removably engaged with the valve, wherein the valve lock device is used to control the opening and closing of each valve in the biological sample processing device through the actuating mechanism and the ejection mechanism according to different processing processes of the sample to be detected, so as to control the flow or blocking of the sample to be detected between the plurality of chambers;

[0010] A temperature control device, used to provide the temperature required during nucleic acid elution and amplification detection;

[0011] An optical detection device, used for performing fluorescence detection on a sample to be detected;

[0012] Wherein, the actuating mechanism includes an actuating motor and a plurality of ejecting mechanisms are distributed along a straight line.

[0013] In one embodiment, each ejection mechanism corresponds to a valve of the biological sample processing device and at least one workstation, the workstation corresponds to a corresponding process in sample processing, and the ejection mechanism of the workstation simultaneously opens or closes the corresponding valve.

[0014] In one embodiment, the ejection mechanism is configured such that when the actuating mechanism is actuated once, the ejection mechanism undergoes a total of M working stations as the actuating mechanism is actuated, and the M working stations are less than the N steps required in the sample processing flow of biological detection.

[0015] In one embodiment, the M working stations include 5 stations, the N steps include at least 6 steps, and the steps at least include: sample lysis, binding of the lysed solution with magnetic particles, magnetic particle cleaning, elution, transfer of the nucleic acid to be tested, and PCR reaction.

[0016] In one embodiment, the actuating mechanism further includes a core shaft connected to the actuating electric transmission, the ejection mechanism is arranged corresponding to the valve and fixed on the core shaft, and is configured to rotate one circle with the core shaft, and the ejection mechanism at the corresponding workstation controls the opening or closing of the valve.

[0017] In one embodiment, the ejection mechanism includes a cam arranged corresponding to the valve and a rod assembly arranged corresponding to the cam and perpendicular to the core shaft, the cam is fixed on the core shaft and rotates with the core shaft, and the rod assembly is configured so that when the cam rotates to a corresponding angle with the core shaft, the rod assembly at the corresponding workstation is ejected by the raised portion of the cam to engage with the corresponding valve.

[0018] In one embodiment, the rod assembly includes a push rod having one end in contact with a raised portion of the cam, and a push rod sleeved on the other end of the push rod to engage with the valve, and the end of the push rod in contact with the raised portion of the cam is formed as a boss having a diameter greater than a diameter of the push rod body.

[0019] In one embodiment, the valve lock device also includes a valve lock core fixing part, the core shaft passes through the valve lock core fixing part and is rotatably connected to the valve lock core fixing part through a first bearing, wherein the valve lock core fixing part is also provided with grooves corresponding to the rod assemblies one by one, a spring is provided in the groove, a through hole is provided at the bottom of the groove for the rod assembly to pass through, the push rod passes through the spring and the through hole, one end of the spring abuts against the boss of the push rod, and the other end abuts against the bottom of the groove.

[0020] In one embodiment, the actuating motor is fixed below the valve lock core fixing member via a motor fixing member, the motor fixing member is fixed to one side of the valve lock core fixing member, one end of the core shaft passes through the motor fixing member, and is rotatably connected to the motor fixing member via a second bearing.

[0021] In one embodiment, the valve locking device also includes a code disk connected to the core shaft and a photoelectric component cooperating with the code disk. The code disk is fixed at the end of the core shaft opposite to the motor fixing part. The closed-loop control of the actuating motor is achieved through the cooperation between the code disk and the photoelectric component to detect the rotation angle of the core shaft and the cam.

[0022] In one embodiment, the valve locking device further comprises a transmission assembly fixed on the motor fixing member, and the core shaft is drivingly connected to the actuating motor via the transmission assembly.

[0023] Wherein, the transmission assembly comprises a driving wheel connected to the actuating motor and a driven wheel connected to the core shaft respectively, and the driving wheel and the driven wheel are connected by a synchronous belt.

[0024] In one embodiment, the push rod is made of soft material.

[0025] In one embodiment, the biological detection system further includes a biological sample processing device control component to apply a pressure difference between each chamber and the fluid channel of the biological sample processing device.

[0026] In one embodiment, each chamber of the biological sample processing device is respectively provided with a plunger for sealing each chamber, and the biological sample processing device control component includes a plunger push rod assembly, which is arranged corresponding to the plunger of each chamber of the biological sample processing device and is configured to push / pull the corresponding plunger in the corresponding sample processing process.

