Skin care composition and toner containing plant essence water

Abstract

The present invention relates to the technical field of skin care products, and its IPC classification number is A61K8 / 98, and in particular, it relates to a skin care composition and toner containing plant essence water. The skin care composition comprises a plant extract and a plant essence water, and the plant essence water can achieve at least one of the following beneficial effects: (1) effectively preventing and treating blackheads / acne; (2) improving permeability; (3) having a refreshing skin feeling; (4) promoting cell division and improving cell survival rate; (5) having moisturizing properties. The skin care composition and toner containing plant essence water provided by the present invention can effectively prevent and treat blackheads / acne, have good permeability and refreshing skin feeling, and can also improve cell survival rate.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and its IPC classification number is A61K8 / 98, in particular to a skin care composition and toner containing plant essence water. Background Art

[0002] Due to environmental pollution, computer radiation, irregular work and rest and other factors, people's skin has been damaged. Common symptoms include acne, pimples, and pimples. People often use skin care products to alleviate or eliminate these symptoms. Common skin care products include toners, lotions, essences, creams, etc. These skin care products often contain some chemical nutrients or moisturizing substances such as glycerin, which makes these skin care products often feel greasy during use.

[0003] In recent years, skin care products containing natural functional ingredients have been favored by consumers. These natural functional ingredients are basically derived from plant extracts. A large amount of condensed water is formed during the production process of plant extracts, which contains a large number of small molecule volatile components and has certain active functions. At present, manufacturers generally treat it as waste. If it is directly discharged, it will cause certain environmental pollution; if it is purified, it will increase costs.

[0004] With the improvement of people's living standards and aesthetic standards, many women of different levels and ages also admire fair and clean skin, and put forward higher and higher requirements for safe and healthy skin care products. "Lack of water" is one of the main culprits of skin deterioration and aging. Moisturizing is not only for sensitive skin. All kinds of skin will lack water due to factors such as environment, age, nutrition, and health. When the moisture content of the stratum corneum of human skin is less than 10%, the skin will lose its softness and elasticity, and then become rough, dry, and various skin abnormalities will occur. If it is not corrected for a long time, the skin is prone to aging, spots and sensitive. Therefore, after washing the face, using toner can quickly replenish water. Most of the toners in the prior art have a single effect, are suitable for people with specific skin characteristics, and have general effects, and are not suitable for promotion and use. Therefore, it is very necessary to provide a multi-functional skin toner that is simple in process, safe, non-toxic and side-effect-free, and has the effects of long-term moisturizing, refreshing and non-greasy, good isolation effect, promoting skin regeneration, anti-inflammatory, antibacterial and antiseptic, and suitable for promotion and application. At the same time, the chemical additives added in their skin care products, especially toners, may have side effects, pose great safety risks, and may also hinder the functioning of the ingredients in the plant extracts in the toners. Therefore, the selection of plant extracts is crucial. Summary of the invention

[0005] A first aspect of the present invention provides a skin care composition comprising plant essence water, wherein the plant essence water comprises at least one of ganoderma lucidum spore powder essence water, dendrobium officinale essence water, ganoderma lucidum essence water, lavender essence water, nutmeg essence water, agarwood essence water, dalbergia odorifera essence water, clove essence water, rose essence water, hibiscus essence water, chrysanthemum essence water, peony essence water, aster essence water, gynostemma pentaphyllum essence water, clover essence water, saffron petal essence water, fritillaria essence water, atractylodes macrocephala essence water, corydalis yanhusuo essence water, Scrophularia ningpoensis essence water, Ophiopogon japonicus essence water, Curcuma aromatica essence water, and tea tree essence water.

[0006] Plant Essence Water

[0007] The plant essence water is obtained by condensation, which refers to a large amount of condensed water formed in the production process of plant extracts (including essential oils). The condensed water also contains a large amount of small molecular volatile components and has certain activity.

[0008] The plant essence waters that can be listed include Ganoderma lucidum spore powder essence water, Dendrobium officinale essence water, Ganoderma lucidum essence water, lavender essence water, nutmeg essence water, agarwood essence water, dalbergia odorifera essence water, clove essence water, rose essence water, hibiscus essence water, chrysanthemum essence water, peony essence water, aster essence water, Gynostemma pentaphyllum essence water, Tripterygium wilfordii essence water, saffron petal essence water, Fritillaria thunbergii essence water, Atractylodes macrocephala essence water, Corydalis yanhusuo essence water, Scrophularia ningpoensis essence water, Ophiopogon japonicus essence water, Curcuma aromatica essence water, tea tree essence water, etc., and the plant essence water of the present invention is not limited to other plant essence waters.

[0009] For example, Ganoderma lucidum essence water is a large amount of condensed water formed during the production process of Ganoderma lucidum extract, which also contains a large amount of active substances.

[0010] The plant essence water can achieve at least one of the effects of effectively preventing and treating blackheads / acne, improving permeability, having a refreshing skin feeling, promoting cell division and improving cell survival rate, and moisturizing.

