Effects of a decaffeinated green coffee extract on body weight control by regulation of glucose metabolism

Abstract

A method of controlling body weight in humans by administering an amount of decaffeinated green coffee extract effective to treat a subject. A preferred green coffee extract contains a ratio of 4-caffeoylquinic acid (4-CQA) to total chlorogenic acids (tCGA) (5-CQA / tCGA) of from about 0.1 to about 0.2. More preferably, the green coffee extract comprises from about 6% to about 8% of 4-caffeoylquinic acid and has a total chlorogenic acid concentration that exceeds about 45%. A preferred method of administration consists of administering the green coffee extracts is a dosage of about 200 mg twice a day prior to meals on an empty stomach.

Description

[0001] This application is a continuation-in-part and claims the benefit of a non-provisional U.S. patent application Ser. No. 12 / 263,292 filed on Oct. 31, 2008.FIELD OF THE INVENTION

[0002] The present invention relates to dietary supplements comprising a green coffee extract and methods relating to the administration of green coffee extract.BACKGROUND OF THE INVENTION

[0003] The prevalence of obesity (BMI≧30 kg / m2) continues to be a health concern for adults, children and adolescents in the United States. Data from the NHANES survey shows that among adult men the prevalence of obesity increased from 31.1% in 2003-04 to 32.2% in 2007-08, a small but not statistically significant change. Among adult women, the prevalence of obesity increased from 33.2% in 2003-04 to 35.5% in 2007-08, again a small but not significant change (Ogden et al. Gastroenterology 2007; 132 (6):2087-2102; Flegal et al. JAMA 2009; 303 (3):235-241). In Europe, the prevalence of obesity has increased by 10 to 38%, de...

Examples

example 1

In some embodiments, the green coffee extract comprises, Nutritional profiles of a green coffee extract (Svetol® green coffee extract, NATUREX). Nutritional analyses were conducted according to the European pharmacopoeia. Table 4 shows the nutritional value of the green coffee extract used in the examples reported herein (Svetol® green coffee extract, NATUREX).

[0049]

TABLE 4Nutritional profile of the green coffee extract (Svetol ® greencoffee extract, NATUREX).CompoundConcentrationLoss of drying [JO Mar. 11, 1977] 5%Proteins (Nx6.25) [JO Mar. 11, 1977] 9%Lipids [JO Mar. 11, 1977] 1%Ash [JO Mar. 11, 1977]10%Soluble sugars [JO Mar. 11, 1977]24%Caffeine [HPLC] 1%Total polyphenols [Folin-Ciocalteau]50%(tCGA) Total chlorogenic acids [HPLC]45%(5 CQA) 5-cajfeoylquinic acid [HPLC]>10% Cafestol [HPLC]Kahweol [HPLC]TOTAL100% 

[0050]The ratio between 5-caffeoylquinic acid (5-CQA) and total chlorogenic acids (tCGA) (5-CQA / tCGA in Table 4) of the green coffee extract (Svetol® green coffee extract,...

example 2

HPLC Chromatogram of the Green Coffee Extract (Svetol® Green Coffee Extract, NATUREX)

[0051]The high-pressure liquid chromatography chemical fingerprint for a green coffee extract (Svetol® green coffee extract, NATUREX) is presented in FIG. 6. The method for performing this analysis was as follows: HPLC-DAD was achieved using a column RP-C18 (5 μm-250×4.6 mm) at 55° C. The flow rate was 0.8 mL / min, and the elution was monitored at 330 nm. The mobile phases were (A) distilled water+H3PO4 0.002M, and (B) acetonitrile. A solution of 100% A was maintained during 8 min, increased by linear gradient to 35% A and 65% B by volume after 35 min total time; followed by a linear gradient of 100% A after maintaining this composition for 10 min; the system was then re-equilibrated to the initial composition. The content of CGA from 10 batches of green coffee extract (Svetol® green coffee extract, NATUREX) reported as mean±standard deviation (SD) is shown in Table 5.

TABLE 5Chlorogenic acids (CGA) o...

example 3

Composition of Total Chlorogenic Acids (tCGA) and 5-Caffeoylquinic Acid (5-CQA) in Several Coffee Extracts

[0052]Table 6 shows the composition of chlorogenic acids (tCGA) and 5-caffeoylquinic acid (5-CQA), as well as the ratio 5-CQA / tCGA, of several commercial extracts. All samples were analyzed using the HPLC method described in Example 2. The last two rows include the values of the green coffee extract described in this invention (Svetol® green coffee extract, NATUREX).

