Stem cell extraction process

Abstract

The invention relates to the technical field of stem cell extraction, in particular to a stem cell extraction process, solves the problems that when stem cells in a placenta are extracted in the prior art, the temperature in the placenta treatment process is inconvenient to keep stable, and the placenta is inconvenient to fully crush and clean, and is used for extracting the placenta stem cells. The stem cell extraction process is realized through stem cell extraction equipment, the stem cell extraction equipment comprises a circulating cooling mechanism, a coarse crushing mechanism, a fine crushing mechanism and a digestion separation mechanism, the coarse crushing mechanism is mounted on the inner side of the upper end of the circulating cooling mechanism, the fine crushing mechanism is mounted at the bottom end of the coarse crushing mechanism, and the digestion separation mechanism is mounted at the bottom end of the fine crushing mechanism. A digestion and separation mechanism is mounted on one side of the fine crushing mechanism, and the circulating cooling mechanism comprises a supporting base. Through continuous cleaning and gas injection in the stem cell extraction process, the temperature stability of the stem cells can be effectively kept, and the placenta cleaning and crushing effects can be conveniently improved.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell extraction, in particular to a stem cell extraction process. Background Art

[0002] Stem cells refer to primitive cells with the potential for self-renewal and multidirectional differentiation. They are the origin cells of the body and can differentiate into one or more cells that constitute human tissues or organs under specific conditions. Self-renewal means that stem cells can maintain the size of the stem cell population through cell division; multidirectional differentiation means that stem cells have plasticity and can differentiate into different tissue cells under different environments to form various complex tissues and organs of the body. Adipose stem cells are a type of stem cell with multidirectional differentiation potential that has been isolated from adipose tissue in recent years. Under specific induction conditions, they can differentiate into osteoblasts, chondrocytes, adipocytes, etc.

[0003] When extracting stem cells from the placenta, it is not convenient to maintain a stable temperature during the placenta processing process, and it is not convenient to fully crush and clean the placenta; therefore, it does not meet the existing needs. We have proposed a stem cell extraction process. Summary of the invention

[0004] The object of the present invention is to provide a stem cell extraction process to solve the problems raised in the above background technology that it is inconvenient to maintain a stable temperature during the placenta processing process and it is inconvenient to fully crush and clean the placenta when extracting stem cells from the placenta.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A stem cell extraction process for extracting placental stem cells, the stem cell extraction process is implemented by a stem cell extraction device, the stem cell extraction device includes a circulating cooling mechanism, a coarse grinding mechanism, a fine grinding mechanism and a digestion separation mechanism, the inner side of the upper end of the circulating cooling mechanism is equipped with a coarse grinding mechanism, the bottom end of the coarse grinding mechanism is equipped with a fine grinding mechanism, and one side of the fine grinding mechanism is equipped with a digestion separation mechanism, the circulating cooling mechanism includes a supporting base, the upper end surface of the supporting base is fixedly equipped with a protective box; the fine grinding mechanism includes two connecting angle plates , the two connecting angle plates are fixedly connected to the protection box, an outer conical material guide seat is fixedly installed between the two connecting angle plates, a third transmission motor is fixedly installed on the upper end surface of the outer conical material guide seat, an inner conical material guide seat is installed on the inner side of the outer conical material guide seat, a material shifting frame is rotatably connected between the outer conical material guide seat and the inner conical material guide seat, a driven crushing disk is fixedly installed on the inner side of the upper end of the inner conical material guide seat, an active crushing disk is rotatably connected above the driven crushing disk, and a transmission gear ring is fixedly installed on the side of the upper end surface of the active crushing disk;

[0007] The stem cell extraction process comprises the following steps:

[0008] S1: placing the placenta for stem cell extraction into the inner side of the coarse pulverizing mechanism, so that the placenta can be coarsely pulverized and transported under secondary pressure through the coarse pulverizing mechanism, thereby reducing the difficulty of placenta transportation and cleaning, and then the placenta enters the inner side of the fine pulverizing mechanism for fine pulverization after coarse pulverization;

[0009] S2: simultaneously inputting the cleaning liquid into the inner side of the coarse crushing mechanism, so that the placenta can be cleaned synchronously with the secondary crushing of the placenta by the cleaning liquid;

[0010] S3: The pulverized placenta can be separated from the cleaning solution through the digestion and separation mechanism, thereby reducing the content of the cleaning solution in the granular placenta;

[0011] S4: Collagenase and dextran are added at once during the subsequent digestion and centrifugation process, thereby facilitating the production of a suspension consisting of single cells, thereby facilitating the extraction of stem cells;

[0012] S5: During the placenta processing, continuous ventilation and circulating cooling are performed through the circulating cooling mechanism, thereby maintaining the temperature of the placenta stable before and after crushing.

