Microporous plate greenhouse culture device for HPV (human papillomavirus) detection

Abstract

The utility model discloses a microwell plate greenhouse culture device for HPV (human papillomavirus) detection, which comprises a box body, a box door is arranged on the front side of the box body, an air duct frame is arranged in an inner cavity of the box body, and the air duct frame comprises two oppositely distributed side air plates and an upper air plate positioned between the top ends of the two side air plates. An opening is formed in the middle of the surface of the upper air plate, an air guide seat is arranged at the opening, and a fan is arranged at the position, located above the air guide seat, of the inner wall of the top end of the box body. Under the action of the air guide seat, air flow passes through the upper air plate to flow towards the air channel formed by the side air plates on the two sides and the inner wall of the box body, enters the bottom between the two side air plates through the U-shaped opening in the bottom, and flows upwards through the ventilation opening in the culture plate; and finally, the air flows towards the side air plates on the left side and the right side through the air guide seat, so that the air in the inner cavity of the box body circularly flows, and the temperature in the box body is uniform.

Description

Technical Field

[0001] The utility model belongs to the technical field of HPV in vitro induction detection, and particularly relates to a microplate greenhouse culture device for HPV detection. Background Art

[0002] Clinical targeted T cell immunotherapy technology is a rapid and effective detection method that has just been invented and developed in the medical community in recent years. It is used for the detection and treatment of advanced cervical cancer. When conducting targeted T cell immunotherapy, a certain number of peripheral blood mononuclear cells are first obtained from the patient's vein, and then the test object is injected into the micropores of the microplate, and then the microplate is placed in an incubator for culture. During the culture process, the number of incubators will be increased or decreased at any time according to actual needs.

[0003] In the process of HPV detection, after searching, a Chinese patent with the announcement number CN216998343U discloses a microplate greenhouse culture device for HPV detection, which belongs to the field of HPV detection technology and solves the problem that it is difficult to increase or decrease the number of cultures. The microplate greenhouse culture device for HPV detection includes a storage chamber, the top and bottom sides of the storage chamber are provided with a first connecting hole, the inner wall of the first connecting hole is movably connected with a threaded rod, one end of the threaded rod is fixedly connected with a knob, the bottom inner wall of the storage chamber is movably connected with a connecting seat, and the top and bottom sides of the connecting seat are provided with a second connecting hole. Through the arrangement of the storage chamber, the threaded rod, the knob and the connecting seat, the threaded rod connects the storage chamber and the connecting seat. When the number of cultures needs to be reduced during the detection process, the knob can be turned to pull out the threaded rod, so as to achieve the effect of facilitating the disassembly of the storage chamber. When centralized cultivation is required, the connecting seat can be placed between the two storage chambers and connected with the threaded rod to achieve the purpose of centralized cultivation of the test object. However, the heating plate of the culture device is installed on the back side of the device, resulting in a large temperature difference between the front and back sides of the device, making it difficult to ensure that the test objects are cultured in the same temperature environment, which seriously affects the culture effect of the test objects and further affects the accuracy of the test results. Utility Model Content

[0004] The utility model aims to provide a microplate greenhouse culture device for HPV detection to solve the technical problems raised in the background technology.

[0005] To achieve the above-mentioned purpose, the specific technical scheme of the utility model is as follows: A microplate greenhouse culture device for HPV detection, comprising a box body, a box door is arranged on the front side of the box body, an air duct frame is arranged in the inner cavity of the box body, the air duct frame comprises two relatively distributed side wind plates and an upper wind plate located between the top ends of the two side wind plates, an opening is arranged in the middle part of the surface of the upper wind plate, an air guide seat is arranged at the opening, and a fan is arranged on the inner wall of the top end of the box body above the air guide seat, a plurality of limit strips distributed at equal intervals up and down are arranged on opposite sides of the two side wind plates, a culture plate is pull-connected between the two limit strips, the side walls of the box body are composed of an outer plate, an insulation layer, a heating plate and an inner plate from the outside to the inside, and a temperature sensor is arranged in the inner cavity of the box body.

[0006] Preferably, supporting feet are arranged at the four corners of the bottom of the box body, and slots corresponding to the supporting feet are arranged at the four corners of the upper surface of the box body, and the size of the supporting feet matches the size of the slots.

