Protein purification equipment

Abstract

The utility model discloses protein purification equipment, which comprises a purification equipment main body, a mechanical arm, a movable block, a filler, a fixed seat and a test tube rack, a first sliding chute is arranged on the purification equipment main body, the mechanical arm is arranged on the first sliding chute in a sliding manner, and the mechanical arm is connected with a first power mechanism; a second sliding groove is formed in the mechanical arm, the moving block is slidably installed on the second sliding groove, the moving block is connected with a second power mechanism, and the filler is fixedly installed on the moving block; a fixing seat is mounted on the purification equipment main body; a containing groove is formed in the top end of the fixing seat; the test tube rack comprises a placing plate and a limiting plate, the placing plate is supported by the top end of the fixed seat, the limiting plate is located in the accommodating groove, and the groove wall of the accommodating groove is in contact with the limiting plate; a plurality of through holes are distributed in the test tube rack, and a rubber sleeve is fixed on the hole wall of each through hole. The test tube rack disclosed by the utility model can be placed on the fixed seat and moved away from the fixed seat, so that all test tubes on the test tube rack can be integrally taken and placed, and the working efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of protein purification, and more specifically relates to a protein purification device. Background Art

[0002] To study a certain protein, it is necessary to first purify the protein from other proteins and non-protein molecules. The low-temperature organic solvent precipitation method uses water-miscible organic solvents such as methanol and ethanol to reduce the solubility of most proteins and precipitate them. Compared with salting out, this method has a higher resolution.

[0003] In the process of protein purification, samples are generally placed in multiple test tubes, which are then inserted into the protein purification equipment. Then, a movable robotic arm and a filler on the robotic arm are used to inject the organic solvent that can precipitate the protein into the test tubes.

[0004] A commonly used protein purifier has a test tube hole on its main body. A large number of test tubes are usually placed in the test tube hole. The test tubes need to be taken out and put in one by one, which wastes the working time of the protein purifier and affects the working efficiency of the protein purifier. Utility Model Content

[0005] In view of the above defects or improvement needs of the prior art, the utility model provides a protein purification device, in which a test tube rack can be conveniently placed on and removed from a fixed seat, and all test tubes on the test tube rack can be taken and placed as a whole, thereby improving the working efficiency of the protein purification device.

[0006] To achieve the above-mentioned purpose, according to the utility model, a protein purification device is provided, comprising a purification device body, a mechanical arm, a moving block, a filler, a fixing seat and a test tube rack, characterized in that:

[0007] The purification equipment body is provided with a horizontal first slide groove, the mechanical arm is slidably mounted on the first slide groove, and the mechanical arm is connected to a first power mechanism to slide along the first slide groove under the drive of the first power mechanism;

[0008] The mechanical arm is provided with a horizontal second slide groove, the moving block is slidably mounted on the second slide groove, the moving block is connected to a second power mechanism so as to slide along the second slide groove under the drive of the second power mechanism, the filler is fixedly mounted on the moving block, the filler is connected to the pump body through a hose, and the second slide groove is perpendicular to the first slide groove;

[0009] The fixing seat is installed on the main body of the purification equipment and a receiving groove is arranged on the top of the fixing seat;

[0010] The test tube rack comprises a placing plate and a limiting plate fixedly connected together, the placing plate and the limiting plate are both horizontal, and the placing plate is located above the limiting plate, the placing plate is located outside the receiving groove and is supported by the top end of the fixing seat, the limiting plate is located in the receiving groove, and the groove wall of the receiving groove contacts the limiting plate to limit the horizontal movement of the limiting plate;

[0011] The test tube rack is provided with a plurality of through holes for inserting test tubes, and a rubber sleeve is fixed on the hole wall of each through hole to prevent the inserted test tube from falling.

[0012] Preferably, the accommodating groove and the limiting plate are both in a rectangular parallelepiped shape, and each side surface of the limiting plate is in contact with a groove wall of the accommodating groove.

[0013] Preferably, a plurality of handles are provided on the top of the placement plate.

[0014] Preferably, the placement plate and the limiting plate are integrally formed.

[0015] Preferably, the through holes on the test tube rack are arranged in an array.

[0016] Preferably, the rubber sleeve is in the shape of a hollow cylinder.

[0017] Preferably, there are multiple fillers on the moving block so as to simultaneously fill multiple test tubes with organic solvent.

