Extraction device for biological gene sample

Abstract

The invention belongs to the technical field of biological gene sample extraction, and relates to a biological gene sample extraction device, which comprises a storage tank body, a tank cover, an opening and closing cover plate, an isolation cylinder body, a rotating support and a placing disc, the top of the storage tank body is connected with the tank cover, and the top of the tank cover is rotatably connected with the opening and closing cover plate; the bottom of the tank cover is connected with an isolation cylinder body, four rotating brackets are uniformly and rotatably connected outside the isolation cylinder body at intervals, the lower part of each rotating bracket is connected with a placing disc, a plurality of clamping grooves for accommodating test tubes are uniformly formed in the placing disc at intervals, and four through holes are uniformly formed in the isolation cylinder body at intervals. In the process of extracting a biological gene sample, a test tube is firstly extracted into the feeding cylinder, the feeding cylinder moves upwards to convey the test tube upwards, after the feeding cylinder moves upwards, cold air can be isolated through the bearing disc, in the process of opening the opening and closing cover plate to extract the test tube, the cold air cannot escape, and therefore loss of liquid nitrogen cold air is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of biological gene sample extraction and relates to a device for extracting biological gene samples. Background Art

[0002] With the progress of biomedical research, the importance of biobanks has become increasingly prominent. They not only provide researchers with a large number of sample resources, but also promote international cooperation and resource sharing, and accelerate the transformation of medical research results into clinical applications. In the research of biological gene sample banks, the preservation and extraction of biological gene samples is a crucial task.

[0003] At present, when biological gene samples are preserved, in order to ensure the activity and stability of the samples, the samples need to be stored in an extremely low temperature environment, such as liquid nitrogen or an ultra-low temperature refrigerator. However, after the existing biological gene samples are stored, the storage mechanism needs to be opened during the extraction process, which will cause a large amount of cold air to leak out. If a refrigerator is used for refrigeration, the burden on the refrigeration system is increased, forcing the refrigerator to perform secondary refrigeration. If liquid nitrogen is used for storage, the liquid nitrogen cold air is lost and liquid nitrogen needs to be replenished, resulting in unnecessary energy consumption and economic losses, which seriously affects the practicality and economy of the storage system. Summary of the invention

[0004] In view of this, the present invention provides a device for extracting biological gene samples.

[0005] The technical solution is: a device for extracting biological gene samples, including a storage tank body, a tank cover, an opening and closing cover plate, an isolation cylinder, a rotating bracket, a placement plate, a driving rotating mechanism, an adding mechanism and a sampling mechanism, the top of the storage tank body is connected to the tank cover, the top of the tank cover is rotatably connected to the opening and closing cover plate, the bottom of the tank cover is connected to the isolation cylinder, four rotating brackets are rotatably connected to the outside of the isolation cylinder at even intervals, each rotating bracket is connected to a placement plate at the bottom, a plurality of slots for accommodating test tubes are evenly spaced on the placement plate, four through openings are evenly spaced on the isolation cylinder, a driving rotating mechanism for driving the rotating bracket to rotate is provided between the rotating bracket and the tank cover, and an adding mechanism for adding test tubes and a sampling mechanism for pushing and sampling test tubes are provided in the isolation cylinder.

[0006] As an improvement of the above solution, a threaded rod and a locking nut are also included. The tank cover is connected to the threaded rod, and the locking nut can be screwed into the threaded rod through a thread. The threaded rod and the locking nut are used to lock the opening and closing cover plate.

[0007] As an improvement on the above scheme, the driving rotation mechanism includes an inner gear ring, a rotating shaft, a driving motor and a driving gear. The rotating bracket is connected to the inner gear ring, the isolation cylinder is rotatably connected to the rotating shaft, four driving gears are evenly spaced and connected to the rotating shaft, the driving gears are meshed with the inner gear ring, and a driving motor is installed on the top of the tank cover, and the output shaft of the driving motor is connected to the rotating shaft.