[0007] Preferably, an observation window is provided on the surface of the door, and a controller is provided on the surface of the door above the observation window, and display-level control buttons are respectively provided on the surface of the controller.

[0008] Preferably, the side wind plates and upper wind plates are both U-shaped structures, and air ducts are formed between the U-shaped openings of the side wind plates and the upper wind plates and the inner wall of the box body. U-shaped openings are provided in the middle parts of the upper and lower ends of the side wind plates, and the upper wind plates are installed at the U-shaped openings located above.

[0009] Preferably, the air guide seat is a cone structure with a large bottom and a small top, and the air guide seat is a cavity structure with an opening at the bottom, and the front and rear side walls of the air guide seat are both provided with a plurality of long strip openings that are evenly and orderly distributed.

[0010] Preferably, the surface of the culture plate is provided with a plurality of culture grooves evenly and orderly distributed, and a plurality of ventilation holes equally spaced are provided in the middle part of the surface of the culture plate, trapezoidal bars are provided on both sides of the culture plate, and the side walls of the limiting bars are provided with trapezoidal grooves slidably connected to the trapezoidal bars.

[0011] Preferably, the controller is electrically connected to the heating plate and the temperature sensor respectively.

[0012] The utility model of a microplate greenhouse culture device for HPV detection has the following advantages:

[0013] 1. Under the action of the wind guide seat, the utility model allows the wind to flow through the upper wind plate to the air duct formed by the side wind plates on both sides and the inner wall of the box, and enters the bottom between the two side wind plates through the U-shaped opening at the bottom, and flows upward through the vents on the culture plate, and finally flows to the side wind plates on the left and right sides through the wind guide seat, thereby realizing air circulation in the inner cavity of the box, ensuring uniform temperature inside the box, ensuring that each culture plate is cultured in the same environment, and facilitating the correctness of the test results.

[0014] 2. With the cooperation between the trapezoidal bars and the trapezoidal grooves, the culture plates can be easily installed and disassembled, and the operation is simple. Since the side wind plate is provided with a plurality of limit bars evenly spaced up and down, it is convenient to place a plurality of culture plates at one time for culture, and the spacing between the upper and lower culture plates can be adjusted, which has strong flexibility.

[0015] 3. Through the cooperation between the supporting feet and the slots, multiple boxes can be stacked up and down, which greatly saves space. By setting the insulation layer, the heat loss in the heating plate can be reduced. In addition, since heating plates are set on the back, left and right sides and the upper side of the box, the uniformity of heat inside the box is guaranteed. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the structure inside the box in the utility model;

[0019] Figure 3 It is a structural schematic diagram of the wind channel frame in the utility model;

[0020] Figure 4 for Figure 3 A schematic diagram of the structure from another perspective;

[0021] Figure 5 It is a structural schematic diagram of the culture plate in the utility model;

[0022] Figure 6 It is a schematic diagram of the structure of the side wall of the box body in the utility model;

[0023] Explanation of the markings in the figure: 1. Box body; 2. Box door; 3. Support feet; 4. Slot; 5. Observation window; 6. Controller; 7. Display screen; 8. Control button; 9. Duct rack; 10. Culture plate; 11. Side wind plate; 12. Upper wind plate; 13. U-shaped opening; 14. Limiting strip; 15. Trapezoidal slot; 16. Air guide seat; 17. Long strip opening; 18. Trapezoidal strip; 19. Culture trough; 20. Ventilation port; 21. Outer plate; 22. Insulation layer; 23. Heating plate; 24. Inner plate. DETAILED DESCRIPTION

[0024] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the embodiments of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0025] In the description of the embodiments of the present invention, it should be understood that the terms "length", "vertical", "horizontal", "top", "bottom", etc. indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the embodiments of the present invention.

[0026] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the embodiments of the present utility model, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0027] In the embodiments of the present utility model, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present utility model can be understood according to specific circumstances.

