The invention relates to the technical field of
stem cell extraction, in particular to a
plant stem cell extraction method. Comprising the following steps: SS01,
grinding, taking freeze-dried
plant tissues, carrying out fine
grinding and wall breaking to obtain tissue
fine powder, S02, extracting, putting the tissue
fine powder obtained in the step SS01 into a subcritical extractor, extracting to obtain an extraction
mixed solution, SS03, purifying, separating and purifying the extraction
mixed solution obtained in the step SS02 to obtain a tissue
stock solution, SS04, preparing a
stock solution, sS03, carrying out
centrifugal separation on the tissue
stock solution obtained in the step SS03, taking a lower turbid solution to obtain a
stem cell stock solution, SS05, extracting, and carrying out sampling extraction on the stem
cell stock solution obtained in the step SS04 by using
plant stem
cell extraction equipment in different periods, in different regions and in different times. The stem
cell extraction equipment disclosed by the invention has the beneficial effects that during sampling, through the arrangement of structures such as the back-cleaning
pipe, the back-cleaning flow channel and the back-cleaning hole, the stem
cell extraction equipment can realize self-cleaning of the extraction equipment when the stem
cell extraction equipment is used for sampling in different regions and times or in different times.