[0018] Preferably, a refrigeration device is provided on the fixing seat to cool the test tube in the containing tank.

[0019] Preferably, the placement plate completely covers the accommodating groove.

[0020] Preferably, the moving block is exposed from the robot arm, the filler is installed inside the moving block, and the lower end of the filler is exposed from the moving block.

[0021] In general, the above technical solutions conceived by the utility model can achieve the following beneficial effects compared with the prior art:

[0022] The utility model discloses a protein purification device, wherein a test tube rack is installed on a fixed seat, and a placement plate of the test tube rack is supported by the fixed seat, so that the test tube rack and all test tubes can be taken out and placed as a whole. The limit plate of the test tube rack is placed in a receiving groove and abuts against the groove wall of the receiving groove, which can effectively prevent the test tube rack and the test tube from shaking during operation, so that the protein purification device can work stably. In addition, a rubber sleeve is provided at the through hole of the test tube rack, and the rubber sleeve can be elastically deformed to clamp the test tube, so as to prevent the test tube from falling when the test tube rack is taken out and placed as a whole. The utility model can greatly save the working time of the protein purification device, thereby improving the protein purification efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of a test tube plugged into a protein purification device of the utility model;

[0024] Figure 2 It is a schematic diagram of a protein purification device of the utility model without the test tube rack;

[0025] Figure 3 It is a schematic diagram of inserting test tubes on the test tube rack of the utility model;

[0026] Figure 4 It is a schematic diagram of a test tube rack in the utility model. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. In addition, the technical features involved in each embodiment of the utility model described below can be combined with each other as long as they do not conflict with each other.

[0028] Reference Figure 1 to Figure 4 A protein purification device comprises a purification device body 1, a mechanical arm 102, a moving block 103, a filler 101, a fixing seat 2 and a test tube rack 30. The purification device body 1 is the main structure of the protein purification device, and has a device housing and electrical components arranged in the device housing.

[0029] A horizontal first slide groove is arranged on the purification equipment body 1, and the robot arm 102 is slidably installed on the first slide groove. The robot arm 102 is connected to a first power mechanism to slide along the first slide groove under the drive of the first power mechanism; the first power mechanism is preferably arranged on the purification equipment body 1, and can be an existing electric cylinder, air cylinder or hydraulic cylinder.

[0030] The mechanical arm 102 is provided with a second horizontal chute, and the moving block 103 is slidably mounted on the second chute. The moving block 103 is connected to a second power mechanism so as to slide along the second chute under the drive of the second power mechanism. The second power mechanism is preferably arranged on the purification equipment body 1, and can be an existing electric cylinder, a cylinder or a hydraulic cylinder. The filler 101 is fixedly mounted on the moving block 103, and the filler 101 is connected to the pump body through a hose, and the second chute is perpendicular to the first chute. By moving the mechanical arm 102 along the first chute and the moving block 103 along the second chute, the filler 101 can be driven to move in the two-dimensional direction of the horizontal plane, thereby facilitating the filling of organic solvents into test tubes 201 at different positions. The pump body can draw the organic solvent into the filler 101. The moving block 103 is preferably exposed from the robot arm 102 , the filler 101 is installed inside the moving block 103 and the lower end of the filler 101 is exposed from the moving block 103 , so as to facilitate the installation and use of the moving block 103 and the filler 101 .

[0031] The fixing seat 2 is installed on the purification equipment body 1 and a receiving groove 3 is arranged at the top of the fixing seat 2 . The receiving groove 3 makes the top of the fixing seat 2 open, and the test tube 201 can be received in the receiving groove 3 .

[0032] The test tube rack 30 includes a placement plate 301 and a limiting plate 302 fixedly connected together, the placement plate 301 and the limiting plate 302 are both horizontal, and the placement plate 301 is located above the limiting plate 302, the placement plate 301 is located outside the receiving groove 3 and is supported by the top of the fixing seat 2, so that the test tube rack 30 can be removed from the fixing seat 2 as a whole after being placed on the fixing seat 2; the limiting plate 302 is located in the receiving groove 3, and the groove wall of the receiving groove 3 contacts the limiting plate 302 to limit the horizontal movement of the limiting plate 302, so that the test tube rack 30 can only move up and down but not horizontally after being placed on the fixing seat 2; the placement plate 301 and the limiting plate 302 can be two plates welded together, or they can be integrally formed. The top of the placement plate 301 is preferably provided with a plurality of handles 304 to facilitate the overall placement of the test tube rack 30.