[0028] The disclosure below provides many different embodiments or examples for realizing different structures of the embodiments of the present utility model. In order to simplify the disclosure of the embodiments of the present utility model, the components and settings of specific examples are described below. Of course, they are only examples, and the purpose is not to limit the embodiments of the present utility model. In addition, the embodiments of the present utility model can repeat reference numbers and / or reference letters in different examples, and this repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed.

[0029] In order to better understand the purpose, structure and function of the utility model, the utility model is further described in detail below with reference to the accompanying drawings, in a microplate greenhouse culture device for HPV detection.

[0030] like Figure 1-6As shown, a microplate greenhouse culture device for HPV detection of the utility model includes a box body 1, and support feet 3 are provided at the four corners of the bottom of the box body 1. Slots 4 corresponding to the support feet 3 are provided at the four corners of the upper surface of the box body 1. The size of the support feet 3 matches the size of the slots 4. Through the cooperation between the support feet 3 and the slots 4, multiple boxes 1 can be stacked up and down, which greatly saves space. A box door 2 is provided on the front side of the box body 1, and an observation window 5 is provided on the surface of the box door 2. A controller 6 is provided on the surface of the box door 2 above the observation window 5. A display screen 7 and control buttons 8 are provided on the surface of the controller 6. Through the setting of the observation window 5, it is convenient to observe the culture situation of the inner cavity of the box body 1, and through the setting of the display screen 7, it is convenient to observe the temperature of the inner cavity of the box body 1, and under the action of the control button 8, it is convenient to operate. The inner cavity of the box body 1 is provided with an air duct frame 9, and the air duct frame 9 includes two relatively distributed side air plates 11 and an upper air plate 12 located between the top ends of the two side air plates 11. An opening is provided in the middle part of the surface of the upper air plate 12, and an air guide seat 16 is provided at the opening. A fan is provided at the top inner wall of the box body 1 located above the air guide seat 16. The side air plates 11 and the upper air plates 12 are both U-shaped structures, and an air duct is formed between the U-shaped openings of the side air plates 11 and the upper air plates 12 and the inner wall of the box body 1. U-shaped openings 13 are provided in the middle parts of the upper and lower ends of the side air plates 11, and the upper air plate 12 is installed at the U-shaped opening 13 located above , the fan starts to generate wind flow, and under the action of the wind guide seat 16, the wind flow passes through the upper wind plate 12 to the air duct formed by the side wind plates 11 on both sides and the inner wall of the box 1, and enters the bottom between the two side wind plates 11 through the U-shaped opening 13 at the bottom, and flows upward through the vents 20 on the culture plate 10, and finally flows to the side wind plates 11 on the left and right sides through the wind guide seat 16, thereby realizing the air circulation flow in the inner cavity of the box 1, ensuring the uniform temperature inside the box 1, and ensuring that each culture plate 10 is cultured in the same environment, which is conducive to ensuring the correctness of the test results. The wind guide seat 16 is a cone structure with a large bottom and a small top, and the wind guide seat 16 is a cavity structure with an opening at the bottom. The front and rear side walls of the wind guide seat 16 are provided with a plurality of evenly and orderly distributed long strip openings 17, which is conducive to the upward wind flow entering the upper part of the upper wind plate 12 through the long strip openings 17, and at the same time, the wind flow blown out by the fan flows to the left and right sides.

[0031] A plurality of limit bars 14 equidistantly spaced from top to bottom are provided on opposite sides of the two side wind plates 11, a culture plate 10 is connected by pulling between the two limit bars 14, a plurality of culture grooves 19 evenly and orderly distributed are provided on the surface of the culture plate 10, and a plurality of equidistantly distributed ventilation holes 20 are provided in the middle part of the surface of the culture plate 10, trapezoidal bars 18 are provided on both sides of the culture plate 10, and a trapezoidal groove 15 slidably connected to the trapezoidal bars 18 is provided on the side wall of the limit bar 14, the culture groove 19 is used to place the test object, and the setting of the ventilation holes 20 is conducive to the air in the inner cavity of the box body 1 to flow from bottom to top, and with the cooperation between the trapezoidal bars 18 and the trapezoidal grooves 15, the culture plate 10 is convenient to install and disassemble, and the operation is simple, because the side wind plate 11 is provided with a plurality of limit bars 14 equidistantly spaced from top to bottom, so that multiple culture plates 10 can be placed for culture at one time, and the spacing between the upper and lower culture plates 10 can be adjusted, which has strong flexibility. The side walls of the box body 1 are composed of an outer plate 21, an insulation layer 22, a heating plate 23 and an inner plate 24 from the outside to the inside. The setting of the insulation layer 22 can reduce the heat loss in the heating plate 23. Since the rear side, left and right sides and the upper side of the box body 1 are provided with heating plates 23, the uniformity of heat inside the box body 1 is ensured. A temperature sensor is provided in the inner cavity of the box body 1, and the controller 6 is electrically connected to the heating plate 23 and the temperature sensor respectively, which is convenient for controlling the temperature of the inner cavity of the box body 1 to ensure that the detected object is in a suitable temperature environment.

