Preparation method and application of a firming and anti-aging composition

Abstract

The present invention relates to a preparation method and application of a firming and anti-aging composition, and belongs to the technical field of skin care products. The composition comprises components: fermented product of pyracantha fruit, roxburghii extract, malachite extract, recombinant type III humanized collagen and adjuvant, wherein the adjuvant is composed of menthol and oligosphingosine. In the fermentation and extraction of pyracantha fruit powder and pyracantha fruit extract, a large amount of small molecule polypeptides and low molecular weight polysaccharides are generated, and the small molecule polypeptides and low molecular weight polysaccharides are more easily absorbed by the skin, and synergistically act with recombinant type III humanized collagen to improve the inhibitory effect of the composition on elastase, thereby improving the firming and anti-aging effects of the resident skin topical preparation.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products and relates to a preparation method of a firming and anti-aging composition and application thereof. Background Art

[0002] As the pace of life in modern society accelerates, people are facing more and more pressure. Environmental pollution, unreasonable diet, lack of sleep and other factors may have a negative impact on human health, especially skin problems. As the largest organ of the human body, the health of the skin directly reflects a person's overall health level. However, due to aging, ultraviolet radiation, free radical damage and other reasons, the skin will gradually lose its elasticity and luster, and wrinkles, sagging and other aging phenomena will appear. Therefore, how to effectively delay skin aging and keep the skin firm has always been a hot topic in the field of skin care research. A large number of studies have shown that changes in the biological properties of elastic fibers (including reduction in quantity, morphological changes, weakened or declining functions, etc.) play an important role in the formation of wrinkles. Therefore, the quantity, quality and activity of elastic fibers directly determine the degree of skin aging.

[0003] There are many anti-aging products on the market, which usually contain various antioxidants, collagen, hyaluronic acid and other ingredients, aiming to achieve anti-aging effects by replenishing the nutrients needed by the skin, improving skin elasticity and moisture content, etc. However, these products often have some problems, such as short-term effects, frequent use, high prices, etc., which cannot meet the needs of the majority of consumers.

[0004] In summary, in view of the problems and shortcomings of the prior art, the present invention provides a firming and anti-aging composition, which can improve skin condition, delay skin aging process, and has significant market potential and application value. Summary of the invention

[0005] The object of the present invention is to provide a method for preparing a firming and anti-aging composition and its application, which has the characteristics of being easy to absorb and having good firming and anti-aging effects.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A firming and anti-aging composition, comprising the following components in parts by mass:

[0008] 1-5 servings of Pyracantha fruit fermentation

[0009] 0.01-0.05 parts of roxburghii extract

[0010] Malachite extract 0.5-3 parts

[0011] Recombinant humanized type III collagen 2-6 copies

[0012] Auxiliary agent 0.1-0.5 parts.

[0013] As a preferred technical solution of the present invention, the following components are included in parts by mass:

[0014] 2-4 servings of fermented pyracantha fruit

[0015] 0.02-0.04 parts of roxburghii extract

[0016] Malachite extract 1-2 parts

[0017] 3-5 copies of recombinant humanized type III collagen

[0018] Auxiliary agent 0.2-0.4 parts.

[0019] As a preferred technical solution of the present invention, the Pyracantha fortuneana fruit fermentation product is prepared by the following steps, calculated by weight:

[0020] W1. 8-12 parts of Pyracantha fruit washed with water and dried and mixed, ground into Pyracantha fruit powder, mixed with 4-8 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50-60% by volume, autoclaved at 110-120 ° C for 20-40min to obtain a sterilized wet mixed powder;

[0021] W2. Sprinkle 200-300 mL of Bacillus subtilis suspension evenly on 1500-2000 g of sterilized wet mixed powder, adjust the initial pH to 6-8, culture at 34-38°C for 40-48 hours, then sterilize, cool, filter and remove residue, centrifuge at 4000-4200 r / min, filter with a filter membrane, and obtain a filtrate, which is the fermentation product of Pyracantha fruit.

[0022] As a preferred technical solution of the present invention, the cell concentration of the Bacillus subtilis suspension is 1×10 8 -5×10 8 CFU / mL.

[0023] As a preferred technical solution of the present invention, the adjuvant is composed of 1-5 parts of menthol and 4-8 parts of oligosphingosine.

[0024] As a preferred technical solution of the present invention, the auxiliary agent consists of 2-3 parts of menthol and 5-7 parts of oligosphingosine.

