Composition for relieving cough and asthma

Abstract

The invention discloses a cough and asthma relieving composition, and belongs to the field of traditional Chinese medicines. The cough and asthma relieving composition is prepared from traditional Chinese medicine fermented particles, semen armeniacae amarae, folia perillae acutae and radix peucedani, the traditional Chinese medicine fermented particles are prepared from pericarpium citri reticulatae, radix platycodonis and radix glycyrrhizae as raw materials, cellulase is firstly used for conducting enzymolysis on the raw materials to obtain enzymatic hydrolysate, then lactobacillus plantarum, lactobacillus johnsonii and rhodotorula mucilaginosa are used for conducting mixed fermentation on the enzymatic hydrolysate to obtain fermentation liquor, and the fermentation liquor is prepared into the cough and asthma relieving composition. And sterilizing, filtering, drying and granulating the fermentation liquor to obtain the traditional Chinese medicine fermented granules. The traditional Chinese medicine fermented granules provided by the invention are compounded with the semen armeniacae amarae, the folium perillae and the radix peucedani for use, so that a synergistic effect is achieved, and an excellent cough and asthma relieving effect is achieved.

Description

Technical Field

[0001] The present application relates to the technical field of traditional Chinese medicine, and in particular to a cough and asthma relieving composition. Background Art

[0002] Xing Su San is composed of perilla leaves, pinellia, poria, peucedanum, bitter almond, bitter platycodon, fructus aurantii, tangerine peel, licorice, ginger and jujube. It is mainly used to treat exogenous cold and dryness syndrome, aversion to cold and no sweating, slight headache, cough with thin sputum, nasal congestion and dry throat, white tongue coating and stringy pulse. It is one of the ancient prescriptions recorded in Su Wen Zhi Zhen Yao Da Lun. Perilla leaves and bitter almond are the main ingredients, pinellia, platycodon and fructus aurantii are the secondary ingredients, pinellia, tangerine peel, poria, ginger and jujube are the auxiliary ingredients, and licorice is the assistant ingredient. It has certain cough, phlegm and asthma-relieving effects, but the decoction preparation of traditional Chinese medicine makes it impossible to take it in time when symptoms appear, and the decoction time and the degree of decoction may affect the efficacy, which is extremely inconvenient.

[0003] With the development of science and technology, fermentation technology is rapidly updated and iterated. The synergistic effect between multiple strains in multi-strain mixed fermentation technology is expected to further amplify the efficacy of the formula. In addition, compared with traditional Chinese medicine decoction, granule preparations have the advantages of convenient carrying, accurate dosage, strong stability, and long-term storage at room temperature. Therefore, it is of great research value to choose to use multi-strain mixed fermentation to improve the process of existing Chinese medicine formulas and use granules to replace traditional Chinese medicine dosage forms. Summary of the invention

[0004] The purpose of the present invention is to provide a cough and asthma relieving composition. The composition comprises the following steps: a multi-strain mixed fermentation method is used to mix and ferment tangerine peel, platycodon and licorice, and then the fermentation product is simply prepared with bitter almond, perilla leaf and peucedanum to obtain a composition capable of improving respiratory diseases. The composition has high safety, and the fermentation product obtained by fermenting tangerine peel, platycodon and licorice is used in combination with bitter almond, perilla leaf and peucedanum to achieve synergistic effect, and has excellent cough and asthma relieving effects.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a method for preparing Chinese medicine fermented particles, wherein the preparation raw materials include the following ingredients: dried tangerine peel, platycodon grandiflorum, and liquorice, and the mass ratio thereof is 1:1:0.3-0.4.