[0027] In one embodiment, the biological detection system further comprises an ultrasonic device, which abuts against the bottom of the sample lysis chamber and is used to emit ultrasonic waves to lyse the sample to be detected in the biological sample processing device.

[0028] In one embodiment, the biological sample processing device further comprises a magnetic particle binding chamber for storing magnetic particles and providing a place for the sample to be detected to bind to the magnetic particles.

[0029] In one embodiment, the biological detection system further comprises a magnetic attraction component for providing an external magnetic field to attract magnetic particles in the biological sample processing device.

[0030] Compared with the prior art, the biological detection system of the utility model can control the opening and closing of each valve of the biological sample processing device by only one actuating motor according to the different processing processes of the sample to be detected, thereby saving production costs. In addition, the utility model has multiple ejection mechanisms corresponding to the multiple valves of the biological sample processing device one by one. The multiple ejection mechanisms are distributed along a straight line, and the structural layout is simple, which reduces the volume space occupied by the device for controlling the opening and closing of each valve of the biological sample processing device, and contributes to the miniaturization of the instrument.

[0031] The above-mentioned technical features can be combined in various technically feasible ways to produce new implementation schemes, as long as the purpose of the present utility model can be achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The present invention will be described in more detail below based on non-limiting embodiments and with reference to the accompanying drawings, wherein:

[0033] Figure 1 The schematic diagram of the overall structure of the biological detection system according to the utility model is shown;

[0034] Figure 2-3 Shows Figure 1 A schematic diagram of the structure of the valve lock device of the biological detection system;

[0035] Figure 4 An exploded view of the valve lock assembly is shown;

[0036] Figure 5 A cross-sectional view of the valve lock assembly is shown;

[0037] Figure 6 The diagram showing the correspondence between the cam and the working position when the valve locking device is in operation is shown;

[0038] Figure 7 A schematic diagram of the structure of a biological sample processing device is shown;

[0039] Figure 8 A schematic structural diagram of a valve and a flow channel at the valve of a biological sample processing device is shown.

[0040] In the figures, the same components are indicated by the same reference numerals. The figures are not drawn to scale.

[0041] Wherein, the accompanying drawings are marked as follows:

[0042] 1. Biological sample processing device; 11. Lysis chamber; 12. Transition chamber; 13. Binding liquid chamber; 14. Cleaning liquid chamber; 15. Eluent chamber; 16. Magnetic chamber; 17. Reaction chamber; 18. Waste liquid chamber; 191. First valve; 192. Second valve; 193. Third valve; 194. Fourth valve; 195. Fifth valve; 196. Sixth valve; 197. First flow channel; 198. Second flow channel; 199. Third flow channel; 200. Diaphragm; 20. Plunger; 2. Valve lock device; 21. Transmission assembly; 211. Driving wheel; 212. Same Step belt; 213, driven wheel; 22, ejection mechanism; 221, push rod; 222, ejector rod; 223, spring; 23, actuating motor; 231, motor fixing member; 24, photoelectric component; 25, code disk; 26, valve lock core fixing member; 261, groove; 27, core shaft; 28, first cam; 29, second cam; 30, third cam; 31, fourth cam; 32, fifth cam; 33, stopper; 34, first bearing; 35, second bearing; 3, moving device; 4, ultrasonic device; 5, biological sample processing device control component. DETAILED DESCRIPTION

[0043] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, as long as there is no conflict, the various embodiments and features in the various embodiments of the present invention can be combined with each other, and the resulting technical solutions are all within the protection scope of the present invention.

[0044] The parts not described in the present invention can be realized by adopting or drawing on the existing technology.

[0045] Unless otherwise defined, the technical or scientific terms used in the present invention shall have the usual meanings understood by persons with ordinary skills in the field to which the present invention belongs. The terms "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

[0046] In the description of the present invention, the orientation or position relationship indicated by the term "vertical" etc. is based on the orientation or position relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. When the absolute position of the object being described changes, the relative position relationship may also change accordingly, and therefore cannot be understood as a limitation on the present invention.

[0047] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The embodiments will be described in detail below in conjunction with the accompanying drawings.

[0048] The notable features of molecular diagnostic POCT are that the instrument is small (mostly desktop type) and the nucleic acid extraction and amplification are fully automatic and fully enclosed, so it does not rely on the PCR laboratory.

[0049] Molecular diagnostic POCT instruments need to cooperate with fully enclosed biological sample processing devices to complete nucleic acid extraction and amplification testing.