[0008] As an improvement of the above-mentioned scheme, the adding mechanism includes a partition plate, a guide cylinder, a feeding cylinder, a supporting tray, a servo motor and a driving screw. The guide cylinder is connected to the isolation cylinder body, the partition plate is connected to the upper part of the isolation cylinder body, the partition plate is connected to the guide cylinder, the feeding cylinder is slidably connected to the guide cylinder, the lower part of the feeding cylinder is connected to the supporting tray, the feeding cylinder and the supporting tray can support and transport the test tubes, a servo motor is installed on the top of the partition plate, the output shaft of the servo motor is connected to the driving screw, and the driving screw is threadedly matched with the feeding cylinder.

[0009] As an improvement on the above scheme, the sampling mechanism includes a sliding frame, a sliding rod, an elastic member 1, a sliding plate, a pushing rod, an extension shaft, a pushing arc plate and an elastic member 2. The partition plate and the tank cover are both slidably connected with sliding rods, the bottom of the sliding rod is connected to the sliding frame, an elastic member 1 is connected between the sliding rod and the partition plate, a plurality of sliding plates are evenly spaced and connected to the sliding frame, four pushing rods are evenly spaced and slidably connected to the guide cylinder, an extension shaft is connected to the lower part of the pushing rod, oblique grooves are provided on both sides of the sliding plate, the extension shaft is located in the oblique grooves, two pushing arc plates are rotatably connected to the push rod, and an elastic member 2 is connected between the two pushing arc plates.

[0010] As an improvement of the above scheme, a blocking mechanism is also included, which includes a blocking plate, a blocking plate and an elastic member three. A discharge port is opened in the middle of the supporting tray, and a blocking plate is connected to the lower part of the feeding barrel. The blocking plate is located below the supporting tray, and a blocking plate is slidably connected to the blocking plate. An elastic member three is connected between the blocking plate and the blocking plate.

[0011] As an improvement of the above solution, it also includes a limiting ring, and the upper part of the feeding cylinder is connected to a limiting ring for limiting the test tube.

[0012] As an improvement of the above scheme, a limiting mechanism is also included, which includes a push rod, four elastic parts and a blocking ring. A push rod is slidably connected between the tank cover and the partition plate, and four elastic parts are connected between the push rod and the tank cover. Four blocking rings are evenly spaced and connected to the push rod, and the blocking ring is located outside the isolation cylinder.

[0013] 1. In the process of extracting biological gene samples, the present invention first extracts the test tube into the feeding barrel, and then transports the test tube upward by moving the feeding barrel upward. After the feeding barrel moves upward, the cold air can be isolated by the supporting tray. In the process of opening the opening and closing cover to extract the test tube, the cold air will not escape, thereby avoiding the loss of liquid nitrogen cold air.

[0014] 2. After the test tube is placed, the present invention can drive the blocking ring to block the inner circle of the test tube through the push rod, limit the test tube, avoid the test tube from moving randomly, and improve the stability of the sample test tube during storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0016] Figure 2 It is a cross-sectional view of the present invention.

[0017] Figure 3 It is a structural schematic diagram of the driving rotation mechanism of the present invention.

[0018] Figure 4 This is a schematic diagram of the first structure of the adding mechanism and the sampling mechanism of the present invention.

[0019] Figure 5 This is a second structural schematic diagram of the adding mechanism and the sampling mechanism of the present invention.

[0020] Figure 6 It is a structural schematic diagram of the sampling mechanism of the present invention.

[0021] Figure 7 It is a top view of the sampling mechanism of the present invention.

[0022] Figure 8 It is a schematic diagram of the first structure of the blocking mechanism of the present invention.

[0023] Fig. 9 It is a second structural schematic diagram of the blocking mechanism of the present invention.

[0024] Fig.10 It is a structural schematic diagram of the limiting mechanism of the present invention.

[0025] Fig.11 It is a schematic diagram of the structure of the blocking ring, the placement plate and the rotating bracket of the present invention.

