Apirulina containing spirulina bioactive peptide and spirulina proteinase, preparation method and application thereof

Abstract

The invention discloses a spirulina with active spirulina peptide and spirulina prolease and preparing method and application, which is characterized by the following: adjusting human blood fat; adopting bacillus subtilis to ferment spirulina; making the active spirulina peptide at 15-40% when the water content is under 7%; possessing 1000-40000U / g spirulina prolease.

Description

technical field

[0001] The present invention relates to a kind of spirulina, especially a kind of spirulina containing spirulina active peptide and spirulina protease, and the spirulina active peptide and spirulina protease exist in molecular form; the present invention also relates to the spirulina Preparation method and application of the spirulina. Background technique

[0002] Spirulina contains a variety of amino acids, and also contains a variety of vitamins, carotene, unsaturated fatty acids and polysaccharides that are beneficial to the human body. It can provide the human body with a variety of nutrients, regulate the physiological functions of the human body, and enhance immunity. It is a kind of high nutrition. , Low-calorie natural food is undoubtedly the most ideal health care product for those who have an unreasonable nutritional structure. For diseases caused by lack of nutrients, it can supplement nutrition to achieve health care and cure diseases. At presen...

Examples

Embodiment 1

[0015] (1) Carry out strain culture first, get 1 branch of Bacillus subtilis (the Bacillus subtilis existing commercial sale, the Bacillus subtilis used in the present invention buys from Institute of Microbiology, Chinese Academy of Sciences) inoculates on nutrient gravy agar medium, nutrient gravy The agar medium is mainly composed of the following raw materials: 10 grams of peptone, 3 grams of beef extract, 5 grams of agar, 5 grams of sodium chloride, plus sterile water to 1000 ml, and adjust the pH to 7. Seven strains were cultured at 35°C for 64 hours according to the conventional strain culture method, and then the cultured strains were combined to obtain the strain culture solution;

[0016] (2) Get 3 milliliters of the above-mentioned strain culture solution and add 35 kilograms of spirulina powder to the spirulina pulp with a water ratio of 1: 1.3 and stir evenly, ferment for 18 hours under the condition of 37° C., stir once every 4 hours during this period, let The a...

Embodiment 2

[0022] (1) is identical with the (1) step of embodiment 1;

[0023] (2) Get 5 milliliters of the above-mentioned strain culture solution and add 35 kilograms of spirulina powder to the spirulina pulp with a water ratio of 1: 1.3 and stir evenly, seal and ferment for 18 hours under the condition of 37° C., stir once every 4 hours during this period, Allow the algae pulp to ferment evenly;

[0024] All the other steps are the same as in Example 1.

[0025] The invention prepared by the above method has been tested by the Center for Disease Control and Prevention of Guangxi Zhuang Autonomous Region. In addition to the ingredients contained in conventional spirulina, it also contains 38.5% of spirulina active peptide and 11000 U / g of spirulina protease.

technical field

[0001] The present invention relates to a kind of spirulina, especially a kind of spirulina containing spirulina active peptide and spirulina protease, and the spirulina active peptide and spirulina protease exist in molecular form; the present invention also relates to the spirulina Preparation method and application of the spirulina. Background technique

[0002] Spirulina contains a variety of amino acids, and also contains a variety of vitamins, carotene, unsaturated fatty acids and polysaccharides that are beneficial to the human body. It can provide the human body with a variety of nutrients, regulate the physiological functions of the human body, and enhance immunity. It is a kind of high nutrition. , Low-calorie natural food is undoubtedly the most ideal health care product for those who have an unreasonable nutritional structure. For diseases caused by lack of nutrients, it can supplement nutrition to achieve health care and cure diseases. At presen...

Examples

Embodiment 1

[0015] (1) Carry out strain culture first, get 1 branch of Bacillus subtilis (the Bacillus subtilis existing commercial sale, the Bacillus subtilis used in the present invention buys from Institute of Microbiology, Chinese Academy of Sciences) inoculates on nutrient gravy agar medium, nutrient gravy The agar medium is mainly composed of the following raw materials: 10 grams of peptone, 3 grams of beef extract, 5 grams of agar, 5 grams of sodium chloride, plus sterile water to 1000 ml, and adjust the pH to 7. Seven strains were cultured at 35°C for 64 hours according to the conventional strain culture method, and then the cultured strains were combined to obtain the strain culture solution;

[0016] (2) Get 3 milliliters of the above-mentioned strain culture solution and add 35 kilograms of spirulina powder to the spirulina pulp with a water ratio of 1: 1.3 and stir evenly, ferment for 18 hours under the condition of 37° C., stir once every 4 hours during this period, let The a...

Embodiment 2

[0022] (1) is identical with the (1) step of embodiment 1;

[0023] (2) Get 5 milliliters of the above-mentioned strain culture solution and add 35 kilograms of spirulina powder to the spirulina pulp with a water ratio of 1: 1.3 and stir evenly, seal and ferment for 18 hours under the condition of 37° C., stir once every 4 hours during this period, Allow the algae pulp to ferment evenly;

[0024] All the other steps are the same as in Example 1.

[0025] The invention prepared by the above method has been tested by the Center for Disease Control and Prevention of Guangxi Zhuang Autonomous Region. In addition to the ingredients contained in conventional spirulina, it also contains 38.5% of spirulina active peptide and 11000 U / g of spirulina protease.