Circuit structure for realizing synchronization of intercellular oscialltors

A circuit structure and oscillator technology, applied in the field of synthetic biology, can solve problems such as low efficiency, and achieve the effects of improving poor robustness, good anti-interference ability, and good synchronization effect

CN101475907AInactive Publication Date: 2009-07-08BEIHANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2009-07-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a circuit structure for realizing synchronization of intercellular repressilators, which consists of a repressilator circuit module, a synchronous signal generation module and an intercellular communication module, wherein the repressilator circuit module consists of a P[Ltet01]-cI phase inverter, a lambda P[R]-lacI phase inverter and a P[Llac01]-tetR phase inverter; output of the P[Ltet01]-cI phase inverter is connected with input of the lambda P[R]-lacI phase inverter of which the output is connected with input of the P[Llac01]-tetR phase inverter, and output of the P[Llac01]-tetR phase inverter is connected with input of input of the P[Ltet01]-cI phase inverter, thereby forming a circuit by end-to-end connection; the synchronous signal generation module consists of a P[BAD] promotor and an n gene; L-arabinose which is periodically injected from the outside is taken as input of the module; N proteins of output signals of the module are actuated on a t[L3] terminator of the intercellular communication module; the intercellular communication module consists of P[trp]-luxI phase inverters, the t[L3] terminator between the two P[trp]-luxI phase inverters, AHL and LuxR proteins; LuxI proteins of output signals of the P[trp]-luxI phase inverters are enzyme required for generating the AHL; and the generated AHL and AHL-LuxR complexes of the LuxR proteins are output signals of the module. As shown by simulation results, the circuit has better synchronization effect under the same conditions, and has stronger maneuverability.
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Description

(1) Technical field

[0001] The present invention relates to a synchronous circuit structure, in particular to a circuit structure for synchronizing repressilators between cells. The circuit structure is based on a coupling mechanism and external control means for synchronizing repressilator circuits between cells, and belongs to synthetic biology field of technology. (2) Background technology

[0002] In the past few centuries, the development of molecular biology and cell biology has shown explosive growth, and the understanding of life has also tended to be unified, that is, human beings can fully understand life processes and predict them, and even precisely control cell activities. With the accumulation of molecular biology technology, the development of genetic engineering and the combination of biology and engineering technology, synthetic biology began to appear and develop rapidly. As one of the fields of synthetic biology research, gene circuits help to solve probl...

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Embodiment Construction

[0026] See figure 1 , figure 2 , image 3 , Figure 4 As shown, a circuit structure that realizes intercellular oscillator (repressilator) synchronization is implemented as follows:

[0027] The present invention realizes a circuit structure for synchronizing oscillators between cells. The designed circuit structure is as follows: the circuit is composed of three parts: a repressilator circuit module, a synchronization signal generation module, and an intercellular communication module connecting the repressilator module and the synchronization signal generation module; The repressilator circuit module consists of P Ltet01 -cI, λP R -lacI, P Llac01 -tetR consists of three inverters, the cI gene is taken from lambda phage, the lacI gene is taken from Escherichia coli, and the tetR gene is taken from tetracycline, which can inhibit the transposon Tn10, P Ltet01 ,λP R ,P Llac01 They are the repressible promoters of cI gene, lacI gene and tetR gene respectively, P Ltet01...