Anaerobic strain cultivation method and device

Abstract

The invention belongs to the technical field of anaerobic bacteria culture, and particularly relates to an anaerobic bacteria culture method and device, the device comprises a culture box, a plurality of mutually independent chambers are arranged in the culture box, the front end of the culture box is provided with door bodies I which are arranged in one-to-one correspondence with the plurality of chambers, the side surface of the culture box is provided with a connecting port, and the connecting port is connected with the door bodies I; a gate is hinged to the outer side of the connecting opening, and a deoxidizing bin is arranged on the same side of the outer portion of the cultivation box and the connecting opening; the deoxygenization bin is connected with the connecting port through a butt joint mechanism, and the top of the deoxygenization bin is connected with a deoxygenization mechanism; the docking mechanism comprises a fixed cylinder fixedly mounted on the deoxygenization bin, and the deoxygenization bin is arranged and the deoxygenization mechanism is mounted on the deoxygenization bin, so that deoxygenization operation can be carried out when nutrient substances need to be added into a culture dish or other detection operations need to be carried out in the bacterial culture process; and the situation that anaerobic bacteria in the culture dish are exposed in an aerobic environment and the culture work is influenced due to each operation is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of anaerobic bacteria cultivation, and in particular relates to a method and a device for cultivating anaerobic bacteria. Background Art

[0002] Anaerobic bacteria, also known as anaerobic bacteria, are a type of bacteria that grow better under anaerobic conditions than in aerobic environments, and cannot grow on the surface of solid culture media in air (18% oxygen) and / or 10% carbon dioxide concentrations. During the culture process, bacteria need to be provided with suitable nutrients, temperature, pH value, etc. Therefore, when culturing bacteria, nutrients need to be added to the culture dish according to the culture conditions to facilitate bacterial growth.

[0003] For example, the anaerobic bacteria cultivation device for manure treatment provided by the Chinese patent application publication number CN114561267A includes a base, on which a crushing and stirring device and a fermentation device are arranged, and the stirring device is connected to the fermentation device; the fermentation device includes a box body, and a sliding door is arranged on the front of the box body, and the sliding door is used to seal the box body, and the box body has a mixing area and a fermentation area inside, and an anaerobic bacteria mixing box is arranged in the mixing area, and the other end passes through the box body and is connected to the inside of the anaerobic bacteria mixing box, and the sealing door is used to seal the inside of the anaerobic bacteria mixing box, etc.; the device can cultivate anaerobic bacteria and perform comparative operations of cultivation, and has a good use effect.

[0004] As described in the prior art of the above patent, in the anaerobic bacteria culture, the equipment does not have the function of eliminating oxygen. Therefore, in the process of culturing anaerobic bacteria, each time nutrients are added to the culture dish or the pH value is tested, the incubator needs to be opened, which easily exposes the anaerobic bacteria to the aerobic environment, thereby being detrimental to the growth of the anaerobic bacteria. Summary of the invention

[0005] The purpose of the present invention is to provide a method and device for cultivating anaerobic bacteria, which can deoxygenate the culture environment of anaerobic bacteria and avoid the adverse effects of anaerobic bacteria growth caused by exposure of anaerobic bacteria to an aerobic environment during the culture process.

[0006] The technical solution adopted by the present invention is as follows: A method and device for cultivating anaerobic bacteria, comprising a cultivation box, wherein a plurality of chambers independent of each other are arranged in the cultivation box, a door body corresponding to the plurality of chambers is arranged at the front end of the cultivation box, a connection port is provided on the side of the cultivation box, a gate is hingedly connected to the outer side of the connection port, the gate can be opened toward the inner side of the chamber, and when the gate is in the maximum open state, the gate is arranged flush with the bottom surface of the chamber, a deoxygenation bin is arranged on the same side of the connection port as the outer side of the cultivation box; the deoxygenation bin is connected to the connection port by a docking mechanism, and the top of the deoxygenation bin is connected with a deoxygenation mechanism; the docking mechanism comprises a fixed cylinder fixedly mounted on the deoxygenation bin, a movable cylinder is sleeved on the fixed cylinder, and a telescopic rod is connected to the outer side of the movable cylinder, wherein the telescopic rod has a first stroke and a second stroke in the extended state, the movable cylinder extends into the inner side of the connection port when the telescopic rod is in the first stroke state, and the telescopic rod drives the movable cylinder to push the gate open in the second stroke state, and the gate is in a horizontal state flush with the bottom surface of the chamber.

