Device for producing bacterial cellulose composite material

Abstract

The invention discloses a device for producing a bacterial cellulose composite material, and relates to the technical field of bacterial cellulose production.The device comprises a culture medium container, rolling shafts, a framework material, a feeding rotary drum, a collecting rotary drum, a guide roller and a pressing roller, the rolling shafts are arranged in the culture medium container in two parallel rows, and the rolling shafts in each row are arranged in parallel at intervals; the two rows of rolling shafts are staggered in parallel; a framework material is fed by the feeding rotary drum and guided by the guide roller to enter the culture medium container, then is wound in a staggered manner along the two rows of rolling shafts, is conveyed in the culture medium container in an S-shaped track through rolling of the rolling shafts, and finally is pulled out of the culture medium container, penetrates through the pressing roller and is guided to enter the collecting rotary drum to be wound and collected. According to the method, efficient, continuous and large-scale production of the bacterial cellulose composite material is achieved, the product composite quality is improved, culture medium thallus breeding is accelerated, and the utilization efficiency of the culture medium is improved.

Description

[0001] This application is a divisional application of the invention patent whose parent application name is "A device for producing bacterial cellulose composite materials"; the application number of the parent application is: CN201710622263.X; the application date of the parent application is: 2017.07.27. Technical Field

[0002] The present application relates to the technical field of bacterial cellulose production, and in particular to a device for producing bacterial cellulose composite materials. Background Art

[0003] Bacterial cellulose (BC) is a type of pure cellulose produced by microorganisms, sometimes commonly known as "coconut fiber fruit", which can be widely used in food, facial masks, high-strength functional materials, textiles, papermaking, artificial blood vessels, tissue engineering scaffolds, artificial skin, and medical dressings. However, the existing shallow tray fermentation production equipment has low production efficiency and output, low mechanization, high labor intensity, and cannot be produced continuously. Therefore, it is urgent to develop a new type of fermentation device with high output and efficient continuous production.

[0004] In terms of medical dressings, wet bacterial cellulose is prone to breakage during use due to its insufficient mechanical strength, causing inconvenience in use. If it is to achieve the same mechanical properties as absorbent gauze, the cellulose film is required to reach a certain thickness, which will increase the hardness of the film and significantly reduce its skin adhesion.

[0005] The existing preparation methods of bacterial cellulose membrane composite materials all utilize static culture technology, including placing the materials to be composited with bacterial cellulose in culture medium for co-cultivation, and composite the bacterial cellulose membrane with organic polymers or inorganic substances by immersion, which is not efficient. Moreover, since cellulose is only formed on the surface of the culture medium, the amount of cellulose composited by the static culture method is small and unevenly distributed, and cannot cover most of the gaps in the materials to be composited, such as gauze, and the culture cycle is very long. Summary of the invention

[0006] The purpose of the present invention is to provide a device for producing bacterial cellulose composite materials to solve the problems existing in the above-mentioned prior art, realize the efficient, continuous and large-scale production of bacterial cellulose composite materials, improve the composite quality of products, accelerate the reproduction of culture medium bacteria and improve the utilization efficiency of culture medium.

[0007] The present invention adopts the following technical scheme: the present invention provides a device for producing bacterial cellulose composite materials, comprising a culture medium container, a rolling shaft, a skeleton material, a feeding drum, a collecting drum, a guide roller and a pressing roller, wherein the rolling shafts are arranged in two parallel rows inside the culture medium container, the rolling shafts in each row are arranged in parallel and spaced apart, and the rolling shafts in the two rows are parallel and staggered with each other, the skeleton material is first fed by the feeding drum and guided by the guide roller into the culture medium container, then staggered along the two rows of rolling shafts and rolled by the rolling shafts to be transported in the culture medium container in an S-shaped track, and finally pulled out of the culture medium container, passed through the pressing roller and guided into the collecting drum for winding and collection;

[0008] After passing through the pressing roller, the skeleton material can be guided back to the starting rolling shaft through the roller, forming a closed loop for cyclic production, until the bacterial cellulose is successfully attached to the skeleton material, and then guided into the collecting drum for winding and collection;

