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Universal method for rapid detection and structural identification for Chinese traditional medicine complex component

A general-purpose, traditional Chinese medicine technology, applied in the direction of material separation, analysis materials, instruments, etc., can solve the problems of increasing research difficulty, time-consuming, unfavorable generalization, etc., to achieve universal applicability, high-quality precision sensitivity, high-quality accuracy degree of effect

Inactive Publication Date: 2009-04-01
CHINA PHARM UNIV +1
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Problems solved by technology

However, it is found in the research that dozens or even hundreds of candidate structures of an unknown compound of a traditional Chinese medicine can be obtained through chemical formula search. It is very time-consuming to screen out the real structure, which greatly increases the difficulty of research and is not conducive to general promotion.

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  • Universal method for rapid detection and structural identification for Chinese traditional medicine complex component
  • Universal method for rapid detection and structural identification for Chinese traditional medicine complex component
  • Universal method for rapid detection and structural identification for Chinese traditional medicine complex component

Examples

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Embodiment 1

[0034] Example 1: Rapid detection and structural identification of unknown components of Mailuoning Injection

[0035] 1. Extraction of all ingredients Mailuoning Injection:

[0036] Previous studies have shown that the concentration of acidic components in Mailuoning Injection is high, which seriously interferes with the response of low-concentration non-acidic components during simultaneous detection. In view of this, the present invention adopts tandem solid phase extraction method to extract Mailuoning Injection, first uses MAX column to extract acidic components, and then uses HLB column to extract non-acidic components, in order to extract as many formulation components as possible.

[0037] After diluting Mailuoning injection (No.200611032, produced by Nanjing Jinling Pharmaceutical Co., Ltd.) 10 times with ultrapure water, the sample was loaded onto the activated MAX column and the HLB column successively, and then washed with 50% ammonia water, methanol and Wash with...

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Abstract

The invention belongs to the pharmaceutical field and relates to the fast detection and structural identification of complex components of the traditional Chinese medicine. The invention provides a commonality method for the fast detection and structural identification of the complex components of the traditional Chinese medicine, which comprises the following steps: 1. the extraction of all the components; 2. mass spectrometric analysis; 3. determining the main components; 4. multi-stage mass spectrum data acquisition; 5. determining diagnosis ions and the components are grouped according to the diagnosis; 6. connecting all the groups into a component network by bridging components; 7. carrying out database search by predicting chemical formulas and obtaining a candidate structure set of each component; 8. firstly identifying the component with the least candidate structure in the group with the most components, according to that, analyzing the structure of the group of diagnosis ions, then screening the candidate structure of other components in the group by taking the diagnosis ions as standards, carrying out identification, finally obtaining the structure of all the diagnosis ions by the bridging components and further identifying all the components in the component network; and 9. identifying the components which are not grouped and separated from the component network by the continuous screening of specific neutral loss.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to the rapid detection and structure identification of complex components of traditional Chinese medicines, in particular to a structure identification method for unknown components in Mailuoning injection. Background technique [0002] Traditional Chinese medicine is the traditional treasure of the Chinese nation, which has made great contributions to the health and reproduction of the Chinese nation. It is also the most advantageous and distinctive discipline in the field of natural science in the world. As we all know, the composition analysis of traditional Chinese medicine is a key step to reveal the material basis of its medicinal effect, which is of great significance to the quality control of traditional Chinese medicine preparations. However, the chemical composition of traditional Chinese medicine preparations is very complex, with the characteristics of multi-component, multi-target,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
Inventor 王广基崔楠郝海平
Owner CHINA PHARM UNIV
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