[0007] In a preferred embodiment, each chamber is provided with a connecting port in a one-to-one correspondence, and the connecting port and the chamber are in a communicating structure.

[0008] In a preferred embodiment, the front end of the deoxygenation bin is hinged with door body 2, the outer side of the deoxygenation bin is connected to a lifting mechanism, the structure of door body 1 and door body 2 includes an external frame and an inner plate, and the structure of the inner plate is a transparent structure.

[0009] In a preferred embodiment, an isolation operation glove 1 is connected to the inner plate of the door body 1, and an isolation operation glove 2 is connected to the inner plate of the door body 2. The materials of the isolation operation glove 1 and the isolation operation glove 2 are both elastic rubber materials.

[0010] In a preferred embodiment, an auxiliary heating plate is installed on the back of the incubator, and the auxiliary heating plate is arranged in a one-to-one correspondence with the chamber, and an oxygen content sensor is arranged on the other side of the incubator.

[0011] In a preferred embodiment, the deoxygenation mechanism includes a placement port connected to the deoxygenation bin, a holding basket is provided inside the placement port, and the holding basket is used to place the deoxygenator, and a cover plate is installed at the top of the placement port.

[0012] In a preferred embodiment, the containing basket and the placement port are connected by a snap-fit ​​connection, the containing basket is provided with holes, and the diameter of the holes is smaller than the diameter of the deoxygenator particles contained therein, the cover plate and the placement port are connected by a snap-fit ​​connection, the top end of the cover plate is connected to a connecting pipe, the other end of the connecting pipe is connected to a micro vacuum pump, and the other end of the micro vacuum pump is connected to the deoxygenation bin.

[0013] In a preferred embodiment, the lifting mechanism includes a vertically placed track, an adjustment block is slidably arranged in the track, a screw rod is rotatably connected in the track, and the screw rod and the adjustment block are threadedly engaged, a servo motor is connected to the top end of the screw rod, and the outer side surface of the deoxygenation bin is connected to the adjustment block.

[0014] In a preferred embodiment, a control structure is further provided on the outside of the incubator, and the control structure is electrically connected to the docking mechanism, the deoxygenation mechanism, the lifting mechanism, the auxiliary heating plate and the oxygen content sensor through wires.

[0015] An anaerobic bacteria cultivation method, applied to the above-mentioned anaerobic bacteria cultivation device, comprises the following steps: S1. Place the culture dish with anaerobic bacteria into the chamber of the incubator and close door 1; S2. During the anaerobic bacteria cultivation process, when nutrients need to be added to the culture dish according to the cultivation situation, the nutrients are first placed in the deoxygenation chamber, and the second door is closed, and the docking mechanism is extended to the inner side of the connection port. At this time, the telescopic rod is in the first stroke state; S3, start the deoxygenation mechanism to adsorb the oxygen in the deoxygenation chamber to achieve the purpose of removing oxygen, and let it stand for a period of time so that the oxygen can be completely adsorbed; S4. Then, the telescopic rod is controlled to continue to extend and reach the second stroke, and the movable cylinder is driven to continue to extend through the telescopic rod, and the gate is pushed open, and then the nutrients placed in the deoxygenation chamber in the early stage are moved to the incubator, and then the nutrients are taken through the isolation operation gloves and added to the culture dish.