[0011] Preferably, the plant essence water is at least one of Dendrobium officinale essence water, Ganoderma lucidum essence water, Ganoderma lucidum spore powder essence water, lavender essence water, hibiscus essence water, and Gynostemma pentaphyllum essence water.

[0012] During the research process, the applicant discovered that when the skin care composition includes at least one of Dendrobium officinale essence water, Ganoderma lucidum essence water, lavender essence water, hibiscus essence water, and Gynostemma pentaphyllum essence water, the composition has one or more functions of preventing and treating acne / pimples, improving permeability, refreshing skin feel, promoting cell division and improving cell survival rate, moisturizing, and antibacterial.

[0013] Further preferably, the plant essence water is Ganoderma lucidum essence water.

[0014] In the present invention, the ganoderma essence water is prepared according to the following preparation method, but is not limited to the following preparation method: taking plant water extract, concentrating it by vacuum concentration, and collecting condensed water to obtain the essence water.

[0015] Plant Extracts

[0016] Plant extracts refer to solid, viscous or liquid products extracted or processed from plants (all or part of plants) using appropriate solvents or methods. The plant extracts can be purchased directly from the market, or can be prepared by making them yourself, or by purchasing semi-finished products from the market. The plant extracts can be extracted using any extraction method, as long as the products suitable for the present invention containing the separated active ingredients of plant substances are extracted.

[0017] The plant extracts that can be cited include lavender extract, chrysanthemum extract, honeysuckle extract, peony extract, dendrobium candidum extract, agarwood extract, hibiscus extract, thyme extract, herbaceous dysentery extract, clove extract, gynostemma pentaphyllum extract, ganoderma lucidum spore powder extract, ganoderma lucidum extract, clover extract, tomato petal extract, fritillaria extract, atractylodes macrocephala extract, corydalis extract, Scrophularia ningpoensis extract, Ophiopogon japonicus extract, Curcuma aromatica extract, etc., and the plant extracts of the present invention are not limited to other plant extracts.

[0018] Preferably, the plant extract includes chrysanthemum extract and honeysuckle extract.

[0019] Further preferably, the weight of the chrysanthemum extract is 0.05%-2% of the weight of the skin care composition; the weight of the honeysuckle extract is 0.05%-2% of the weight of the skin care composition.

[0020] Further preferably, the weight of the chrysanthemum extract is 0.1%-1% of the weight of the skin care composition; the weight of the honeysuckle extract is 0.1%-1% of the weight of the skin care composition.

[0021] More preferably, the weight of the chrysanthemum extract is 0.8% by weight of the skin care composition; the weight of the honeysuckle extract is 0.8% by weight of the skin care composition.

[0022] Chrysanthemum extract is extracted from chrysanthemum, which is the dried capitula of the chrysanthemum plant of the Asteraceae family. The "Pharmacopoeia of the People's Republic of China" (2020 edition) records that it has the effects of dispersing wind and heat, calming the liver and improving eyesight, clearing heat and detoxifying. It is mainly used to treat wind-heat colds, red and swollen eyes, furuncles, carbuncles, etc.

[0023] Honeysuckle extract is extracted from honeysuckle, which is the dried buds or initially opened flowers of the Caprifoliaceae plant Lonicera japonica. As a traditional Chinese herbal medicine with a long history, it is listed as a top-grade product in the "Shennong's Herbal Classic". It has many functions such as antiviral, anti-tumor, immunity-enhancing and anti-oxidation.

[0024] The main component of the plant extract of the present invention is one of polysaccharide, saponin and flavonoid.

[0025] The applicant unexpectedly discovered that when the plant extracts include chrysanthemum extract and honeysuckle extract, acne and pimple-causing skin can be effectively treated, but the effect is slow. In order to solve the problem of slow effect, a large amount of surfactants are often added to the system in the prior art. The addition of surfactants can easily cause excessive degreasing of the skin surface, thereby drying the skin, and at the same time aggravate facial acne, causing facial infection, which cannot be healed for a long time and has a poor skin feel.

[0026] More preferably, the plant extract also includes Gynostemma pentaphyllum extract.

[0027] Further preferably, the weight of the Gynostemma pentaphyllum extract is 0.05%-1% by weight of the skin care composition.

[0028] More preferably, the weight of the Gynostemma pentaphyllum extract is 0.3% by weight of the skin care composition.

[0029] Gynostemma pentaphyllum extract is also known as gynostemma saponin. Gynostemma pentaphyllum extract is separated from Gynostemma pentaphyllum, which is a herbaceous climbing plant of the Gynostemma pentaphyllum family and the genus Gynostemma. It is cold in nature and bitter in taste. It has the effects of clearing away heat and detoxifying, relieving cough, clearing the lungs and removing phlegm, nourishing the heart and calming the nerves, and replenishing qi and producing essence.