[0007] The specific preparation steps of the Chinese medicine fermented granules are as follows:

[0008] S1: Mix and grind dried orange peel, platycodon grandiflorum and liquorice, and pass through a 400-600 mesh sieve to obtain a mixed powder;

[0009] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0010] S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 5-7wt% of the mixed solution, the enzymolysis temperature is 30-35°C, the enzymolysis time is 3-6h, and after the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution;

[0011] S4: adding 9-12 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105° C. for 40 min, and filtering to obtain a filtrate;

[0012] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and then performing mixed fermentation, sterilizing at high temperature and filtering after the fermentation is completed to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0.5-1:1, the total amount of bacteria is 8-10wt% of the filtrate, the fermentation time is 12-16h, the fermentation temperature is 28-30°C, the fermentation pH is 5.8-7.1, and the ventilation volume is 3L / min;

[0013] S6: Concentrating the Chinese medicine fermentation liquid, spray drying and granulating to obtain Chinese medicine fermentation granules.

[0014] Preferably, the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosae is 1:0.7:1.

[0015] In a second aspect, the present invention provides a cough and asthma relieving composition, wherein the composition is made of the following components in parts by weight:

[0016] 5.4-5.6 parts of Chinese medicine fermented granules;

[0017] 1-1.3 portions of bitter almonds;

[0018] 1-1.3 parts of perilla leaves;

[0019] Peucedanum 1-1.3 parts;

[0020] Sucrose 90-92 parts.

[0021] Preferably, the composition is made of the following components in parts by weight:

[0022] 5.4-5.5 parts of Chinese medicine fermented granules;

[0023] Bitter almonds 1-1.15 parts;

[0024] 1-1.15 parts of perilla leaves;

[0025] Peucedanum 1-1.15 parts;

[0026] Sucrose 91-92 parts.

[0027] Most preferably, the composition is made from the following components in parts by weight:

[0028] 5.5 parts of Chinese herbal fermented granules;

[0029] Bitter almonds 1.15 parts;

[0030] 1.15 parts of perilla leaves;

[0031] Peucedanum 1.15 parts;

[0032] Sucrose 91.05 parts.

[0033] In a third aspect, the present invention provides a method for preparing an antitussive and antiasthmatic composition, the specific steps of which are as follows:

[0034] T1: Crush bitter almonds, soak them in water for 20-26 hours, steam distill, collect the distillate, add 1-2 v / v% 90-95 v / v% ethanol to the distillate, distill it again, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 ml, and seal it for later use;

[0035] T2: Extract the extracts of Perilla leaf and Peucedanum chinense by steam distillation, seal and set aside, wherein the solid-liquid ratio of the raw material to water is 1:20;

[0036] T3: dissolving the Chinese medicine fermented granules in the bitter almond secondary distillation dilution solution to obtain a mixed solution;

[0037] T4: uniformly mixing the perilla leaf extract, the peucedanum chinense extract, sucrose, and the mixed solution obtained in step T3, cooling, and spray-drying to obtain finished granules;

[0038] T5: Divide the finished granules into packages, seal them, and store them at room temperature.

[0039] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides a cough and asthma relieving composition, which is made of Chinese medicine fermented granules, bitter almonds, perilla leaves, and peucedanum, wherein the Chinese medicine fermented granules are made of tangerine peel, platycodon, and licorice, and the raw materials are first enzymolyzed with cellulase to obtain an enzymolysis solution, and then the enzymolysis solution is mixed and fermented with plant lactobacillus, lactobacillus johnsonii, and red yeast to obtain a fermentation liquid, and then the mixed fermentation liquid is sterilized, filtered, dried, and granulated to obtain the Chinese medicine fermented granules. Among them, the secondary metabolites of plant lactobacillus can improve the biological barrier and enhance immunity; Lactobacillus johnsonii and its secondary metabolites may be one of the bacteria involved in respiratory immune protection; yeast can cooperate with Lactobacillus strains for reproduction and nutritional complementation, promote the proliferation of lactobacillus, and carotenoids in red yeast can also help neutralize free radicals and reduce cell damage, thereby protecting respiratory cells and lung cells. The study found that the fermentation products obtained by mixed fermentation of tangerine peel, platycodon, and licorice with Lactobacillus plantarum, Lactobacillus johnsonii, and red yeast and their combination with bitter almonds, perilla leaves, and peucedanum has better antitussive and antiasthmatic effects than the simple combination of tangerine peel, platycodon, licorice with bitter almonds, perilla leaves, and peucedanum. DETAILED DESCRIPTION

[0040] In order to better understand the present invention, the present invention is further described below in conjunction with specific application examples, wherein the terms used in the application examples are for describing specific implementation schemes and do not constitute limitations on the protection scope of the present invention.