[0050] To this end, the utility model proposes a biological detection system, which can reduce space occupation and save costs.

[0051] like Figure 1 As shown, the biological detection system of the utility model includes a biological sample processing device 1, which is used to provide a fully enclosed place for nucleic acid extraction and amplification detection of the sample to be detected. The device has multiple chambers, which include at least a sample lysis chamber, a cleaning chamber, an amplification detection chamber, and fluid channels connecting the corresponding chambers, and multiple valves for controlling the passage of fluids are arranged between the fluid channels.

[0052] The valve locking device 2 comprises an actuating mechanism and a plurality of ejection mechanisms actuated by the actuating mechanism and removably engaged with the valve. The valve locking device is used to control the opening and closing of each valve in the biological sample processing device through the actuating mechanism and the ejection mechanism according to different processing processes of the sample to be detected, so as to control the flow or blocking of the sample to be detected between the plurality of chambers.

[0053] A moving device 3, used for controlling the biological sample processing device 1 to move between different processing stations;

[0054] A temperature control device, used to provide the temperature required during nucleic acid elution and amplification detection;

[0055] An optical detection device, used for performing fluorescence detection on the sample to be tested;

[0056] The actuating mechanism includes an actuating motor 23, and a plurality of ejecting mechanisms 22 are distributed along a straight line.

[0057] In the biological detection system of the utility model, the opening and closing of each valve of the biological sample processing device can be controlled by only one actuating motor through the valve locking device according to the different processing processes of the sample to be detected. The control method is simple and the production cost is saved. In addition, there are multiple ejection mechanisms corresponding to the multiple valves of the biological sample processing device one by one. The multiple ejection mechanisms are distributed along a straight line, and the structural layout is simple, which reduces the volume space occupied by the device for controlling the opening and closing of each valve of the biological sample processing device, and helps to miniaturize the instrument.

[0058] In some optional embodiments, each ejection mechanism 22 corresponds to a valve of the biological sample processing device and at least one work station, and the work station corresponds to different combinations of the ejection mechanisms to simultaneously open or close the valves, and the ejection mechanism is configured to be separable and combinable with each valve in the biological sample processing device.

[0059] In some specific embodiments, the lysis chamber is used to lyse the sample to be processed entering therein to release the target substance in the sample to be processed. In an embodiment, the target substance is nucleic acid, and the function of the lysis chamber is to destroy the sample and release the nucleic acid in the sample into the lysis chamber.

[0060] The release of the target substance can be carried out in a physical release manner. For example, in some embodiments of the present application, a certain number of pyrolysis particles (such as polystyrene particles, borosilicate glass particles, quartz glass particles, etc.) can be pre-packaged in the pyrolysis chamber. When the sample to be processed enters the pyrolysis chamber, the target substance is released by processing the sample to be processed. For example, in some optional embodiments, the biological detection system may include an ultrasonic device 4, which emits ultrasonic waves to drive the pyrolysis particles to move in the pyrolysis chamber, grind the sample and release the target substance.

[0061] The moving device 3 can be a transmission belt device, a moving guide rail or a manipulator device, which is used to move the biological sample processing device 1 between different processing stations of the instrument. The different processing stations include but are not limited to loading stations, lysis stations, injection stations, magnetic stations, heating stations, and detection stations. The stations can be in the same or different positions, and can be the biological sample processing device moved to the corresponding processing component, or the corresponding processing component moved to the biological sample processing device.

[0062] In some optional embodiments, the user places the biological sample processing device at the loading station, uses ultrasound to lyse the sample to be tested at the lysis station, uses the plunger push rod assembly to push / pull the plungers of different chambers at the injection station, uses the magnetic suction assembly to adsorb magnetic particles at the magnetic suction station, controls the reaction temperature with a temperature control device and performs elution and heating at the heating station, and performs nucleic acid amplification detection at the detection station.

[0063] In some optional embodiments, the ejection mechanism 22 is configured to be actuated once by the actuating mechanism, and the ejection mechanism goes through M working stations with the actuation of the actuating mechanism, and the M working stations are less than the N steps required by the sample processing process of biological detection.

[0064] Among them, the workstation refers to the workstation of the ejection mechanism, each workstation corresponds to the corresponding process in the sample processing, the ejection mechanism of the workstation opens or closes the corresponding valve at the same time, and M workstations include 5 workstations. N steps include at least 6 steps, and the steps at least include: sample lysis, combination of the lysed solution and magnetic particles, magnetic particle cleaning, elution, transfer of nucleic acid to be tested, and PCR reaction.