[0030] In addition, the applicant unexpectedly discovered that when a certain amount of plant extracts, such as gypenosides and ganoderma lucidum essence water, were added, the recovery time of acne-caused skin was unexpectedly shortened. The applicant speculated that the possible reason was that the gypenosides molecules included tetracyclic triterpene dammarane-type saponin aglycones and side chain sugars, and the side chain sugars were mainly monosaccharides, disaccharides and trisaccharides composed of glucose, arabinose, rhamnose and xylose, which increased the emulsification, dispersion and wetting functions of the composition including chrysanthemum extract and honeysuckle extract. At the same time, combined with the small molecule active substances unique to the ganoderma lucidum essence water, its penetration into the skin was enhanced, and the skin could convert the absorbed gypenosides into ginsenosides for human use.

[0031] More preferably, the plant extract further comprises a Dendrobium officinale extract, which accounts for 0.05-1% by weight of the skin care composition.

[0032] More preferably, the plant extract further comprises a Dendrobium officinale extract, which accounts for 0.05-0.2% by weight of the skin care composition.

[0033] More preferably, the plant extract also includes a Dendrobium officinale extract, which accounts for 0.1% by weight of the skin care composition.

[0034] In one embodiment, the weight of gypenosin is 10%-50% of the weight of the plant extract, the weight of Dendrobium officinale polysaccharide is 10%-40% of the weight of the plant extract, and the weight of other plant extracts is 10%-80% of the weight of the plant extract, and the specific proportions are not limited.

[0035] The applicant found that the addition of gypenosides caused the composition to easily generate foam, especially in the process of transportation, more foam was generated, which affected the normal use of the composition. The applicant unexpectedly found that when the plant extract also included a Dendrobium officinale extract, the height of the foam could be significantly reduced, which greatly increased the use value and storage\transportation value of the composition. The applicant believes that the possible reason is that the saponins in gypenosides are mostly triterpenoid saponins, which contain a large number of cyclic hydroxyl groups in their molecular structure, and the dendrobium polysaccharides in the Dendrobium officinale extract and the dendrobium nobile containing dendrobium alkaloids, dendrobium amine, dendrobium alkaloids, dendrobium star alkaloids, dendrobium alkaloids, dendrobium alkaloids, 6-hydroxydendrobium star alkaloids and other molecules change the distribution state of the triterpenoid saponins in the gypenosides and intermolecular, so that the surface tension of the composition containing the Dendrobium officinale extract and gypenosides is reduced, so that the foam is reduced.

[0036] The preparation methods of plant extracts that can be cited can be:

[0037] (1) Decolorization of raw materials: soak the raw materials in 5 to 20 times the volume of 80% to 100% ethanol for 8 to 24 hours, filter, drain the excess ethanol and air dry naturally;

[0038] (2) Extraction: water extraction, solid-liquid ratio 1:5-20; extraction times 1-3 times, each extraction time 0.5-2 hours;

[0039] (3) Decolorization of the extract: Add 2% to 10% activated carbon to the extract, perform dynamic adsorption, filter, remove the activated carbon, and obtain a clarified extract;

[0040] (4) Concentration: The decolorized extract is concentrated, and the vacuum degree of concentration is preferably -0.09 to -0.07 MPa, and the concentration temperature is preferably 50 to 80° C.;

[0041] (5) Alcohol precipitation: Add 2 to 5 times the volume of the concentrate to 95% ethanol for precipitation (add while stirring), cover the lid, and let it stand at low temperature overnight to obtain the precipitation solution for later use;

[0042] (6) Drying: Centrifuge the precipitate obtained by alcohol precipitation at 4000 rpm for 5-15 minutes; remove the supernatant, and use hot air drying or freeze drying to obtain the plant extract.

[0043] The method in the above steps can omit different steps according to different plants and the effective components to be enriched.

[0044] The preparation method of the plant extract may also be the following preparation steps:

[0045] (1) Dissolving: Take a powdered crude plant extract, add 100 times purified water and fully dissolve it at 65°C to 90°C to obtain a plant extract solution for later use;

[0046] (2) Alcohol precipitation: Take the plant extract solution, add 95% ethanol in a volume ratio of 1:3 (stirring), cover the lid, and let it stand at low temperature overnight to obtain the precipitate solution for later use;

[0047] (3) Centrifuging and drying the alcohol precipitate: centrifuging the precipitate obtained by alcohol precipitation at 4000 rpm for 5-15 minutes; removing the supernatant, and drying the precipitate by hot air drying or freeze drying to obtain a plant extract containing polysaccharides.

[0048] In an embodiment of the present invention, the preparation method of chrysanthemum extract is:

[0049] (1) Primary decolorization: Soak chrysanthemum in 10 times the volume of 95% ethanol overnight, filter, dry naturally in the sun, evaporate the ethanol, and set aside;

[0050] (2) Preparation of extract: Take the decolorized raw material once, extract it twice with purified water, with the solid-liquid ratio of 15 times and 10 times respectively, extract for 1 hour each time, filter, and combine the filtrate to obtain the chrysanthemum extract;

[0051] (3) Decolorization: Take chrysanthemum extract, add activated carbon powder, and perform dynamic adsorption decolorization for more than 4 hours, and filter through a 0.45 μL filter membrane to obtain chrysanthemum extract;

[0052] (4) Alcohol precipitation: Take the chrysanthemum extract, add 95% ethanol in a volume ratio of 1:3 (stirring), cover the lid, and let it stand at low temperature overnight to obtain the chrysanthemum precipitate, which is set aside;

[0053] (5) Drying: centrifuge the chrysanthemum precipitate at 4000 rpm for 10 minutes; remove the supernatant and dry the precipitate to obtain the chrysanthemum extract.

