Treatment device for cell culture waste liquid

Abstract

The utility model discloses a treatment device for cell culture waste liquid, which relates to the technical field of treatment of cell culture waste liquid and comprises a U-shaped frame and a waste liquid treatment component, the top of the waste liquid treatment component is movably connected with a cover plate, and the top of the cover plate and the bottoms of the outer walls of two guide pipes penetrate to the bottom of the cover plate. Liquid storage assemblies are arranged on the left side and the right side of the top of the transverse face of the U-shaped frame correspondingly, connecting pipes are fixedly connected to the bottoms of the two liquid storage assemblies correspondingly, liquid injection pipes are fixedly connected to the left side and the right side of the front face of the waste liquid treatment assembly correspondingly, and liquid discharging pipes are fixedly connected to the left side and the right side of the bottom of the waste liquid treatment assembly correspondingly. According to the utility model, the connecting pipe and the guide pipe are connected and fixed by using the bolt, a sodium hydroxide solution is put into the alkaline sterilization tank, and a hydrochloric acid solution and a disinfectant are put into the acidic sterilization tank, so that different sterilization solutions are selected for cell species properties in two reaction barrels in the waste liquid treatment assembly.

Description

Technical Field

[0001] The utility model relates to the technical field of treatment of cell culture waste liquid, in particular to a treatment device for cell culture waste liquid. Background Art

[0002] Cell culture refers to a method of simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable cells to survive, grow, reproduce and maintain their main structures and functions. Cell culture technology is an important and commonly used technology in cell biology research methods. Through cell culture, a large number of cells can be obtained, and cell signal transduction, cell anabolism, cell growth and proliferation, etc. can be studied. After use, waste liquids such as cell fluid and culture fluid need to be discarded, and a treatment device is required to collect and treat the waste liquid. The following problems exist in the existing technology:

[0003] The existing waste liquids from cell cultures of different natures contain different amounts of chemical agents. Unified recovery leads to bacterial growth in the waste liquid, thereby increasing the possibility of bacterial, fungal and cell culture contamination. Secondly, during the collection of cell culture waste liquid, liquid splashing is likely to occur, and after the treatment is completed, it is inconvenient to clean the device. Utility Model Content

[0004] The utility model provides a device for treating cell culture waste liquid to solve the problems existing in the above-mentioned background technology.

[0005] In order to solve the above technical problems, the technical solution adopted by the utility model is:

[0006] A device for treating cell culture waste liquid comprises a U-shaped frame and a waste liquid treatment component, wherein the top of the waste liquid treatment component is movably connected with a cover plate, and conduits are respectively arranged on the left and right sides of the top of the cover plate, and the outer wall bottoms of the two conduits penetrate to the bottom of the cover plate, and liquid storage components are respectively arranged on the left and right sides of the top of the transverse surface of the U-shaped frame, and the bottoms of the two liquid storage components are fixedly connected with connecting pipes, and the outer wall bottoms of the two connecting pipes penetrate the upper and lower sides of the top of the transverse surface of the U-shaped frame and are movably connected to the tops of the two conduits, the left and right sides of the front of the waste liquid treatment component are respectively fixedly connected with injection pipes, and the left and right sides of the bottom of the waste liquid treatment component are respectively fixedly connected with drainage pipes, and the opposite sides of the U-shaped frame in the vertical direction are fixedly connected with a support seat, and the outer wall bottom of the waste liquid treatment component is arranged inside the support seat.

[0007] A further improvement of the technical solution of the utility model lies in that: the liquid storage component comprises an alkaline sterilization tank and an acidic sterilization tank, the fronts of the alkaline sterilization tank and the acidic sterilization tank are respectively provided with visual windows, the tops of the alkaline sterilization tank and the acidic sterilization tank are respectively fixedly connected with liquid filling ports, the bottoms of the two connecting pipes are fixedly connected with flange 1, the outer walls of the two connecting pipes are respectively provided with manual pipe valves, and the two manual pipe valves penetrate into the interior of the connecting pipes.

[0008] A further improvement of the technical solution of the utility model is that a mounting groove is provided on the top of the support seat, and connecting plates are fixedly connected to the left and right sides of the outer wall of the support seat respectively, and one end of the two connecting plates away from the support seat is fixedly connected to the inner wall of the U-shaped frame in the vertical direction.