[0065] like Figure 6 As shown, the five working positions, namely the first position, the second position, the third position, the fourth position and the fifth position, respectively represent the five working states of the valve lock device. When the actuating motor is actuated, the ejection mechanism 22 is driven by the actuating mechanism to eject in a preset logical sequence. At the corresponding working position, the ejection mechanism is combined or separated with the valve at the corresponding position to achieve the disconnection or connection between the flow channels, thereby controlling the flow of the corresponding fluid in the corresponding chamber and the flow channel.

[0066] like Figure 7 and Figure 8 As shown, Figure 7 A specific embodiment of the biological sample processing device is shown. In this embodiment, the biological sample processing device is vertically inserted into the detection instrument for use, so a membrane 200 is sealed on the surface of the biological sample processing device 1. Among them, the valves are all annular valves, and each flow channel is connected to the annular part of the valve. Figure 8The structure diagram of the valve and the flow channel at the valve of the biological sample processing device is schematically shown from the side of the biological sample processing device. Figure 8 The direction of the middle arrow indicates the moving direction of the ejection mechanism. The first flow channel 197 is connected to the chamber of the biological sample processing device 1 and the second flow channel 198 respectively. The second flow channel 198 is connected to the third flow channel 199, wherein the third flow channel 199 represents the annular flow channel of the valve.

[0067] When the ejection mechanism 22 is ejected and contacts the valve of the biological sample processing device 1, the ejection mechanism 22 presses the diaphragm 200 attached to the valve surface under the action of external force, thereby blocking the third flow channel 199 and blocking the connection between the second flow channel 198 and the third flow channel 199. The solution in the room is blocked in the room and will not flow into the third flow channel 199; when the ejection mechanism 22 is away from the valve, the connection between the second flow channel 198 and the third flow channel 199 is restored.

[0068] In more detail, Figure 2-Figure 5 As shown, the actuating mechanism includes an actuating motor 23 and a core shaft 27 which is transmission-connected to the actuating motor 23, and the ejection mechanism 22 includes cams (including at least a first cam 28, a second cam 29, a third cam 30, a fourth cam 31, and a fifth cam 32) arranged corresponding to the valves and a rod assembly corresponding to the cams and arranged perpendicular to the core shaft, the cams are fixed on the core shaft 27, and the actuating motor 23 is actuated to drive the core shaft 27 to rotate, thereby driving the cam on the core shaft 27 to rotate, and during the rotation of the core shaft 27, the raised portion of the cam will push the rod assembly forward, and by adjusting the distribution of the raised portion of the cam on the circumference of the core shaft 27, the rod assemblies corresponding to each cam are ejected in a preset order, so that the rod assembly is engaged or disconnected with the valve at the corresponding position, thereby realizing the disconnection or connection between the flow channels.

[0069] In a more specific embodiment, Figure 5 As shown, the rod assembly includes a push rod 221 whose one end contacts the protruding portion of the cam, and a push rod 222 which is sleeved on the other end of the push rod 221 to engage with the valve, wherein the end of the push rod 221 which contacts the protruding portion of the cam is formed as a boss having a diameter greater than that of the push rod body.

[0070] In some optional embodiments, the valve lock device 2 further includes a valve lock core fixing member 26, such as Figure 2 and Figure 3As shown, the core shaft 27 passes through the valve lock core fixing member 26, and one end of the core shaft 27 is rotatably connected to the valve lock core fixing member 26 through the first bearing 34, wherein the valve lock core fixing member 26 is also provided with a groove 261 corresponding to the rod assembly, a spring 223 is provided in the groove 261, and a through hole for the rod assembly to pass through is provided at the bottom of the groove 261, and the push rod 222 passes through the spring 223 and the through hole correspondingly, and one end of the spring 223 abuts against the boss of the push rod 221, and the other end abuts against the bottom of the groove 261. In this way, when the push rod 221 does not contact the protruding part of the cam, the push rod 222 can be reset under the action of the spring 223. Further, the actuating motor 23 can be fixed below the valve lock core fixing member 26 through the motor fixing member 231, and the motor fixing member 231 is fixed on one side of the valve lock core fixing member 26, and the other end of the core shaft 27 passes through the motor fixing member 231 and is rotatably connected to the motor fixing member 231 through the second bearing 35. In this way, by arranging the motor fixing and the valve lock core fixing on the same side of the instrument and arranging the actuating motor below the valve lock core fixing, the internal space can be fully utilized, making the valve lock device more compact, saving the internal space of the instrument, and thus reducing the size of the instrument.