[0033] The test tube rack 30 is provided with a plurality of through holes 303 for inserting the test tube 201, and a rubber sleeve 4 is fixed to the hole wall of each through hole 303 to prevent the inserted test tube 201 from falling. The through holes 303 on the test tube rack 30 are preferably arranged in an array, with the through holes 303 in each row parallel to the first slide groove, and the through holes 303 in the first column parallel to the second slide groove, so as to facilitate the injector 101 to inject the organic solvent into the test tube 201. The rubber sleeve 4 is preferably in the shape of a hollow cylinder, and its entire inner wall is in contact with the test tube 201, so as to have a good clamping effect on the test tube 201.

[0034] Furthermore, the accommodating groove 3 and the limiting plate 302 are both in the shape of a rectangular parallelepiped, and each side surface of the limiting plate 302 is respectively in contact with the groove wall of the accommodating groove 3, so that the groove wall of the accommodating groove 3 can well limit the limiting plate 302 to prevent the limiting plate 302 from horizontal movement and rotation, so that the limiting plate 302 cannot move at all in the horizontal direction.

[0035] Furthermore, there are multiple fillers 101 on the moving block 103 so as to simultaneously fill multiple test tubes 201 with organic solvent, thereby greatly improving the working efficiency of the protein purification device.

[0036] Furthermore, a refrigeration device is provided on the fixing seat 2 to cool the test tube 201 and the sample in the test tube in the receiving tank 3. The refrigeration device can control the temperature of the sample in the receiving tank 3, so that the protein purification device does not need to be carried out on an ice surface or in a cold storage when in use, which reduces the environmental requirements for protein purification and is convenient for staff to use. After the placement plate 301 is placed on the fixing seat 2, it completely covers the receiving tank 3 to prevent cold air leakage and save energy.

[0037] It is easy for those skilled in the art to understand that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A protein purification device, comprising a purification device body, a mechanical arm, a moving block, a filler, a fixing seat and a test tube rack, characterized in that: The purification equipment body is provided with a horizontal first slide groove, the mechanical arm is slidably mounted on the first slide groove, and the mechanical arm is connected to a first power mechanism to slide along the first slide groove under the drive of the first power mechanism; The mechanical arm is provided with a horizontal second slide groove, the moving block is slidably mounted on the second slide groove, the moving block is connected to a second power mechanism so as to slide along the second slide groove under the drive of the second power mechanism, the filler is fixedly mounted on the moving block, the filler is connected to the pump body through a hose, and the second slide groove is perpendicular to the first slide groove; The fixing seat is installed on the main body of the purification equipment and a receiving groove is arranged on the top of the fixing seat; The test tube rack comprises a placing plate and a limiting plate fixedly connected together, the placing plate and the limiting plate are both horizontal, and the placing plate is located above the limiting plate, the placing plate is located outside the receiving groove and is supported by the top end of the fixing seat, the limiting plate is located in the receiving groove, and the groove wall of the receiving groove contacts the limiting plate to limit the horizontal movement of the limiting plate; The test tube rack is provided with a plurality of through holes for inserting test tubes, and a rubber sleeve is fixed on the hole wall of each through hole to prevent the inserted test tube from falling.

2. A protein purification device according to claim 1, characterized in that: The containing groove and the limiting plate are both in a rectangular parallelepiped shape, and each side surface of the limiting plate is in contact with a groove wall of the containing groove.

3. A protein purification device according to claim 1, characterized in that: A plurality of handles are arranged on the top of the placement plate.

4. A protein purification device according to claim 1, characterized in that: The placement plate and the limiting plate are integrally formed.

5. A protein purification device according to claim 1, characterized in that: The through holes on the test tube rack are arranged in an array.

6. A protein purification device according to claim 1, characterized in that: The rubber sleeve is in the shape of a hollow cylinder.

7. A protein purification device according to claim 1, characterized in that: There are multiple injectors on the moving block so as to inject organic solvent into multiple test tubes at the same time.

8. A protein purification device according to claim 1, characterized in that: The fixing seat is provided with a refrigeration device for cooling the test tube in the containing tank.

9. A protein purification device according to claim 8, characterized in that: The placement plate completely covers the accommodating groove.

10. A protein purification device according to claim 1, characterized in that: The moving block is exposed from the mechanical arm, the filler is installed inside the moving block and the lower end of the filler is exposed from the moving block.

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