[0009] A further improvement of the technical solution of the utility model is that: a threaded pipe is fixedly connected to the bottom of the cover plate, a plurality of protrusions are fixedly connected to the outer wall of the cover plate in an annular array, and flange 2 is fixedly connected to the tops of the two conduits.

[0010] A further improvement of the technical solution of the utility model is that the waste liquid treatment component includes a waste liquid collection tank, a threaded groove is provided on the top of the outer wall of the waste liquid collection tank, reaction barrels are respectively provided on the left and right sides of the inner wall of the waste liquid collection tank, and the tops of the inner walls of the two reaction barrels are movably connected with splash-proof components.

[0011] A further improvement of the technical solution of the utility model is that the reaction barrel and the splash-proof component are both arranged in a semicircular shape.

[0012] A further improvement of the technical solution of the utility model is that the splash-proof assembly includes a splash-proof box, a circular array of connecting holes one extending vertically through the bottom of the inner wall of the splash-proof box is provided, a circular array of connecting holes two extending inside and outside are provided on the arc-shaped outer wall of the splash-proof box, and L-shaped clips are fixedly connected to the rear and rear sides of the horizontal surface of the splash-proof box, and anti-slip pads are provided on the inner walls of the two L-shaped clips in the vertical direction.

[0013] A further improvement of the technical solution of the utility model lies in that: rubber strips are fixedly connected to the left and right sides of the top of the inner wall of the waste liquid collection tank, the reaction barrel is arranged at the bottom of the rubber strip, and a liquid inlet groove that passes through the inside and outside is opened on the side of the reaction barrel close to the injection pipe, and handles are fixed on the front and back sides of the opposite tops of the two reaction barrels.

[0014] Due to the adoption of the above technical solution, the utility model has achieved the following technical progress compared with the prior art:

[0015] 1. The utility model provides a treatment device for cell culture waste liquid, which adopts a U-shaped frame, a waste liquid treatment component, a cover plate, a liquid storage component, a connecting pipe, a catheter, a liquid injection pipe, a liquid discharge pipe and a support seat. The waste liquid treatment component is installed on the support seat, and the connecting pipe and the catheter are connected and fixed with bolts. A sodium hydroxide solution is placed in an alkaline sterilization tank, and a hydrochloric acid solution and a disinfectant are placed in an acidic sterilization tank, so that different sterilization solutions are selected according to the properties of the cell species in the two reaction barrels in the waste liquid treatment component, which solves the problem of different contents of chemical agents in the existing waste liquids of cell cultures of different properties, and the problem of uniform recovery leading to bacterial growth in the waste liquid, thereby increasing the possibility of bacterial, fungal and cell culture contamination, and achieving the beneficial effect of facilitating the classification and treatment of cell culture waste liquids of different properties.

[0016] 2. The utility model provides a treatment device for cell culture waste liquid, which adopts the cooperation between a reaction barrel, a handle, a splash-proof component and a rubber strip, and buffers the incoming waste liquid and solution through the connecting hole 1 and the connecting hole 2 in the two splash-proof boxes to avoid liquid splashing. After the treatment is completed, the two reaction barrels are taken out from the waste liquid collection tank for cleaning through the handle, which solves the problem that liquid splashing is easy to occur during the collection of cell culture waste liquid, and it is inconvenient to clean the device after the treatment is completed, thereby achieving the beneficial effect of improving the use effect of the treatment device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional structural schematic diagram of the cell culture waste liquid treatment device of the utility model;

[0018] Figure 2 This is a schematic diagram of the three-dimensional structure of the U-shaped frame of the utility model;

[0019] Figure 3 It is a schematic diagram of the three-dimensional structure of the waste liquid treatment component and the cover plate of the utility model;

[0020] Figure 4 This is a schematic diagram of the three-dimensional structure of the splash-proof component of the utility model

[0021] Figure 5 It is a schematic diagram of the three-dimensional structure of the reaction barrel of the utility model.