[0026] Names of the numbers in the figure: 1. storage tank body, 2. tank cover, 3. opening and closing cover plate, 41. threaded rod, 42. locking nut, 5. isolation cylinder, 6. rotating bracket, 7. placement plate, 8. through port, 91. inner gear ring, 92. rotating shaft, 93. driving motor, 94. driving gear, 101. partition plate, 102. guide cylinder, 103. feeding cylinder, 104. supporting tray, 105. servo motor, 106. driving screw, 111. sliding frame, 112. sliding rod, 113. elastic member one, 114. sliding plate, 115. pushing rod, 116. extension shaft, 117. pushing arc plate, 118. elastic member two, 121. blocking plate, 122. blocking plate, 123. elastic member three, 13. limiting ring, 141. pushing rod, 142. elastic member four, 143. blocking ring, 100. test tube. DETAILED DESCRIPTION

[0027] The above scheme is further described below in conjunction with specific examples. It should be understood that these examples are used to illustrate the present application and are not limited to the scope of the present application. The implementation conditions adopted in the examples can be further adjusted according to the conditions of the specific manufacturer, and the implementation conditions not specified are usually the conditions in conventional experiments.

[0028] A device for extracting biological gene samples, such as Figure 1-Figure 7 As shown, it includes a storage tank body 1, a tank cover 2, an opening and closing cover plate 3, a threaded rod 41, a locking nut 42, an isolation cylinder 5, a rotating bracket 6, a placement plate 7, a driving rotation mechanism, an adding mechanism and a sampling mechanism. The top of the storage tank body 1 is connected to the tank cover 2 by bolts, and the opening and closing cover plate 3 is rotatably connected to the right side of the top of the tank cover 2. The right side of the tank cover 2 is connected to the threaded rod 41, and the locking nut 42 can be screwed into the threaded rod 41 through a thread. The opening and closing cover plate 3 is provided with an opening, and the opening can be sleeved on the outside of the threaded rod 41. At this time, the locking nut 42 is rotated to the threaded rod 41 to open and close the cover. The plate 3 is locked, and an isolation cylinder 5 is connected to the bottom of the tank cover 2. Four rotating brackets 6 are rotatably connected to the outside of the isolation cylinder 5 at uniform intervals. A placement plate 7 is connected to the lower part of each rotating bracket 6. A plurality of slots for accommodating test tubes 100 are evenly spaced on the placement plate 7. Four through openings 8 are evenly spaced on the right side of the isolation cylinder 5. A driving rotating mechanism for driving the rotating bracket 6 to rotate is provided between the rotating bracket 6 and the tank cover 2. An adding mechanism for adding test tubes 100 and a sampling mechanism for pushing the test tubes 100 and sampling the test tubes 100 are provided in the isolation cylinder 5.

[0029] like Figure 3As shown, the driving rotation mechanism includes an inner gear ring 91, a rotating shaft 92, a driving motor 93 and a driving gear 94. The rotating bracket 6 is connected with the inner gear ring 91, and the isolating cylinder 5 is rotatably connected with the rotating shaft 92. Four driving gears 94 are evenly spaced and connected to the rotating shaft 92. The driving gears 94 are meshed with the inner gear ring 91 so that the rotation of the driving gear 94 can drive the inner gear ring 91 to rotate, thereby driving the rotating bracket 6 to rotate. A driving motor 93 is installed on the top of the tank cover 2, and the output shaft of the driving motor 93 is connected to the rotating shaft 92.

[0030] like Figure 4 and Figure 5 As shown, the adding mechanism includes a partition plate 101, a guide cylinder 102, a feed cylinder 103, a support tray 104, a servo motor 105 and a driving screw 106. The guide cylinder 102 is connected to the right side of the isolation cylinder 5, and the partition plate 101 is connected to the upper part of the isolation cylinder 5. The partition plate 101 is connected to the guide cylinder 102. When adding liquid nitrogen, it is added to the position below the partition plate 101. The feed cylinder 103 is slidably connected in the guide cylinder 102, and the support tray 104 is connected to the lower part of the feed cylinder 103. The feed cylinder 103 and the support tray 104 can support and transport the test tube 100. A servo motor 105 is installed on the top of the partition plate 101, and a driving screw 106 is connected to the output shaft of the servo motor 105. The driving screw 106 is threadedly matched with the feed cylinder 103, so that the rotation of the driving screw 106 can drive the feed cylinder 103 to move up and down through the thread.