[0009] The side wall of the culture medium container is provided with a vent and a dissolved oxygen electrode socket, and a dissolved oxygen electrode is installed in the dissolved oxygen electrode socket;

[0010] The rolling shafts are arranged in two horizontally parallel rows inside the culture medium container, the skeleton material is staggered in the vertical direction and wound around the rolling shafts staggered in the vertical direction, and the liquid level of the culture medium container is controlled between the upper and lower rows of rolling shafts; or

[0011] The rolling shafts are arranged in two vertically parallel rows inside the culture medium container, and the skeleton material is horizontally staggered around the rolling shafts staggered on the left and right. The rolling shafts in the upper part of the two rows of rolling shafts are higher than the liquid level of the feed liquid.

[0012] In one embodiment, the culture medium container is provided with a heating plate inside and a liquid discharge port at the bottom, the side wall of the culture medium container is provided with a pH meter socket, a thermometer socket, an acid and alkali solution addition port, a nutrient feed port, a liquid height probe shaft and a feed port, a pH electrode is installed in the pH meter socket, a thermometer probe is installed in the thermometer socket, and a liquid level detection probe is installed in the culture medium container.

[0013] In one embodiment, the feeding drum and the collecting drum are respectively disposed on two sides of the culture medium container, and the guide roller and the pressure roller are correspondingly disposed on two opposite sides of the culture medium container and located at the top edge of the culture medium container.

[0014] In one embodiment, the skeleton material is a membrane material or a woven fabric material with a mesh, and the pore size of the mesh is 0.05 mm-10 mm.

[0015] In one embodiment, the culture medium container is a closed container or an open-top container.

[0016] In one embodiment, the linear speed of the skeleton material conveying is 10-280 m / h.

[0017] Beneficial effects of the present invention:

[0018] First, the skeleton material is sent and received by the feeding drum and the collecting drum, and is interlaced and strung together in an S-shaped track inside the culture medium container through the rolling shaft. By utilizing the easy winding property of bacterial cellulose, the skeleton material is used to wind and absorb the bacterial cellulose through the reciprocating movement of the skeleton material, so that a uniform and firmly bonded bacterial cellulose film can be formed on both sides of the skeleton material, and the obtained product is of high quality.

[0019] Second, it realizes the automated and continuous production of the composite of skeleton material and bacterial cellulose, improves production efficiency, reduces labor intensity, simplifies equipment, reduces production costs, and is conducive to large-scale industrial production;

[0020] Third, by controlling the transmission speed of the rolling shaft, it is possible to achieve sub-static culture of the bacteria in the culture medium container. The skeleton material can be constantly alternated between the liquid culture medium and the air, which can provide sufficient dissolved oxygen to the liquid culture medium, promote the rapid reproduction of the bacteria in the culture medium and produce cellulose, thereby facilitating the contact and entanglement of the skeleton material with the bacterial cellulose; at the same time, it is beneficial for the bacteria fixed on the skeleton material to be exposed to both nutrients and oxygen, thereby promoting the large-scale reproduction of the bacteria and the secretion of cellulose, and efficiently preparing bacterial cellulose materials. Various factors have greatly improved the production efficiency of bacterial cellulose materials;

[0021] Fourth, the skeleton material is arranged in a continuous S-shape inside the culture medium container, which makes full use of the space of the culture medium container, greatly increases the production area of ​​the bacterial cellulose composite material, improves the efficiency of the culture medium, and is conducive to increasing the yield;

[0022] Fifth, bacterial cellulose composite materials are prepared by combining skeleton materials with bacterial cellulose. The prepared bacterial cellulose membrane composite materials retain the unique nano three-dimensional network structure of bacterial cellulose, while saving the amount of bacterial cellulose. They have the advantages of high water absorption and water holding capacity, high crystallinity, high degree of polymerization, high strength and good biocompatibility. They can solve the problems of poor liquid absorption and adhesion of wounds of traditional dressings. The products can be widely used in food, facial masks, wound dressings, artificial skin, textile and paper raw materials and other fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 It is a front structural schematic diagram of an embodiment of the present invention in which rolling shafts are arranged in horizontal rows and the culture medium container is open.

