Spirulina containing active peptide, protease and protein kinase, and its processing method and application

Abstract

The invention disclosed a kind of spirulina which contains spirulina active peptide, protease and kinas, the preparing method and application is also included in the invention. The spirulina contains all round nutrients and has the function of regulating the body blood fat, but the function is very mild and slow. Besides, there are no reports about the molecular spirulina active peptide, protease and kinas existing in spirulina. The invention has fermented the spirulina with Bacillus subtitles to get spirulina that contains spirulina active peptide, protease and kinas. The detecting results show that the spirulina contains 15-40% (W / W) active peptide, 100-10000U / g protease and 1000-80000FU / g kinas when the water content of the spirulina is lower than 7%. The clinical examination shows that the invention has the function of dissolving thrombus, preventing high viscosity blood disease, and hypertensive disease.

Description

technical field

[0001] The present invention relates to a kind of spirulina, especially a kind of spirulina containing spirulina active peptide, spirulina protease and protein kinase, and the spirulina active peptide, spirulina protease and protein kinase exist in molecular form; the present invention It also relates to the preparation method of the spirulina and the application of the spirulina. Background technique

[0002] Spirulina contains a variety of amino acids, and also contains a variety of vitamins, carotene, unsaturated fatty acids and polysaccharides that are beneficial to the human body. It can provide the human body with a variety of nutrients, regulate the physiological functions of the human body, and enhance immunity. It is a kind of high nutrition. , Low-calorie natural food is undoubtedly the most ideal health care product for those who have an unreasonable nutritional structure. For diseases caused by lack of nutrients, it can supplement nutrition to ach...

Examples

Embodiment 1

[0015] (1) Carry out strain culture first, get 1 branch of Bacillus subtilis (the Bacillus subtilis existing commercial sale, the Bacillus subtilis used in the present invention buys from Institute of Microbiology, Chinese Academy of Sciences) inoculates on nutrient gravy agar medium, nutrient gravy The agar medium is mainly composed of the following raw materials: 10 grams of peptone, 3 grams of beef extract, 5 grams of agar, 5 grams of sodium chloride, plus sterile water to 1000 ml, and adjust the pH to 7. Seven strains were cultured at 30-38°C for 24-48 hours according to the conventional strain culture method, and then the cultured strains were combined to obtain the strain culture solution;

[0016] (2) Take 15-30 ml of the above strain culture solution and add 35 kg of spirulina powder to the spirulina pulp with a water ratio of 1:1.3, stir evenly, and ferment for 18-24 hours at 37°C, during which every 4 hours Stir once to let the algae pulp ferment evenly;

[0017] (3...

Embodiment 2

[0022] (1) is identical with the (1) step of embodiment 1;

[0023] (2) Take 5 milliliters of the above strain culture solution and add 35 kilograms of spirulina powder to the spirulina slurry with a water ratio of 1:1.3 and stir evenly, and seal and ferment for 18-24 hours at 37°C, stirring every 4 hours during this period Once, let the algae pulp ferment evenly;

[0024] All the other steps are the same as in Example 1.

[0025] The present invention prepared by the above-mentioned method is tested by Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention. Except containing the ingredients contained in conventional spirulina, it also contains 16.6% of spirulina active peptide, 210U / g of spirulina protease, and 35000FU / g of spirulina protein kinase. g.

technical field

[0001] The present invention relates to a kind of spirulina, especially a kind of spirulina containing spirulina active peptide, spirulina protease and protein kinase, and the spirulina active peptide, spirulina protease and protein kinase exist in molecular form; the present invention It also relates to the preparation method of the spirulina and the application of the spirulina. Background technique

[0002] Spirulina contains a variety of amino acids, and also contains a variety of vitamins, carotene, unsaturated fatty acids and polysaccharides that are beneficial to the human body. It can provide the human body with a variety of nutrients, regulate the physiological functions of the human body, and enhance immunity. It is a kind of high nutrition. , Low-calorie natural food is undoubtedly the most ideal health care product for those who have an unreasonable nutritional structure. For diseases caused by lack of nutrients, it can supplement nutrition to ach...

Examples

Embodiment 1

[0015] (1) Carry out strain culture first, get 1 branch of Bacillus subtilis (the Bacillus subtilis existing commercial sale, the Bacillus subtilis used in the present invention buys from Institute of Microbiology, Chinese Academy of Sciences) inoculates on nutrient gravy agar medium, nutrient gravy The agar medium is mainly composed of the following raw materials: 10 grams of peptone, 3 grams of beef extract, 5 grams of agar, 5 grams of sodium chloride, plus sterile water to 1000 ml, and adjust the pH to 7. Seven strains were cultured at 30-38°C for 24-48 hours according to the conventional strain culture method, and then the cultured strains were combined to obtain the strain culture solution;

[0016] (2) Take 15-30 ml of the above strain culture solution and add 35 kg of spirulina powder to the spirulina pulp with a water ratio of 1:1.3, stir evenly, and ferment for 18-24 hours at 37°C, during which every 4 hours Stir once to let the algae pulp ferment evenly;

[0017] (3...

Embodiment 2

[0022] (1) is identical with the (1) step of embodiment 1;

[0023] (2) Take 5 milliliters of the above strain culture solution and add 35 kilograms of spirulina powder to the spirulina slurry with a water ratio of 1:1.3 and stir evenly, and seal and ferment for 18-24 hours at 37°C, stirring every 4 hours during this period Once, let the algae pulp ferment evenly;

[0024] All the other steps are the same as in Example 1.

[0025] The present invention prepared by the above-mentioned method is tested by Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention. Except containing the ingredients contained in conventional spirulina, it also contains 16.6% of spirulina active peptide, 210U / g of spirulina protease, and 35000FU / g of spirulina protein kinase. g.