[0016] The technical effects achieved by the present invention are: 1. The present invention provides a deoxygenation chamber and installs a deoxygenation mechanism on the deoxygenation chamber. When it is necessary to add nutrients to the culture dish or perform other detection operations during the bacterial culture process, the components can be placed in the deoxygenation chamber first for deoxygenation operations, thereby avoiding that each operation causes the anaerobic bacteria in the culture dish to be exposed to an aerobic environment again and again, thereby affecting the cultivation work. The anaerobic bacteria can always be in an anaerobic environment during the cultivation process, thereby increasing the growth rate of the anaerobic bacteria. 2. The incubator of the present invention is provided with several independent chambers, and different anaerobic bacteria can be placed in different chambers for cultivation, so as to avoid the mutual influence of different cultivation conditions required by different anaerobic bacteria, facilitate the cultivation of multiple strains, and improve the efficiency of cultivation; 3. The present invention can drive the deoxygenation bin and the docking mechanism to adjust the height through the lifting mechanism, so that the deoxygenation bin can operate corresponding to chambers at different levels, that is, one deoxygenation bin can be used for multiple chambers, making the use of the equipment more flexible and diverse. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention; Figure 2 is a schematic diagram of the structure between the incubator, the chamber and the door body 1 according to an embodiment of the present invention; Figure 3 is a cross-sectional enlarged structural schematic diagram of an incubator and a connection port of an embodiment of the present invention; Figure 4 is a half-section enlarged structural schematic diagram of the auxiliary heating plate and the incubator in an embodiment of the present invention; Figure 5 It is an enlarged structural schematic diagram of the deoxygenation bin, the docking mechanism and the lifting mechanism of an embodiment of the present invention; Figure 6 is a structural schematic diagram of a telescopic rod in a docking mechanism of an embodiment of the present invention in a retracted state; Figure 7 is an enlarged structural schematic diagram of a telescopic rod in a docking mechanism of an embodiment of the present invention in a first stroke state; Figure 8 is an enlarged structural schematic diagram of a telescopic rod in a docking mechanism of an embodiment of the present invention being in a second stroke state; Fig. 9 It is a schematic diagram of the exploded and enlarged structure between the deoxygenation bin and the deoxygenation mechanism of an embodiment of the present invention; Fig.10 It is a half-sectioned enlarged structural schematic diagram of the lifting mechanism of an embodiment of the present invention;.

[0018] In the accompanying drawings, the components represented by the reference numerals are listed as follows: 1. Incubator; 2. Chamber; 3. Door body one; 31. Isolation operation gloves one; 4. Connecting port; 41. Sealing ring; 5. Gate; 51. Strong magnet; 6. Deoxidation chamber; 7. Docking mechanism; 71. Fixed cylinder; 72. Movable cylinder; 73. Telescopic rod; 8. Deoxidation mechanism; 81. Connecting pipe; 82. Micro vacuum pump; 83. Placement port; 84. Container basket; 85. Cover plate; 9. Door body 2; 91. Isolation operation gloves 2; 10. Lifting mechanism; 101. Track; 102. Adjusting block; 103. Screw rod; 104. Servo motor; 11. Control structure; 12. Auxiliary heating plate; 13. Oxygen content sensor. DETAILED DESCRIPTION

[0019] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.

[0020] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0021] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. The term "in a preferred embodiment" that appears in different places in this specification does not refer to the same embodiment, nor is it a separate or selective embodiment that is mutually exclusive with other embodiments.