[0025] As a preferred technical solution of the present invention, the firming and anti-aging composition is applied to a leave-on skin topical preparation.

[0026] As a preferred technical solution of the present invention, the addition ratio of the firming and anti-aging composition in the resident skin topical preparation is ≤10wt%.

[0027] As a preferred technical solution of the present invention, the addition ratio of the firming and anti-aging composition in the resident skin topical preparation is ≤5wt%.

[0028] As a preferred technical solution of the present invention, the dosage form of the leave-on skin topical preparation is emulsion, gel or cream.

[0029] Beneficial effects of the present invention:

[0030] (1) The present invention uses Bacillus subtilis to ferment Pyracantha fortuneana fruit powder and Pyracantha fortuneana fruit extract mixed in a specific ratio to obtain Pyracantha fortuneana fruit fermentation product, and the Pyracantha fortuneana fruit fermentation product and recombinant humanized type III collagen act synergistically to improve the firming and anti-aging effect of the firming and anti-aging composition.

[0031] (2) Pyracantha fruit contains rich biological polypeptides, and the main component of Pyracantha fruit extract is polysaccharide. When the present invention uses Bacillus subtilis for fermentation, on the one hand, the growth activity of Bacillus subtilis decomposes and consumes the Pyracantha fruit extract, generating organic matter such as organic acids and polypeptides; on the other hand, Bacillus subtilis secretes a variety of biological enzymes during the fermentation process. These enzymes can not only accelerate the destruction of the cell wall of Pyracantha fruit powder and release intracellular active substances, degrade potential harmful substances through biotransformation, and generate beneficial secondary metabolites, but also promote the hydrolysis of polypeptides, generating a large number of small molecule polypeptides, and the fermentation process can also generate organic acids, which can further promote the release and extraction of polypeptides.

[0032] (3) In the fermentation and extraction of Pyracantha fortuneana fruit powder and Pyracantha fortuneana fruit extract of the present invention, the metabolic activity of Bacillus subtilis causes the Pyracantha fortuneana fruit fermentation product to contain rich small molecule polypeptides and low molecular weight polysaccharides. The small molecule polypeptides and low molecular weight polysaccharides are more easily absorbed by the skin, thereby improving the firming and anti-aging effects of the firming and anti-aging composition. DETAILED DESCRIPTION

[0033] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0034] The specific sources of some ingredients of the present invention are as follows:

[0035] Pyracantha fruit: Pyracantha (PYRACANTHA FORTUNEANA) Xi'an Anao Biotechnology Co., Ltd.

[0036] Pyracantha Fruit Extract: Pyracantha Fortuneana Xi'an Anao Biotechnology Co., Ltd.

[0037] Rosa Roxburi Extract: Rosa Roxburi (ROSA ROXBURGHII) Xi'an Anao Biotechnology Co., Ltd.

[0038] Malachite Extract: Malachite (MALACHITE) Xi'an Anao Biotechnology Co., Ltd.

[0039] Recombinant humanized type III collagen: Jiangsu Chuangjian Medical Technology Co., Ltd.

[0040] Oligosphingosine: Yuanpeptide Biotechnology (Tianjin) Co., Ltd.

[0041] Example 1

[0042] A firming and anti-aging composition, comprising the following components in parts by mass:

[0043] 1 serving of Pyracantha fruit fermentation

[0044] 0.01 parts of roxburghii extract

[0045] Malachite extract 0.5 parts

[0046] 2 copies of recombinant humanized type III collagen

[0047] 0.1 part of auxiliary agent.

[0048] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0049] W1. 8 parts of Pyracantha fruit washed with water and dried and then mixed, ground into Pyracantha fruit powder, mixed with 4 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and autoclaved at 110 ° C for 20 min to obtain a sterilized wet mixed powder;

[0050] W2. Sprinkle 200 mL of Bacillus subtilis suspension evenly on 1500 g of sterilized wet mixed powder, adjust the initial pH to 6, culture at 34°C for 40 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4000 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0051] The cell concentration of the Bacillus subtilis suspension is 1×10 8 CFU / mL.

[0052] The auxiliary agent consists of 1 part of menthol and 4 parts of oligosphingosine.

[0053] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0054] The addition ratio of the firming and anti-aging composition is 2wt%.

[0055] The dosage form of the leave-on skin external preparation is an emulsion.