TABLE 6Composition of total chlorogenic acids (tCGA) and 5-caffeoylquinicacid (5-CQA) in several coffee extracts.tCGARatio 5-CQA / Coffee extract(HPLC)5-CQAtCGASpecieSvetol ®, Naturex146.4112.550.27C. robustaCommercial sample 15.572.20.39C. arabicaCommercial sample 26.072.20.36C. arabicaCommercial sample 35.551.980.36C. arabicaCommercial sample 450240.48C. arabicaCommercial sample 535180.51C. arabicaCommercial sample 645190.42C. arabicaCommercial sample 72750.19C. arabicaCommercial sample 853 / C. arabica(Batch 1)Commerc...

[0001] This application is a continuation-in-part and claims the benefit of a non-provisional U.S. patent application Ser. No. 12 / 263,292 filed on Oct. 31, 2008.FIELD OF THE INVENTION

[0002] The present invention relates to dietary supplements comprising a green coffee extract and methods relating to the administration of green coffee extract.BACKGROUND OF THE INVENTION

[0003] The prevalence of obesity (BMI≧30 kg / m2) continues to be a health concern for adults, children and adolescents in the United States. Data from the NHANES survey shows that among adult men the prevalence of obesity increased from 31.1% in 2003-04 to 32.2% in 2007-08, a small but not statistically significant change. Among adult women, the prevalence of obesity increased from 33.2% in 2003-04 to 35.5% in 2007-08, again a small but not significant change (Ogden et al. Gastroenterology 2007; 132 (6):2087-2102; Flegal et al. JAMA 2009; 303 (3):235-241). In Europe, the prevalence of obesity has increased by 10 to 38%, de...

Examples

example 1

In some embodiments, the green coffee extract comprises, Nutritional profiles of a green coffee extract (Svetol® green coffee extract, NATUREX). Nutritional analyses were conducted according to the European pharmacopoeia. Table 4 shows the nutritional value of the green coffee extract used in the examples reported herein (Svetol® green coffee extract, NATUREX).

[0049]

TABLE 4Nutritional profile of the green coffee extract (Svetol ® greencoffee extract, NATUREX).CompoundConcentrationLoss of drying [JO Mar. 11, 1977] 5%Proteins (Nx6.25) [JO Mar. 11, 1977] 9%Lipids [JO Mar. 11, 1977] 1%Ash [JO Mar. 11, 1977]10%Soluble sugars [JO Mar. 11, 1977]24%Caffeine [HPLC] 1%Total polyphenols [Folin-Ciocalteau]50%(tCGA) Total chlorogenic acids [HPLC]45%(5 CQA) 5-cajfeoylquinic acid [HPLC]>10% Cafestol [HPLC]Kahweol [HPLC]TOTAL100% 

[0050]The ratio between 5-caffeoylquinic acid (5-CQA) and total chlorogenic acids (tCGA) (5-CQA / tCGA in Table 4) of the green coffee extract (Svetol® green coffee extract,...

example 2

HPLC Chromatogram of the Green Coffee Extract (Svetol® Green Coffee Extract, NATUREX)

[0051]The high-pressure liquid chromatography chemical fingerprint for a green coffee extract (Svetol® green coffee extract, NATUREX) is presented in FIG. 6. The method for performing this analysis was as follows: HPLC-DAD was achieved using a column RP-C18 (5 μm-250×4.6 mm) at 55° C. The flow rate was 0.8 mL / min, and the elution was monitored at 330 nm. The mobile phases were (A) distilled water+H3PO4 0.002M, and (B) acetonitrile. A solution of 100% A was maintained during 8 min, increased by linear gradient to 35% A and 65% B by volume after 35 min total time; followed by a linear gradient of 100% A after maintaining this composition for 10 min; the system was then re-equilibrated to the initial composition. The content of CGA from 10 batches of green coffee extract (Svetol® green coffee extract, NATUREX) reported as mean±standard deviation (SD) is shown in Table 5.

TABLE 5Chlorogenic acids (CGA) o...

example 3

Composition of Total Chlorogenic Acids (tCGA) and 5-Caffeoylquinic Acid (5-CQA) in Several Coffee Extracts

[0052]Table 6 shows the composition of chlorogenic acids (tCGA) and 5-caffeoylquinic acid (5-CQA), as well as the ratio 5-CQA / tCGA, of several commercial extracts. All samples were analyzed using the HPLC method described in Example 2. The last two rows include the values of the green coffee extract described in this invention (Svetol® green coffee extract, NATUREX).

TABLE 6Composition of total chlorogenic acids (tCGA) and 5-caffeoylquinicacid (5-CQA) in several coffee extracts.tCGARatio 5-CQA / Coffee extract(HPLC)5-CQAtCGASpecieSvetol ®, Naturex146.4112.550.27C. robustaCommercial sample 15.572.20.39C. arabicaCommercial sample 26.072.20.36C. arabicaCommercial sample 35.551.980.36C. arabicaCommercial sample 450240.48C. arabicaCommercial sample 535180.51C. arabicaCommercial sample 645190.42C. arabicaCommercial sample 72750.19C. arabicaCommercial sample 853 / C. arabica(Batch 1)Commerc...

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