[0031] like Figure 5-Figure 7 As shown, the sampling mechanism includes a sliding frame 111, a sliding rod 112, an elastic member 113, a sliding plate 114, a pushing rod 115, an extension shaft 116, a pushing arc plate 117 and an elastic member 2 118. The partition plate 101 and the left side of the tank cover 2 are both slidably connected with the sliding rod 112, the bottom of the sliding rod 112 is connected to the sliding frame 111, and an elastic member 113 is connected between the sliding rod 112 and the partition plate 101. The elastic member 113 is a connecting spring, and a plurality of sliding plates 114 are evenly spaced and connected to the sliding frame 111. The guide cylinder 102 There are four push rods 115 slidably connected at even intervals on the left side, and an extension shaft 116 is connected to the lower part of the push rod 115. The sliding plate 114 is provided with oblique grooves on both the front and rear sides, and the extension shaft 116 is located in the oblique grooves, so that when the sliding plate 114 moves, the extension shaft 116 can be squeezed through the oblique grooves to move. The right side of the push rod 115 is rotatably connected to two pushing arc plates 117, and the pushing arc plates 117 can clamp and push the test tube 100. An elastic member 118 is connected between the two pushing arc plates 117, and the elastic member 118 is a torsion spring.

[0032] When it is necessary to store biological gene samples, the device can be used. When in use, if it is necessary to store the samples, the locking nut 42 can be removed, and then the opening and closing cover 3 can be opened, and then the test tube 100 containing the sample can be placed in the feeding barrel 103, and the test tube 100 can be supported by the support tray 104, and then the servo motor 105 can be controlled to drive the driving screw 106 to rotate. When the driving screw 106 rotates, it can drive the feeding barrel 103 and the support tray 104 to move downward through the thread. When the support tray 104 drives the test tube 100 to align with the through-port 8, it can squeeze the sliding rod 112 to move downward, and the elastic member 113 is stretched. The downward movement of the sliding rod 112 can drive the sliding frame 111 to move downward, and the sliding frame 111 can drive the sliding plate 114 to move downward when it moves downward. When the sliding plate 114 moves downward, it can squeeze the extension shaft 116 to move to the right through the inclined groove. When the extension shaft 116 moves, it can drive the push rod 115 to move. When the push rod 115 moves, it can drive the push arc plate 117 to move to the right. When the push arc plate 117 moves to the right, it will first contact the test tube 100 so that the two push arc plates 117 are separated from each other and opened, and the elastic member 118 is deformed. Then, when the push arc plate 117 moves to the two sides of the test tube 100, the push arc plate 117 can clamp the test tube 100 under the action of the elastic member 118. Then, the push arc plate 117 continues to move to push the test tube 100, and the test tube 100 is pushed through the through port 8 to the card slot of the placement plate 7. Then, it can be controlled The driving motor 93 drives the rotating shaft 92 to rotate, and the rotation of the rotating shaft 92 drives the driving gear 94 to rotate. The rotation of the driving gear 94 drives the inner gear ring 91 to rotate, thereby driving the rotating bracket 6 and the placement plate 7 to rotate, so that the slot on the placement plate 7 where the test tube 100 is not placed is aligned with the through opening 8. When the placement plate 7 rotates, it also drives the test tube 100 to move, so that the test tube 100 squeezes the pushing arc plate 117 to swing and disengage from the pushing arc plate 117. After the placement is completed, the sliding rod 112 is released, and the sliding rod 112 and the sliding frame 111 are reset under the action of the elastic member 113. This is repeated, and the storage of biological gene samples can be realized. When extraction work is required, the servo motor 105 is first controlled to drive the driving screw 106 to rotate, and the screw belt The feeding barrel 103 and the tray 104 are moved downward so that the feeding barrel 103 is located on the left side of the test tube 100 to be extracted. At this time, the sliding rod 112 is pressed to drive the pushing arc plate 117 to move to the right, so that the test tube 100 at the slot can be grabbed. The two pushing arc plates 117 are brought close to clamp the test tube 100 through the elastic member 118. Then, the sliding rod 112 is released to reset the pushing arc plate 117 to put the test tube 100 into the position of the feeding barrel 103 and the tray 104. The servo motor 105 is controlled to drive the driving screw 106 to rotate, thereby driving the feeding barrel 103 and the tray 104 to move upward. During the upward movement, the liquid nitrogen falling on the tray 104 will be discharged through the opening 8 until the tray 104 moves up to above the uppermost opening 8.At this time, the cold air inside the entire storage tank 1 can be isolated, and then the opening and closing cover 3 is opened to take out the test tube 100, and the extraction of the test tube 100 is completed. In this way, the device can be used to extract the sample, and during the extraction process, the cold air can be isolated by the support tray 104, which can prevent the cold air from escaping and reduce the burden on the refrigeration system.