[0056] Example 2

[0057] A firming and anti-aging composition, comprising the following components in parts by mass:

[0058] 2 servings of Pyracantha fruit fermentation

[0059] 0.02 parts of roxburghii extract

[0060] 1 part Malachite extract

[0061] Recombinant humanized type III collagen 3 copies

[0062] 0.2 parts of auxiliary agent.

[0063] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0064] W1. 9 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 5 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and sterilized at 112 ° C for 25 min to obtain a sterilized wet mixed powder;

[0065] W2. Sprinkle 220 mL of Bacillus subtilis suspension evenly on 1500 g of sterilized wet mixed powder, adjust the initial pH to 6, culture at 35°C for 42 hours, then sterilize at 120°C, cool at 4°C for 5 minutes, filter through a 30μm filter membrane to remove residue, centrifuge at 4000r / min, and filter through a 20μm filter membrane to obtain a filtrate, which is the fermentation product of Pyracantha fruit.

[0066] The cell concentration of the Bacillus subtilis suspension is 2×10 8 CFU / mL.

[0067] The auxiliary agent consists of 2 parts of menthol and 5 parts of oligosphingosine.

[0068] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0069] The addition ratio of the firming and anti-aging composition is 4wt%.

[0070] The dosage form of the leave-on skin external preparation is emulsion, gel or cream.

[0071] Example 3

[0072] A firming and anti-aging composition, comprising the following components in parts by mass:

[0073] 3 servings of Pyracantha fruit fermentation

[0074] 0.03 parts of roxburghii extract

[0075] Malachite extract 1.5 parts

[0076] 4 copies of recombinant humanized type III collagen

[0077] 0.3 parts of auxiliary agent.

[0078] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0079] W1. 10 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 6 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and autoclaved at 115 ° C for 30 min to obtain a sterilized wet mixed powder;

[0080] W2. Sprinkle 250 mL of Bacillus subtilis suspension evenly on 1750 g of sterilized wet mixed powder, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0081] The cell concentration of the Bacillus subtilis suspension is 3×10 8 CFU / mL.

[0082] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0083] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0084] The addition ratio of the firming and anti-aging composition is 6wt%.

[0085] The dosage form of the leave-on skin external preparation is a gel.

[0086] Example 4

[0087] A firming and anti-aging composition, comprising the following components in parts by mass:

[0088] 4 servings of Pyracantha fruit fermentation

[0089] 0.04 parts of roxburghii extract

[0090] 2 parts of Malachite Extract

[0091] Recombinant humanized type III collagen 5 copies

[0092] 0.4 parts of auxiliary agent.

[0093] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0094] W1. 11 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 7 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 60% by volume, and sterilized at 117 ° C for 35 min to obtain a sterilized wet mixed powder;

[0095] W2. Sprinkle 270 mL of Bacillus subtilis suspension evenly on 2000 g of sterilized wet mixed powder, adjust the initial pH to 8, culture at 37°C for 46 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4200 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0096] The cell concentration of the Bacillus subtilis suspension is 4×10 8 CFU / mL.

[0097] The auxiliary agent consists of 4 parts of menthol and 7 parts of oligosphingosine.

[0098] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0099] The addition ratio of the firming and anti-aging composition is 8wt%.

[0100] The dosage form of the leave-on skin external preparation is a cream.

[0101] Example 5

[0102] A firming and anti-aging composition, comprising the following components in parts by mass:

[0103] 5 servings of Pyracantha fruit fermentation

[0104] 0.05 parts of roxburghii extract

[0105] 3 parts of Malachite Extract

[0106] Recombinant humanized type III collagen 6 copies

[0107] 0.5 parts of auxiliary agent.

[0108] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0109] W1. 12 parts of Pyracantha fruit washed with water and dried and then mixed, ground into Pyracantha fruit powder, mixed with 8 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 60% by volume, and autoclaved at 120 ° C for 40 min to obtain a sterilized wet mixed powder;

[0110] W2. Sprinkle 300 mL of Bacillus subtilis suspension evenly on 2000 g of sterilized wet mixed powder, adjust the initial pH to 8, culture at 38°C for 48 hours, then sterilize at 120°C, cool at 4°C for 5 minutes, filter through a 30μm filter membrane to remove residue, centrifuge at 4200r / min, and filter through a 20μm filter membrane to obtain the filtrate, which is the fermented product of Pyracantha fruit.

[0111] The cell concentration of the Bacillus subtilis suspension is 5×10 8 CFU / mL.

[0112] The auxiliary agent consists of 5 parts of menthol and 8 parts of oligosphingosine.