[0054] The preparation methods of the honeysuckle extract and the chrysanthemum extract are the same.

[0055] In the present invention, the preparation method of the Dendrobium officinale extract is:

[0056] (1) Dissolving: Take a crude extract of Dendrobium officinale, add 100 times purified water and fully dissolve it at 65° C. to 90° C. to obtain a Dendrobium officinale aqueous solution for later use;

[0057] (2) Alcohol precipitation: Take the Dendrobium officinale aqueous solution, add 95% ethanol in a volume ratio of 1:3 (stirring while adding), cover the lid, and let it stand at low temperature overnight to obtain the Dendrobium officinale alcohol precipitation solution, which is set aside;

[0058] (3) Drying: centrifuging the alcohol precipitate of Dendrobium officinale at 4000 rpm for 10 minutes; removing the supernatant to obtain a precipitate, which is dried to obtain the Dendrobium officinale extract containing polysaccharides.

[0059] The Gynostemma pentaphyllum extract described in the examples of the present invention is commercially available.

[0060] The preparation method of the skin care composition can be listed as follows:

[0061] (1) Divide the plant essence water into four groups: A, B, C, and D, wherein the weight ratio of A, B, C, and D is 5:5:1:1;

[0062] (2) mixing the plant essence water of group A with chrysanthemum extract at 65° C. to obtain A1, mixing the plant essence water of group B with honeysuckle extract at 65° C. to obtain B1, mixing the plant essence water of group C with Dendrobium officinale extract at 65° C. to obtain C1, and mixing the plant essence water of group D with Gynostemma pentaphyllum extract at 65° C. to obtain D1;

[0063] (3) A1, B1, C1 and D1 are mixed evenly to obtain a skin care composition containing plant essence water.

[0064] The preparation method of the skin care composition can also be: mixing the plant extracts evenly, and then mixing them evenly with the essence water.

[0065] The second aspect of the present invention provides a toner, which comprises a skin care composition of any one of the above-mentioned essence waters.

[0066] In one embodiment, the toner also includes plant essence water-1.

[0067] The plant essence water-1 may be the same as or different from the plant essence water.

[0068] Preferably, the mass concentration of the plant extract in the skin care composition in the toner is 0.01-2%. More preferably, the mass concentration of the plant extract in the skin care composition in the toner is 0.50-1.25%.

[0069] More preferably, the mass concentration of the plant extract in the skin care composition in the toner is 1.05%.

[0070] The skin care composition is mixed with plant essence water and filtered to obtain toner.

[0071] It should be understood that products containing the skin care composition of the present invention, as well as products further added with auxiliary functional agents and / or plant additives, all fall within the scope of protection of the present invention. For example, the skin care composition of the present invention may also be presented in the form of emulsions, gels, sachets, lotions, oils, plasters, granules, sprays, creams, and / or combinations thereof.

[0072] The preparation method of the present invention can be carried out by conventional methods in the art or by methods disclosed in specific embodiments of the present invention.

[0073] Several specific embodiments of the present invention are given below, but the present invention is not limited by the embodiments.

[0074] In addition, if not otherwise stated, all raw materials used were commercially available.

[0075] The sources of the raw materials used in the present invention are shown in Table 1:

[0076] Table 1

[0077] raw material source chrysanthemum Shouxian Valley Organic Chinese Medicine Health Garden honeysuckle Anhui Yaozhiyuan Chinese Medicine Pieces Co., Ltd. Lavender Anhui Yaozhiyuan Chinese Medicine Pieces Co., Ltd. Peony Anhui Yaozhiyuan Chinese Medicine Pieces Co., Ltd. Dendrobium officinale powder (crude extract) Shouxian Valley Organic Chinese Medicine Health Garden Gynostemma pentaphyllum extract (gypenosides) Zhejiang Tiancao Biotechnology Co., Ltd.

[0078] The present invention has the following beneficial effects:

[0079] 1. The present invention utilizes condensed water formed during the plant extraction process to replace purified water, which can save purified water usage, reduce waste discharge, and fully tap the value of plant resources;

[0080] 2. The skin care composition and / or toner of the present invention has skin care effects such as moisturizing, anti-inflammatory, and anti-oxidation, has no side effects, and is highly safe;

[0081] 3. The skin care composition and / or toner of the present invention has at least one of the following effects: preventing and treating acne / pimples, refreshing skin, promoting cell division and improving cell survival rate, and moisturizing, and also has an antibacterial effect;

[0082] 4. The skin care composition and / or toner of the present invention has good penetration effect;

[0083] 5. The skin care composition and / or toner of the present invention has low foaming properties and can be transported well without affecting normal use. BRIEF DESCRIPTION OF THE DRAWINGS

[0084] Figure 1This is a negative control result diagram observed by fluorescence microscope in the ROS detection test of the present invention;

[0085] Figure 2 This is a model control result diagram observed by fluorescence microscope in the ROS detection experiment of the present invention;

[0086] Figure 3 This is a result diagram of the sample in Example 2 observed by fluorescence microscope in the ROS detection test of the present invention;

[0087] Figure 4 This is a result diagram of the sample in Example 1 observed by fluorescence microscope in the ROS detection test of the present invention;

[0088] Figure 5 Analysis of IL-1β content results. DETAILED DESCRIPTION

[0089] Example 1

[0090] Embodiment 1 of the present invention specifically provides a skin care composition containing plant essence water, wherein the skin care composition includes plant extracts and plant essence water.

