A kind of high-efficiency breeding method of Lanzhou lily seedlings

Abstract

The invention discloses a high-efficiency breeding method for Lanzhou lily seedlings. The Lanzhou lily seedlings are used as explants, and the seedlings are subjected to the steps of seed collection, sterilization, germination, at least one propagation culture, strong seedling culture and bulb expansion culture. , to prepare lily seedlings. The beneficial effects of the invention are as follows: the invention uses seed germination instead of scale cuttings to produce seedlings, blocks the intergenerational spread and accumulation of viruses caused by scale cuttings, and significantly reduces the viral load of lily seedlings compared with the prior art , improve the heterogeneity of the seedling population, and effectively solve the defects of virus accumulation and narrow genetic background caused by asexual reproduction in Lanzhou lily seedling propagation. The breeding method can be used for the preservation of Lanzhou lily germplasm resources and the factory breeding of seedlings, which is beneficial to the breeding of new Lanzhou lily varieties, the rapid popularization of excellent varieties, the preservation and innovation of germplasm resources, and the improvement of the yield and quality of Lanzhou lily. .

Description

technical field

[0001] The invention relates to the technical field of lily seedling bulb breeding, in particular to an efficient breeding method of Lanzhou lily seedlings. Background technique

[0002] Lanzhou lily has long propagated bulbs by scale cuttings and cauline small bulbs. There is a problem of breed degradation caused by the accumulation and transmission of viruses, which seriously affects the quality and yield of Lanzhou lily, and restricts the development of Lanzhou lily industry. According to the survey, although the natural fruit setting rate of Lanzhou lily is only 7.15% on average, the number of seeds in the Lanzhou lily capsule is large, each capsule contains about 200 to 250 seeds, and the filling rate is 30 to 67.6%. SUMMARY OF THE INVENTION

[0003] The purpose of the present invention is to aim at the above-mentioned defects in the prior art, and provide a kind of Lanzhou lily seedling efficient breeding method.

[0004] In order to achieve the abov...

Examples

Embodiment 1

[0027] A method for efficient breeding of Lanzhou lily seedlings, comprising the following steps:

[0028] 1) seed seed collection: collect the Lanzhou lily capsule after the flower, remove the pericarp to obtain the seed seed; the collected Lanzhou lily capsule is the more than 50 days after the flower capsule;

[0029] 2) sterilization of seed seeds: after washing the seed seeds containing embryos obtained in step 1), soak them with ethanol solution and sodium hypochlorite solution respectively, then wash with sterile water, and dry the water with sterile filter paper, that is, to obtain a degerming solution. Bacteria seed; first rinse the seed with clean water for 9-11min, then soak it in ethanol solution with a concentration of 70% by volume for 40-50s, and then soak it in a sodium hypochlorite solution with a mass volume ratio of 2% for 10-20min, and finally Washed with sterile water for 3 times, and dried with sterile filter paper, the sterile seeds can be obtained;

[...

Embodiment 2

[0037] like Figure 1 to Figure 5 As shown, it is a process diagram of taking the Lanzhou lily seed as the explant through debacterial treatment, embryo germination, proliferation culture, strong seedling cultivation and small bulb expansion culture in the present invention.

[0038] 1. Debacterial treatment and germination induction of Lanzhou lily seeds:

[0039] Sterilize the seeds: first rinse with running water for 20 min, soak in 70% (v / v) ethanol for 1 min, then soak in 2% (v / v) NaClO solution for 20 min, and finally wash with sterile water 3 times. Get sterile seeds. The sterile seeds were equally divided into 3 parts, with concentrations of 0 and 250 mg·L, respectively. -1 , 500 mg·L -1 (w / v) gibberellin (GA 3 ) solution was soaked for 24 h, inoculated on 1 / 2 MS medium for dark culture (23-25 ​​°C), and each treatment was repeated three times. The germination rate was counted after 13 days. Table 1 shows GA 3 Effects on the germination rate of Lanzhou lily seed...

technical field

[0001] The invention relates to the technical field of lily seedling bulb breeding, in particular to an efficient breeding method of Lanzhou lily seedlings. Background technique

[0002] Lanzhou lily has long propagated bulbs by scale cuttings and cauline small bulbs. There is a problem of breed degradation caused by the accumulation and transmission of viruses, which seriously affects the quality and yield of Lanzhou lily, and restricts the development of Lanzhou lily industry. According to the survey, although the natural fruit setting rate of Lanzhou lily is only 7.15% on average, the number of seeds in the Lanzhou lily capsule is large, each capsule contains about 200 to 250 seeds, and the filling rate is 30 to 67.6%. SUMMARY OF THE INVENTION

[0003] The purpose of the present invention is to aim at the above-mentioned defects in the prior art, and provide a kind of Lanzhou lily seedling efficient breeding method.

[0004] In order to achieve the abov...

Examples

Embodiment 1

[0027] A method for efficient breeding of Lanzhou lily seedlings, comprising the following steps:

[0028] 1) seed seed collection: collect the Lanzhou lily capsule after the flower, remove the pericarp to obtain the seed seed; the collected Lanzhou lily capsule is the more than 50 days after the flower capsule;

[0029] 2) sterilization of seed seeds: after washing the seed seeds containing embryos obtained in step 1), soak them with ethanol solution and sodium hypochlorite solution respectively, then wash with sterile water, and dry the water with sterile filter paper, that is, to obtain a degerming solution. Bacteria seed; first rinse the seed with clean water for 9-11min, then soak it in ethanol solution with a concentration of 70% by volume for 40-50s, and then soak it in a sodium hypochlorite solution with a mass volume ratio of 2% for 10-20min, and finally Washed with sterile water for 3 times, and dried with sterile filter paper, the sterile seeds can be obtained;

[...

Embodiment 2

[0037] like Figure 1 to Figure 5 As shown, it is a process diagram of taking the Lanzhou lily seed as the explant through debacterial treatment, embryo germination, proliferation culture, strong seedling cultivation and small bulb expansion culture in the present invention.

[0038] 1. Debacterial treatment and germination induction of Lanzhou lily seeds:

[0039] Sterilize the seeds: first rinse with running water for 20 min, soak in 70% (v / v) ethanol for 1 min, then soak in 2% (v / v) NaClO solution for 20 min, and finally wash with sterile water 3 times. Get sterile seeds. The sterile seeds were equally divided into 3 parts, with concentrations of 0 and 250 mg·L, respectively. -1 , 500 mg·L -1 (w / v) gibberellin (GA 3 ) solution was soaked for 24 h, inoculated on 1 / 2 MS medium for dark culture (23-25 ​​°C), and each treatment was repeated three times. The germination rate was counted after 13 days. Table 1 shows GA 3 Effects on the germination rate of Lanzhou lily seed...

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