[0113] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0114] The addition ratio of the firming and anti-aging composition is 10wt%.

[0115] The dosage form of the leave-on skin external preparation is a cream.

[0116] Comparative Example 1

[0117] A firming and anti-aging composition, comprising the following components in parts by mass:

[0118] 0.03 parts of roxburghii extract

[0119] Malachite extract 1.5 parts

[0120] Recombinant humanized type III collagen 7 parts

[0121] 0.3 parts of auxiliary agent.

[0122] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0123] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0124] The addition ratio of the firming and anti-aging composition is 6wt%.

[0125] The dosage form of the leave-on skin external preparation is a gel.

[0126] Comparative Example 2

[0127] A firming and anti-aging composition, comprising the following components in parts by mass:

[0128] 3 servings of fermented pyracantha fruit powder

[0129] 0.03 parts of roxburghii extract

[0130] Malachite extract 1.5 parts

[0131] 4 copies of recombinant humanized type III collagen

[0132] 0.3 parts of auxiliary agent.

[0133] Calculated by weight, the fermented pyracantha fruit powder is prepared by the following steps:

[0134] W1. 16 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, moistened with distilled water, maintaining a water content of 50% by volume, and sterilized at 115 ° C for 30 min to obtain sterilized wet Pyracantha fruit powder;

[0135] W2. Sprinkle 250 mL of Bacillus subtilis suspension evenly on 1750 g of sterilized wet Pyracantha fortuneana fruit powder, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermentation product of Pyracantha fortuneana fruit powder.

[0136] The cell concentration of the Bacillus subtilis suspension is 3×10 8 CFU / mL.

[0137] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0138] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0139] The addition ratio of the firming and anti-aging composition is 6wt%.

[0140] The dosage form of the leave-on skin external preparation is a gel.

[0141] Comparative Example 3

[0142] A firming and anti-aging composition, comprising the following components in parts by mass:

[0143] 3 servings of Pyracantha fortuneana fruit extract fermentation

[0144] 0.03 parts of roxburghii extract

[0145] Malachite extract 1.5 parts

[0146] 4 copies of recombinant humanized type III collagen

[0147] 0.3 parts of auxiliary agent.

[0148] Calculated by weight, the fermented product of Pyracantha fortuneana fruit extract is prepared by the following steps:

[0149] W1. Take 16 parts of Pyracantha fruit extract, moisten with distilled water, maintain a water content of 50% by volume, and sterilize at 115°C for 30 minutes to obtain a sterilized moist Pyracantha fruit extract;

[0150] W2. Sprinkle 250 mL of Bacillus subtilis suspension evenly on 1750 g of sterilized wet Pyracantha fortuneana fruit extract, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermentation product of Pyracantha fortuneana fruit extract.

[0151] The cell concentration of the Bacillus subtilis suspension is 3×10 8 CFU / mL.

[0152] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0153] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0154] The addition ratio of the firming and anti-aging composition is 6wt%.

[0155] The dosage form of the leave-on skin external preparation is a gel.

[0156] Comparative Example 4

[0157] A firming and anti-aging composition, comprising the following components in parts by mass:

[0158] 3 parts of Pyracantha fortuneana fruit extract

[0159] 0.03 parts of roxburghii extract

[0160] Malachite extract 1.5 parts

[0161] 4 copies of recombinant humanized type III collagen

[0162] 0.3 parts of auxiliary agent.

[0163] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0164] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0165] The addition ratio of the firming and anti-aging composition is 6wt%.

[0166] The dosage form of the leave-on skin external preparation is a gel.

[0167] Comparative Example 5

[0168] A firming and anti-aging composition, comprising the following components in parts by mass:

[0169] 7 servings of Pyracantha fruit fermentation

[0170] 0.03 parts of roxburghii extract

[0171] Malachite extract 1.5 parts

[0172] 0.3 parts of auxiliary agent.

[0173] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0174] W1. 10 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 6 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and autoclaved at 115 ° C for 30 min to obtain a sterilized wet mixed powder;

[0175] W2. Sprinkle 250 mL of Bacillus subtilis suspension evenly on 1750 g of sterilized wet mixed powder, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0176] The cell concentration of the Bacillus subtilis suspension is 3×10 8 CFU / mL.

[0177] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0178] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0179] The addition ratio of the firming and anti-aging composition is 6wt%.

[0180] The dosage form of the leave-on skin external preparation is a gel.

