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Method for activation of helper t cell and composition for use in the method

A composition and cell technology, applied in chemical instruments and methods, biochemical equipment and methods, drug combinations, etc., can solve problems such as difficulty in identifying promiscuous auxiliary peptides

Inactive Publication Date: 2010-01-06
INT INST OF CANCER IMMUNOLOGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is very difficult to identify promiscuous helper peptides that bind and function adequately with three or more MHC class II molecules

Method used

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  • Method for activation of helper t cell and composition for use in the method
  • Method for activation of helper t cell and composition for use in the method
  • Method for activation of helper t cell and composition for use in the method

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Experimental program
Comparison scheme
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Embodiment Construction

[0053]

1. Preparation of antigen presenting cells

Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood collected from healthy blood donors (HLA-DRB1*1501 positive, HLA-DPB1*0901 positive or HLA-DPB1*0501 positive). In 1% AB serum (Nabi, Miami, FL), X-VIVO 15 medium (Cambrex) with 1×10 7 Density of cells / well PBMCs were seeded in a 6-well plastic plate and cultured for 2 hours. After culture, the suspended cells were removed, and the remaining adherent cells were cultured in 1000IU / ml IL-4 (PeproTech), 1000IU / ml GM-CSF (PeproTech), 1% AB serum and X-VIVO 15 medium. On the 2nd and 4th day, the medium was changed and IL-4 and GM-CSF were added. On day 6, 100IU / ml TNF-α was added to mature antigen presenting cells.

[0054]

2. Induction of WT1 peptide-specific CD4 positive T cells

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Abstract

Disclosed are: a method for activating a helper T cell, which comprises the step of adding a WT1 peptide to an antigen-presenting cell to activate the helper T cell, wherein the WT1 peptide is capable of binding to any one selected from an HLA-DRB1<*>1501 molecule, an HLA-DPB1<*>0901 molecule and an HLA-DPB1<*>0501 molecule; a composition for use in the method; a therapeutic and / or prophylactic method for cancer by activating a helper T cell; a pharmaceutical composition for use in the therapeutic and / or prophylactic method; and others.

Description

Technical field [0001] The present invention relates to a method for activating helper T cells, including adding a WT1 peptide to an antigen presenting cell, and thereby activating the helper T cell, wherein the WT1 peptide has binding HLA-DRB1*1501 molecules, HLA-DPB1*0901 The ability of any one of the molecule and the HLA-DPB1*0501 molecule, and the composition used therefor, the pharmaceutical composition for treating and / preventing cancer by activating helper T cells, etc. Background technique [0002] The WT1 gene (Wilms' tumor 1 gene) is considered to be a gene that causes Wilms' tumor (which is childhood kidney cancer) (Non-Patent Documents 1 and 2). WT1 is a transcription factor with a zinc finger structure. Initially, the WT1 gene was considered a tumor suppressor gene. However, subsequent studies (Non-Patent Documents 3, 4, 5, and 6) revealed that the WT1 gene functions as an oncogene in hematopoietic tumors and solid cancers. [0003] It has been shown that WT1 pep...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N15/00C07K14/82C07K16/32A61K38/00A61P35/00A61P35/02A61P43/00C12Q1/02G01N33/53A61K39/00C07K7/08C12N5/0783C12N5/10C12Q1/04
CPCA61K39/0011C07K14/4748G01N33/505A61K38/00G01N33/574A61K2039/55516G01N33/68A61P35/00A61P35/02A61P37/04A61P43/00A61K39/001153A61K39/464453A61K39/4611C12N5/0636C07K14/82C07K16/32C12N15/09
Inventor 杉山治夫
Owner INT INST OF CANCER IMMUNOLOGY INC
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