[0013] Preferably, the circulating cooling mechanism also includes a transparent observation plate fixedly connected to one side of the protection box, a ventilation box is fixedly installed on the inner side of the bottom end of the protection box, a ventilation plate is fixedly installed at one end of the ventilation box, return flow sleeve plates are installed on the front and rear end surfaces of the ventilation box, and an air inlet duct is installed on the upper end surface of the ventilation box.

[0014] Preferably, the coarse grinding mechanism includes a conical feed seat, which is fixedly connected to the upper end of the protective box, and the inner side of the upper end of the conical feed seat is rotatably connected to two movable sealing plates, and a first transmission motor is fixedly installed between the two movable sealing plates, and a cleaning liquid injection pipe is installed on one side of the first transmission motor, and the inner side of the bottom end of the conical feed seat is rotatably connected to a first spiral conveying blade, and a first transmission shaft is fixedly installed on the inner side of the first spiral conveying blade, a central crushing roller is installed below the first spiral conveying blade, and a second spiral conveying blade is installed below the central crushing roller, and a sealing cover is fixedly installed at the bottom end of the conical feed seat, and a coarse grinding shell is installed on the lower end surface of the sealing cover, and second transmission motors are fixedly installed on both ends of the upper end surface of the sealing cover, and two auxiliary crushing rollers are rotatably connected to the inner side of the upper end of the coarse grinding shell, and a second transmission shaft is installed on the inner side of the auxiliary crushing roller.

[0015] Preferably, the digestion and separation mechanism includes a filtering and separation box, the upper end of which is fixedly connected to an outer conical material guide seat, a drain pipe is provided on the front end face of the filtering and separation box, a digestion box is fixedly installed at one end of the filtering and separation box, a centrifuge tank is installed on one side of the digestion box, the digestion box and the centrifuge tank are connected through a conveying elbow, a fourth transmission motor is fixedly installed in the middle of the upper end face of the centrifuge tank, and a discharge pipe is fixedly provided at the bottom end of the centrifuge tank.

[0016] Preferably, the outer conical material guide seat is fixedly connected to the protective box by two connecting angle plates, the outer conical material guide seat is fixedly connected to the inner conical material guide seat, and three material scrapers are provided on the outer side of the material shifting frame, and the three material scrapers are arranged in a circle relative to the axis of the active crushing disk, and the bottom end of the first transmission shaft sequentially passes through the first spiral conveying blade, the center crushing roller, the second spiral conveying blade, the driven crushing disk and the inner conical material guide seat and is fixedly connected to the middle part of the material shifting frame.

[0017] Preferably, a bearing is provided between the active crushing disk and the upper end of the inner conical material guide seat, a gear is fixedly provided at the output end of the third transmission motor, the transmission gear ring is connected to the gear through tooth meshing, the output end of the third transmission motor is connected to the active crushing disk through the gear and the transmission gear ring, and a plurality of crushing teeth are provided on the side of the active crushing disk close to the driven crushing disk.

[0018] Preferably, a fine grinding chamber is provided between the active grinding disk and the driven grinding disk, the bottom end of the coarse grinding shell passes through the active grinding disk and is connected with the fine grinding chamber, the coarse grinding shell is rotatably connected with the active grinding disk, and the coarse grinding shell is fixedly connected with the conical feed seat through a sealing cover.

[0019] Preferably, the output end of the second transmission motor passes through the sealing cover and is fixedly connected to the auxiliary crushing roller through the second transmission shaft, the two auxiliary crushing rollers have opposite rotation directions to the central crushing roller, the first spiral conveying blade, the central crushing roller and the second spiral conveying blade are all fixedly connected to the first transmission shaft, the upper end of the first transmission shaft passes through the conical feed seat and is connected to the output end of the first transmission motor through a coupling, the conical feed seat is injected with cleaning liquid through a cleaning liquid injection pipe, and the cleaning liquid is phosphate buffer solution.