[0022] In the figure: 1. U-shaped frame; 2. Waste liquid treatment component; 21. Waste liquid collection tank; 210. Threaded groove; 22. Reaction barrel; 220. Liquid inlet groove; 221. Handle; 23. Splash-proof component; 230. Connecting hole one; 231. Splash-proof box; 232. Connecting hole two; 233. L-shaped card plate; 234. Anti-skid pad; 24. Rubber strip; 3. Cover plate; 31. Threaded pipe; 32. Bump; 4. Liquid storage component; 41. Alkaline sterilization tank; 42. Acid sterilization tank; 43. Visual window; 44. Liquid filling port; 5. Connecting pipe; 51. Flange one; 52. Manual pipe valve; 6. Conduit; 61. Flange two; 7. Liquid filling pipe; 8. Liquid discharge pipe; 9. Support seat; 901. Mounting groove; 91. Connecting plate. DETAILED DESCRIPTION

[0023] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods:

[0024] like Figure 1 As shown, the utility model provides a treatment device for cell culture waste liquid, including a U-shaped frame 1 and a waste liquid treatment component 2, the top of the waste liquid treatment component 2 is movably connected with a cover plate 3, the left and right sides of the top of the cover plate 3 are respectively provided with conduits 6, and the outer wall bottoms of the two conduits 6 penetrate to the bottom of the cover plate 3, the left and right sides of the top of the transverse surface of the U-shaped frame 1 are respectively provided with liquid storage components 4, the bottoms of the two liquid storage components 4 are fixedly connected with connecting pipes 5, and the outer wall bottoms of the two connecting pipes 5 penetrate the upper and lower sides of the top of the transverse surface of the U-shaped frame 1 and are movably connected to the tops of the two conduits 6, the left and right sides of the front of the waste liquid treatment component 2 are respectively fixedly connected with injection pipes 7, the left and right sides of the bottom of the waste liquid treatment component 2 are respectively fixedly connected with drainage pipes 8, the opposite side of the U-shaped frame 1 in the vertical direction is fixedly connected with a support seat 9, and the outer wall bottom of the waste liquid treatment component 2 is arranged inside the support seat 9;

[0025] A U-shaped frame 1, a waste liquid treatment component 2, a cover plate 3, a liquid storage component 4, a connecting pipe 5, a conduit 6, an injection pipe 7, a discharge pipe 8, and a support seat 9 are provided. The waste liquid treatment component 2 is supported by the support seat 9, the connecting pipe 5 and the conduit 6 are connected and fixed by bolts, and the alkaline sterilization tank 41 and the acidic sterilization tank 42 in the liquid storage component 4 are respectively stored with sodium hydroxide solution, hydrochloric acid solution, and disinfectant sterilization solution according to the different properties of cell culture waste liquid, so that they correspond to the left and right reaction barrels 22 in the waste liquid treatment component 2, and the waste liquid in the reaction barrel 22 is sterilized.

[0026] like Figure 2As shown, the utility model provides a technical solution for a treatment device of cell culture waste liquid: the liquid storage component 4 includes an alkaline sterilization tank 41 and an acidic sterilization tank 42, the front of the alkaline sterilization tank 41 and the acidic sterilization tank 42 are respectively provided with a visual window 43, the top of the alkaline sterilization tank 41 and the acidic sterilization tank 42 are respectively fixedly connected with a liquid injection port 44, and solutions of different properties are stored in the alkaline sterilization tank 41 and the acidic sterilization tank 42 through the liquid injection port 44, and their capacities can be observed through the visual window 43 The bottoms of the two connecting pipes 5 are fixedly connected with flanges 51, the outer walls of the two connecting pipes 5 are provided with manual pipe valves 52, and the two manual pipe valves 52 penetrate into the interior of the connecting pipes 5. The top of the support seat 9 is provided with a mounting groove 901, and the left and right sides of the outer wall of the support seat 9 are respectively fixedly connected with connecting plates 91, and the ends of the two connecting plates 91 away from the support seat 9 are fixedly connected to the inner wall of the U-shaped frame 1 in the vertical direction, and the bottom of the waste liquid treatment component 2 is placed in the mounting groove 901 for support.

[0027] like Figure 3 As shown, the utility model provides a technical solution for a treatment device of cell culture waste liquid: a threaded tube 31 is fixedly connected to the bottom of the cover plate 3, a plurality of protrusions 32 are fixedly connected to the outer wall of the cover plate 3 in an annular array, the tops of the two conduits 6 are fixedly connected to flanges 61, the waste liquid treatment component 2 comprises a waste liquid collecting tank 21, a threaded groove 210 is provided on the top of the outer wall of the waste liquid collecting tank 21, the inner wall of the threaded tube 31 is screwed with the outer wall of the threaded groove 210, so that the cover plate 3 is installed on the waste liquid collecting tank 21, reaction barrels 22 are respectively arranged on the left and right sides of the inner wall of the waste liquid collecting tank 21, the inner wall tops of the two reaction barrels 22 are movably connected with splash-proof components 23, the reaction barrels 22 and the splash-proof components 23 are both arranged in a semicircular shape, and two reaction barrels 22 are installed in the waste liquid collecting tank 21, so that cell culture waste liquids of different properties can be collected, thereby improving the diversity of collection.