[0025] Figure 2 for Figure 1 Side structural diagram of the rolling axis arrangement.

[0026] Figure 3 for Figure 1 Schematic diagram of the top view of the rolling axis arrangement.

[0027] Figure 4 It is a schematic structural diagram of an embodiment of the present invention in which rolling shafts are arranged horizontally in a row and a culture medium container is closed.

[0028] Figure 5 It is a schematic structural diagram of an embodiment of the present invention in which rolling shafts are arranged in vertical rows and culture medium containers are open.

[0029] Figure 6 for Figure 5 Side structural diagram of the rolling axis arrangement.

[0030] Figure 7 for Figure 5 Schematic diagram of the top view of the rolling axis arrangement.

[0031] Figure 8 It is a schematic structural diagram of an embodiment of the present invention in which rolling shafts are arranged in a vertical row and culture medium containers are closed.

[0032] Fig. 9 It is a schematic diagram of the structure in which the rolling shafts of the present invention are arranged horizontally in a row and the skeleton material forms a closed loop. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] like Figure 1-9The device shown in the figure for producing a bacterial cellulose composite material comprises a culture medium container 1, a rolling shaft 11, a skeleton material 13, a feeding drum 15, a collecting drum 16, a guide roller 17 and a pressing roller 10.

[0035] The culture medium container 1 is a closed rectangular parallelepiped or an open rectangular parallelepiped structure, and the open container is placed in a clean workshop of 10,000-100,000 levels. A heating plate 2 is provided inside the culture medium container 1, and a liquid discharge port 14 is provided at the bottom. The side wall of the culture medium container 1 is provided with a vent 3, a pH meter socket 4, a dissolved oxygen electrode socket 5, a thermometer socket 6, an acid and alkali solution addition port 7, a nutrient feed port 8, a liquid height probe shaft 9 and a feed port 12. A pH electrode is installed in the pH meter socket 4, a dissolved oxygen electrode is installed in the dissolved oxygen electrode socket 5, a thermometer probe is installed in the thermometer socket 6, and a liquid level detection probe is installed inside the culture medium container 1.

[0036] The feeding drum 15 and the collecting drum 16 are respectively arranged on two sides of the culture medium container 1 , and the guide roller 17 and the pressing roller 10 are correspondingly arranged on two sides of the culture medium container 1 and located at the top edge of the culture medium container 1 .

[0037] The rolling shafts 11 are arranged in two parallel rows inside the culture medium container 1, and can be installed through the side wall of the culture medium container 1. The open container can also be hung outside the container. The rolling shafts 11 in each row are arranged in parallel and spaced apart, and the rolling shafts 11 in the two rows are parallel and offset to each other. A preferred arrangement is as follows: the rolling shafts 11 are arranged in two rows vertically parallel to each other inside the culture medium container 1, the liquid level of the culture medium container 1 is controlled between the upper and lower rows of rolling shafts 11, the skeleton material 13 is fed by the feeding drum 15 and guided by the guide roller 17 to enter the culture medium container 1, is vertically staggered and wound along the two rows of rolling shafts 11, and is transported in the culture medium container 1 in an S-shaped trajectory by rolling through the rolling shafts 11, is pulled out of the culture medium container 1, passes through the pressing roller 10, and is guided into the collecting drum 16 for winding and collection; another preferred arrangement is as follows: the rolling shafts 11 are arranged in two rows vertically parallel to each other inside the culture medium container 1, the rolling shafts 11 in the upper part of the two rows of rolling shafts 11 are higher than the liquid level of the culture medium, the skeleton material 13 is fed by the feeding drum 15 and guided by the guide roller 17 to enter the culture medium container 1, is horizontally staggered and wound along the two rows of rolling shafts 11, and is transported in the culture medium container 1 in an S-shaped trajectory by rolling through the rolling shafts 11, is pulled out of the culture medium container 1, passes through the pressing roller 10, and is guided into the collecting drum 16 for winding and collection.

