Anion exchange resin column structure for synthesizing cytidine monophosphate

Abstract

The utility model discloses an anion exchange resin column structure for synthesizing cytidine monophosphate, which comprises an exchange column body, a liquid inlet pipe and a regenerated liquid discharge pipe are arranged at the top of the exchange column body, and a liquid discharge pipe and a regenerated liquid filling pipe are arranged at the bottom of the exchange column body; the middle part of the exchange column body is divided into a plurality of compartments by filter plates, a static pressure chamber is reserved at the top, a confluence chamber is reserved at the top, and the compartments are filled with weakly alkaline organic acid anion exchange resin. Aiming at the impurity characteristics in the synthesis process of cytidine monophosphate, the utility model adopts a mode of feeding regeneration liquid from the bottom and discharging regeneration liquid from the top and countercurrent regeneration, and can ensure that the resin at the bottommost layer is in contact with the most fresh eluent, so that a good adsorption effect is kept in the next exchange, and the recovery rate is ensured. The resin is respectively arranged in the compartments in a manner of additionally arranging the partition plates, and although the resin can move in the compartments due to a suspended state in the exchange process, the partition plates can be maintained to be roughly layered and are not changed, so that the effect of countercurrent regeneration is ensured.

Description

Technical Field

[0001] The utility model relates to an ion exchange resin column, in particular to an anion exchange resin column structure used for cytidylic acid synthesis. Background Art

[0002] The molecular structure of cytidine derivatives usually contains a phosphate group. In a certain buffer system, the phosphate group often exists in a dissociated state, that is, it will carry a certain amount of negative charge. In addition, the number of phosphate groups of cytidine acid is generally 1-3 (such as cmp, cdp, ctp, etc.), and its derivatives with certain activities also have phosphate groups. Therefore, there are differences in the strength of the charge, but the type of charge is the same, that is, they all carry negatively charged groups, so cytidine acid can be purified by ion exchange.

[0003] Normal ion exchange resins remove impurities and leave the product, but there are many cytidylic acid derivatives, as well as catalysts, which are difficult to remove one by one, so reverse exchange is the only option, that is, the anions of cytidylic acid are adsorbed by the resin and then separated by elution with the regeneration liquid.

[0004] Currently, the eluent is eluted in a top-in, bottom-out manner, i.e., downstream elution. Since the resin in the upper layer has the most adsorption, the eluent is consumed more in the upper layer and carries a higher concentration of eluate, resulting in poor elution effect in the lower layer. When the eluent flows downstream, the product adheres to the resin in the lower layer. This part of the eluate will be washed away during the next exchange, which leads to poor regeneration effect. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide an anion exchange resin column structure for cytidylic acid synthesis, so as to solve the technical problems in the background technology.

[0006] In order to solve the above technical problems, the technical solution of the utility model is:

[0007] An anion exchange resin column structure for cytidylic acid synthesis comprises an exchange column, wherein the top of the exchange column is provided with a liquid inlet pipe and a regeneration liquid discharge pipe, and the bottom is provided with a liquid discharge pipe and a regeneration liquid filling pipe; the middle of the exchange column is divided into a plurality of compartments by a filter plate, a static pressure chamber is reserved at the top, and a confluence chamber is reserved at the top, and weakly alkaline organic acid anion exchange resin is filled in each compartment.

[0008] Furthermore, the regeneration liquid filling pipe is connected to the liquid inlet pipe, and the regeneration liquid discharge pipe is connected to the bottom side of the static pressure chamber and is arranged close to the topmost filter plate.

[0009] Furthermore, a connecting pipe is installed at the bottom position of each compartment corresponding to the side wall of the exchange column, and an electromagnetic isolation valve A is installed on the connecting pipe. The connecting pipe is connected to the resin delivery pipe in turn, and the resin delivery pipe is installed with an electromagnetic isolation valve B on the upper and lower sides of each connecting pipe connection position.

[0010] Furthermore, the liquid inlet pipe, the regeneration liquid discharge pipe, the liquid discharge pipe and the regeneration liquid filling pipe are all equipped with electromagnetic throttle valves for control.

[0011] The advantages of the utility model are:

[0012] The utility model adopts the method of regeneration liquid entering from the bottom and exiting from the top in view of the impurity characteristics in the cytidylic acid synthesis process, and countercurrent regeneration can ensure that the resin at the bottom layer contacts the freshest eluent, thereby maintaining a good adsorption effect in the next exchange and ensuring the recovery rate. By adding partitions, the resins are placed in the compartments respectively. Although the resins will move in the compartments due to the suspended state during the exchange process, the partitions can maintain the approximate stratification and will not change, thereby ensuring the effect of countercurrent regeneration. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is the front view of the utility model;

[0014] Figure 2 It is a cross-sectional view of the utility model.

[0015] In the figure, 1-exchange column, 11-partition, 12-static pressure chamber, 13-convergence chamber, 14-liquid inlet pipe, 15-liquid discharge pipe, 2-weakly basic organic acid anion exchange resin, 3-regeneration liquid filling pipe, 31-regeneration liquid discharge pipe, 4-connecting pipe, 41-electromagnetic isolation valve A, 5-resin delivery pipe, 51-electromagnetic isolation valve B, 6-electromagnetic throttle valve. DETAILED DESCRIPTION

[0016] The specific implementation methods of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these implementation methods is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in each implementation method of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0017] As shown in the figure:

[0018] An anion exchange resin column structure for cytidylic acid synthesis includes an exchange column 1, wherein the top of the exchange column 1 is provided with a liquid inlet pipe 14 and a regeneration liquid discharge pipe 31, and the bottom is provided with a liquid discharge pipe 15 and a regeneration liquid filling pipe 3; the middle of the exchange column 1 is divided into a plurality of compartments by a filter plate, a static pressure chamber 12 is reserved at the top, and a confluence chamber 13 is reserved at the top, and weakly alkaline organic acid anion exchange resin 2 is filled in each compartment.

