The invention discloses a region-selective synthesis method of 2,5-disubstituted pyrrole and belongs to the field of chemical synthesis. According to the method, simple pyrrole is used as a starting raw material, step-by-step iodination and coupling reaction are utilized, and six-step reaction is carried out, so that a series of 2,5-disubstituted pyrrole connected with different substituent groups, yields are more than or equal to 60% respectively, and structures are confirmed by virtue of 1H NMR, 13C NMR and HRMS. Compared with the traditional Paal-Knorr synthesis method, the region-selective synthesis method disclosed by the invention has the advantages that a building block molecule does not need to be constructed in advance, cheap and easy-to-get pyrrole is used as a raw material, a synthesis process is simplified, different substituent groups can be flexibly introduced to sites 2,5 of pyrrole, and a foundation is laid for synthesizing compounds such as drugs, molecular wires and macromolecular polymers.