[0020] Preferably, a fan and a condenser are provided on the inner side of the ventilation box, the fan input end is through-connected with the air-permeable plate and two return sleeves, the fan is connected to the air inlet pipe through the condenser, the coarse grinding shell is through-connected with the two return sleeves, the upper end of the air inlet pipe is inserted into the inner side of the bottom end of the outer conical material guide seat, and a one-way valve is provided on the inner side of the upper end of the air inlet pipe.

[0021] Preferably, a filter screen is provided on the inner side of the filtration and separation box, the rear end of the drainage pipe is connected to the outer conical material guide seat through the filtration and separation box, the filtration and separation box is connected to the digestion box, the inner side of the digestion box is filled with collagenase, the interior of the centrifuge tank is filled with dextran, a centrifugal roller is provided on the inner side of the centrifuge tank, and the output end of the fourth transmission motor passes through the centrifuge tank and is fixedly connected to the centrifugal roller.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention can pressurize and convey the placenta to the inner side of the coarse crushing shell through the first spiral conveying blade, and the second transmission motor drives the auxiliary crushing roller to rotate, so that the central crushing roller and the two auxiliary crushing rollers can perform a coarse crushing operation on the placenta, and the third transmission motor drives the active crushing disk to rotate relative to the driven crushing disk through the gear and the transmission gear ring, so that the active crushing disk and the driven crushing disk can perform a fine crushing operation on the placenta, and the cleaning liquid can be diverted through the conical conveying seat and the coarse crushing shell to perform a continuous cleaning operation while coarsely and finely crushing the placenta;

[0024] 2. The present invention injects cold air while the placenta is in continuous contact with the cleaning liquid during the coarse and fine grinding process. The cold air cools the cleaning liquid to keep the temperature of the placenta and the grinding structure stable, thereby improving the cooling effect of the cold air. After the fine grinding, three circularly arranged material scrapers are provided on the outside of the material feeding frame to scrape and transport the placenta material. At the same time, the material scrapers and the cleaning liquid can effectively prevent the material from adhering to the outer conical material guide seat and the inner conical material guide seat. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 A front view of the present invention as a whole;

[0027] Figure 3 It is a schematic diagram of the installation structure of the fine grinding mechanism of the present invention;

[0028] Figure 4 It is a structural schematic diagram of the coarse crushing mechanism of the present invention;

[0029] Figure 5 It is a schematic cross-sectional structure diagram of the coarse pulverizing mechanism of the present invention;

[0030] Figure 6 It is a partial cross-sectional structural schematic diagram of the fine grinding mechanism of the present invention;

[0031] Figure 7 It is a schematic diagram of the cross-sectional structure of the coarse grinding shell of the present invention;

[0032] Figure 8 It is a schematic diagram of the cross-sectional structure of the material diverter rack of the present invention.

[0033] In the figure: 1. Circulation cooling mechanism; 101. Support base; 102. Protection box; 103. Transparent observation plate; 104. Ventilation box; 105. Reflux sleeve; 106. Air inlet pipe; 107. Ventilation plate; 2. Coarse grinding mechanism; 201. Conical feeding seat; 202. Cleaning liquid injection pipe; 203. Movable sealing plate; 204. First transmission motor; 205. Coarse grinding shell; 206. Second transmission motor; 207. Sealing cover; 208. First transmission shaft; 209. First spiral conveying blade; 210. Center grinding roller; 211. Two spiral conveying blades; 212, auxiliary crushing roller; 213, second transmission shaft; 3, fine crushing mechanism; 301, connecting angle plate; 302, outer conical material guide seat; 303, third transmission motor; 304, active crushing disk; 305, inner conical material guide seat; 306, driven crushing disk; 307, transmission gear ring; 308, material shifting rack; 4, digestion and separation mechanism; 401, digestion box; 402, centrifugal tank; 403, conveying elbow; 404, fourth transmission motor; 405, discharge pipe; 406, filter separation box; 407, liquid discharge pipe. DETAILED DESCRIPTION

[0034] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0035] The first transmission motor 204 (model GV50-3.7KW-60-S), the second transmission motor 206 (model KOM7080), the third transmission motor 303 (model MDSKSRS080) and the fourth transmission motor 404 (model YS7134) mentioned in the present invention can all be purchased from the market or obtained through private customization.