[0038] The skeleton material 13 is composed of organic and / or inorganic substances, including one or a combination of polysaccharides, cellulose, cellulose derivatives, agar, alginic acid, hyaluronic acid, chitin, chitosan, chitosan derivatives, wood, starch, polypeptides, proteins, gelatin, collagen, silk, wool, polyglutamic acid, high molecular polymers, silica gel, rubber, plastics, polyester, polypropylene, nylon, orlon, polyethylene, polyvinyl alcohol, polyvinyl ethanol, polytetrafluoroethylene, polyacrylamide, gauze, cotton, paper, silicon dioxide, glass, ceramics, purple sand, metal, metal salt, and metal oxide. The skeleton material 13 is a membrane material or a textile material with a mesh, the mesh aperture size is 0.05mm-10mm, and the shape can be round, square, triangular, elliptical, heart-shaped, plum blossom-shaped, diamond-shaped or five-pointed star-shaped. The linear speed of the skeleton material 13 is controlled to be 10-280m / h.

[0039] The specific embodiments are as follows:

[0040] Example 1

[0041] like Figure 1-3 As shown, the culture medium container 1 is an open rectangular container, which is 200 meters long, 2 meters wide and 3 meters high. There are 1000 parallel rolling shafts 11 in the upper and lower rows of the container. The distance between the upper and lower axes of the rolling shafts 11 is 2.5 meters, the distance between the left and right axes of the rolling shafts 11 is 0.2 meters, and the diameter of the rollers is 0.1 meters.

[0042] (1) Bacterial culture

[0043] Acetobacter xylinum was inoculated into 300 mL of liquid culture medium (400 g of glucose, 60 g of yeast extract, 100 g of tryptone, 1.15 g of citric acid, 2.7 g of Na2HPO4, pH 5.0, sterilized at 115°C for 30 min) and cultured by shaking at 30°C and 160 r / min or by static culture for 12 h for later use;

[0044] (2) Fermentation preparation of bacterial cellulose composite membrane

[0045] When using polyester fiber as the skeleton material 13, first inject the nutrient solution sterilized by high temperature steam into the culture medium container 1 from the feed port 12, then transfer the liquid culture medium containing the production strain into the culture medium container 1, and then drive the skeleton material 13 to perform disturbance culture in the culture medium at a speed of 200m / h by the motor, so that a bacterial cellulose film composite material of a certain thickness can be continuously and evenly formed on the surface of the skeleton material 13. Determine the appropriate speed, and when the film thickness meets the requirements, it reaches the collection drum 16 for rotation collection, and can also be introduced into a treatment tank containing sodium hydroxide solution for purification.

[0046] (3) Material processing

[0047] The prepared bacterial cellulose material is removed from the collecting drum 16, rinsed with distilled water for several times, and then immersed in a 0.5wt% NaOH solution and treated in a 70°C water bath for 30 minutes until the bacterial cellulose composite film becomes white and translucent. It is then washed 4 to 5 times with an aqueous solution containing 0.5mol / L acetic acid, and then repeatedly washed with pure water until neutral, to obtain a bacterial cellulose composite film product.

[0048] Example 2

[0049] (1) Bacterial culture

[0050] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0051] (2) Fermentation preparation of bacterial cellulose composite membrane

[0052] Using printed cotton cloth as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 1 The type of culture medium container 1, and then the skeleton material 13 is disturbed and cultured at a speed of 280m / h, and after dynamic culture at 30°C for 18h, the bacterial cellulose / cotton cloth printed composite film can be obtained;

[0053] (3) Material processing

[0054] The prepared bacterial cellulose material is removed from the collecting drum 16, rinsed with distilled water for several times, and then immersed in a 1wt% NaOH solution, treated in a water bath at 80°C for 100min, until the bacterial cellulose scaffold composite membrane is white and translucent, first washed with an aqueous solution containing 0.5mol / L acetic acid for 4 to 5 times, then repeatedly washed with pure water until neutral, and then refrigerated to obtain a bacterial cellulose scaffold composite membrane product.