[0022] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0023] See also Figure 1 and Figure 6-Figure 8As shown, the present invention provides an anaerobic bacteria cultivation method and device, comprising: a cultivation box 1, wherein a plurality of mutually independent chambers 2 are arranged in the cultivation box 1, a door body 3 arranged one-to-one corresponding to the plurality of chambers 2 is arranged at the front end of the cultivation box 1, a connection port 4 is opened on the side of the cultivation box 1, a gate 5 is hingedly connected to the outer side of the connection port 4, the gate 5 can be opened toward the inner side of the chamber 2, and when the gate 5 is in the maximum open state, the gate 5 is arranged flush with the bottom surface of the chamber 2, and a deoxygenation bin 6 is arranged on the same side of the outside of the cultivation box 1 and the connection port 4; a passage is provided between the deoxygenation bin 6 and the connection port 4. The deoxygenation bin 6 is connected through a docking mechanism 7, and the top of the deoxygenation bin 6 is connected with a deoxygenation mechanism 8; the docking mechanism 7 includes a fixed cylinder 71 fixedly installed on the deoxygenation bin 6, a movable cylinder 72 is sleeved on the fixed cylinder 71, and a telescopic rod 73 is connected to the outer side of the movable cylinder 72, wherein the telescopic rod 73 has a first stroke and a second stroke in the extended state, and the telescopic rod 73 is in the first stroke state, and the movable cylinder 72 extends into the inner side of the connecting port 4, and the telescopic rod 73 is in the second stroke state, driving the movable cylinder 72 to push open the gate 5, and making the gate 5 in a horizontal state flush with the bottom surface of the chamber 2; The culture dish is placed in the chamber 2 of the incubator 1, and the door 3 is closed for the incubation operation. During the anaerobic bacteria incubation process, when it is necessary to add nutrients to the culture dish or perform other detection operations, the components can be first placed in the deoxygenation bin 6 for deoxygenation operation to avoid the anaerobic bacteria in the culture dish being exposed to the aerobic environment again and again during each operation, which affects the incubation work. The anaerobic bacteria can always be in an anaerobic or low-oxygen environment during the incubation process, thereby increasing the growth rate of the anaerobic bacteria. In addition, when cultivating anaerobic bacteria, before the culture dish is placed in the chamber 2 of the cultivation box 1, in order to make the chamber 2 in an anaerobic or hypoxic state to facilitate the growth and reproduction of anaerobic bacteria, the chamber 2 of the cultivation box 1 can be deoxygenated first. The specific operation steps are as follows: first, the door body 1 3 of the cultivation box 1 is closed to make the chamber 2 in a closed state, and then the door body 2 9 is closed, and then the docking mechanism 7 is extended to make the movable cylinder 72 of the docking mechanism 7 extend into the interior of the connecting port 4 and push the gate 5 open. At this time, the travel of the telescopic rod 73 is directly in the second formation, so that the chamber 2 is connected with the deoxygenation bin 6, so that the deoxygenation mechanism 8 on the deoxygenation bin 6 is used to deoxygenate the chamber 2; After the deoxygenation of the chamber 2 is completed, the retraction of the telescopic rod 73 drives the movable cylinder 72 to be drawn out from the connecting port 4, and when the movable cylinder 72 is drawn out, the pressure on the gate 5 is released at the same time. Since the gate 5 is hinged to the inner wall of the chamber 2 by a hinge with a torsion spring, when the gate 5 is opened, the torsion spring on the hinge is deformed to generate elastic force. When the movable cylinder 72 is drawn out, the gate 5 is driven to rotate upward along the hinge by the elastic force of the torsion spring on the hinge, and is sealed at the connecting port 4 for sealing. After the deoxygenation is completed, the culture dish placed in the chamber 2 of the incubator 1 is in an anaerobic or hypoxic environment to the greatest extent, which is convenient for the growth and reproduction of anaerobic bacteria in the culture dish. When it is necessary to add nutrients to the culture dish or perform other detection operations during the anaerobic bacterial culture process, first place the nutrients to be added or other instruments used for other detection in the deoxygenation chamber 6, close the door body 9, and extend the telescopic rod 73 in the docking mechanism 7 to drive the movable cylinder 72 to extend into the inner side of the connecting port 4, and the front end of the movable cylinder 72 does not contact the gate 5. At this time, the telescopic rod 73 is in the first stroke state, and then the air inside the deoxygenation chamber 6 is deoxygenated through the deoxygenation mechanism 8. The movable cylinder 72 is in the state of extending into the connecting port 4 in order to connect the deoxygenation chamber 6 and the incubator 1 as a whole to avoid communication with the outside world, so that the deoxygenation work is more effective. After the deoxygenation is completed, the telescopic rod 73 continues to extend. The movable cylinder 72 extends, driving the movable cylinder 72 to continue to extend into the chamber 2 in the connecting port 4 until the gate 5 is pushed open by the movable cylinder 72. At this time, the telescopic rod 73 is in the second stroke, so that the connecting port 4 is opened, and the nutrients or other instruments used for other tests in the deoxygenation bin 6 that have been deoxygenated can be transferred to the chamber 2, which is convenient for operating the culture dish in the chamber 2. The width and height of the connecting port 4 are the same as the width and height of the outer wall of the movable cylinder 72, and the connecting port 4 is provided with a sealing ring 41, which can fill the sealing of the connection between the connecting port 4 and the movable cylinder 72 to avoid air leakage. The gate 5 and the inner wall of the chamber 2 are respectively embedded with strong magnets 51, which can make the gate 5 close more tightly when the gate 5 is closed to avoid loose closure.

[0024] In a preferred embodiment, please refer to Figure 3 As shown, each chamber 2 is provided with a connecting port 4 in a one-to-one correspondence, and the connecting port 4 and the chamber 2 are in a communicating structure; As shown in the above structure, each chamber 2 is provided with a corresponding connection port 4, which can facilitate each chamber 2 to be connected to the deoxygenation bin 6, so that each chamber 2 is more independent, and a gate 5 is provided at each connection port 4 to control the opening and closing of the connection port 4; The incubator 1 is provided with a plurality of independent chambers 2, and different anaerobic bacteria can be placed in different chambers 2 for cultivation, so as to avoid the different cultivation conditions required by different anaerobic bacteria from influencing each other, facilitate the cultivation of multiple strains, and improve the cultivation efficiency.