[0036] See also Figures 1 to 8 The present invention provides an embodiment: a stem cell extraction process for extracting placental stem cells, the stem cell extraction process is implemented by a stem cell extraction device, the stem cell extraction device is used to extract placental stem cells, the stem cell extraction process is implemented by the stem cell extraction device, the stem cell extraction device includes a circulating cooling mechanism 1, a coarse crushing mechanism 2, a fine crushing mechanism 3 and a digestion separation mechanism 4, the coarse crushing mechanism 2 is installed on the inner side of the upper end of the circulating cooling mechanism 1, the fine crushing mechanism 3 is installed at the bottom end of the coarse crushing mechanism 2, and the digestion separation mechanism 4 is installed on one side of the fine crushing mechanism 3, and the stem cell extraction process includes the following steps:

[0037] S1: placing the placenta for stem cell extraction into the inner side of the coarse pulverizing mechanism 2, so that the placenta can be coarsely pulverized and conveyed under secondary pressure through the coarse pulverizing mechanism 2, thereby reducing the difficulty of conveying and cleaning the placenta, and then the placenta enters the inner side of the fine pulverizing mechanism 3 for fine pulverization after coarse pulverization;

[0038] S2: simultaneously inputting the cleaning liquid into the inner side of the coarse crushing mechanism 2, so that the placenta can be cleaned synchronously with the secondary crushing of the placenta by the cleaning liquid;

[0039] S3: The pulverized placenta and the cleaning solution can be separated by the digestion and separation mechanism 4, thereby reducing the content of the cleaning solution in the granular placenta;

[0040] S4: Collagenase and dextran are added at once during the subsequent digestion and centrifugation process, thereby facilitating the production of a suspension consisting of single cells, thereby facilitating the extraction of stem cells;

[0041] S5: During the placenta processing, continuous ventilation and circulating cooling are performed through the circulating cooling mechanism 1, thereby being able to maintain a stable temperature of the placenta before and after pulverization.

[0042] See also Figures 1 to 5 The circulating cooling mechanism 1 includes a supporting base 101, a protective box 102 is fixedly installed on the upper end surface of the supporting base 101, a transparent observation plate 103 is fixedly installed on one side of the protective box 102, a ventilation box 104 is fixedly installed on the inner side of the bottom end of the protection box 102, a ventilation plate 107 is fixedly installed on one end of the ventilation box 104, a return flow sleeve 105 is installed on the front and rear end surfaces of the ventilation box 104, an air inlet pipe 106 is installed on the upper end surface of the ventilation box 104, a fan and a condenser are arranged on the inner side of the ventilation box 104, the fan input end is connected with the air permeable plate 107 and the two return flow sleeves 105, the fan is connected with the air inlet pipe 106 through the condenser, a one-way valve is arranged on the inner side of the upper end of the air inlet pipe 106, the coarse grinding mechanism 2 and the fine grinding mechanism 3 can be circulated and ventilated through the air inlet pipe 106 and the two return flow sleeves 105, so as to keep the temperature of the placenta stable during the secondary grinding.

[0043] See also Figures 2 to 7 The coarse crushing mechanism 2 includes a conical conveying seat 201, which is fixedly connected to the upper end of the protective box 102. Two movable sealing plates 203 are rotatably connected to the inner side of the upper end of the conical conveying seat 201. A first transmission motor 204 is fixedly installed between the two movable sealing plates 203. A cleaning liquid injection pipe 202 is installed on one side of the first transmission motor 204. Cleaning liquid is injected into the conical conveying seat 201 through the cleaning liquid injection pipe 202. The cleaning liquid is a phosphate buffer solution. By injecting the cleaning liquid when the placenta is coarsely crushed, it is convenient to fully clean the placenta.