[0041] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the calculation is based on mass percentage and mass fraction.

[0042] Some raw materials and their sources are as follows:

[0043] Tangerine peel: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0044] Platycodon grandiflorum: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.;

[0045] Licorice: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0046] Bitter almonds: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0047] Perilla leaves: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0048] Peucedanum chinense: purchased from Bozhou Ruantang Pharmaceutical Co., Ltd.

[0049] Cellulase: purchased from Xiasheng Group, Xiasheng cellulase (CEL-01 model), product number: FDY-2243, enzyme activity: 11000u / mL;

[0050] Lactobacillus plantarum: purchased from China General Microorganism Collection Center, with the collection number CGMCC 1.568;

[0051] Lactobacillus johnsonii: purchased from China General Microorganism Collection Center, with the collection number CGMCC 1.10778;

[0052] Rhodotorula glutinosae: purchased from China General Microorganism Collection Center, with the collection number of CGMCC 2.5690.

[0053] The preparation process of Chinese medicine fermented granules, the specific steps are as follows:

[0054] Chinese herbal fermented granules 1:

[0055] S1: Mix and grind tangerine peel, platycodon grandiflorum and licorice, and pass through a 500-mesh sieve to obtain a mixed powder, wherein the mass ratio of tangerine peel, platycodon grandiflorum and licorice is 1:1:0.4;

[0056] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0057] S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 6 wt% of the mixed solution, the enzymolysis temperature is 33° C., the enzymolysis time is 5 h, and after the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution;

[0058] S4: adding 10 wt % glucose and 3 wt % peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105° C. for 40 min, and filtering to obtain a filtrate;

[0059] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and then performing mixed fermentation, sterilizing at high temperature and filtering after the fermentation is completed to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0.7:1, the total amount of bacteria is 9wt% of the filtrate, the fermentation time is 14h, the fermentation temperature is 29°C, the fermentation pH is 6.5, and the sterile air ventilation volume is 3L / min;

[0060] S6: Concentrate the Chinese medicine fermentation liquid to 20% of the original volume, spray dry and granulate to obtain Chinese medicine fermentation granules.

[0061] Chinese herbal fermented granules 2:

[0062] S1: Mix and grind dried tangerine peel, platycodon grandiflorum and licorice, and pass through a 400-mesh sieve to obtain a mixed powder, wherein the mass ratio of dried tangerine peel, platycodon grandiflorum and licorice is 1:1:0.3;

[0063] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0064] S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 7 wt % of the mixed solution, the enzymolysis time is 3 h, the enzymolysis temperature is 30° C., and after the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution;

[0065] S4: adding 12 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105°C for 40 min, and filtering to obtain a filtrate;

[0066] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and then performing mixed fermentation, sterilizing at high temperature and filtering after the fermentation is completed to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0.5:1, the total amount of bacteria is 8wt% of the filtrate, the fermentation time is 16h, the fermentation temperature is 28°C, the fermentation pH is 5.8, and the sterile air ventilation volume is 3L / min;

[0067] S6: Concentrate the Chinese medicine fermentation liquid to 30% of the original volume, spray dry and granulate to obtain Chinese medicine fermentation granules.

[0068] Chinese herbal fermented granules 3:

[0069] S1: Mix and grind dried tangerine peel, platycodon grandiflorum and licorice, and pass through a 600-mesh sieve to obtain a mixed powder, wherein the mass fractions of dried tangerine peel, platycodon grandiflorum and licorice are 1:1:0.4;

[0070] S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution;

[0071] S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 5wt% of the mixed solution, the enzymolysis time is 6h, the enzymolysis temperature is 35°C, and after the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution;

[0072] S4: adding 9 wt % glucose and 3 wt % peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105° C. for 40 min, and filtering to obtain a filtrate;

[0073] S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and then performing mixed fermentation, sterilizing at high temperature and filtering after the fermentation is completed to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:1:1, the total amount of bacteria is 10wt% of the filtrate, the fermentation time is 12h, the fermentation temperature is 30°C, the fermentation pH is 7.1, and the sterile air ventilation volume is 3L / min;

[0074] S6: Concentrate the Chinese medicine fermentation liquid to 10% of the original volume, spray dry and granulate to obtain Chinese medicine fermentation granules.