[0181] Comparative Example 6

[0182] A firming and anti-aging composition, comprising the following components in parts by mass:

[0183] 3 servings of Pyracantha fruit fermentation

[0184] 0.03 parts of roxburghii extract

[0185] Malachite extract 1.5 parts

[0186] 4 copies of recombinant humanized type III collagen

[0187] 0.3 parts of auxiliary agent.

[0188] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0189] W1. 10 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 6 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and autoclaved at 115 ° C for 30 min to obtain a sterilized wet mixed powder;

[0190] W2. Sprinkle 250 mL of Aspergillus oryzae suspension evenly on 1750 g of sterilized wet mixed powder, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0191] The cell concentration of the Aspergillus oryzae suspension is 3×10 8 CFU / mL.

[0192] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0193] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0194] The addition ratio of the firming and anti-aging composition is 6wt%.

[0195] The dosage form of the leave-on skin external preparation is a gel.

[0196] Comparative Example 7

[0197] A firming and anti-aging composition, comprising the following components in parts by mass:

[0198] 3 servings of Pyracantha fruit fermentation

[0199] 0.03 parts of roxburghii extract

[0200] Malachite extract 1.5 parts

[0201] 4 copies of recombinant humanized type III collagen

[0202] 0.3 parts of auxiliary agent.

[0203] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0204] W1. 14 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 10 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and sterilized at 115 ° C for 30 min to obtain a sterilized wet mixed powder;

[0205] W2. Sprinkle 250 mL of Bacillus subtilis suspension evenly on 1750 g of sterilized wet mixed powder, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0206] The cell concentration of the Bacillus subtilis suspension is 3×10 8 CFU / mL.

[0207] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0208] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0209] The addition ratio of the firming and anti-aging composition is 6wt%.

[0210] The dosage form of the leave-on skin external preparation is a gel.

[0211] Comparative Example 8

[0212] A firming and anti-aging composition, comprising the following components in parts by mass:

[0213] 6 servings of Pyracantha fruit fermentation

[0214] 0.03 parts of roxburghii extract

[0215] Malachite extract 1.5 parts

[0216] Recombinant humanized type III collagen 7 parts

[0217] 0.3 parts of auxiliary agent.

[0218] Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps:

[0219] W1. 10 parts of Pyracantha fruit were washed with water, dried and mixed, ground into Pyracantha fruit powder, mixed with 6 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50% by volume, and autoclaved at 115 ° C for 30 min to obtain a sterilized wet mixed powder;

[0220] W2. Sprinkle 250 mL of Bacillus subtilis suspension evenly on 1750 g of sterilized wet mixed powder, adjust the initial pH to 7, culture at 36°C for 44 h, then sterilize at 120°C, cool at 4°C for 5 min, filter through a 30 μm filter membrane to remove residue, centrifuge at 4100 r / min, and filter through a 20 μm filter membrane to obtain a filtrate, which is the fermented product of Pyracantha fruit.

[0221] The cell concentration of the Bacillus subtilis suspension is 3×10 8 CFU / mL.

[0222] The auxiliary agent consists of 3 parts of menthol and 6 parts of oligosphingosine.

[0223] A leave-on skin external preparation comprises the above-mentioned firming and anti-aging composition.

[0224] The addition ratio of the firming and anti-aging composition is 6wt%.

[0225] The dosage form of the leave-on skin external preparation is a gel.

[0226] Performance Testing

[0227] The firming and anti-aging compositions obtained in Examples 1-5 and Comparative Examples 1-8 were used as test objects and the following tests were performed:

[0228] 1. Anti-skin aging test of firming and anti-aging composition

[0229] Firming and anti-aging detection method: Skin sagging and aging are mainly related to elastic fibers, which are composed of elastin and microfibrils. Elastic fibers will be decomposed by elastase, causing collagen loss and thus leading to skin sagging and aging. Therefore, the purpose of firming and anti-aging effect detection can be achieved by measuring the inhibition rate of elastase by the firming and anti-aging composition.

[0230] The specific operation is to mix 100 μL of 0.2 mol / L tris-HCl buffer, 25 μL of 10 mmol / L N-methoxysuccinyl-alanine-proline-valine-4-nitroaniline and 30 mg of the firming and anti-aging composition, let it stand at 25°C for 10 minutes, add 25 μL of 0.3 U / mL elastase and continue to stand for 10 minutes, and finally detect the OD value at 410 nm, and use 0.3 mL of deionized water instead of the firming and anti-aging composition as a blank control. The results are recorded in Table 1 below.