[0091] The plant essence water is Ganoderma lucidum essence water;

[0092] Lingzhi essence water is a large amount of condensed water formed in the production process of Lingzhi extract. The preparation method is: take Lingzhi water extract, concentrate it by vacuum concentration, and collect the condensed water;

[0093] The plant extracts include chrysanthemum extract and honeysuckle extract;

[0094] The weight of the chrysanthemum extract is 0.5% by weight of the skin care composition, and the weight of the honeysuckle extract is 0.5% by weight of the skin care composition;

[0095] The plant extracts also include Gynostemma pentaphyllum extract;

[0096] The weight of the Gynostemma pentaphyllum extract is 0.5% by weight of the skin care composition;

[0097] The plant extracts also include a Dendrobium officinale extract, which accounts for 0.08% by weight of the skin care composition;

[0098] The preparation method of the chrysanthemum extract is as follows: (1) primary decolorization: soak the chrysanthemum in 10 times the volume of 95% ethanol overnight, filter, naturally dry, evaporate the ethanol, and set aside; (2) extract preparation: take the raw material after primary decolorization, use purified water for reflux extraction twice, the solid-liquid ratio is 15 times and 10 times respectively, each extraction is 1 hour, filter, combine the filtrate, and obtain the chrysanthemum extract; (3) decolorization: take the chrysanthemum extract, add activated carbon powder, dynamically adsorb and decolorize for more than 4 hours, filter through a 0.45 μL filter membrane, and obtain the chrysanthemum extract; (4) alcohol precipitation: take the chrysanthemum extract, add 95% ethanol in a volume ratio of 1:3 (stirring while adding), cover the lid, and let it stand at low temperature for alcohol precipitation overnight to obtain the chrysanthemum precipitate, and set aside; (5) drying: centrifuge the chrysanthemum precipitate at 4000 rpm for 10 minutes; remove the supernatant, and obtain the precipitate and dry it to obtain the chrysanthemum extract;

[0099] The preparation method of the honeysuckle extract is the same as that of the chrysanthemum extract;

[0100] The preparation method of the Dendrobium officinale extract is as follows: (1) dissolving: taking a crude extract of Dendrobium officinale, adding 100 times purified water and fully dissolving it at 80°C to obtain a Dendrobium officinale aqueous solution, which is set aside; (2) alcohol precipitation: taking a Dendrobium officinale aqueous solution, adding 95% ethanol at a volume ratio of 1:3 (adding while stirring), covering the lid, and standing at low temperature for alcohol precipitation overnight to obtain a Dendrobium officinale alcohol precipitation liquid, which is set aside; (3) drying: centrifuging the Dendrobium officinale alcohol precipitation liquid at 4000 rpm for 10 minutes; removing the supernatant, and drying the precipitate to obtain a Dendrobium officinale extract containing polysaccharides.

[0101] The preparation method of the skin care composition is as follows: (1) dividing the plant essence water into four groups of A, B, C and D, wherein the weight ratio of A, B, C and D is 5:5:1:1; (2) mixing the plant essence water of group A with chrysanthemum extract at 65°C to obtain A1, mixing the plant essence water of group B with honeysuckle extract at 65°C to obtain B1, mixing the plant essence water of group C with Dendrobium officinale extract at 65°C to obtain C1, and mixing the plant essence water of group D with Gynostemma pentaphyllum extract at 65°C to obtain D1; (3) evenly mixing A1, B1, C1 and D1 to obtain a skin care composition containing the plant essence water.

[0102] This embodiment also provides a toner, which comprises the skin care composition containing plant essence water and plant essence water-1;

[0103] The plant essence water-1 is the same as the plant essence water;

[0104] The mass concentration of the plant extract in the skin care composition in the toner is 0.5%;

[0105] The skin care composition is mixed with plant essence water and filtered to obtain toner.

