Preparation method of fish collagen combined chitosan biological dressing

Abstract

The invention discloses a preparation method of a fish collagen combined chitosan biological dressing. The preparation method comprises the following steps: cutting clean fish wastes, from which surface impurities are removed, into fish meal of 50-200 meshes; putting the fish meal in an airtight container and then adding distilled water and a reaction enzyme into the airtight container, and stirring to form slurry; reacting the slurry under high pressure and ultrasonic treatment; after the reaction is finished, subjecting the slurry to purification treatment; adding chitosan to the purified slurry, heating until the temperature is in a range from 80 DEG C to 90 DEG C and stirring for 20-30 minutes; and finally, carrying out dialysis, concentration and drying, thereby obtaining the fish collagen combined chitosan biological dressing. The preparation method disclosed by the invention is simple in process, easy to implement, convenient to operate, and low in cost; the quickly extracted fish collagen is even in molecular weight distribution and few in impurities; the prepared biological dressing is good in mechanical strength, and has the effects of stopping bleeding and promoting wound healing, and also has the effect of antibiosis.

Description

technical field

[0001] The invention relates to the technical field of biological material preparation, in particular to a preparation method of fish collagen composite chitosan biological dressing. Background technique

[0002] Compared with traditional dressings, biological dressings have good compatibility, less toxic and side effects, low antigenicity, certain water permeability, can reduce the number of wound dressing changes, reduce the density of wound colonies, improve the quality of wound healing, and reduce the pain of patients, etc. . As a new type of medical wound dressing, biological dressings have been applied to the treatment of various skin wounds.

[0003] Collagen is an important structural protein with rich physiological functions in connective tissue. It has the advantages of low antigenicity, can be absorbed by the human body, and its degradation products have no toxic and side effects. Collagen can induce platelet attachment, produce a release reactio...

Examples

Embodiment 1

[0022] Cut the fish skin that has been cleaned of surface impurities into small pieces, and cut it into 50-mesh fish meal with a crusher; add distilled water and reaction enzyme that account for 3 times the mass of fish meal in a closed container to make a slurry, wherein the reaction enzyme is made from Composed of neutral lipase and bromelain, the amount of reaction enzyme added accounts for 0.1% of the fish meal mass, and the mass ratio of neutral lipase and bromelain is 1:4.

[0023] The prepared slurry was subjected to ultrasonic treatment at 1.5 atmospheric pressure and 15 kHz to control the reaction temperature at 50° C., pH at 7, and stir for 2 hours to carry out the enzymatic hydrolysis reaction. Add the sodium hydroxide that accounts for its quality 0.1% in the slurry after reaction, filter slurry and adjust filtrate pH value to be 5, add and account for the chitosan powder that solution gross mass is 1% in filtrate, wherein chitosan molecular weight is 300000. The ...

Embodiment 2

[0025] Cut the fish scales that have been cleaned of surface impurities into small pieces, and cut them into 100-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 5 times the mass of fish meal in a closed container to make a slurry, wherein the reaction enzymes are made from neutral It consists of lipase and alkaline protease, the amount of reaction enzyme added accounts for 0.3% of the fish meal quality, and the mass ratio of neutral lipase and alkaline protease is 1: 4.

[0026] The prepared slurry was treated under 2 standard atmospheric pressure and 20 kHz ultrasonic treatment to control the reaction temperature to 55° C., pH to 7.5, and stirred for 1.5 h to carry out the enzymatic hydrolysis reaction. Add the sodium hydroxide that accounts for its quality 0.2% in the slurry after reaction, filter slurry and adjust filtrate pH value to be 6, add and account for the chitosan powder that solution gross mass is 5% in filtrate, wherein chi...

Embodiment 3

[0028] Cut the swim bladder that has been cleaned of surface impurities into small pieces, and cut it into 150-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 7 times the mass of fish meal in a closed container to make a slurry, wherein the reaction enzymes are made of neutral It consists of lipase and trypsin, the amount of reaction enzyme added accounts for 0.5% of the mass of fish meal, and the mass ratio of neutral lipase to trypsin is 1:3.