[0071] In a more specific embodiment, the valve locking device 2 further comprises a transmission assembly 21 fixed on the motor fixing member 231, and the core shaft 27 is connected to the actuating motor 23 through the transmission assembly 21. Figure 2 and Figure 3 As shown, the transmission assembly 21 includes a driving wheel 211 connected to the actuating motor 23 and a driven wheel 213 connected to the core shaft 27, and the driving wheel 211 and the driven wheel 213 are connected by a synchronous belt 212. When the actuating motor 23 is braked, the driving wheel 211 and the driven wheel 213 of the transmission assembly 21 convert the kinetic energy of the actuating motor 23 into the rotation of the core shaft 27, thereby driving the rotation of the cam of the ejection mechanism 22 on the core shaft 27. Through the different rotation angles of the core shaft 27, the rod assemblies of the ejection mechanism 22 at different positions eject and block the corresponding valves.

[0072] In some optional embodiments, the valve locking device 2 also includes a code disk 25 connected to the core shaft 27 and a photoelectric component 24 cooperating with the code disk 25. The code disk 25 is preferably arranged at an end of the core shaft 27 away from the motor fixing part 231. The closed-loop control of the actuating motor is realized through the cooperation between the code disk 25 and the photoelectric component 24 to detect the rotation angle of the core shaft 27 and the cam, thereby controlling the rotation position of each cam so that it pushes out the rod assembly at the corresponding working station to close the corresponding valve.

[0073] Furthermore, if Figure 4As shown, a stopper 33 is preferably provided between the cam and the first bearing 34. Preferably, the stopper 33 is a stopper ring to prevent the first bearing 34 from rotating, resulting in loose connection between the spindle 27 and the motor fixing member 231.

[0074] In some preferred embodiments, in order to achieve a better sealing effect on the valve, the push rod 222 is preferably made of a soft material so that the push rod can more easily seal the valve and prevent it from leaking.

[0075] In some more preferred embodiments, the biological detection system further comprises a biological sample processing device control component 5 to apply a pressure difference between each chamber and the fluid channel of the biological sample processing device.

[0076] More specifically, the plurality of chambers of the biological sample processing device are respectively provided with plungers 20 at the top of the chambers for sealing the respective chambers, and the plungers 20 can move under the action of external force.

[0077] Corresponding to the plungers of each chamber, the biological sample processing device control component 5 may specifically include a plunger push rod assembly, which is arranged corresponding to the plungers 20 of each chamber of the biological sample processing device 1 and is configured to push / pull the corresponding plunger 20 in the corresponding sample processing process. For ease of understanding, in the subsequent description, the plunger push rod assembly is used to represent the biological sample processing device control component 5.

[0078] See also Figure 1 , Figure 6 and Figure 7 , corresponding to different processes of sample processing, at the corresponding working station of the ejection mechanism 22, the plunger pusher assembly pushes / pulls the corresponding plunger. In one embodiment, the plunger pusher assembly includes a lysis chamber plunger pusher state, a combination liquid chamber plunger pusher state, a cleaning liquid chamber plunger pusher state, and an elution liquid chamber plunger pusher state.

[0079] According to the following Figure 1-Figure 5 , explaining the working principle and process of the valve lock device 2 of the utility model:

[0080] The actuating motor 23 and the transmission assembly 21 drive the first cam 28 , the second cam 29 , the third cam 30 , the fourth cam 31 , the fifth cam 32 and the code disc 25 on the spindle 27 to rotate along the rotation direction of the motor.

[0081] During the rotation of the motor, the protruding parts on the first cam 28, the second cam 29, the third cam 30, the fourth cam 31, and the fifth cam 32 will push the push rod 221 forward toward the ejector 222. By adjusting the distribution of the protruding parts of the first cam 28, the second cam 29, the third cam 30, the fourth cam 31, and the fifth cam 32 on the circumference of the core shaft 27, the push rods 221 corresponding to the first cam 28, the second cam 29, the third cam 30, the fourth cam 31, and the fifth cam 32 can be ejected in a certain order, thereby realizing an ejection mechanism with a certain logic. For example, one or more push rods 221 can be ejected within the first rotation angle, and then another one or more push rods 221 can be ejected within the next rotation angle.