[0106] Example 2

[0107] Embodiment 2 of the present invention specifically provides a skin care composition comprising plant essence water, wherein the skin care composition comprises a plant extract and plant essence water;

[0108] The plant essence water is Ganoderma lucidum essence water;

[0109] The Ganoderma lucidum essence water is the same as that in Example 1;

[0110] The plant extracts include chrysanthemum extract and honeysuckle extract;

[0111] The weight of the chrysanthemum extract is 0.8% by weight of the skin care composition, and the weight of the honeysuckle extract is 0.4% by weight of the skin care composition;

[0112] The plant extracts also include Gynostemma pentaphyllum extract;

[0113] The weight of the Gynostemma pentaphyllum extract is 0.3% by weight of the skin care composition;

[0114] The plant extracts also include a Dendrobium officinale extract, which accounts for 0.15 wt % of the skin care composition;

[0115] The preparation method of the chrysanthemum extract is the same as that of Example 1;

[0116] The preparation method of the honeysuckle extract is the same as that of the chrysanthemum extract;

[0117] The Dendrobium officinale extract is the same as that in Example 1;

[0118] The preparation method of the skin care composition is as follows: (1) dividing the plant essence water into four groups of A, B, C and D, wherein the weight ratio of A, B, C and D is 5:5:1:1; (2) mixing the plant essence water of group A with chrysanthemum extract at 90°C to obtain A1, mixing the plant essence water of group B with honeysuckle extract at 90°C to obtain B1, mixing the plant essence water of group C with Dendrobium officinale extract at 90°C to obtain C1, and mixing the plant essence water of group D with Gynostemma pentaphyllum extract at 90°C to obtain D1; (3) evenly mixing A1, B1, C1 and D1 to obtain a skin care composition containing the plant essence water.

[0119] This embodiment also provides a toner, which comprises the skin care composition containing plant essence water and plant essence water-1;

[0120] The plant essence water-1 is the same as the plant essence water;

[0121] The mass concentration of the plant extract in the skin care composition in the toner is 0.3%;

[0122] The skin care composition is mixed with plant essence water and filtered to obtain toner.

[0123] Example 3

[0124] Embodiment 3 of the present invention specifically provides a skin care composition comprising plant essence water, wherein the skin care composition comprises a plant extract and plant essence water;

[0125] The plant essence water is Ganoderma lucidum essence water;

[0126] The Ganoderma lucidum essence water is the same as that in Example 1;

[0127] The plant extracts include chrysanthemum extract and honeysuckle extract;

[0128] The weight of the chrysanthemum extract is 0.8% by weight of the skin care composition, and the weight of the honeysuckle extract is 0.8% by weight of the skin care composition;

[0129] The plant extracts also include Gynostemma pentaphyllum extract;

[0130] The weight of the Gynostemma pentaphyllum extract is 0.3% by weight of the skin care composition;

[0131] The plant extract also includes a Dendrobium officinale extract, which accounts for 0.1 wt % of the skin care composition;

[0132] The preparation method of the chrysanthemum extract is the same as that of Example 1;

[0133] The preparation method of the honeysuckle extract is the same as that of the chrysanthemum extract;

[0134] The Dendrobium officinale extract is the same as that in Example 1;

[0135] The preparation method of the skin care composition is as follows: (1) dividing the plant essence water into four groups of A, B, C and D, wherein the weight ratio of A, B, C and D is 5:5:1:1; (2) mixing the plant essence water of group A with chrysanthemum extract at 80°C to obtain A1, mixing the plant essence water of group B with honeysuckle extract at 80°C to obtain B1, mixing the plant essence water of group C with Dendrobium officinale extract at 80°C to obtain C1, and mixing the plant essence water of group D with Gynostemma pentaphyllum extract at 80°C to obtain D1; (3) evenly mixing A1, B1, C1 and D1 to obtain a skin care composition containing the plant essence water.

[0136] This embodiment also provides a toner, which comprises the skin care composition containing plant essence water and plant essence water-1;

[0137] The plant essence water-1 is the same as the plant essence water;

[0138] The mass concentration of the plant extract in the skin care composition in the toner is 1.25%; the toner is prepared by mixing the skin care composition with the plant essence water and filtering.

[0139] Example 4

[0140] Example 4 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that lavender extract is used instead of honeysuckle extract.

[0141] Example 5

[0142] Example 5 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that peony extract is used instead of chrysanthemum extract.

[0143] Example 6

[0144] Example 6 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that there is no chrysanthemum extract.

[0145] Example 7

[0146] Example 7 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that there is no honeysuckle extract.

[0147] Example 8

[0148] Example 8 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that there is no Gynostemma pentaphyllum extract.

[0149] Example 9

[0150] Example 9 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that there is no Dendrobium officinale extract.

[0151] Example 10

[0152] Example 10 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that the Dendrobium officinale extract accounts for 0.03wt% of the skin care composition.

[0153] Embodiment 11

[0154] Example 11 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that the Dendrobium officinale extract accounts for 0.25wt% of the skin care composition.

[0155] Example 12

[0156] Example 12 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that the mass concentration of the plant extract in the skin care composition in the toner is 0.16%.

[0157] Embodiment 13

[0158] Example 13 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that the mass concentration of the plant extract in the skin care composition in the toner is 0.31%.

[0159] Embodiment 14

[0160] Example 14 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3, except that the mass concentration of the plant extract in the skin care composition in the toner is 0.63%.

[0161] Embodiment 15

[0162] Example 15 of the present invention specifically provides a skin care composition comprising plant essence water and a toner, and its specific implementation method is the same as that of Example 3. The mass concentration of the plant extract in the skin care composition in the toner is 0.08%.