[0044] A first spiral conveying blade 209 is rotatably connected to the inner side of the bottom end of the conical conveying seat 201, a first transmission shaft 208 is fixedly installed on the inner side of the first spiral conveying blade 209, the upper end of the first transmission shaft 208 passes through the conical conveying seat 201 and is connected to the output end of the first transmission motor 204 through a coupling, a central crushing roller 210 is installed below the first spiral conveying blade 209, a second spiral conveying blade 211 is installed below the central crushing roller 210, the first spiral conveying blade 209, the central crushing roller 210 and the second spiral conveying blade 211 are all fixedly connected to the first transmission shaft 208, and the placenta can be subjected to secondary pressurized conveyance through the first spiral conveying blade 209 and the second spiral conveying blade 211;

[0045] A sealing cover 207 is fixedly installed at the bottom end of the conical feed seat 201, and a coarse grinding shell 205 is installed on the lower end surface of the sealing cover 207. The coarse grinding shell 205 is connected with the two reflux sleeves 105, and the coarse grinding shell 205 is fixedly connected to the conical feed seat 201 through the sealing cover 207. The second transmission motor 206 is fixedly installed at both ends of the upper end surface of the sealing cover 207. Two auxiliary grinding rollers 212 are rotatably connected to the inner side of the upper end of the coarse grinding shell 205. The two auxiliary grinding rollers 212 have opposite rotation directions to the central grinding roller 210. A second transmission shaft 213 is installed on the inner side of the auxiliary grinding roller 212. The output end of the second transmission motor 206 passes through the sealing cover 207 and is fixedly connected to the auxiliary grinding roller 212 through the second transmission shaft 213, so that the second transmission motor 206 drives the auxiliary grinding roller 212 to rotate, and then the central grinding roller 210 and the two auxiliary grinding rollers 212 can perform coarse grinding operations on the placenta.

[0046] See also Figures 3 to 8The fine grinding mechanism 3 includes two connecting angle plates 301, the two connecting angle plates 301 are fixedly connected to the protection box 102, an outer conical material guide seat 302 is fixedly installed between the two connecting angle plates 301, the outer conical material guide seat 302 is fixedly connected to the protection box 102 through the two connecting angle plates 301, the upper end of the air inlet pipe 106 is plugged into the inner side of the bottom end of the outer conical material guide seat 302, a third transmission motor 303 is fixedly installed on the upper end surface of the outer conical material guide seat 302, an inner conical material guide seat 305 is installed on the inner side of the outer conical material guide seat 302, and a material shifting frame 30 is rotatably connected between the outer conical material guide seat 302 and the inner conical material guide seat 305. 8. The outer conical material guide seat 302 is fixedly connected to the inner conical material guide seat 305. Three material-dispensing scrapers are arranged on the outer side of the material-dispensing frame 308. The three material-dispensing scrapers are arranged in a circle relative to the axis of the active crushing disk 304. The bottom end of the first transmission shaft 208 passes through the first spiral conveying blade 209, the central crushing roller 210, the second spiral conveying blade 211, the driven crushing disk 306 and the inner conical material guide seat 305 in sequence and is fixedly connected to the middle of the material-dispensing frame 308. The placenta material can be scraped and conveyed by the material-dispensing scraper. At the same time, the material-dispensing scraper and the cleaning liquid can effectively prevent the material from adhering to the outer conical material guide seat 302 and the inner conical material guide seat 305.

[0047] A driven crushing disc 306 is fixedly installed on the inner side of the upper end of the inner conical guide seat 305, and an active crushing disc 304 is rotatably connected to the upper end of the driven crushing disc 306. A bearing is provided between the active crushing disc 304 and the upper end of the inner conical guide seat 305. A plurality of crushing teeth are provided on the side close to the active crushing disc 304 and the driven crushing disc 306. A fine crushing cavity is provided between the active crushing disc 304 and the driven crushing disc 306. The bottom end of the coarse crushing shell 205 penetrates the active crushing disc 304 and is connected to the fine crushing cavity. The coarse crushing shell 205 rotates with the active crushing disc 304. The active pulverizing disk 304 is dynamically connected, and a transmission ring gear 307 is fixedly installed on the side of the upper end surface of the active pulverizing disk 304. A gear is fixedly provided on the output end of the third transmission motor 303. The transmission ring gear 307 is connected to the gear through tooth meshing. The output end of the third transmission motor 303 is connected to the active pulverizing disk 304 through the gear and the transmission ring gear 307, so that the third transmission motor 303 drives the active pulverizing disk 304 to rotate relative to the driven pulverizing disk 306 through the gear and the transmission ring gear 307, and then the active pulverizing disk 304 and the driven pulverizing disk 306 can perform a fine pulverization operation on the placenta.

