Molecule marker of brassica napus self-incompatible maintainer as well as preparation and uses thereof
A Brassica napus and molecular marker technology, which is applied in the directions of biochemical equipment and methods, DNA preparation, and microbial determination/inspection, etc., can solve the problem of molecular markers and preparation methods of Brassica napus self-incompatibility maintainers that have not yet been seen. application and other issues to achieve the effect of reducing the workload of breeding, speeding up the pace, and shortening the breeding years
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[0038] 1. Production of Brassica napus self-incompatible hybrid F 1
[0039] Brassica napus self-incompatibility line S-1300 (see literature: Ma Chaozhi et al., Breeding of Brassica napus double-low self-incompatibility line, Journal of Huazhong Agricultural University, 1998, 17(3): 211-213 ) as the female parent, and Brassica napus self-incompatibility maintainer line 04P63 wai-52 (this line was preserved in the Chinese Type Culture Collection Center (English abbreviation: CCTCC) of Wuhan University, Wuhan City, Hubei Province on September 20, 2007 ), the preservation number is: CCTCC NO: P200704, the classification of this variety is named: Brassica napus) is the male parent for crossing, and obtains F 1 seed.
[0040] 2. Screening self-incompatibility segregation populations of Brassica napus
[0041] F obtained by the above steps 1 Seeds were sown under natural conditions to obtain Brassica napus self-incompatibility F 1 plants. in F 1 (S-1300×04P63external-52)F 2 ...
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