High Performance Low Cost MALDI MS-MS

Inactive Publication Date: 2008-11-06
VIRGIN INSTR CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0006]The invention comprises apparatus and methods for rapidly and accurately determining mass-to-charge ratios of molecular ions produced by a pulsed ionization source, and

Problems solved by technology

Time-of-flight (TOF) with reflecting analyzers provides excellent resolving power, mass accuracy, and sensitivity at lower masses (up to 5-10 kda), but performance is poor at higher masses primarily because of substantial fragmentation of ions in flight.
At higher masses, simple linear TOF analyzers provide satisfactory sensitivity, but resolving power and mass accuracy are low.
All of these improvements will h

Method used

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Embodiment Construction

[0038]A description of preferred embodiments of the invention follows. Referring now to FIG. 1. A MALDI sample plate 10 with samples of interest in matrix crystals on the surface is installed within an evacuated ion source housing 15 and a sample of interest is placed in the path of pulsed laser beam 60 which enters through a window 70 in the analyzer vacuum housing, and is reflected by mirror 65. As used herein, a “MALDI sample plate” or “sample plate” refers to the structure onto which the samples are deposited. Such sample plates are disclosed and described in copending U.S. application Ser. No. 11 / 541,467 filed Sep. 29, 2006, the entire disclosure of which is incorporated herein by reference.

[0039]At a certain or selected time following the laser pulse, a high-voltage pulse 12 (shown in FIG. 2) is applied to the sample plate 10 producing an electric field between sample plate 10 and extraction electrode 20 at ground potential causing a pulse of ions to be accelerated. The ions p...

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Abstract

The invention comprises apparatus and methods for rapidly and accurately determining mass-to-charge ratios of molecular ions produced by a pulsed ionization source, and for fragmenting all of the molecular ions produced and rapidly and accurately determining the intensities and mass-to-charge ratios of the fragments produced from each molecular ion.

Description

BACKGROUND OF THE INVENTION[0001]Many applications require accurate determination of the molecular masses and relative intensities of metabolites, peptides and intact proteins in complex mixtures. Time-of-flight (TOF) with reflecting analyzers provides excellent resolving power, mass accuracy, and sensitivity at lower masses (up to 5-10 kda), but performance is poor at higher masses primarily because of substantial fragmentation of ions in flight. At higher masses, simple linear TOF analyzers provide satisfactory sensitivity, but resolving power and mass accuracy are low. A TOF mass analyzer combining the best features of reflecting and linear analyzers is required for these applications.[0002]An important advantage of TOF mass spectrometry (MS) is that essentially all of the ions produced are detected, unlike scanning MS instruments. This advantage is lost in conventional MS-MS instruments where each precursor is selected sequentially and all non-selected ions are lost. This limita...

Claims

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Application Information

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IPC IPC(8): H01J49/26
CPCH01J49/40H01J49/401H01J49/0495H01J49/061H01J49/164
Inventor VESTAL, MARVIN L.
Owner VIRGIN INSTR CORP
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