[0032] It is understood that the present invention is described by some embodiments, and those skilled in the art are aware that various changes or equivalent substitutions may be made to these features and embodiments without departing from the spirit and scope of the present invention. In addition, under the teachings of the present invention, these features and embodiments may be modified to adapt to specific circumstances and materials without departing from the spirit and scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the scope of protection of the present invention.

Claims

1. A microplate greenhouse culture device for HPV detection, characterized in that: The invention comprises a box body (1), wherein a box door (2) is arranged on the front side of the box body (1), and an air duct frame (9) is arranged in the inner cavity of the box body (1), wherein the air duct frame (9) comprises two side air plates (11) which are arranged opposite to each other, and an upper air plate (12) which is located between the top ends of the two side air plates (11), wherein an opening is arranged in the middle part of the surface of the upper air plate (12), and an air guide seat (16) is arranged at the opening, and a fan is arranged on the inner wall of the top end of the box body (1) which is located above the air guide seat (16), and a plurality of limit strips (14) which are arranged at equal intervals up and down are arranged on opposite sides of the two side air plates (11), and a culture plate (10) is connected by pulling between the two limit strips (14), and the side walls of the box body (1) are composed of an outer plate (21), a heat preservation layer (22), a heating plate (23) and an inner plate (24) from the outside to the inside, and a temperature sensor is arranged in the inner cavity of the box body (1).

2. A microplate greenhouse culture device for HPV detection according to claim 1, characterized in that: Support feet (3) are arranged at the four corners of the bottom of the box (1), and slots (4) corresponding to the support feet (3) are arranged at the four corners of the upper surface of the box (1), and the size of the support feet (3) matches the size of the slots (4).

3. The microplate greenhouse culture device for HPV detection according to claim 1, characterized in that: An observation window (5) is provided on the surface of the box door (2), and a controller (6) is provided on the surface of the box door (2) above the observation window (5), and a display screen (7) and control buttons (8) are provided on the surface of the controller (6).

4. The microplate greenhouse culture device for HPV detection according to claim 1, characterized in that: The side wind plate (11) and the upper wind plate (12) are both U-shaped structures, and an air duct is formed between the U-shaped openings of the side wind plate (11) and the upper wind plate (12) and the inner wall of the box body (1). The middle parts of the upper and lower ends of the side wind plate (11) are both provided with U-shaped openings (13), and the upper wind plate (12) is installed at the U-shaped opening (13) located above.

5. The microplate greenhouse culture device for HPV detection according to claim 1, characterized in that: The air guide seat (16) is a cone structure with a large bottom and a small top, and the air guide seat (16) is a cavity structure with an open bottom. The front and rear side walls of the air guide seat (16) are both provided with a plurality of evenly and orderly distributed long strip openings (17).

6. The microplate greenhouse culture device for HPV detection according to claim 1, characterized in that: The surface of the culture plate (10) is provided with a plurality of culture grooves (19) distributed evenly and orderly, and a plurality of ventilation holes (20) distributed at equal intervals are provided in the middle of the surface of the culture plate (10), both sides of the culture plate (10) are provided with trapezoidal bars (18), and the side walls of the limiting bars (14) are provided with trapezoidal grooves (15) slidably connected to the trapezoidal bars (18).

7. The microplate greenhouse culture device for HPV detection according to claim 3, characterized in that: The controller (6) is electrically connected to the heating plate (23) and the temperature sensor respectively.

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