Method for extracting active ingredients for reducing sugar blood from waste residues of liquorice

Abstract

The invention relates to a method for extracting active ingredients for reducing sugar blood from waste residues of liquorice. The method comprises the following steps of: taking dry liquorice residues; adding ethanol with the concentration of 65 percent to the liquorice residues with the material to liquid ratio of 1:12; extracting twice at the ultrasonic temperature of 75 DEG C and power of 40KHz, wherein the extraction time at every time is 1.5h; filtrating; merging filter liquors to obtain concentrated solution; recovering a solvent to obtain an extract; enabling the concentrate extract to pass through a sieve of 30-60 meshes and then carrying out polyamide column chromatography; sequentially carrying out gradient elution with water and 95% ethanol; reacting with hydrochloric acid-magnesium powder, detecting and merging to obtain general flavone; merging the products to obtain B; concentrating the B under a pressure reduction state; and freezing and drying in vacuum to obtain a product. The method extracting and separating active ingredients for reducing sugar blood from waste residues of the liquorice, has a simple preparation process and is suitable for industrial production.

Description

technical field

[0001] The invention relates to a method for extracting hypoglycemic active components from the waste residue of licorice. Background technique

[0002] Traditional Chinese medicine licorice is the dried root and rhizome of leguminous licorice (Glycyrrhiza uralensis Fisch.), licorice (Glycyrrhiza inflata Bat.) or licorice (Glycyrrhiza glabra L.). my country is the first country in the world to understand and study licorice, which is recorded in the "Shen Nong's Materia Medica" in the Eastern Han Dynasty. Its taste is sweet, its nature is flat, and it enters the heart, lung, spleen and stomach meridians. For the treatment of weak spleen and stomach, lack of gas, cough and asthma, carbuncle sore, abdominal spasm and pain and other symptoms and to ease the potency of drugs, detoxification. Scientific research experiments show that licorice is rich in glycyrrhizic acid, glycyrrhetic acid, flavonoids, saponins, alkaloids and amino acids, which have antiviral, an...

Examples

Embodiment 1

[0017] Experiment 1 Screening of extraction technology of licorice flavonoids in licorice waste residue

[0018] (1) Two technical routes of organic solvent thermal reflux method:

[0019] Take 100 g of dried licorice slag, add 2000 ml of 95% ethanol solution with a concentration of 95%, water-bath reflux temperature of 65 ° C, time of 60 min, filter, extract a total of 3 times, combine the filtrates, and concentrate under reduced pressure to obtain a concentrated solution;

[0020] a. Extract the concentrated solution 3 times with an equal volume of petroleum ether, discard the petroleum ether layer, add an equal volume of ethyl acetate and extract 3 times to obtain an ethyl acetate extract A 1 . will A 1 Wash with 5% sodium carbonate solution (adjusted to pH 8-9), then add 5% hydrochloric acid solution (adjusted to pH 5-6), let stand for precipitation, and the solution is A 2 , which precipitates A 3 ;

[0021] b. The concentrated liquid extract is subjected to 30-60 me...

technical field

[0001] The invention relates to a method for extracting hypoglycemic active components from the waste residue of licorice. Background technique

[0002] Traditional Chinese medicine licorice is the dried root and rhizome of leguminous licorice (Glycyrrhiza uralensis Fisch.), licorice (Glycyrrhiza inflata Bat.) or licorice (Glycyrrhiza glabra L.). my country is the first country in the world to understand and study licorice, which is recorded in the "Shen Nong's Materia Medica" in the Eastern Han Dynasty. Its taste is sweet, its nature is flat, and it enters the heart, lung, spleen and stomach meridians. For the treatment of weak spleen and stomach, lack of gas, cough and asthma, carbuncle sore, abdominal spasm and pain and other symptoms and to ease the potency of drugs, detoxification. Scientific research experiments show that licorice is rich in glycyrrhizic acid, glycyrrhetic acid, flavonoids, saponins, alkaloids and amino acids, which have antiviral, an...

Examples

Embodiment 1

[0017] Experiment 1 Screening of extraction technology of licorice flavonoids in licorice waste residue

[0018] (1) Two technical routes of organic solvent thermal reflux method:

[0019] Take 100 g of dried licorice slag, add 2000 ml of 95% ethanol solution with a concentration of 95%, water-bath reflux temperature of 65 ° C, time of 60 min, filter, extract a total of 3 times, combine the filtrates, and concentrate under reduced pressure to obtain a concentrated solution;

[0020] a. Extract the concentrated solution 3 times with an equal volume of petroleum ether, discard the petroleum ether layer, add an equal volume of ethyl acetate and extract 3 times to obtain an ethyl acetate extract A 1 . will A 1 Wash with 5% sodium carbonate solution (adjusted to pH 8-9), then add 5% hydrochloric acid solution (adjusted to pH 5-6), let stand for precipitation, and the solution is A 2 , which precipitates A 3 ;

[0021] b. The concentrated liquid extract is subjected to 30-60 me...

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