[0075] Chinese medicine fermented granules①:

[0076] The difference from the Chinese medicine fermented granules 1 is that the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0:1, and the remaining steps are the same as those of the Chinese medicine fermented granules 1.

[0077] Chinese medicine fermented granules②:

[0078] Different from the Chinese medicine fermented granules 1, the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0.7:0, and the remaining steps are the same as those of the Chinese medicine fermented granules 1.

[0079] Chinese medicine fermented granules ③:

[0080] Different from the Chinese medicine fermented granules 1, the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 0:0.7:1, and the remaining steps are the same as those of the Chinese medicine fermented granules 1.

[0081] Chinese medicine fermented granules④:

[0082] Different from the Chinese medicine fermentation granules 1, the steps S4-S5 are not included, the enzymatic hydrolysate obtained in the step S3 is subjected to the step S6, and the remaining steps are the same as the Chinese medicine fermentation granules 1.

[0083] Chinese medicine fermented granules⑤:

[0084] Different from the Chinese medicine fermentation granules 1, it does not include steps S3-S5, and the mixed solution obtained in step S2 is subjected to hot reflux extraction, the extraction temperature is 65°C, the extraction time is 5h, the extraction is performed 4 times, the filtrates are combined, and then step S6 is performed on the filtrates.

[0085] Chinese medicine fermented granules⑥:

[0086] Different from the Chinese medicine fermentation granules 1, the S3 enzymatic hydrolysis step is not included, the mixed liquid obtained in S2 is directly subjected to the S4 step, and the remaining steps are the same as the Chinese medicine fermentation granules 1.

[0087] Chinese medicine fermented granules⑦:

[0088] The difference from the Chinese medicine fermentation granules 1 is that only red yeast is used, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0089] Chinese medicine fermented granules⑧:

[0090] Different from the Chinese medicine fermented granules 1, only Lactobacillus johnsonii is used, and the remaining steps are the same as those of the Chinese medicine fermented granules 1.

[0091] Chinese medicine fermented granules ⑨:

[0092] Different from the Chinese medicine fermented granules 1, only Lactobacillus plantarum is used, and the remaining steps are the same as those of the Chinese medicine fermented granules 1.

[0093] Traditional Chinese medicine fermented granules ⑩:

[0094] Different from the Chinese medicine fermentation granules 1, the fermentation time of step S5 is 96 hours, and the remaining steps are the same as those of the Chinese medicine fermentation granules 1.

[0095] A cough and asthma relieving composition, the weight proportion of which is shown in Table 1:

[0096] Table 1 Composition mass ratio

[0097]

[0098]

[0099]

[0100] Composition The missing components were replaced with equal amounts of sterile deionized water.

[0101] The preparation methods of the above compositions 1, 4, 5 and compositions ①-⑩ are as follows:

[0102] T1: Crush bitter almonds, soak them in water for 24 hours, with a solid-liquid ratio of 1:20, perform steam distillation at normal pressure, collect the distillate, add 2v / v% 93v / v% ethanol to the distillate, perform secondary distillation, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3mg hydrocyanic acid per 1mL, and seal for later use;

[0103] T2: Extracting the extracts of Perilla leaf and Peucedanum chinense by steam distillation, sealing and setting aside, wherein the ratio of raw material to water is 1:20, and the pressure is normal pressure;

[0104] T3: dissolving the Chinese medicine fermented granules in the bitter almond secondary distillate to obtain solution B;

[0105] T4: uniformly mixing the perilla leaf extract, the peucedanum chinense extract, sucrose and solution B, cooling, and spray-drying to obtain granules B;

[0106] T5: Divide granules B into smaller packages, seal them, and store them at room temperature.