[0033] like Figure 8 and Fig. 9 As shown, a blocking mechanism is also included, and the blocking mechanism includes a blocking plate 121, a blocking plate 122 and an elastic member 123. A discharge port is opened in the middle of the supporting tray 104, and a blocking plate 122 is connected to the lower inner part of the feeding tube 103. The blocking plate 122 is located below the supporting tray 104. A blocking plate 121 is slidably connected to the blocking plate 122, and the blocking plate 121 blocks the discharge port. An elastic member 123 is connected between the blocking plate 121 and the blocking plate 122, and the elastic member 123 is a reset spring.

[0034] During the upward movement of the feeding cylinder 103 and the receiving tray 104, if the receiving tray 104 moves to above the uppermost through-port 8, the receiving tray 104 continues to move upward and will squeeze the gas on the guide cylinder 102 and the partition plate 101, so that this part of the gas squeezes the plugging plate 121 to move downward, and the elastic member 123 is compressed. After the plugging plate 121 moves downward, the discharge port will be exposed. At this time, if there is any liquid nitrogen remaining on the receiving tray 104, it can flow back into the storage tank 1 below the receiving tray 104 through the discharge port, so as to avoid liquid nitrogen remaining on the receiving tray 104.

[0035] like Figure 8 As shown, a limiting ring 13 is also included. The upper inner portion of the feeding cylinder 103 is connected with a limiting ring 13 for limiting the position of the test tube 100 to prevent the position of the test tube 100 from being offset.

[0036] like Fig.10 and Fig.11 As shown, it also includes a limiting mechanism, which includes a push rod 141, an elastic member 142 and a blocking ring 143. The push rod 141 is slidably connected between the tank cover 2 and the partition plate 101, and an elastic member 142 is connected between the push rod 141 and the tank cover 2. The elastic member 142 is a tension spring. Four blocking rings 143 are evenly spaced and connected to the push rod 141. The blocking ring 143 is located outside the isolation cylinder 5. The blocking ring 143 can block the test tube 100 to limit the test tube 100.

[0037] When adding the test tube 100, first pull the push rod 141 upward to drive the blocking ring 143 upward, the elastic member 142 is compressed, and the blocking ring 143 no longer blocks the test tube 100 after moving upward, and the test tube 100 can be added at this time. After the test tube 100 is added, release the push rod 141, and the push rod 141 and the blocking ring 143 are reset under the action of the elastic member 142, which can block the inside of the test tube 100 and prevent the test tube 100 from moving randomly, thereby limiting the position of the test tube 100.

[0038] Although the present disclosure has been shown and described with reference to specific exemplary embodiments of the present disclosure, it should be understood by those skilled in the art that various changes in form and details may be made to the present disclosure without departing from the spirit and scope of the present disclosure as defined by the appended claims and their equivalents. Therefore, the scope of the present disclosure should not be limited to the above-mentioned embodiments, but should be determined not only by the appended claims, but also by the equivalents of the appended claims.

Claims

1. A device for extracting biological gene samples, characterized in that: The invention comprises a storage tank body (1), a tank cover (2), an opening and closing cover plate (3), an isolation cylinder body (5), a rotating bracket (6), a placement plate (7), a driving rotating mechanism, an adding mechanism and a sampling mechanism. The top of the storage tank body (1) is connected to the tank cover (2), the top of the tank cover (2) is rotatably connected to the opening and closing cover plate (3), the bottom of the tank cover (2) is connected to the isolation cylinder body (5), and the outside of the isolation cylinder body (5) is evenly spaced and rotatably connected to four rotating brackets (6), and the lower part of each rotating bracket (6) is A placement plate (7) is connected, the placement plate (7) is provided with a plurality of slots for accommodating test tubes (100) at even intervals, the isolation cylinder (5) is provided with four openings (8) at even intervals, a driving rotation mechanism for driving the rotating bracket (6) to rotate is provided between the rotating bracket (6) and the tank cover (2), and an adding mechanism for adding test tubes (100) and a sampling mechanism for pushing the test tubes (100) and sampling the test tubes (100) are provided in the isolation cylinder (5).

