Isaria fumosorosea wettable powder as well as preparation method and application thereof

A technology of Corydalis rosacea and wettable powder, which is applied in the field of Corridus rosacea wettable powder and its preparation, and can solve the problem that the control effect is greatly affected by environmental conditions, the control effect is unstable, and the adhesion is not good. and other problems, to achieve good application prospects, excellent performance indicators, and good control effects.

CN103749441AInactive Publication Date: 2014-04-30SOUTH CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-04-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses isaria fumosorosea wettable powder as well as a preparation method and an application thereof. The isaria fumosorosea wettable powder comprises the following components by mass percent: 5%-20% of isaria fumosorosea conidiophore powder, 3%-5% of wetting agent, 3%-5% of dispersing agent, 0.1%-0.5% of ultraviolet protective agent, and the balance of carrier. The isaria fumosorosea is an isaria fumosorosea strain SCAU-IFCF02 which is preserved in China Center for Type Culture Collection on November 1, 2013 with the preservation number CCTCCNO: M2013526. The isaria fumosorosea wettable powder is mainly used for preventing and treating brassicaceous vegetable pests, has good effect in preventing and treating pests such as plutella xylostella and the like, and has the advantages of high insecticidal activity, stable effect, lasting effect, small impact on environment, and convenience in use.
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Description

technical field

[0001] The invention relates to the field of pollution-free pesticide preparations. More specifically, it relates to a wettable powder of I. fumigatus and its preparation method and application. Background technique

[0002] Entomopathogenic fungi are an important part of insecticidal microorganisms, accounting for more than 60% of entomopathogenic microorganisms. The extensive development and utilization of microbial pesticides can effectively reduce and eliminate many negative effects such as residues, toxicity and drug resistance, which are common in current chemical pesticides. Rose smoke-colored Isospora ( Isaria fumosorosea ) is an important entomopathogenic fungus that can cause pest death. It is widely distributed in tropical, subtropical and temperate regions and has a wide range of hosts. There are 25 different families of insects recorded, including Homoptera, Diptera, Lepidoptera, A variety of vegetables, fruit trees and tea tree pests of the o...

Examples

Embodiment 1

[0045] Example 1 The preparation of rose fume-colored I. spp. conidia powder

[0046] The I. fumigatus used in the examples of the present invention is I. fumigatus strain SCAU-IFCF02, which was deposited in the China Center for Type Culture Collection on November 1, 2013, with a preservation number of CCTCC NO: M 2013526.

[0047] The preparation steps of the rose fume-colored clavula conidia powder of the present invention are as follows:

[0048] S1. First-level solid culture, take 50uL of the original bacterial liquid of I. fumigatus strain SCAU-IFCF02 and spread it on the SDAY solid medium (SDAY medium is the most suitable medium), and cultivate it at 24°C, the best culture The time is 15 days;

[0049] S2. Secondary liquid shaking culture, wash the spores on the SDAY solid medium with sterile water containing 0.03% Tween-80, and then filter with gauze to make 1×10 7 1 spore / mL of spore suspension, 100uL of the above spore suspension was inoculated into 200mL of Sabou...

Embodiment 2

[0057] Example 2 Optimal experiment of wetting agent for I. fumoscens wettable powder

[0058] 1. Test material

[0059] (1) The conidia powder prepared in Example 1.

[0060] (2) In this embodiment, the wetting agents involved in the present invention cannot be repeated here one by one, and the typical wetting agents summarized in the following several experimental processes are selected for illustration: SDBS (sodium dodecylbenzene sulfonate) ), SDS (sodium dodecyl sulfate), OP-10 (octylphenol polyoxyethylene ether-10), AEO-9 (fatty alcohol polyoxyethylene ether), penetrating agent T (Penetrating agent T) and penetrating agent agent JFC.

[0061] 2. Experimental method

[0062] (1) Determination of wetting force: Add each wetting agent into talcum powder at the ratio of 1%, 3% and 5%, and 10% of the rose smoke-colored I. Samples with different aerosol content but same spore content. The wetting time is measured according to the standard method of GB / T 5451-2001, th...

Embodiment 3

[0072] Example 3 Dispersant Optimum Experiment of I. fumoscens Wettable Powder

[0073] 1. Test material

[0074] (1) The conidia powder prepared in Example 1.

[0075] (2) In this embodiment, the dispersants involved in the present invention cannot be repeated here one by one, and the typical dispersants summarized in the following several experimental processes are selected for illustration: Sodium methylene dinaphthalene sulfonate (Diffusion promoter NNO ), sodium lignosulfonate (Sodium lignosulfonate), sodium polyacrylate (Sodium polyacrylate), dispersant MF (Diffusion promoter MF), dispersant P-19 and polyethylene glycol (Polyethylene glycol).

[0076] 2. Experimental method

[0077] (1) Determination of dispersibility: The screening of dispersants includes two processes of coarse sieving and fine sieving. Coarse sieving is that each dispersant is mixed with a sample containing 3% wetting agent OP-10, 10% spore powder, and 82% talcum powder at a ratio of 5% (w / w)...