[0107] The preparation method of composition 2 is as follows:

[0108] T1: Crush bitter almonds, soak them in water for 20 hours, with a solid-liquid ratio of 1:20, and perform steam distillation at normal pressure. Collect the distillate, add 1v / v% of 95v / v% ethanol to the distillate, perform secondary distillation, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3mg of hydrocyanic acid per 1mL, and seal for later use;

[0109] T2: Extracting the extracts of Perilla frutescens leaves and Peucedanum peucedanum by steam distillation, sealing and setting aside, wherein the ratio of raw materials to water is 1:20, and the pressure is normal pressure;

[0110] T3: dissolving the Chinese medicine fermented granules in the bitter almond secondary distillate to obtain solution B;

[0111] T4: uniformly mixing the perilla leaf extract, the peucedanum chinense extract, sucrose and solution B, cooling, and spray-drying to obtain granules B;

[0112] T5: Divide granules B into smaller packages, seal them, and store them at room temperature.

[0113] The preparation method of composition 3 is as follows:

[0114] T1: Crush bitter almonds, soak them in water for 26 hours, with a solid-liquid ratio of 1:20, and perform steam distillation at normal pressure. Collect the distillate, add 2v / v% of 90v / v% ethanol to the distillate, perform secondary distillation, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3mg of hydrocyanic acid per 1mL, and seal for later use;

[0115] T2: Extracting the extracts of Perilla frutescens leaves and Peucedanum peucedanum by steam distillation, sealing and setting aside, wherein the ratio of raw materials to water is 1:20, and the pressure is normal pressure;

[0116] T3: dissolving the Chinese medicine fermented granules in the bitter almond secondary distillate to obtain solution B;

[0117] T4: uniformly mixing the perilla leaf extract, the peucedanum chinense extract, sucrose and solution B, cooling, and spray-drying to obtain granules B;

[0118] T5: Divide granules B into smaller packages, seal them, and store them at room temperature.

[0119] Composition

[0120] T1: Crush bitter almonds, soak them in water for 24 hours, with a solid-liquid ratio of 1:20, perform steam distillation at normal pressure, collect the distillate, add 2v / v% 93v / v% ethanol to the distillate, perform secondary distillation, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3mg hydrocyanic acid per 1mL, and seal for later use;

[0121] T2: Extracting the extracts of Perilla frutescens leaves and Peucedanum peucedanum by steam distillation, sealing and setting aside, wherein the ratio of raw materials to water is 1:20, and the pressure is normal pressure;

[0122] T3: Evenly mix the perilla leaf extract, peucedanum chinense extract, sucrose and bitter almond secondary distillate, cool, and spray-dry to obtain granules B;

[0123] T4: Divide granules B into smaller packages, seal them, and store them at room temperature.

[0124] Composition

[0125] T1: dissolving the fermented Chinese medicine granules in distilled water to obtain solution A;

[0126] T2: Mix sucrose and solution A evenly, cool, and spray-dry to obtain granules A;

[0127] T3: Divide granules A into smaller packages, seal them, and store them at room temperature.

[0128] Composition A:

[0129] The difference from the preparation method of composition 1 is that one distillation is used in step T1, and the remaining steps are the same as those of composition 1.

[0130] Composition B:

[0131] The difference from the preparation method of composition 1 is that in step T1, 93v / v% ethanol reflux extraction is used instead of steam distillation, the solid-liquid ratio is 1:20, the ethanol reflux extraction temperature is 65°C, the reflux times are 2 times, and after reflux, the secondary reflux liquid is diluted with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL, and the mixture is sealed for later use; in step T2, 93v / v% ethanol reflux extraction is used instead of steam distillation, the solid-liquid ratio is 1:20, the ethanol reflux extraction temperature is 65°C, and the reflux times are 1 time; steps T1-T2 are all under normal pressure; and the remaining steps are the same as composition 1.