2. A device for extracting biological gene samples as claimed in claim 1, characterized in that: It also comprises a threaded rod (41) and a locking nut (42); the tank cover (2) is connected with the threaded rod (41); the locking nut (42) can be screwed into the threaded rod (41) through a thread; the threaded rod (41) and the locking nut (42) are used to lock the opening and closing cover plate.

3. The device for extracting biological gene samples as claimed in claim 1, characterized in that: The driving rotating mechanism comprises an inner gear ring (91), a rotating shaft (92), a driving motor (93) and a driving gear (94); the rotating bracket (6) is connected with the inner gear ring (91); the isolating cylinder (5) is rotatably connected with the rotating shaft (92); four driving gears (94) are evenly spaced and connected to the rotating shaft (92); the driving gears (94) are meshed with the inner gear ring (91); the top of the tank cover (2) is equipped with a driving motor (93); the output shaft of the driving motor (93) is connected with the rotating shaft (92).

4. The device for extracting biological gene samples as claimed in claim 1, characterized in that: The adding mechanism comprises a partition plate (101), a guide cylinder (102), a feeding cylinder (103), a support tray (104), a servo motor (105) and a driving screw (106); the guide cylinder (102) is connected inside the isolation cylinder (5); the partition plate (101) is connected to the upper part of the isolation cylinder (5); the partition plate (101) is connected to the guide cylinder (102); the feeding cylinder (103) is slidably connected inside the guide cylinder (102); 03), a support tray (104) is connected to the lower inner part of the feeding barrel (103), the feeding barrel (103) and the support tray (104) can support the test tube (100) and transport the test tube (100), a servo motor (105) is installed on the top of the partition plate (101), and a driving screw (106) is connected to the output shaft of the servo motor (105), and the driving screw (106) is threadedly matched with the feeding barrel (103).

5. A device for extracting biological gene samples as claimed in claim 4, characterized in that: The sampling mechanism comprises a sliding frame (111), a sliding rod (112), an elastic member 1 (113), a sliding plate (114), a pushing rod (115), an extension shaft (116), a pushing arc plate (117) and an elastic member 2 (118). The partition plate (101) and the tank cover (2) are both slidably connected with the sliding rod (112). The bottom of the sliding rod (112) is connected to the sliding frame (111). The sliding rod (112) and the partition plate (101) are connected with the elastic member 1 (113). A plurality of sliding plates (114) are evenly spaced and connected to the sliding frame (111); four pushing rods (115) are evenly spaced and slidably connected to the guide cylinder (102); an extension shaft (116) is connected to the lower part of the pushing rod (115); oblique grooves are provided on both sides of the sliding plate (114); the extension shaft (116) is located in the oblique grooves; two pushing arc plates (117) are rotatably connected to the pushing rod (115); and a second elastic member (118) is connected between the two pushing arc plates (117).

6. The device for extracting biological gene samples as claimed in claim 4, characterized in that: The invention also comprises a blocking mechanism, wherein the blocking mechanism comprises a blocking plate (121), a blocking plate (122) and an elastic member three (123); a discharge port is provided in the middle of the support tray (104); a blocking plate (122) is connected to the lower part of the inner part of the feeding tube (103); the blocking plate (122) is located below the support tray (104); a blocking plate (121) is slidably connected to the blocking plate (122); and an elastic member three (123) is connected between the blocking plate (121) and the blocking plate (122).

7. The device for extracting biological gene samples as claimed in claim 4, characterized in that: It also comprises a limiting ring (13), and the upper inner part of the feeding cylinder (103) is connected with a limiting ring (13) for limiting the position of the test tube (100).

8. The device for extracting biological gene samples as claimed in claim 4, characterized in that: The invention also comprises a limiting mechanism, wherein the limiting mechanism comprises a push rod (141), four elastic members (142) and a blocking ring (143); the push rod (141) is slidably connected between the tank cover (2) and the partition plate (101); the four elastic members (142) are connected between the push rod (141) and the tank cover (2); four blocking rings (143) are evenly spaced and connected to the push rod (141); and the blocking rings (143) are located outside the isolation cylinder (5).

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