The invention discloses a high-nitrogen multi-effect biological enzyme organic fertilizer for garden flower planting, wherein the raw materials comprise chicken manure, cow dung, duck manure, domestic sewage, earthworm powder, rice straw, corn straw, soybean straw, hullessbarley straw, , maifanite, oil shale, coal gangue, phosphorite powder, peat soil, potassium ore powder, weathered coal, humic acid, echinochloa crusgalli (l.) beauv, artemisia sacrorum ledeb, sophora flavescens ait, pine needles, radix isatidis, nikkomycin powder, oceanospirillum powder, and enzyme microorganism. The invention further provides a preparation method of the high-nitrogen multi-effect biological enzyme organic fertilizer. According to the present invention, the high-nitrogen multi-effect biological enzyme organic fertilizer provides rich organic matters, inorganic matters, minerals and trace elements for plant growth, and has advantages of effective plant growth promoting, soil environment optimizing, plant yield improving, and plant quality improving.