[0132] Test 1: Cough test

[0133] Test materials: Ammonia water (Guangdong Chemical Reagent Engineering Research and Development Center), codeine phosphate (Qinghai Pharmaceutical Co., Ltd.).

[0134] Experimental animals: KM mice (half male and half female), weighing 24±4 g, SPF grade; purchased from Guangdong Weitonglihua Experimental Animal Technology Co., Ltd.

[0135] Test instrument: Ultrasonic atomizer (purchased from Yuehua WH-2000).

[0136] Experimental methods:

[0137] (1) Animal screening

[0138] After the hot ultrasonic atomizer, add 40mL of freshly prepared ammonia solution (12.5wt%). Put the mouse into a beaker with a volume of 1000mL, seal the beaker mouth with plastic wrap, and open two openings (one for inserting the ultrasonic atomizer outlet pipe, and the other for ventilation holes). After the ultrasonic atomizer is continuously passed with ammonia for 60s at the minimum spray intensity, immediately close and quickly open the plastic wrap to allow the ammonia to diffuse naturally. While passing ammonia, use a stopwatch to time and record the mouse cough latency (s) and the number of coughs (times) within three minutes. The manifestation of coughing is: coughing when opening the mouth wide or small, and abdominal muscle contraction can be seen.

[0139] Elimination criteria for mouse cough sensitivity: ① Coughing less than 10 times and more than 80 times within three minutes; ② The incubation period after spraying is less than 15 seconds and more than 50 seconds; ③ Others: animal death, poor condition, abnormal reaction, etc.; Animals that meet any of the three conditions will be eliminated and will not be included in subsequent experiments.

[0140] (2) Effect of the composition on the mouse cough model induced by concentrated ammonia

[0141] 210 mice (113 females and 107 males) were screened out of 240 mice, and the qualified animals were randomly divided into a control group, a positive group (codeine phosphate; dosage: 15 mg / kg), and a test group (compositions 1-5, compositions ①- , composition A, composition B; dosage: 35 mg / kg); 10 mice in each group, the drug group (including the positive group and the test group) was gavaged with the corresponding drug solution (20 mL / kg, the solvent was normal saline), and the control group was gavaged with normal saline (20 mL / kg), and the drug was administered 3 times, once a day. 1 hour after the last administration, 12.5wt% ammonia water was used to stimulate the mice, and the cough latency (s) and the number of coughs within three minutes (times) were recorded. Statistical method: Data are expressed as mean ± standard deviation The statistical analysis was performed using SPSS 17.0 statistical analysis software, and one-way analysis of variance was used for analysis and statistics. P < 0.05 was considered statistically significant.

[0142] Test results: After three administrations, compositions 1-5 all tended to prolong the cough latency period, but there was a significant difference compared with the control group; compositions 1-5 and the positive group (codeine phosphate) significantly reduced the number of coughs within 3 minutes, which was significantly different from the control group.

[0143] Table 2 Effects of three doses of medication on the latency period (s) and the number of coughs (times) within three minutes of ammonia-induced cough in mice

[0144]

[0145]

[0146] Note: Compared with the control group, *P<0.05, **P<0.01.

[0147] Conclusion: In the animal model, compositions 1-5 (35 mg / kg) all had significant antitussive effects, and composition 1 had a similar effect to codeine phosphate.

[0148] Experiment 2: Antiasthmatic effect experiment

[0149] Test reagents: histamine phosphate, aminophylline solution.

[0150] Experimental animals: 147 SPF guinea pigs (74 females and 73 males), weighing 450±50 g, were purchased from Guangdong Weitonglihua Experimental Animal Technology Co., Ltd.

[0151] Main instruments: Ultrasonic nebulizer (purchased from Yuehua WH-2000).