[0163] Example 16 Performance Test

[0164] The toner in Examples 1-15 complies with the standard QB / T2660-2004, has a uniform liquid appearance, is clear and transparent, and is not turbid and has no precipitation after being left for 30 days.

[0165] (1) Refreshing feeling test: The efficacy of the toners in some embodiments was evaluated by trial feeling. A public opinion survey scoring method was adopted. 30 women aged 25-55 were selected as trial subjects. On the first day, a toner in one embodiment was used. On the second day, a toner in another embodiment was used. The toner was sprayed on the face as evenly as possible twice. The test was conducted in this order. The total score of the use effect was 5 points: 5 points was the highest score, indicating very good, very satisfactory; 4 points was good; 3 points was acceptable; and less than 3 points was not good, unacceptable. The test results are shown in Table 2.

[0166] (2) Acne removal evaluation: 240 volunteers with facial acne problems, aged 18-28 years old, were selected and divided into 8 groups, 30 people in each group used the toner of Examples 1-8, once a day in the morning and once in the evening, for 2 consecutive months. Evaluation criteria: Grade A, facial oil secretion is significantly reduced, and acne is basically eliminated; Grade B, facial oil secretion is reduced, and some acne is eliminated; Grade C, acne has not been reduced or the reduction is not obvious. The test results are shown in Table 2.

[0167] (3) Foam height test: The same amount of toner in some embodiments is placed in the same sealed container, stirred at the same speed for 5 minutes, and the height of the foam in the container is observed. Evaluation criteria: Grade A, foam height is less than or equal to 3 mm, Grade B, foam height is greater than 3 mm and less than or equal to 8 mm; Grade C, foam height is less than 8 mm and greater than or equal to 12 mm; Grade D, foam height is greater than 12 mm. The test results are shown in Table 2.

[0168] (4) Cell viability test:

[0169] Test sample: 10% of the toner in some embodiments was added to serum-free DMEM medium to prepare a sample;

[0170] Test method: Collect cells in logarithmic growth phase, inoculate into 96-well plates at a cell density of 8000 cells / well, and each well contains 100μL of culture medium. After culturing in an incubator (37°C, 5% CO2) for 24 hours, remove the culture medium, wash the cells twice with PBS buffer, and then add samples, 100μL per well, with untreated cells as negative control (NC), and set up 3 replicate wells for each group. After adding samples, continue to culture in an incubator (37°C, 5% CO2) for 24 hours, and then perform CCK-8 cytotoxicity test, read the absorbance OD value at 450nm, and calculate the cell survival rate according to the following formula. Only cell culture medium is added to the blank well:

[0171] (OD of the dosing well-OD of the blank well) / (OD of the control well-OD of the blank well)×100%=cell survival rate, and the test results are shown in Table 2.

[0172] (5) ROS detection test:

[0173] Test samples: The toners in Examples 2-3 were added to DMEM medium at 10% to prepare samples;

[0174] Test method: Collect cells in logarithmic growth phase and 5Cells / well were seeded in 6-well plates. After culturing in an incubator (37°C, 5% CO2) for 24 hours, samples were added. After adding samples, the cells were cultured in an incubator (37°C, 5% CO2) for another 24 hours. The culture medium was removed and the cells were gently washed twice with 1 mL of pre-warmed PBS. The washing solution was removed and 1 mL of PBS was added. 2 The cells were illuminated at a dose of 50 μg / mL. After reaching the illumination dose, the PBS in the wells was removed, and the wells were gently washed once with 1 ml of pre-warmed PBS. DMEM culture medium was added and cultured for 16-24 hours. Untreated cells were used as negative controls, and cells that were only illuminated without adding drugs were used as model controls. The DCFH-DA probe mother solution was diluted with serum-free culture medium at a ratio of 1:1000 to a final concentration of 10 μmol / L. 500 μL of the diluted DCFH-DA probe was added to each well and incubated in a cell culture incubator at 37°C. After 20 minutes, the cells were washed 4 times with serum-free DMEM culture medium to fully remove the probes that did not enter the cells. The cells were observed and photographed using a fluorescence microscope at an excitation wavelength of 488 nm. The results are shown in the figure. Figure 1-4 shown.

[0175] (6) Moisture retention test: Cells in the logarithmic growth phase were collected and inoculated into 6-well plates at a cell density of 3×105 cells / well. After culturing in an incubator (37° C., 5% CO2) for 24 h, the toners in Example 1 and Example 2 were added according to the cytotoxicity results, 50 μg / ml of Ural Glycyrrhiza uralensis extract was used as a positive control, untreated cells were used as a negative control, and KC Growth medium containing 10% Ganoderma lucidum essence water was used as a solvent control, with 3 replicate wells set for each group.

[0176] After adding the drug, the cells were cultured in an incubator (37°C, 5% CO2) for 48 h. RNA was extracted using the Mini Kit and then One Step TB PrimeScript TM Real time-qPCR reaction was performed using RT-PCR Kit II and the Ct value was determined. β-actin was used as the internal reference gene to calculate the relative expression folds of aquaporin 3 (AQP3) and filaggrin (FLG) in KC cells after sample treatment compared with those in the solvent control group.