[0028] like Figure 4 As shown, the utility model provides a technical solution for a treatment device of cell culture waste liquid: the splash-proof component 23 includes a splash-proof box 231, a circular array at the bottom of the inner wall of the splash-proof box 231 is provided with a connecting hole 1 230 that passes through from top to bottom, and a circular array on the arc-shaped outer wall of the splash-proof box 231 is provided with a connecting hole 232 that passes through inside and outside, and the liquid introduced through the connecting hole 1 230 and the connecting hole 2 232 can be dispersed to play a buffering role to avoid splashing, and the horizontal front and rear sides of the splash-proof box 231 are respectively fixedly connected with L-shaped card plates 233, and the vertical inner walls of the two L-shaped card plates 233 are provided with anti-skid pads 234, and the splash-proof box 231 can be installed into the interior of the reaction barrel 22 through the L-shaped card plates 233 and the anti-skid pads 234.

[0029] like Figure 5As shown, the utility model provides a technical solution for a treatment device of cell culture waste liquid: rubber strips 24 are fixedly connected to the left and right sides of the top of the inner wall of the waste liquid collection tank 21, and the reaction barrel 22 is arranged at the bottom of the rubber strip 24. The reaction barrel 22 can be limited by the rubber strip 24. A liquid inlet groove 220 that passes through the inside and outside is opened on the side of the reaction barrel 22 close to the injection tube 7. Handles 221 are fixed to the front and back sides of the opposite tops of the two reaction barrels 22, respectively. The two reaction barrels 22 can be drawn out of the waste liquid collection tank 21 by the handles 221.

[0030] The working principle of the device for treating cell culture waste liquid will be described in detail below.

[0031] like Figure 1-5 As shown, when the cell culture waste liquid is processed, first, the reaction barrel 22 is placed into the waste liquid collection tank 21 through the handle 221, so that the rubber strip 24 limits the surface of the waste liquid collection tank 21, and then the two splash-proof boxes 231 are placed in the two reaction barrels 22 respectively, and are clamped at the outer ends of the reaction barrels 22 through the L-shaped clamping plate 233, so that the anti-slip pad 234 is tightly fitted to the outer wall of the reaction barrel 22 for fixation, and then the threaded tube 31 in the cover plate 3 is screwed and connected with the threaded groove 210 on the waste liquid collection tank 21, and the bottom of the waste liquid treatment component 2 is placed in the installation groove 901 in the support seat 9, so as to support and position the waste liquid treatment component 2, and then the flange 1 51 at the bottom of the connecting pipe 5 is installed and connected with the flange 2 61 at the top of the conduit 6 by bolts, and then the alkali A sterilizing solution of sodium hydroxide solution is placed in the alkaline sterilization tank 41, and a sterilizing solution of hydrochloric acid solution and disinfectant is placed in the acidic sterilization tank 42. Then, the cell culture waste liquids of different properties are respectively introduced into the corresponding reaction barrels 22 along the liquid inlet groove 220 through the injection pipe 7. At the same time, the cell waste liquid flows into the reaction barrel 22 through the connecting hole 230 and the splash-proof box 231 to avoid splashing. Then, the two manual pipe valves 52 are opened in sequence, so that the sterilizing solutions in the alkaline sterilization tank 41 and the acidic sterilization tank 42 are respectively introduced into the corresponding reaction barrels 22 through the connecting pipe 5 for sterilization treatment. After a period of time, the waste liquid is discharged by opening the drain pipe 8. After the waste liquid is treated, the reaction barrel 22 is taken out and cleaned by opening the cover plate 3 and pulling the handle 221 upward.

[0032] The above generally describes the present invention in detail, but it is obvious to a person skilled in the art that some modifications or improvements can be made to the present invention. Therefore, modifications or improvements that do not deviate from the spirit of the present invention are within the scope of protection of the present invention.