[0152] Test method: Guinea pigs were randomly divided into a control group, a positive group (aminophylline solution, concentration of 12.5wt%) and a test group (compositions 1-5, compositions ①- , composition A, composition B, dosage: 35mg / kg), 7 in each group. The drug administration group (including the positive group and the test group) was intragastrically administered with the corresponding drug solution (20mL / kg, the solvent was normal saline), and the control group was intragastrically administered with normal saline (20mL / kg), and the drug administration was once a day for 3 consecutive days. One hour after the last administration, the guinea pigs were allowed to inhale 0.4v / v% histamine phosphate, and the time when the guinea pigs convulsed and fell was recorded as the latent period (the time from the start of spraying to the fall), and the observation was continued for 6min, and those who did not fall within 6min were counted as 360s.

[0153] Statistical method: Data are presented as mean ± standard deviation The statistical analysis was performed using SPSS 17.0 statistical analysis software, and one-way analysis of variance was used for analysis and statistics. P < 0.05 was considered statistically significant.

[0154] Test results: After three days of administration, the latent period of asthma induction in Compositions 1-5 and the positive group (aminophylline solution) was significantly longer than that in the control group and Compositions ①- , which was significantly different compared with the control group.

[0155] Table 3 Effects of 3-day drug administration on the latency period (s) of histamine-induced asthma in mice

[0156]

[0157]

[0158] Note: Compared with the control group, *P<0.05, **P<0.01.

[0159] Conclusion: In the experimental animal model, compositions 1-5 (dosage: 35 mg / kg) all have significant antiasthmatic effects, and composition 1 has a similar effect to aminophylline.

[0160] Test 3: Hepato-renal toxicity test

[0161] Test reagent: urethane.

[0162] Experimental animals: 60 KM mice (male), weighing 18±3 g, SPF grade, purchased from Guangdong Weitonglihua Experimental Animal Technology Co., Ltd.

[0163] Main instrument: optical microscope.

[0164] Test method: Mice were randomly divided into a control group and a test group (compositions 1-5, composition ①- , Composition A, Composition; Dosage: 35mg / kg), 10 mice in each group. The drug administration group (including the positive group and the test group) was intragastrically administered with the corresponding drug solution (20mL / kg, the solvent was saline), and the control group was intragastrically administered with saline (20mL / kg), and the drug administration was continued for 7 days. The weight was weighed and recorded before administration, and the mice were fasted for 12 hours before being killed. The weight was weighed, and 15wt% urethane was injected intraperitoneally for anesthesia. Blood was collected from the abdominal aorta, and the serum was separated. The four biochemical indicators of seramine aminotransferase (ALT), aspartate aminotransferase (AST), urea nitrogen (BUN), and creatinine (Cr) of mice in each group were determined. The mice were killed, and the liver and kidney were taken to measure the liver coefficient and kidney coefficient respectively. The liver coefficient = liver mass / physique × 100%, and the kidney coefficient = kidney mass / physique × 100%. The liver and kidney tissues were taken, fixed with 4v / v% formaldehyde solution, dehydrated routinely, embedded in paraffin, sliced, and stained with HE. The microstructure of the liver and kidney tissues was observed under a light microscope.

[0165] Statistical method: Data are presented as mean ± standard deviation The statistical analysis was performed using SPSS 17.0 statistical analysis software, and one-way analysis of variance was used for analysis and statistics. P < 0.05 was considered statistically significant.

[0166] Table 4 Effects of the composition on liver and kidney coefficients of mice

[0167]

[0168]

[0169] Note: Compared with the control group, *P<0.05.

[0170] Conclusion: The pathological sections of compositions 1-5 were basically similar to those of the control group. No obvious degeneration, necrosis or pathological abnormalities were observed in the liver and kidney cells. All indexes were basically similar to those of the control group, with no significant differences.

[0171] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. A method for preparing Chinese medicine fermented granules, characterized in that: The method comprises the following steps: enzymolyzing raw materials with cellulase to obtain enzymolysis solution; mixing and fermenting the enzymolysis solution with Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosae to obtain fermentation solution; sterilizing, filtering, concentrating and granulating the mixed fermentation solution to obtain the traditional Chinese medicine fermentation granules; wherein the raw materials are dried tangerine peel, platycodon grandiflorum and liquorice in a mass fraction ratio of 1:1:0.3-0.4, the number of the Lactobacillus johnsonii is CGMCC 1.10778, the number of the Lactobacillus plantarum is CGMCC 1.568, and the number of the Rhodotorula glutinosae is CGMCC 2.5690.