[0055] Example 3

[0056] (1) Bacterial culture

[0057] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0058] (2) Fermentation preparation of bacterial cellulose composite membrane

[0059] Using medical gauze as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 1 In a culture medium container 1 of type, the skeleton material 13 is fermented at a speed of 104 m / h for 48 h to obtain a cellulose / gauze composite material;

[0060] (3) Material processing

[0061] The prepared bacterial cellulose material is removed from the collecting drum 16, rinsed with distilled water for several times, and then immersed in a 1wt% NaOH solution, treated in a water bath at 80°C for 100min, until the bacterial cellulose scaffold composite membrane is white and translucent, first washed with an aqueous solution containing 0.5mol / L acetic acid for 4 to 5 times, then repeatedly washed with pure water until neutral, and then refrigerated to obtain a bacterial cellulose scaffold composite membrane product.

[0062] Example 4

[0063] (1) Bacterial culture

[0064] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0065] (2) Fermentation preparation of bacterial cellulose composite membrane

[0066] Using nylon fiber as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 1 The type of culture medium container 1, and then the skeleton material 13 is disturbed and cultured at a speed of 100m / h, and after dynamic culture at 30°C for 50h, the bacterial cellulose / nylon composite membrane can be obtained;

[0067] (3) Material processing

[0068] The prepared bacterial cellulose material is removed from the collecting drum 16, rinsed with distilled water for several times, and then immersed in a 1wt% NaOH solution, treated in a water bath at 80°C for 100min, until the bacterial cellulose scaffold composite membrane is white and translucent, first washed with an aqueous solution containing 0.5mol / L acetic acid for 4 to 5 times, then repeatedly washed with pure water until neutral, and then refrigerated to obtain a bacterial cellulose scaffold composite membrane product.

[0069] Example 5

[0070] like Figure 4 As shown, the culture medium container 1 is a closed rectangular container, which is 200 meters long, 2 meters wide and 3 meters high. There are 1000 parallel rolling shafts 11 in the upper and lower rows of the container. The distance between the upper and lower axes of the rolling shafts 11 is 2.5 meters, the distance between the left and right axes of the rolling shafts 11 is 0.2 meters, and the diameter of the roller is 0.1 meters.

[0071] (1) Bacterial culture

[0072] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0073] (2) Fermentation preparation of bacterial cellulose composite membrane

[0074] Using nylon fiber as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 4 The type of culture medium container 1, and then the skeleton material 13 is disturbed and cultured at a speed of 100m / h, and after dynamic culture at 30°C for 50h, the bacterial cellulose / nylon composite membrane can be obtained;

[0075] During the fermentation process, the culture medium container 1 uses an air compressor to introduce filtered air, and the air flow rate is 1Nm3 / h.

[0076] Example 6

[0077] (1) Bacterial culture

[0078] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0079] (2) Fermentation preparation of bacterial cellulose composite membrane

[0080] Using cellulose fiber as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 1 The type culture medium container 1, and then the skeleton material 13 is disturbed and cultured at a speed of 100m / h, and after dynamic culture at 30°C for 50h, a composite membrane can be obtained;

[0081] (3) Material processing

[0082] The prepared bacterial cellulose material is removed from the collecting drum 16, rinsed with distilled water for several times, and then immersed in a 1wt% NaOH solution, treated in a water bath at 80°C for 100min, until the bacterial cellulose skeleton composite membrane is white and translucent, first washed with an aqueous solution containing 0.5mol / L acetic acid for 4 to 5 times, then repeatedly washed with pure water until neutral, and then refrigerated to obtain a bacterial cellulose scaffold composite membrane product.