Claims

1. A cell culture waste liquid treatment device, comprising a U-shaped frame (1) and a waste liquid treatment component (2), characterized in that: The top of the waste liquid treatment component (2) is movably connected to a cover plate (3), and the left and right sides of the top of the cover plate (3) are respectively provided with conduits (6), and the outer wall bottoms of the two conduits (6) penetrate to the bottom of the cover plate (3). The left and right sides of the top of the transverse surface of the U-shaped frame (1) are respectively provided with liquid storage components (4), and the bottoms of the two liquid storage components (4) are fixedly connected to connecting pipes (5), and the outer wall bottoms of the two connecting pipes (5) penetrate the upper and lower sides of the top of the transverse surface of the U-shaped frame (1) and are movably connected to the tops of the two conduits (6). The left and right sides of the front of the waste liquid treatment component (2) are respectively fixedly connected to injection pipes (7), and the left and right sides of the bottom of the waste liquid treatment component (2) are respectively fixedly connected to drainage pipes (8). The opposite sides of the U-shaped frame (1) in the vertical direction are fixedly connected to a support seat (9), and the outer wall bottom of the waste liquid treatment component (2) is arranged inside the support seat (9).

2. The device for treating cell culture waste liquid according to claim 1, characterized in that: The liquid storage assembly (4) comprises an alkaline sterilization tank (41) and an acidic sterilization tank (42), the front faces of the alkaline sterilization tank (41) and the acidic sterilization tank (42) are respectively provided with visual windows (43), the tops of the alkaline sterilization tank (41) and the acidic sterilization tank (42) are respectively fixedly connected with liquid injection ports (44), the bottoms of the two connecting pipes (5) are respectively fixedly connected with flanges 1 (51), the outer walls of the two connecting pipes (5) are respectively provided with manual pipe valves (52), and the two manual pipe valves (52) penetrate into the interior of the connecting pipes (5).

3. The device for treating cell culture waste liquid according to claim 1, characterized in that: The top of the support seat (9) is provided with a mounting groove (901), and the left and right sides of the outer wall of the support seat (9) are respectively fixedly connected with connecting plates (91), and the ends of the two connecting plates (91) away from the support seat (9) are fixedly connected to the inner wall of the U-shaped frame (1) in the vertical direction.

4. The device for treating cell culture waste liquid according to claim 1, characterized in that: The bottom of the cover plate (3) is fixedly connected to a threaded tube (31), the outer wall of the cover plate (3) is fixedly connected to a plurality of protrusions (32) in an annular array, and the tops of the two conduits (6) are fixedly connected to flange 2 (61).

5. The device for treating cell culture waste liquid according to claim 1, characterized in that: The waste liquid treatment component (2) comprises a waste liquid collection tank (21), the top of the outer wall of the waste liquid collection tank (21) is provided with a threaded groove (210), the left and right sides of the inner wall of the waste liquid collection tank (21) are respectively provided with reaction barrels (22), and the tops of the inner walls of the two reaction barrels (22) are movably connected with splash-proof components (23).

6. The device for treating cell culture waste liquid according to claim 5, characterized in that: The reaction barrel (22) and the splash-proof assembly (23) are both arranged in a semicircular shape.

7. The device for treating cell culture waste liquid according to claim 5, characterized in that: The splash-proof assembly (23) comprises a splash-proof box (231), the inner wall bottom of the splash-proof box (231) is provided with a circular array of communicating holes (230) that pass through from top to bottom, the arc-shaped outer wall of the splash-proof box (231) is provided with a circular array of communicating holes (232) that pass through from inside to outside, and the horizontal front and rear sides of the splash-proof box (231) are respectively fixedly connected with L-shaped clamping plates (233), and the inner walls of the two L-shaped clamping plates (233) in the vertical direction are both provided with anti-slip pads (234).

8. The device for treating cell culture waste liquid according to claim 5, characterized in that: The left and right sides of the inner wall top of the waste liquid collection tank (21) are respectively fixedly connected with rubber strips (24); the reaction barrel (22) is arranged at the bottom of the rubber strip (24); a liquid inlet groove (220) that penetrates inside and outside is opened on one side of the reaction barrel (22) close to the liquid injection pipe (7); and handles (221) are respectively fixed on the front and rear sides of the tops of the opposite surfaces of the two reaction barrels (22).

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