A kind of multifunctional inorganic nano glue and preparation method thereof

Abstract

The invention provides a multifunctional inorganic nano glue and a preparation method thereof. Degradable nanoparticles with a concentration of 0.2-50% wt / v are added into a solvent; the degradable nanoparticles are element-doped mesopores containing disulfide bonds. Silicon nanoparticles SS-MSN or element-doped mesoporous bioactive glass MBG; solvents include but are not limited to ultrapure water, PBS or cell culture medium. The glue prepared by the invention has good biocompatibility, high bonding strength with biological tissues, and is biodegradable. The field of medicine includes, but is not limited to, adhesion and repair of skin, oral cavity, internal organs, intestinal tract, and meninges.

Description

technical field

[0001] The invention relates to a nano-glue and a preparation method thereof, which are used to solve the problem of single biological function of the existing tissue adhesive, and belong to the technical field of biological materials. Background technique

[0002] With the advent of the era of "trauma beauty", people not only require quick closure of wounds, but also eliminate scars. Traditional cosmetic sutures minimize scarring, but consume a lot of the surgeon's time. In the past ten years, the emergence of tissue adhesives is expected to solve the above problems, and can effectively replace surgical sutures or staples for the bonding of tension-free wounds, that is, first use absorbable sutures for subcutaneous suture to eliminate wound tension, and then use tissue. Adhesive replaces surgical sutures to bond the superficial skin, which can effectively eliminate scars. Compared with surgical sutures, tissue adhesives have the advantages of simple operat...

Examples

preparation example Construction

[0051] The present invention also provides a method for preparing the multifunctional nano-glue, wherein the preparation steps of the degradable mesoporous silica SS-MSN nano-glue are as follows:

[0052] Step 1. Preparation of degradable silica SS-SN: Dissolve cationic surfactant and sodium hydroxide in water, the concentration of cationic surfactant is 0.01-10% wt / v, and the concentration of sodium hydroxide is 0.01-10% wt / v 10% wt / v; add dropwise the mixture of disilicate and orthosilicate containing disulfide bonds, in the mixture V 含有二硫键的硅酸酯 :V 正硅酸酯 It is (0.005~0.95):1, the total concentration of the mixed solution in the cationic surfactant and sodium hydroxide aqueous solution is 0.05-20% v / v; react at 50-100 ℃ for 0.5-72h, with water and anhydrous in turn Wash with ethanol, collect the precipitate by centrifugation, and vacuum dry to obtain solid powder SS-MSN;

[0053]Step 2. Preparation of degradable mesoporous silica SS-MSN: redisperse the solid powder SS-SN in a...

example 1

[0068] First, 1% wt / v cetyltrimethylammonium bromide was dissolved in deionized water, and 0.5% wt / v sodium hydroxide was added to provide an alkaline environment. After stirring at 50 °C until complete dissolution, 1 % wt / v copper nitrate and 3% wt / v magnesium nitrate solution, then slowly dropwise addition of ethyl orthosilicate and bis-(triethoxysilylpropyl) disulfide (volume ratio 1:1, total concentration 3 %v / v), continue to stir the product obtained by the reaction, wash with deionized water and ethanol respectively after centrifugation; use 10%wt / v ethanol solution containing ammonium nitrate to extract and reflux for 24h to remove cetyltrimethyl bromide ammonium chloride, fully washed with deionized water and absolute ethanol after centrifugation, and vacuum dried overnight; prepare a 10% wt / v PBS solution to obtain copper and magnesium two-element doped SS-MSN nano-glue.

example 2

[0070] Dissolve 10% wt / v dodecyl trimethyl ammonium bromide in deionized water, add 5% wt / v sodium hydroxide to provide an alkaline environment, stir at 80 °C until completely dissolved, and then immediately add 0.1 % wt / v copper nitrate, 0.2% wt / v strontium nitrate and 1.2% wt / v cerium nitrate solution, followed by slow dropwise addition of ethyl orthosilicate and 4,4,17,17-tetraethoxy-3,18 -Dioxin-10,11-dithio-4,17-disilaicosane (volume ratio 7:3, total concentration 7% v / v), continue to stir the product obtained by the reaction, and use it up after centrifugation Fully washed with ionized water and ethanol; extracted and refluxed with a mixed solution containing hydrochloric acid and ethanol (50% wt / v) for 8 h to remove dodecyltrimethylammonium bromide, and the product was fully washed with deionized water and anhydrous ethanol, Vacuum dry overnight; prepare a 20% wt / v medium solution to obtain copper, strontium, and cerium tri-element-doped SS-MSN nano-glue.