[0082] During the rotation of the motor, the code disk 25 on the core shaft 27 will rotate along with the core shaft 27. Through the cooperation between the code disk 25 and the photoelectric on the valve lock photoelectric panel, the position of the core shaft or cam rotation can be detected when rotating at a certain angle, forming a closed-loop motor control, thereby realizing the detection of each rotation angle to confirm the control logic of the ejection mechanism.

[0083] The following is further combined Figure 6 , Figure 7 and Figure 8 The valve lock device 2 is matched with the specific biological sample processing device 1 to illustrate the working process of the valve lock device 2 of the utility model with a specific embodiment. Figure 6 The blank space in the table indicates the situation of the rod assembly (elevator) corresponding to the concave side of the cam, and the gray space indicates the situation of the rod assembly (elevator) corresponding to the convex side of the cam. Among them, the first cam 28 to the fourth cam 31 correspond to one ejector rod respectively, and correspond to the first valve 191 to the fourth valve 194 respectively, and the fifth cam 32 corresponds to two ejectors, and corresponds to the fifth valve 195 and the sixth valve 196 respectively; reset means that the ejectors are not ejected and the valves are all open. In actual applications, the corresponding flow channels are located on the same surface (A surface) or on the opposite surface (B surface) of the valve according to actual conditions.

[0084] In the initial state, the valve locking device 2 of the biological sample processing device is in a reset state, that is, all valves are in an open state.

[0085] The biological sample testing process mainly includes the following steps:

[0086] Step 1: The core shaft 27 rotates to the first working position corresponding to the ejection mechanism 22. At this time, the ejector rods corresponding to the first cam 28, the second cam 29 and the fourth cam 31 are in the ejection state, so that the first valve 191, the second valve 192 and the fourth valve 194 are closed, the third cam 30 and the fifth cam 32 are concave corresponding rod assemblies, and the ejector rod 222 corresponding to the third cam 30 and the fifth cam 32 is in the non-ejection state, so that the third valve 193, the fifth valve 195 and the sixth valve 196 are opened, and the biological sample is ultrasonically lysed in the lysis chamber 11.

[0087] Step 2: the mandrel 27 rotates to the second station of the corresponding ejection mechanism 22, and at this time, all the ejector rods 222 are ejected by the cam, and the valve is completely closed. At this time, the plunger push injection assembly is located in the lysing chamber plunger push injection state, and the plunger push injection assembly pulls up the plunger 20 of the transition chamber 12, and pushes the plunger 20 of the lysing chamber 11. The solution after lysis in the lysing chamber 11 passes through the transition chamber 12, enters the binding liquid chamber 13 through the corresponding flow channel, and the nucleic acid in the binding liquid chamber 13 is captured by magnetic particles. Although the first valve 191 is in a closed state at this time, since the flow channel that the transition chamber 12 is connected to the binding liquid chamber 13 is located on the opposite side of the valve, even if the first valve 191 is in a closed state, the communication between the transition chamber 12 and the binding liquid chamber 13 will not be hindered.

[0088] Step 3: The core shaft 27 rotates to the third station corresponding to the ejection mechanism 22. At this time, the first cam 28 and the fourth cam 31 are concave corresponding rod assemblies, and the corresponding ejector rod 222 is in a non-ejection state. The second cam 29, the third cam 30 and the fifth cam 31 are convex corresponding rod assemblies, and the corresponding ejector rod 222 is in an ejection state, so that the first valve 191 and the fourth valve 194 are opened, and the second valve 192, the third valve 193, the fifth valve 195 and the sixth valve 196 are closed. At this time, the plunger pushing assembly is in the plunger pushing state of the binding liquid chamber. The plunger pushing assembly pushes the plunger 20 of the binding liquid chamber 13. The magnetic particles and binding liquid in the binding liquid chamber 13 will flow into the magnetic suction chamber 16 through the corresponding connected flow channels for the magnetic suction process. The excess binding liquid can flow into the waste liquid chamber 18 through the fourth valve 194 and the corresponding flow channel.

[0089] Step 4: The core shaft 27 rotates to the fourth station corresponding to the ejection mechanism 22, the second cam 29 and the fourth cam 31 are concave corresponding rod assemblies, and the corresponding ejector rod 222 is in a non-ejection state, the first cam 28, the third cam 30 and the fifth cam 32 are convex corresponding rod assemblies, and the corresponding ejector rod 222 is in an ejection state, so that the second valve 192 and the fourth valve 194 are opened. At this time, the plunger pushing assembly is in the plunger pushing state of the cleaning liquid chamber, and the plunger pushing assembly pushes the plunger 20 of the cleaning liquid chamber 14, and the cleaning liquid flows into the magnetic suction chamber 16 through the corresponding flow channel to clean the magnetic beads, and the excess cleaning liquid flows into the waste liquid chamber 18 through the fourth valve 194.