[0231] The inhibition rate of the firming and anti-aging composition on elastase (%) = (OD value of the control group - OD value of the experimental group) / OD value of the control group × 100%.

[0232] Table 1 Performance test results

[0233]

[0234] The test object of comparative example 1 is a firming and anti-aging composition prepared without adding the fermented product of Pyracantha fortuneana fruit;

[0235] The test object of Comparative Example 2 is a firming and anti-aging composition prepared by fermenting only Pyracantha fortuneana fruit powder;

[0236] The test object of Comparative Example 3 is a firming and anti-aging composition prepared by fermentation using only the Pyracantha fortuneana fruit extract;

[0237] The test object of Comparative Example 4 is a firming and anti-aging composition prepared by using Pyracantha fortuneana fruit extract instead of Pyracantha fortuneana fruit fermentation product;

[0238] The test object of comparative example 5 is a firming and anti-aging composition prepared without adding recombinant humanized type III collagen;

[0239] The test object of Comparative Example 6 is a firming and anti-aging composition prepared by using a sterilized wet mixed powder fermented with an Aspergillus oryzae suspension;

[0240] The test object of Comparative Example 7 is a firming and anti-aging composition prepared by using Pyracantha fortuneana fruit powder and Pyracantha fortuneana fruit extract beyond the proportion range;

[0241] The test object of Comparative Example 8 is a firming and anti-aging composition prepared by mixing Pyracantha fortuneana fruit fermentation product and recombinant type III humanized collagen beyond the ratio range.

[0242] In summary, it can be concluded that the firming and anti-aging compositions prepared in Examples 1-5 have good inhibition rates on elastase, and are higher than those in Comparative Examples 1-8, indicating that the firming and anti-aging compositions prepared by the specified process and components have certain firming and anti-aging effects on the skin.

[0243] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A firming and anti-aging composition, characterized in that: Calculated by mass, it contains the following components: 1-5 servings of Pyracantha fruit fermentation 0.01-0.05 parts of roxburghii extract Malachite extract 0.5-3 parts Recombinant humanized type III collagen 2-6 copies Auxiliary agent 0.1-0.5 parts; Calculated by weight, the Pyracantha fortuneana fruit fermented product is prepared by the following steps: W1. 8-12 parts of Pyracantha fruit are washed with water and dried, ground into Pyracantha fruit powder, mixed with 4-8 parts of Pyracantha fruit extract, moistened with distilled water, maintaining a water content of 50-60% by volume, and sterilized at 110-120 ° C for 20-40min to obtain a sterilized wet mixed powder; W2. 200-300 mL of the Bacillus subtilis suspension was evenly sprinkled on 1500-2000 g of sterilized wet mixed powder, the initial pH was adjusted to 6-8, cultured at 34-38 ° C for 40-48 h, then sterilized, cooled, filtered to remove residue, centrifuged at 4000-4200 r / min, filtered through a membrane, and the filtrate obtained was the filtrate obtained as the fermented product of Pyracantha fruit; The cell concentration of the Bacillus subtilis suspension in step W2 is 1×10 8 -5×10 8 CFU / mL.

2. A firming and anti-aging composition according to claim 1, characterized in that: Calculated by mass, it contains the following components: 2-4 servings of fermented pyracantha fruit 0.02-0.04 parts of roxburghii extract Malachite extract 1-2 parts 3-5 copies of recombinant humanized type III collagen Auxiliary agent 0.2-0.4 parts.

3. A firming and anti-aging composition according to claim 1, characterized in that: The auxiliary agent consists of 1-5 parts of menthol and 4-8 parts of oligosphingosine.

4. A firming and anti-aging composition according to claim 1, characterized in that: The auxiliary agent consists of 2-3 parts of menthol and 5-7 parts of oligosphingosine.

5. The firming and anti-aging composition according to claim 1, characterized in that: The firming and anti-aging composition is applied to a leave-on skin external preparation.

6. A firming and anti-aging composition according to claim 5, characterized in that: The addition ratio of the firming and anti-aging composition in the leave-on skin external preparation is ≤10wt%.

7. A firming and anti-aging composition according to claim 5, characterized in that: The addition ratio of the firming and anti-aging composition in the leave-on skin external preparation is ≤5wt%.

8. The firming and anti-aging composition according to claim 5, characterized in that: The dosage form of the leave-on skin external preparation is emulsion, gel or cream.

Images