[0048] See also Figures 1 to 3The digestion and separation mechanism 4 includes a filtering and separation box 406, a filtering net is provided on the inner side of the filtering and separation box 406, the upper end of the filtering and separation box 406 is fixedly connected to the outer conical material guide seat 302, a drainage pipe 407 is provided on the front end face of the filtering and separation box 406, the rear end of the drainage pipe 407 is connected to the outer conical material guide seat 302 through the filtering and separation box 406, the filtering and separation box 406 is connected to the digestion box 401, one end of the filtering and separation box 406 is fixedly installed with the digestion box 401, the inner side of the digestion box 401 is filled with collagenase, and the placenta material can be digested through the digestion box 401;

[0049] A centrifuge tank 402 is installed on one side of the digestion box 401, and the digestion box 401 and the centrifuge tank 402 are connected through a conveying elbow 403. A fourth transmission motor 404 is fixedly installed in the middle of the upper end surface of the centrifuge tank 402. The interior of the centrifuge tank 402 is filled with dextran. A centrifuge roller is provided on the inner side of the centrifuge tank 402. The output end of the fourth transmission motor 404 passes through the centrifuge tank 402 and is fixedly connected to the centrifuge roller. A discharge pipe 405 is fixedly provided at the bottom end of the centrifuge tank 402, so that the dextran inside the centrifuge tank 402 can further process the placenta and obtain a suspension containing single cells, which is convenient for extracting stem cells.

[0050] In summary, when the stem cells in the placenta are extracted, the placenta is placed into the inner side of the conical feed seat 201 through the two movable sealing plates 203, the power is turned on, and the first transmission motor 204 is started, so that the first transmission motor 204 synchronously drives the first spiral conveying blade 209, the central crushing roller 210, the second spiral conveying blade 211 and the material shifting frame 308 to rotate through the first transmission shaft 208 under the support of the conical feed seat 201, and then the first spiral conveying blade 209 can pressurize and convey the placenta to the inner side of the coarse crushing shell 205, and the central crushing roller 210 and the two auxiliary crushing rollers 212 have opposite rotation directions;

[0051] The second transmission motor 206 is started, so that the second transmission motor 206 drives the auxiliary crushing roller 212 to rotate under the support of the sealing cover 207, so that the central crushing roller 210 and the two auxiliary crushing rollers 212 can perform a coarse crushing operation on the placenta, and then the second spiral conveying blade 211 pressurizes and conveys it to the fine crushing chamber provided between the active crushing disk 304 and the driven crushing disk 306, and the third transmission motor 303 is started, so that the third transmission motor 303 drives the active crushing disk 304 to rotate relative to the driven crushing disk 306 through the gear and the transmission gear ring 307 under the support of the outer conical material guide seat 302, so that the active crushing disk 304 and the driven crushing disk 306 can perform a fine crushing operation on the placenta;

[0052] During the placenta crushing process, the cleaning liquid is injected into the inner side of the conical material conveying seat 201 through the cleaning liquid injection pipe 202, so that the cleaning liquid can be diverted through the conical material conveying seat 201 and the coarse crushing shell 205 to perform a continuous cleaning operation while coarsely crushing and finely crushing the placenta. After finely crushing, three circumferentially arranged material scrapers are provided on the outer side of the material diverting frame 308 to scrape and convey the placenta material. At the same time, the material scrapers and the cleaning liquid can effectively prevent the material from adhering between the outer conical material guide seat 302 and the inner conical material guide seat 305.

[0053] When the outer conical material guide seat 302 conveys the material to the inner side of the filtering and separating box 406, the filter net provided on the inner side of the filtering and separating box 406 can filter and separate the cleaning liquid and the material, and discharge the cleaning liquid through the discharge pipe 407, and then the material is injected into the digestion box 401 filled with collagenase, and then the placenta material can be digested by the digestion box 401, and transported to the inner side of the centrifuge tank 402 through the conveying bend 403, so that the dextran inside the centrifuge tank 402 can further process the placenta and obtain a suspension containing single cells, which is convenient for the extraction of stem cells.