[0090] Step 5: The mandrel 27 rotates to the fifth station corresponding to the ejection mechanism 22, the first cam 28, the second cam 29 and the fifth cam 32 are convex corresponding rod assemblies, the corresponding ejector rod 222 is in the ejection state, the third cam 30 and the fourth cam 31 are concave corresponding rod assemblies, the corresponding ejector rod 222 is in the non-ejection state, so that the third valve 193 and the fourth valve 194 are opened. At this time, the plunger push assembly is in the plunger push state of the eluent chamber, the plunger push assembly pushes the plunger of the eluent chamber 15, the eluent (reconstitution solution) enters the magnetic suction chamber 16 to flush the flow channel and stays to elute the nucleic acid on the magnetic particles through heating, and the excess eluent (reconstitution solution) flows into the waste liquid chamber 18 through the fourth valve 194 and the corresponding flow channel.

[0091] Step 6: After the elution is completed, the core shaft 27 continues to rotate to the first station corresponding to the ejection mechanism 22. At this time, the first cam 28, the second cam 29 and the fourth cam 31 are convex corresponding rod assemblies, and the corresponding ejector rod 222 is in the ejection state. The third cam 30 and the fifth cam 32 are concave corresponding rod assemblies, and the ejector rod 222 is in the non-ejection state, so that the first valve 191, the second valve 192 and the fourth valve 194 are closed, and the third valve 193, the fifth valve 195 and the sixth valve 196 are opened. At this time, the plunger push assembly continues to push the plunger of the eluent chamber, and the eluent (reconstitution solution) flows into the reaction chamber 17 through the sixth valve 196 and the corresponding flow channel to reconstitute the freeze-dried balls.

[0092] Step 7: The core shaft 27 continues to rotate to the second station corresponding to the ejection mechanism 22. At this time, the plunger push assembly no longer moves, and the ejection mechanism is in a state where the ejector rod 222 is completely ejected by the cam and the valves are all closed. At this time, PCR, i.e., the nucleic acid amplification and detection step, is performed.

[0093] In the biological detection system of the utility model, the opening and closing of each valve of the biological sample processing device can be controlled by only one actuating motor through the valve locking device according to the different processing processes of the sample to be detected. The control method is simple and the production cost is saved. In addition, there are multiple ejection mechanisms corresponding to the multiple valves of the biological sample processing device one by one. The multiple ejection mechanisms are distributed along a straight line, and the structural layout is simple, which reduces the volume space occupied by the device for controlling the opening and closing of each valve of the biological sample processing device, and helps to miniaturize the instrument.

[0094] At this point, those skilled in the art should recognize that, although the present invention has been described with reference to preferred embodiments, various modifications may be made thereto and components thereof may be replaced with equivalents without departing from the scope of the present invention. In particular, the various technical features mentioned in the various embodiments may be combined in any manner as long as there is no structural conflict. The present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A biological detection system, characterized in that: include: A biological sample processing device, used to provide a fully enclosed place for nucleic acid extraction and amplification detection of a sample to be detected, which has multiple chambers, the multiple chambers at least including a sample lysis chamber, a cleaning chamber and an amplification detection chamber, and a fluid channel connecting the multiple chambers, and multiple valves are arranged between the fluid channels; A valve lock device, comprising an actuating mechanism and a plurality of ejection mechanisms actuated by the actuating mechanism and removably engaged with the valve, wherein the valve lock device is used to control the opening and closing of each valve in the biological sample processing device through the actuating mechanism and the ejection mechanism according to different processing processes of the sample to be detected, so as to control the flow or blocking of the sample to be detected between the plurality of chambers; A temperature control device, used to provide the temperature required during nucleic acid elution and amplification detection; An optical detection device, used for performing fluorescence detection on a sample to be detected; Wherein, the actuating mechanism comprises an actuating motor, and a plurality of the ejecting mechanisms are distributed along a straight line.

2. The biological detection system according to claim 1, characterized in that: Each ejection mechanism corresponds to a valve of the biological sample processing device and at least one working station, the working station corresponds to a corresponding process of sample processing, and the ejection mechanism of the working station simultaneously opens or closes the corresponding valve.