[0025] Second, see Figure 2 and Fig. 9 As shown, the front end of the deoxygenation bin 6 is hinged with a door body 2 9, the outer side of the deoxygenation bin 6 is connected to the lifting mechanism 10, the structure of the door body 1 3 and the door body 2 9 includes an external frame and an inner plate, and the structure of the inner plate is a transparent structure, the inner plate of the door body 1 3 is connected with an isolation operation glove 1 31, and the inner plate of the door body 2 9 is connected with an isolation operation glove 2 91, and the materials of the isolation operation glove 1 31 and the isolation operation glove 2 91 are both made of elastic rubber material; The door body 1 3 and the door body 2 9 serve as the closed structures of the incubator 1 and the deoxygenation chamber 6 respectively, and both can be sealed when they need to be closed, and opened when they need to be opened. Transparent inner panels are arranged on the door body 1 3 and the door body 2 9, so that the internal situation can be observed from the outside, which is convenient for operation. By using the isolation operation gloves 1 31 and the isolation operation gloves 2 91, when operating the interior, the hand can be inserted into the isolation operation gloves 1 31 or the isolation operation gloves 2 91 to operate through the gloves. This can meet the needs of operation and avoid the need to open the door body during operation to allow oxygen to enter and affect the growth of bacteria. The transparent inner panel can be made of glass or transparent acrylic board.

[0026] In a preferred embodiment, please refer to Figure 4 As shown, an auxiliary heating plate 12 is installed on the back of the incubator 1, and the auxiliary heating plate 12 is arranged one-to-one with the chamber 2, and an oxygen content sensor 13 is arranged on the other side of the incubator 1; In this embodiment, during the cultivation of anaerobic bacteria, the oxygen content in the chamber 2 can be monitored in real time by the oxygen content sensor 13, and the measured signal value is transmitted to the control structure 11, which is displayed and analyzed by the control structure 11, so that the operator can monitor the oxygen content in the chamber 2 in real time, and the oxygen content sensor 13 can be a sensor of the brand model DDS-S+4OX. When the temperature environment is lower than the set temperature, the control structure 11 can control the auxiliary heating plate 12 to start working, and perform auxiliary heating on the chamber 2, so that the anaerobic bacteria can grow at a suitable temperature.

[0027] In a preferred embodiment, please refer to Figure 5 and Fig. 9As shown, the deoxygenation mechanism 8 includes a placement port 83 connected to the deoxygenation bin 6, a holding basket 84 is provided inside the placement port 83, and the holding basket 84 is used to place the deoxygenant, a cover plate 85 is installed at the top of the placement port 83, the holding basket 84 and the placement port 83 are snap-fitted, holes are provided on the holding basket 84, and the diameter of the holes is smaller than the diameter of the deoxygenant particles contained, the cover plate 85 and the placement port 83 are snap-fitted, a connecting pipe 81 is connected to the top of the cover plate 85, the other end of the connecting pipe 81 is connected to the micro air pump 82, and the other end of the micro air pump 82 is connected to the deoxygenation bin 6; As in the above structure, the oxygen in the deoxygenation bin 6 is deoxygenated by the deoxygenation mechanism 8. During the operation, the holding basket 84 is clamped in the placement port 83, and a proper amount of deoxygenator is poured into the holding basket 84, and then the cover plate 85 is covered to isolate it from the outside, so that the deoxygenator in the holding basket 84 can absorb the oxygen in the deoxygenation bin 6 to the maximum extent, thereby achieving the purpose of deoxygenating the deoxygenation bin 6. In order to make the deoxygenation more efficient, the micro air pump 8 can be connected to the connecting pipe 81. 2 and the cover plate 85, and start the micro vacuum pump 82 to extract the air in the deoxygenation chamber 6 and pass through the deoxygenator in the holding basket 84, and then pump it back into the deoxygenation chamber 6, so that the air in the deoxygenation chamber 6 flows, thereby making the deoxygenation efficiency higher, and the buckling connection between the cover plate 85 and the placement port 83 can facilitate the disassembly and installation between the two, and is convenient for replacing the internal deoxygenator, and the deoxygenator is used with a particle diameter larger than the diameter of the hole on the holding basket 84 to avoid leakage from the hole.