[0177] AQP3 or FLG relative expression fold = 2 -(Ct AQP3或FLG,样品组-Ctβ-actin,样品组-Ct AQP3或FLG,对照组+Ctβ-actin,对照组) .

[0178] The test results are shown in Table 3 and Table 4. Table 3 is the analysis of the relative expression multiple of AQP3; Table 4 is the analysis of the relative expression multiple of FLG.

[0179] (7) Anti-inflammatory test

[0180] The experiment was divided into 4 groups: negative control group (NC); LPS model group: no sample pretreatment, only LPS stimulation; sample group: sample pretreatment followed by LPS stimulation; positive control group (PC): 0.01 mg / mL dexamethasone pretreatment followed by LPS stimulation.

[0181] Cells in the logarithmic growth phase were collected and the cell density was 4×10 5 Each well was inoculated into a 12-well plate. After culturing in an incubator (37°C, 5% CO2) for 24 hours, a concentration gradient was set according to the customer's recommended concentration, and the toners of Examples 1-2 were added respectively. After culturing in an incubator (37°C, 5% CO2) for 2 hours, 1 μg / mL LPS was added to the model group, sample group, and positive control group, and the cells were placed in a cell incubator for 24 hours. The mouse IL-1β ELISA kit was used to detect the IL-1β content in the culture medium according to the instructions.

[0182] The test results are shown in Table 5 and Figure 5 As shown, Table 4 is the analysis of IL-1β content results; Figure 5 Analysis of IL-1 content results.

[0183] Table 2

[0184]

[0185] It can be seen from the test results in Table 1 that the skin care composition and toner containing plant essence water provided by the present invention can effectively prevent and treat acne and pimples, have good permeability and refreshing skin feel, and can also improve the survival rate of cells.

[0186] Table 3

[0187] AQP3 relative expression SD Solvent Control 1 0.02 Positive Control 1.32 0.03 Example 1 1.59 0.05 Example 2 1.34 0.03

[0188] Table 4

[0189] AQP3 relative expression SD Solvent Control 1 0.04 Positive Control 9.44 0.31 Example 1 2.22 0.11 Example 2 1.25 0.02

[0190] The results in Example 1 and Example 2 show that the skin care composition and toner thereof of the present invention can enhance the expression levels of AQP3 and FLG in KC cells and have a certain moisturizing effect.

[0191] Table 5

[0192] IL-1β content (pg / ml) SD Negative control (NC) 1.17 0.67 LPS Model 406.09 47.56 Positive Control (PC) 51.59 21.50 Example 1 78.41 10.11 Example 2 59.73 7.79

[0193] From Table 5 and Figure 5The test results show that compared with the negative control group, the secretion level of the inflammatory factor IL-1β in the LPS model group was significantly increased, indicating that the model was successfully established and the model can be used to evaluate the anti-inflammatory and soothing effects of the test substance. Compared with the LPS model group, the IL-1β content decreased after treating RAW264.7 cells in Example 1-2. This shows that the toner of the present invention can reduce the release of the inflammatory factor IL-1β.

[0194] And by Figure 1-4 It can be seen that compared with the negative control result graph, at 10J / cm 2 After irradiation at the dose, obvious green fluorescence appeared in the cells of the model group, indicating that the model was successful; compared with the model control, when the cells were acted on by the samples in Example 2 and Example 3, the green fluorescence in the cells was enhanced, indicating that the samples could not reduce the level of ROS, that is, the samples could promote cell division and improve cell survival rate.

[0195] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to claim the widest possible range that can be conceived, and the embodiments presented herein are only illustrations of selected implementations according to the combination of all possible embodiments. Therefore, it is the applicant's intention that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges within them, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. A skin care composition comprising plant essence water, characterized in that: The skin care composition comprises plant extracts and plant essence water; The plant essence water is Ganoderma lucidum essence water; The plant extracts are composed of chrysanthemum extract, honeysuckle extract, gynostemma pentaphyllum extract and dendrobium officinale extract; The weight of the chrysanthemum extract is 0.05%-2% of the weight of the skin care composition; The weight of the honeysuckle extract is 0.05%-2% of the weight of the skin care composition; the weight of the Gynostemma pentaphyllum extract is 0.05%-1% of the weight of the skin care composition; The Dendrobium officinale extract accounts for 0.05-1% by weight of the skin care composition; The main active ingredients of the chrysanthemum extract, honeysuckle extract and dendrobium officinale extract are polysaccharides, and the main active ingredient of the gynostemma pentaphyllum extract is saponin; The ganoderma essence water is a large amount of condensed water formed during the production process of ganoderma extract.

2. The skin care composition comprising plant essence water according to claim 1, characterized in that: The Gynostemma pentaphyllum extract is gypenosin, and the weight of the gypenosin is 10%-50% of the weight of the plant extract.

3. A toner, characterized in that: The toner comprises the skin care composition comprising plant essence water according to claim 1.

4. The toner according to claim 3, characterized in that: The mass concentration of the plant extract in the skin care composition in the toner is 0.01-2%.

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