2. The method for preparing the Chinese medicine fermented granules according to claim 1, characterized in that: The specific steps of the preparation method are as follows: S1: Mix and grind dried orange peel, platycodon grandiflorum and liquorice, and pass through a 400-600 mesh sieve to obtain a mixed powder; S2: Add sterile deionized water 10 times the mass of the mixed powder to the mixed powder, stir evenly to obtain a mixed solution; S3: adding cellulase to the mixed solution for enzymolysis, wherein the amount of cellulase added is 5-7wt% of the mixed solution, the enzymolysis temperature is 30-35°C, the enzymolysis time is 3-6h, and after the enzymolysis is completed, filtering is performed to obtain an enzymolysis solution; S4: adding 9-12 wt% glucose and 3 wt% peptone to the enzymatic hydrolysate, mixing well, sterilizing the enzymatic hydrolysate at 105° C. for 40 min, and filtering to obtain a filtrate; S5: inoculating Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus into the filtrate and then performing mixed fermentation, sterilizing at high temperature and filtering after the fermentation is completed to obtain a Chinese medicine fermentation liquid, wherein the mass ratio of Lactobacillus plantarum, Lactobacillus johnsonii and Rhodotorula glutinosus is 1:0.5-1:1, the total amount of bacteria is 8-10wt% of the filtrate, the fermentation time is 12-16h, the fermentation temperature is 28-30°C, the fermentation pH is 5.8-7.1, and the sterile air ventilation volume is 3L / min; S6: Concentrating the Chinese medicine fermentation liquid, spray drying and granulating to obtain Chinese medicine fermentation granules.

3. A cough and asthma relieving composition, characterized in that: The invention comprises the fermented Chinese medicine particles as claimed in claim 1 or 2.

4. The antitussive and antiasthmatic composition according to claim 3, characterized in that: The antitussive and antiasthmatic composition is made of the following components in parts by weight: 5.4-5.6 parts of Chinese medicine fermented granules; 1-1.3 portions of bitter almonds; 1-1.3 parts of perilla leaves; Peucedanum 1-1.3 parts; Sucrose 90-92 parts.

5. The antitussive and antiasthmatic composition according to claim 4, characterized in that: The antitussive and antiasthmatic composition is made of the following components in parts by weight: 5.4-5.5 parts of Chinese medicine fermented granules; Bitter almonds 1-1.15 parts; 1-1.15 parts of perilla leaves; Peucedanum 1-1.15 parts; Sucrose 91-92 parts.

6. The antitussive and antiasthmatic composition according to claim 4, characterized in that: The antitussive and antiasthmatic composition is made of the following components in parts by weight: 5.5 parts of Chinese herbal fermented granules; Bitter almonds 1.15 parts; 1.15 parts of perilla leaves; Peucedanum 1.15 parts; Sucrose 91.05 parts.

7. A method for preparing the antitussive and antiasthmatic composition according to any one of claims 3 to 6, characterized in that: The steps include: T1: Crush bitter almonds, soak them in water for 20-26 hours, steam distill, collect the distillate, add 1-2 v / v% 90-95 v / v% ethanol to the distillate, distill it twice, collect the secondary distillate, dilute the secondary distillate with ultrapure water to a concentration of 0.3 mg of hydrocyanic acid per 1 mL, and seal it for later use; T2: Extract the extracts of Perilla frutescens leaves and Peucedanum peucedanum by steam distillation and seal them for later use; T3: dissolving the Chinese medicine fermented granules in the bitter almond secondary distillation dilution solution to obtain a mixed solution; T4: uniformly mixing the perilla leaf extract, the peucedanum chinense extract, sucrose, and the mixed solution obtained in step T3, cooling, and spray-drying to obtain finished granules; T5: Divide the finished granules into packages, seal them, and store them at room temperature.

Images