[0028] In a preferred embodiment, please refer to Fig.10 As shown, the lifting mechanism 10 includes a vertically placed track 101, an adjusting block 102 is slidably arranged in the track 101, a screw rod 103 is rotatably connected in the track 101, and the screw rod 103 and the adjusting block 102 are threadedly engaged, a servo motor 104 is connected to the top of the screw rod 103, and the outer side of the deoxygenation bin 6 is connected to the adjusting block 102; The height adjustment of the deoxygenation bin 6 is driven by the lifting mechanism 10. When in use, the forward and reverse rotation of the servo motor 104 is controlled by the control structure 11, and the servo motor 104 drives the screw rod 103 to rotate. The screw rod 103 and the adjusting block 102 are threadedly engaged, so that the adjusting block 102 can be driven to adjust the height up and down in the track 101, thereby driving the deoxygenation bin 6 and the auxiliary equipment to adjust the height up and down, so as to meet the needs of the deoxygenation bin 6 for use with different chambers 2. The lifting mechanism 10 can drive the deoxygenation bin 6 and the docking mechanism 7 to adjust the height, so that the deoxygenation bin 6 can operate corresponding to chambers 2 at different levels, that is, one deoxygenation bin 6 can be used for multiple chambers 2, making the use of the equipment more flexible and diverse.

[0029] A control structure 11 is also provided on the outside of the incubator 1, and the control structure 11 is electrically connected to the docking mechanism 7, the deoxygenation mechanism 8, the lifting mechanism 10, the auxiliary heating plate 12, and the oxygen content sensor 13 through wires; The control structure 11 is used to control the operation of the device, so that the normal use of the device can be met. The control structure 11 is used to control the extension and retraction of the telescopic rod 73 in the docking mechanism 7, the opening and closing of the micro vacuum pump 82 of the deoxygenation mechanism 8, and the forward and reverse rotation of the servo motor 104 of the lifting mechanism 10, so that the device can be used normally.

[0030] An anaerobic bacteria cultivation method, applied to the above-mentioned anaerobic bacteria cultivation device, comprises the following steps: S1, placing the culture dish with anaerobic bacteria into the chamber 2 of the incubator 1, and closing the door 3; S2. During the anaerobic bacteria cultivation process, when nutrients need to be added to the culture dish according to the cultivation situation, the nutrients are first placed in the deoxygenation chamber 6, and the door 9 is closed, and the docking mechanism 7 is extended to the inner side of the connecting port 4. At this time, the telescopic rod 73 is in the first stroke state; S3, start the deoxygenation mechanism 8 to adsorb the oxygen in the deoxygenation bin 6 to achieve the purpose of removing oxygen, and let it stand for a period of time so that the oxygen can be completely adsorbed; S4. Then, the telescopic rod 73 is controlled to continue to extend and reach the second stroke, and the movable cylinder 72 is driven to continue to extend through the telescopic rod 73, and the gate 5 is pushed open, and then the nutrients placed in the deoxygenation chamber 6 in the early stage are moved to the incubator 1, and then the nutrients are taken through the isolation operation gloves 31 and added to the culture dish.

[0031] The above is only a preferred embodiment of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principles of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the art unless otherwise specified and limited.

Claims

1. An anaerobic bacteria cultivation device, characterized in that: include: A cultivation box (1), wherein a plurality of mutually independent chambers (2) are arranged in the cultivation box (1), a door body (3) is arranged at the front end of the cultivation box (1) and is arranged in a one-to-one correspondence with the plurality of chambers (2), a connection port (4) is provided on the side of the cultivation box (1), a gate (5) is hingedly connected to the outer side of the connection port (4), the gate (5) can be opened toward the inner side of the chamber (2), and when the gate (5) is in the maximum open state, the gate (5) is arranged flush with the bottom surface of the chamber (2), and a deoxygenation chamber (6) is arranged on the outside of the cultivation box (1) and on the same side as the connection port (4); The deoxygenation bin (6) and the connection port (4) are connected via a docking mechanism (7), and the top of the deoxygenation bin (6) is connected to a deoxygenation mechanism (8); The docking mechanism (7) comprises a fixed cylinder (71) fixedly mounted on the deoxygenation bin (6), a movable cylinder (72) being sleeved on the fixed cylinder (71), and a telescopic rod (73) being connected to the outer side of the movable cylinder (72), wherein the telescopic rod (73) has a first stroke and a second stroke in an extended state, and when the telescopic rod (73) is in the first stroke state, the movable cylinder (72) extends into the inner side of the connection port (4), and when the telescopic rod (73) is in the second stroke state, it drives the movable cylinder (72) to push open the gate (5), and places the gate (5) in a horizontal state flush with the bottom surface of the chamber (2).