[0029] The prepared slurry was subjected to 2.5 standard atmospheric pressure and 25 kHz ultrasonic treatment to control the reaction temperature to 70° C., pH to 7.5, and stirred for 1 hour to carry out the enzymatic hydrolysis reaction. Add the sodium hydroxide that accounts for its quality 0.3% in the slurry after reaction, filter slurry and adjust filtrate pH value to be 5, add and account for the chitosan powder that solution gross mass is 2% in filtrate, wherein chitosan molecular weight i...

technical field

[0001] The invention relates to the technical field of biological material preparation, in particular to a preparation method of fish collagen composite chitosan biological dressing. Background technique

[0002] Compared with traditional dressings, biological dressings have good compatibility, less toxic and side effects, low antigenicity, certain water permeability, can reduce the number of wound dressing changes, reduce the density of wound colonies, improve the quality of wound healing, and reduce the pain of patients, etc. . As a new type of medical wound dressing, biological dressings have been applied to the treatment of various skin wounds.

[0003] Collagen is an important structural protein with rich physiological functions in connective tissue. It has the advantages of low antigenicity, can be absorbed by the human body, and its degradation products have no toxic and side effects. Collagen can induce platelet attachment, produce a release reactio...

Examples

Embodiment 1

[0022] Cut the fish skin that has been cleaned of surface impurities into small pieces, and cut it into 50-mesh fish meal with a crusher; add distilled water and reaction enzyme that account for 3 times the mass of fish meal in a closed container to make a slurry, wherein the reaction enzyme is made from Composed of neutral lipase and bromelain, the amount of reaction enzyme added accounts for 0.1% of the fish meal mass, and the mass ratio of neutral lipase and bromelain is 1:4.

[0023] The prepared slurry was subjected to ultrasonic treatment at 1.5 atmospheric pressure and 15 kHz to control the reaction temperature at 50° C., pH at 7, and stir for 2 hours to carry out the enzymatic hydrolysis reaction. Add the sodium hydroxide that accounts for its quality 0.1% in the slurry after reaction, filter slurry and adjust filtrate pH value to be 5, add and account for the chitosan powder that solution gross mass is 1% in filtrate, wherein chitosan molecular weight is 300000. The ...

Embodiment 2

[0025] Cut the fish scales that have been cleaned of surface impurities into small pieces, and cut them into 100-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 5 times the mass of fish meal in a closed container to make a slurry, wherein the reaction enzymes are made from neutral It consists of lipase and alkaline protease, the amount of reaction enzyme added accounts for 0.3% of the fish meal quality, and the mass ratio of neutral lipase and alkaline protease is 1: 4.

[0026] The prepared slurry was treated under 2 standard atmospheric pressure and 20 kHz ultrasonic treatment to control the reaction temperature to 55° C., pH to 7.5, and stirred for 1.5 h to carry out the enzymatic hydrolysis reaction. Add the sodium hydroxide that accounts for its quality 0.2% in the slurry after reaction, filter slurry and adjust filtrate pH value to be 6, add and account for the chitosan powder that solution gross mass is 5% in filtrate, wherein chi...

Embodiment 3

[0028] Cut the swim bladder that has been cleaned of surface impurities into small pieces, and cut it into 150-mesh fish meal with a crusher; add distilled water and reaction enzymes that account for 7 times the mass of fish meal in a closed container to make a slurry, wherein the reaction enzymes are made of neutral It consists of lipase and trypsin, the amount of reaction enzyme added accounts for 0.5% of the mass of fish meal, and the mass ratio of neutral lipase to trypsin is 1:3.

[0029] The prepared slurry was subjected to 2.5 standard atmospheric pressure and 25 kHz ultrasonic treatment to control the reaction temperature to 70° C., pH to 7.5, and stirred for 1 hour to carry out the enzymatic hydrolysis reaction. Add the sodium hydroxide that accounts for its quality 0.3% in the slurry after reaction, filter slurry and adjust filtrate pH value to be 5, add and account for the chitosan powder that solution gross mass is 2% in filtrate, wherein chitosan molecular weight i...

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