[0083] Example 7

[0084] (1) Bacterial culture

[0085] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0086] (2) Fermentation preparation of bacterial cellulose composite membrane

[0087] Using medical gauze or wool or other protein fibers as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 1The skeleton material 13 is placed in a culture medium container 1 and then subjected to disturbance culture at a speed of 100 m / h. During this period, nanosilver material is regularly added to the culture medium. The addition of nanosilver material can be batch feeding or continuous addition. After dynamic culture at 30°C for 50 hours, a silver-loaded bacterial cellulose / gauze composite membrane or a silver-loaded bacterial cellulose / wool composite membrane can be obtained.

[0088] Example 8

[0089] (1) Bacterial culture

[0090] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0091] (2) Fermentation preparation of bacterial cellulose composite membrane

[0092] Using medical gauze or wool or other protein fibers as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 1 The type culture medium container 1 is then subjected to disturbance culture by the skeleton material 13 at a speed of 100 m / h. During this period, starch, gelatin or micro-nano SiO2 materials are regularly added to the culture medium. After dynamic culture at 30°C for 50 hours, bacterial cellulose / gauze / starch composite film, bacterial cellulose / wool / starch composite film, bacterial cellulose / gauze / gelatin composite film, bacterial cellulose / wool / gelatin composite film, bacterial cellulose / gauze / SiO2 composite film, or bacterial cellulose / wool / SiO2 composite film can be obtained. The addition of nanosilver material can be batch feeding addition or continuous addition.

[0093] Example 9

[0094] like Figure 5-7 The culture medium container 1 shown is an open rectangular container, 200 meters long, 2 meters wide and 3 meters high. There are 15 parallel rollers in the left and right rows of the container. The distance between the upper and lower axes of the rollers is 0.2 meters, the distance between the left and right axes of the rollers is 195 meters, and the roller diameter is 0.1 meters.

[0095] (1) Bacterial culture

[0096] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0097] (2) Fermentation preparation of bacterial cellulose composite membrane

[0098] Using medical gauze as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 5 The type culture medium container 1 is then subjected to disturbance culture by the skeleton material 13 at a speed of 100 m / h. After dynamic culture at 30° C. for 59 h, the bacterial cellulose / cotton printed composite film can be obtained.

[0099] Example 10

[0100] like Figure 8 The culture medium container 1 shown is a closed rectangular container, which is 200 meters long, 2 meters wide and 3 meters high. There are 15 parallel rollers in the left and right rows of the container. The distance between the upper and lower axes of the rollers is 0.2 meters, the distance between the left and right axes of the rollers is 195 meters, and the roller diameter is 0.1 meters.

[0101] (1) Bacterial culture

[0102] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0103] (2) Fermentation preparation of bacterial cellulose composite membrane

[0104] Using nylon fiber as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Figure 8 The type culture medium container 1 is then subjected to disturbance culture by the skeleton material 13 at a speed of 100 m / h. After dynamic culture at 30° C. for 59 h, the bacterial cellulose / nylon composite membrane can be obtained.

[0105] During the fermentation process, the culture medium container 1 uses an air compressor to introduce filtered air, and the air flow rate is 1Nm3 / h.

[0106] Embodiment 11

[0107] like Fig. 9 The culture medium container 1 shown is a closed rectangular container, which is 200 meters long, 2 meters wide and 3 meters high. There are 15 parallel rollers in the left and right rows of the container. The distance between the upper and lower axes of the rollers is 0.2 meters, the distance between the left and right axes of the rollers is 195 meters, and the roller diameter is 0.1 meters.

[0108] (1) Bacterial culture

[0109] The xyloglucanacetobacter seed solution was inoculated into 300L liquid culture medium (100g maltose, 3g peptone, 5g yeast extract, pH 4.5, sterilized at 115°C for 30min per 1L water; or 160g fructose, 5g yeast extract, 5g tryptone, 1.15g citric acid, 2.7g Na2HPO4, 1L water, pH 5.0, sterilized at 115°C for 30min) at a 10% inoculation rate, and the bioreactor was controlled at a constant temperature of 30°C;