[0054] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A stem cell extraction process for extracting placental stem cells, characterized in that: The stem cell extraction process is implemented by a stem cell extraction device, which comprises a circulating cooling mechanism (1), a coarse grinding mechanism (2), a fine grinding mechanism (3) and a digestion and separation mechanism (4), wherein the coarse grinding mechanism (2) is installed on the inner side of the upper end of the circulating cooling mechanism (1), the fine grinding mechanism (3) is installed on the bottom end of the coarse grinding mechanism (2), and the digestion and separation mechanism (4) is installed on one side of the fine grinding mechanism (3), the circulating cooling mechanism (1) comprises a supporting base (101), and a protective box (102) is fixedly installed on the upper end surface of the supporting base (101); the fine grinding mechanism (3) comprises two connecting angle plates (301), and the two connecting angle plates (301) are fixed to the protective box (102). An outer conical material guide seat (302) is fixedly installed between the two connecting angle plates (301), a third transmission motor (303) is fixedly installed on the upper end surface of the outer conical material guide seat (302), an inner conical material guide seat (305) is installed on the inner side of the outer conical material guide seat (302), a material shifting frame (308) is rotatably connected between the outer conical material guide seat (302) and the inner conical material guide seat (305), a driven crushing disc (306) is fixedly installed on the inner side of the upper end of the inner conical material guide seat (305), an active crushing disc (304) is rotatably connected above the driven crushing disc (306), and a transmission gear ring (307) is fixedly installed on the side of the upper end surface of the active crushing disc (304); The stem cell extraction process The following steps are involved: S1: placing the placenta for stem cell extraction into the inner side of the coarse pulverizing mechanism (2), so that the placenta can be coarsely pulverized and transported under secondary pressure through the coarse pulverizing mechanism (2), thereby reducing the difficulty of transporting and cleaning the placenta, and then the placenta enters the inner side of the fine pulverizing mechanism (3) for fine pulverization after the coarse pulverization; S2: simultaneously inputting a cleaning liquid into the inner side of the coarse crushing mechanism (2), so that the placenta can be cleaned synchronously with the secondary crushing of the placenta by the cleaning liquid; S3: The pulverized placenta and the cleaning solution can be separated by the digestion and separation mechanism (4), thereby reducing the content of the cleaning solution in the granular placenta; S4: Collagenase and dextran are added at once during the subsequent digestion and centrifugation process, thereby facilitating the production of a suspension consisting of single cells, thereby facilitating the extraction of stem cells; S5: During the placenta processing, continuous ventilation and circulating cooling are performed through the circulating cooling mechanism (1), thereby being able to maintain a stable temperature of the placenta before and after pulverization.

2. The stem cell extraction process according to claim 1, characterized in that: The circulating cooling mechanism (1) also includes a transparent observation plate (103) fixedly connected to one side of the protection box (102); a ventilation box (104) is fixedly installed on the inner side of the bottom end of the protection box (102); a ventilation plate (107) is fixedly installed on one end of the ventilation box (104); a return sleeve plate (105) is installed on both the front and rear end surfaces of the ventilation box (104); and an air inlet pipe (106) is installed on the upper end surface of the ventilation box (104).

3. The stem cell extraction process according to claim 2, characterized in that: The coarse crushing mechanism (2) comprises a conical material conveying seat (201), the conical material conveying seat (201) is fixedly connected to the upper end of the protection box (102), the inner side of the upper end of the conical material conveying seat (201) is rotatably connected to two movable sealing plates (203), a first transmission motor (204) is fixedly installed between the two movable sealing plates (203), a cleaning liquid injection pipe (202) is installed on one side of the first transmission motor (204), the inner side of the bottom end of the conical material conveying seat (201) is rotatably connected to a first spiral conveying blade (209), and a first transmission shaft (208) is fixedly installed on the inner side of the first spiral conveying blade (209). A central crushing roller (210) is installed below the first spiral conveying blade (209), a second spiral conveying blade (211) is installed below the central crushing roller (210), a sealing cover (207) is fixedly installed at the bottom end of the conical feeding seat (201), a coarse crushing shell (205) is installed on the lower end surface of the sealing cover (207), and a second transmission motor (206) is fixedly installed at both ends of the upper end surface of the sealing cover (207), and two auxiliary crushing rollers (212) are rotatably connected to the inner side of the upper end of the coarse crushing shell (205), and a second transmission shaft (213) is installed on the inner side of the auxiliary crushing roller (212).