technical field

[0001] The invention relates to a nano-glue and a preparation method thereof, which are used to solve the problem of single biological function of the existing tissue adhesive, and belong to the technical field of biological materials. Background technique

[0002] With the advent of the era of "trauma beauty", people not only require quick closure of wounds, but also eliminate scars. Traditional cosmetic sutures minimize scarring, but consume a lot of the surgeon's time. In the past ten years, the emergence of tissue adhesives is expected to solve the above problems, and can effectively replace surgical sutures or staples for the bonding of tension-free wounds, that is, first use absorbable sutures for subcutaneous suture to eliminate wound tension, and then use tissue. Adhesive replaces surgical sutures to bond the superficial skin, which can effectively eliminate scars. Compared with surgical sutures, tissue adhesives have the advantages of simple operat...

Examples

preparation example Construction

[0051] The present invention also provides a method for preparing the multifunctional nano-glue, wherein the preparation steps of the degradable mesoporous silica SS-MSN nano-glue are as follows:

[0052] Step 1. Preparation of degradable silica SS-SN: Dissolve cationic surfactant and sodium hydroxide in water, the concentration of cationic surfactant is 0.01-10% wt / v, and the concentration of sodium hydroxide is 0.01-10% wt / v 10% wt / v; add dropwise the mixture of disilicate and orthosilicate containing disulfide bonds, in the mixture V 含有二硫键的硅酸酯 :V 正硅酸酯 It is (0.005~0.95):1, the total concentration of the mixed solution in the cationic surfactant and sodium hydroxide aqueous solution is 0.05-20% v / v; react at 50-100 ℃ for 0.5-72h, with water and anhydrous in turn Wash with ethanol, collect the precipitate by centrifugation, and vacuum dry to obtain solid powder SS-MSN;

[0053]Step 2. Preparation of degradable mesoporous silica SS-MSN: redisperse the solid powder SS-SN in a...

example 1

[0068] First, 1% wt / v cetyltrimethylammonium bromide was dissolved in deionized water, and 0.5% wt / v sodium hydroxide was added to provide an alkaline environment. After stirring at 50 °C until complete dissolution, 1 % wt / v copper nitrate and 3% wt / v magnesium nitrate solution, then slowly dropwise addition of ethyl orthosilicate and bis-(triethoxysilylpropyl) disulfide (volume ratio 1:1, total concentration 3 %v / v), continue to stir the product obtained by the reaction, wash with deionized water and ethanol respectively after centrifugation; use 10%wt / v ethanol solution containing ammonium nitrate to extract and reflux for 24h to remove cetyltrimethyl bromide ammonium chloride, fully washed with deionized water and absolute ethanol after centrifugation, and vacuum dried overnight; prepare a 10% wt / v PBS solution to obtain copper and magnesium two-element doped SS-MSN nano-glue.

example 2

[0070] Dissolve 10% wt / v dodecyl trimethyl ammonium bromide in deionized water, add 5% wt / v sodium hydroxide to provide an alkaline environment, stir at 80 °C until completely dissolved, and then immediately add 0.1 % wt / v copper nitrate, 0.2% wt / v strontium nitrate and 1.2% wt / v cerium nitrate solution, followed by slow dropwise addition of ethyl orthosilicate and 4,4,17,17-tetraethoxy-3,18 -Dioxin-10,11-dithio-4,17-disilaicosane (volume ratio 7:3, total concentration 7% v / v), continue to stir the product obtained by the reaction, and use it up after centrifugation Fully washed with ionized water and ethanol; extracted and refluxed with a mixed solution containing hydrochloric acid and ethanol (50% wt / v) for 8 h to remove dodecyltrimethylammonium bromide, and the product was fully washed with deionized water and anhydrous ethanol, Vacuum dry overnight; prepare a 20% wt / v medium solution to obtain copper, strontium, and cerium tri-element-doped SS-MSN nano-glue.