[0110] (2) Fermentation preparation of bacterial cellulose composite membrane

[0111] Using nylon fiber mesh / polyester fiber mesh as the skeleton material 13, the liquid culture medium containing the production strain prepared in step (1) is transferred to Fig. 9 The skeleton material 13 is placed in a culture medium container 1, and then the skeleton material 13 is disturbed and cultured at a speed of 100 m / h. In the initial production, since the skeleton material has not yet attached to BC, it does not need to be collected directly. Instead, the skeleton material passes through the roller on the left and goes back to the starting roller on the right from the top to form a closed loop for production. When BC is successfully attached to the skeleton material, the roller on the left can be switched to the collection roller for harvesting. After 100 hours of cyclic dynamic culture in a culture medium at 30°C, the bacterial cellulose / nylon composite membrane can be obtained.

[0112] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A device for producing a bacterial cellulose composite material, comprising a culture medium container (1), a rolling shaft (11), a skeleton material (13), a feeding drum (15), a collecting drum (16), a guide roller (17) and a pressing roller (10), characterized in that: The rolling shafts (11) are arranged in two parallel rows inside the culture medium container (1), the rolling shafts (11) in each row are arranged in parallel and spaced apart, and the rolling shafts (11) in the two rows are parallel and offset to each other, the skeleton material (13) is fed by a feeding drum (15) and guided by a guide roller (17) into the culture medium container (1), staggered along the two rows of rolling shafts (11), and rolled by the rolling shafts (11) in an S-shaped track to be transported in the culture medium container (1), pulled out of the culture medium container (1), passed through a pressing roller (10), and guided into a collecting drum (16) for winding and collection; After passing through the pressing roller (10), the skeleton material (13) can be guided back to the starting rolling shaft (11) by the roller, forming a closed loop for cyclic production, until bacterial cellulose is successfully attached to the skeleton material (13), and then guided into the collecting drum (16) for winding and collection; The side wall of the culture medium container (1) is provided with a vent (3) and a dissolved oxygen electrode socket (5), and a dissolved oxygen electrode is installed in the dissolved oxygen electrode socket (5); The rolling shafts (11) are arranged in two rows horizontally and parallel to each other in the culture medium container (1), the skeleton material (13) is staggered in the vertical direction around the rolling shafts (11) staggered in the vertical direction, and the liquid level of the culture medium container (1) is controlled between the upper and lower rows of rolling shafts (11); or The rolling shafts (11) are arranged in two vertically parallel rows inside the culture medium container (1), and the skeleton material (13) is staggered and strung around the rolling shafts (11) staggered in the horizontal direction. The rolling shafts (11) in the upper part of the two rows of rolling shafts (11) are higher than the liquid level of the feed liquid.

2. The device for producing bacterial cellulose composite material according to claim 1, characterized in that: The culture medium container (1) is provided with a heating plate (2) inside and a liquid discharge port (14) at the bottom; the side wall of the culture medium container (1) is provided with a pH meter socket (4), a thermometer socket (6), an acid and alkali solution addition port (7), a nutrient supplement port (8), a liquid height probe shaft (9) and a feed port (12); a pH electrode is installed in the pH meter socket (4), a thermometer probe is installed in the thermometer socket (6), and a liquid level detection probe is installed inside the culture medium container (1).

3. The device for producing bacterial cellulose composite material according to claim 1, characterized in that: The feeding drum (15) and the collecting drum (16) are respectively arranged on two sides of the culture medium container (1) opposite to each other, and the guide roller (17) and the pressure roller (10) are correspondingly arranged on two opposite sides of the culture medium container (1) and located at the top edge of the culture medium container (1).

4. The device for producing bacterial cellulose composite material according to claim 1, characterized in that: The skeleton material (13) is a membrane material or a woven fabric material with a mesh, and the pore size of the mesh is 0.05 mm-10 mm.

5. The device for producing bacterial cellulose composite material according to claim 1, characterized in that: The culture medium container (1) is a closed container or an open-top container.

6. The device for producing bacterial cellulose composite material according to claim 1, characterized in that: The linear speed of the skeleton material (13) is 10 to 280 m / h.

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