2. The anaerobic bacteria cultivation device according to claim 1, characterized in that: Each chamber (2) is provided with a connecting port (4) in a one-to-one correspondence, and the connecting port (4) and the chamber (2) are in a communicating structure.

3. The anaerobic bacteria cultivation device according to claim 1, characterized in that: The front end of the deoxygenation bin (6) is hingedly connected to a second door body (9), the outer side of the deoxygenation bin (6) is connected to a lifting mechanism (10), the structure of the first door body (3) and the second door body (9) comprises an external frame and an inner plate, and the structure of the inner plate is a transparent structure.

4. The anaerobic bacteria cultivation device according to claim 3, characterized in that: An isolation operation glove 1 (31) is connected to the inner plate of the door body 1 (3), and an isolation operation glove 2 (91) is connected to the inner plate of the door body 2 (9). The isolation operation glove 1 (31) and the isolation operation glove 2 (91) are both made of elastic rubber.

5. The anaerobic bacteria cultivation device according to claim 1, characterized in that: An auxiliary heating plate (12) is installed on the back of the incubator (1), and the auxiliary heating plate (12) and the chamber (2) are arranged in a one-to-one correspondence. An oxygen content sensor (13) is arranged on the other side of the incubator (1).

6. The anaerobic bacteria cultivation device according to claim 1, characterized in that: The deoxygenation mechanism (8) comprises a placement opening (83) connected to the deoxygenation bin (6); a holding basket (84) is provided inside the placement opening (83); and the holding basket (84) is used to place the deoxygenating agent; a cover plate (85) is installed at the top end of the placement opening (83).

7. The anaerobic bacteria cultivation device according to claim 6, characterized in that: The containing basket (84) and the placement opening (83) are connected by snap-fitting, the containing basket (84) is provided with holes, and the diameter of the holes is smaller than the diameter of the deoxidizer particles contained therein, the cover plate (85) and the placement opening (83) are connected by snap-fitting, the top end of the cover plate (85) is connected to a connecting pipe (81), the other end of the connecting pipe (81) is connected to a micro vacuum pump (82), and the other end of the micro vacuum pump (82) is connected to the deoxidation bin (6).

8. The anaerobic bacteria cultivation device according to claim 3, characterized in that: The lifting mechanism (10) comprises a vertically placed track (101), an adjusting block (102) is slidably arranged in the track (101), a screw rod (103) is rotatably connected in the track (101), and the screw rod (103) and the adjusting block (102) are threadedly engaged, a servo motor (104) is connected to the top end of the screw rod (103), and the outer side surface of the deoxygenation bin (6) is connected to the adjusting block (102).

9. The anaerobic bacteria cultivation device according to claim 5, characterized in that: A control structure (11) is also provided on the outside of the incubator (1), and the control structure (11) is electrically connected to the docking mechanism (7), the deoxygenation mechanism (8), the lifting mechanism (10), the auxiliary heating plate (12), and the oxygen content sensor (13) through wired connections.

10. An anaerobic bacteria cultivation method, applied to the anaerobic bacteria cultivation device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Place a culture dish containing anaerobic bacteria into the chamber (2) of the incubator (1), and close the door (3); S2. During the anaerobic bacteria cultivation process, when nutrients need to be added to the culture dish according to the cultivation conditions, the nutrients are first placed in the deoxygenation chamber (6), the second door (9) is closed, and the connecting mechanism (7) is extended to the inner side of the connecting port (4). At this time, the telescopic rod (73) is in the first stroke state; S3, starting the deoxygenation mechanism (8) to adsorb the oxygen in the deoxygenation chamber (6) to achieve the purpose of removing oxygen, and leaving it to stand for a period of time so that the oxygen can be completely adsorbed; S4. Then, the telescopic rod (73) is controlled to continue to extend and reach the second stroke, and the movable cylinder (72) is driven to continue to extend through the telescopic rod (73), and the gate (5) is pushed open, and then the nutrients placed in the deoxygenation chamber (6) are moved to the incubator (1), and then the nutrients are taken through the isolation operation glove (31) and added to the culture dish.

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