[0019] The utility model makes some improvements to the traditional ion exchange resin column in view of the impurity characteristics in the synthesis process of cytidylic acid. First, a regeneration liquid inlet-uplet method is adopted. A regeneration liquid filling pipe 3 is arranged at the bottom, and a regeneration liquid discharge pipe 31 is arranged at the top. The resin at the top has a higher exchange rate, so most of the anions of cytidylic acid are adsorbed on the resin in the upper layer. Countercurrent regeneration can ensure that the resin at the bottom layer contacts the freshest eluent, thereby maintaining a good adsorption effect in the next exchange and ensuring the recovery rate.

[0020] To make it easier to understand, the exchange resin column is like a filtering cotton column. During exchange, the adsorption capacity at the top is large, while the adsorption capacity at the bottom is very small. If cleaning water is injected from the top to clean the cotton column, the pollution will be carried over to the bottom as well. However, if the bottom injection method is used, the bottom will not be polluted, but will only make the bottom cleaner without affecting the cleaning of the top.

[0021] Since the main anion in the synthesis process of cytidine acid is phosphate, and the acidity of phosphate is greater than that of cytidine acid, the resin uses weakly basic organic acid anion exchange resin 2, so that the anions of cytidine acid can be adsorbed specifically. The comb-shaped tertiary amine type weakly basic anion exchange resin (MKF-D30X) can be selected. The flexible comb-shaped long-chain functional groups of this resin have star-shaped diffusion and large degrees of freedom, and the adsorption capacity of organic weak acids is high and the desorption rate is also better.

[0022] However, the above premise is that the stratification of the resin in the exchange column 1 will not change, so the resins are placed in compartments separately by adding partitions 11. Although the resins will move in the compartments due to their suspended state during the exchange process, the partitions 11 can maintain the approximate stratification and will not change, thereby ensuring the effect of countercurrent regeneration.

[0023] As an optimized solution, the regeneration liquid filling pipe 3 is connected to the liquid inlet pipe 14, and the regeneration liquid discharge pipe 31 is connected to the bottom side of the static pressure chamber 12, and is arranged close to the topmost filter plate. In this way, the regeneration liquid will be discharged immediately after passing through the topmost resin, and there is no need to fill the static pressure chamber 12, which can effectively reduce the top pressure.

[0024] Due to the addition of the partition 11, the resin cannot be added at one time. A connecting pipe 4 can be installed on the side wall of the exchange column 1 at the bottom of each compartment, and an electromagnetic isolation valve A41 is installed on the connecting pipe 4. The connecting pipe 4 is connected to the resin delivery pipe 5 in turn, and the resin delivery pipe 5 is installed with an electromagnetic isolation valve B51 on the upper and lower sides of each connecting position of the connecting pipe 4.

[0025] When filling a certain compartment with resin, open the electromagnetic isolation valve A41 of the corresponding connecting pipe 4 and all the electromagnetic isolation valves B51 on the resin delivery pipe 5 located on the upper side of the connecting pipe 4, and use the water flow to carry the resin for filling. When discharging, open the electromagnetic isolation valve A41 of the corresponding connecting pipe 4 and all the electromagnetic isolation valves B51 on the resin delivery pipe 5 located on the lower side of the connecting pipe 4.

[0026] For the convenience of control, the liquid inlet pipe 14, the regeneration liquid discharge pipe 31, the liquid discharge pipe 15 and the regeneration liquid filling pipe 3 are all equipped with electromagnetic throttle valves 6 for control.

[0027] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the scope of protection of the present invention.

Claims

1. An anion exchange resin column structure for cytidylic acid synthesis, characterized in that: It includes an exchange column, wherein a liquid inlet pipe and a regeneration liquid discharge pipe are arranged at the top of the exchange column, and a liquid discharge pipe and a regeneration liquid filling pipe are arranged at the bottom; The middle part of the exchange column is divided into a plurality of compartments by a filter plate, a static pressure chamber is reserved at the top, and a confluence chamber is reserved at the top, and weakly alkaline organic acid anion exchange resin is filled in each compartment.

2. The anion exchange resin column structure for cytidylic acid synthesis according to claim 1, characterized in that: The regeneration liquid filling pipe is connected to the liquid inlet pipe, and the regeneration liquid discharge pipe is connected to the bottom side of the static pressure chamber and is arranged close to the topmost filter plate.

3. The anion exchange resin column structure for cytidylic acid synthesis according to claim 1, characterized in that: A connecting pipe is installed at the bottom position of each compartment corresponding to the side wall of the exchange column, and an electromagnetic isolation valve A is installed on the connecting pipe. The connecting pipe is connected to the resin delivery pipe in turn, and the resin delivery pipe is installed with an electromagnetic isolation valve B on the upper and lower sides of each connecting pipe connection position.

4. The anion exchange resin column structure for cytidylic acid synthesis according to claim 3, characterized in that: The liquid inlet pipe, the regeneration liquid discharge pipe, the liquid discharge pipe and the regeneration liquid filling pipe are all equipped with electromagnetic throttle valves for control.

Images