3. The biological detection system according to claim 2, characterized in that: The ejection mechanism is configured such that when the actuation mechanism is actuated once, the ejection mechanism undergoes M working stations in total as the actuation of the actuation mechanism occurs, and the M working stations are smaller than the N steps that the sample processing flow of the biological detection needs to go through.

4. The biological detection system according to claim 3, characterized in that: The M working stations include 5 stations, the N steps include at least 6 steps, and the steps at least include: sample lysis, combination of the lysis solution and magnetic particles, magnetic particle cleaning, elution, transfer of nucleic acid to be tested, and PCR reaction.

5. The biological detection system according to claim 3, characterized in that: The actuating mechanism also includes a core shaft drivingly connected to the actuating motor. The ejection mechanism is arranged corresponding to the valve and fixed on the core shaft, and is configured to rotate one circle with the core shaft. The ejection mechanism at the corresponding workstation controls the opening or closing of the valve.

6. The biological detection system according to claim 5, characterized in that: The ejection mechanism includes a cam arranged corresponding to the valve and a rod assembly arranged corresponding to the cam and perpendicular to the core shaft, the cam is fixed on the core shaft and rotates with the core shaft, and the rod assembly is configured so that when the cam rotates to a corresponding angle with the core shaft, the rod assembly at the corresponding workstation is ejected under the action of the raised part of the cam to engage with the corresponding valve.

7. The biological detection system according to claim 6, characterized in that: The rod assembly includes a push rod with one end in contact with the raised portion of the cam, and a push rod sleeved on the other end of the push rod to engage with the valve, and the end of the push rod in contact with the raised portion of the cam is formed as a boss with a diameter larger than that of the push rod body.

8. The biological detection system according to claim 7, characterized in that: The valve lock device also includes a valve lock core fixing part, the core shaft passes through the valve lock core fixing part and is rotatably connected to the valve lock core fixing part through a first bearing, wherein the valve lock core fixing part is also provided with grooves corresponding to the rod assemblies one by one, a spring is provided in the groove, a through hole is provided at the bottom of the groove for the rod assembly to pass through, the push rod passes through the spring and the through hole, one end of the spring abuts against the boss of the push rod, and the other end abuts against the bottom of the groove.

9. The biological detection system according to claim 8, characterized in that: The actuating motor is fixed below the valve lock core fixing member through a motor fixing member, and the motor fixing member is fixed to one side of the valve lock core fixing member. One end of the core shaft passes through the motor fixing member and is rotatably connected to the motor fixing member through a second bearing.

10. The biological detection system according to claim 9, characterized in that: The valve locking device also includes a code disk connected to the core shaft and a photoelectric component cooperating with the code disk. The code disk is fixed at the end of the core shaft opposite to the motor fixing part. The closed-loop control of the actuating motor is achieved through the cooperation between the code disk and the photoelectric component to detect the rotation angle of the core shaft and the cam.

11. The biological detection system according to claim 10, characterized in that: The valve locking device further comprises a transmission assembly fixed on the motor fixing member, and the core shaft is transmission-connected to the actuating motor through the transmission assembly. Wherein, the transmission assembly comprises a driving wheel connected to the actuating motor and a driven wheel connected to the core shaft respectively, and the driving wheel and the driven wheel are connected by a synchronous belt.

12. The biological detection system according to claim 7, characterized in that: The push rod is made of soft material.

13. The biological detection system according to claim 1, characterized in that: The biological detection system also includes a biological sample processing device control component to apply a pressure difference between each chamber and the fluid channel of the biological sample processing device.

14. The biological detection system according to claim 13, characterized in that: The multiple chambers of the biological sample processing device are respectively provided with plungers for sealing each chamber, and the biological sample processing device control component includes a plunger push rod component, which is arranged corresponding to the plungers of each chamber of the biological sample processing device and is configured to push / pull the corresponding plunger in the corresponding sample processing process.

15. The biological detection system according to claim 1, characterized in that: The biological detection system also includes an ultrasonic device, which abuts against the bottom of the sample lysis chamber and is used to emit ultrasonic waves to lyse the sample to be detected in the biological sample processing device.

16. The biological detection system according to claim 15, characterized in that: The biological sample processing device also includes a magnetic particle binding chamber, which is used to store magnetic particles and provide a place for the sample to be detected to bind with the magnetic particles; The biological detection system also includes a magnetic attraction component for providing an external magnetic field to attract the magnetic particles in the cleaning chamber of the biological sample processing device.

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