4. The stem cell extraction process according to claim 3, characterized in that: The digestion and separation mechanism (4) comprises a filtering and separation box (406), the upper end of which is fixedly connected to the outer conical material guide seat (302), the front end surface of the filtering and separation box (406) is provided with a discharge pipe (407), one end of the filtering and separation box (406) is fixedly mounted with a digestion box (401), one side of the digestion box (401) is mounted with a centrifuge tank (402), the digestion box (401) and the centrifuge tank (402) are connected through a conveying elbow (403), a fourth transmission motor (404) is fixedly mounted in the middle of the upper end surface of the centrifuge tank (402), and a discharge pipe (405) is fixedly mounted at the bottom end of the centrifuge tank (402).

5. The stem cell extraction process according to claim 4, characterized in that: The outer conical material guide seat (302) is fixedly connected to the protective box (102) via two connecting angle plates (301); the outer conical material guide seat (302) is fixedly connected to the inner conical material guide seat (305); three material-moving scrapers are arranged on the outer side of the material-moving frame (308); the three material-moving scrapers are arranged in a circle relative to the axis of the active crushing disk (304); the bottom end of the first transmission shaft (208) sequentially passes through the first spiral conveying blade (209), the central crushing roller (210), the second spiral conveying blade (211), the driven crushing disk (306) and the inner conical material guide seat (305) and is fixedly connected to the middle of the material-moving frame (308).

6. The stem cell extraction process according to claim 5, characterized in that: A bearing is provided between the active crushing disc (304) and the upper end of the inner conical material guide seat (305); a gear is fixedly provided at the output end of the third transmission motor (303); the transmission gear ring (307) is connected to the gear through tooth meshing; the output end of the third transmission motor (303) is connected to the active crushing disc (304) through the gear and the transmission gear ring (307); and a plurality of crushing teeth are provided on one side of the active crushing disc (304) close to the driven crushing disc (306).

7. The stem cell extraction process according to claim 6, characterized in that: A fine grinding chamber is provided between the active grinding disc (304) and the driven grinding disc (306); the bottom end of the coarse grinding shell (205) penetrates the active grinding disc (304) and is connected to the fine grinding chamber; the coarse grinding shell (205) is rotatably connected to the active grinding disc (304); and the coarse grinding shell (205) is fixedly connected to the conical feeding seat (201) via a sealing cover (207).

8. The stem cell extraction process according to claim 7, characterized in that: The output end of the second transmission motor (206) passes through the sealing cover (207) and is fixedly connected to the auxiliary crushing roller (212) via the second transmission shaft (213); the two auxiliary crushing rollers (212) have opposite rotation directions to the central crushing roller (210); the first spiral conveying blade (209), the central crushing roller (210) and the second spiral conveying blade (211) are all fixedly connected to the first transmission shaft (208); the upper end of the first transmission shaft (208) passes through the conical feed seat (201) and is connected to the output end of the first transmission motor (204) via a coupling; the conical feed seat (201) is injected with cleaning liquid via a cleaning liquid injection pipe (202); the cleaning liquid is a phosphate buffer solution.

9. The stem cell extraction process according to claim 8, characterized in that: A fan and a condenser are provided on the inner side of the ventilation box (104); the fan input end is connected to the air permeable plate (107) and the two return sleeves (105); the fan is connected to the air inlet pipe (106) through the condenser; the coarse grinding shell (205) is connected to the two return sleeves (105); the upper end of the air inlet pipe (106) is plugged into the inner side of the bottom end of the outer conical material guide seat (302); and a one-way valve is provided on the inner side of the upper end of the air inlet pipe (106).

10. The stem cell extraction process according to claim 9, characterized in that: A filter screen is provided on the inner side of the filtering and separating box (406); the rear end of the drain pipe (407) is connected to the outer conical material guide seat (302) through the filtering and separating box (406); the filtering and separating box (406) is connected to the digestion box (401); the inner side of the digestion box (401) is filled with collagenase; the interior of the centrifuge tank (402) is filled with dextran; a centrifugal roller is provided on the inner side of the centrifuge tank (402); and the output end of the fourth transmission motor (404) passes through the centrifuge tank (402) and is fixedly connected to the centrifugal roller.

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