Beverage for supplementing human body energy and recovering people from fatigue
A technology for restoring fatigue and human energy, applied in the field of beverages to achieve the effects of no toxic side effects, maintaining acid-base balance, and improving immunity
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Embodiment 1
[0014] Embodiment 1: Preparation of glucose-6-phosphate nutrient solution
[0015] (1) Preparation of glucose-6-phosphate nutrient solution by traditional enzymatic method
[0016] Add 6g of ATP, 20g of glucose and 2g of magnesium chloride in a 1000ml flask, and add 2mg of phosphohexokinase under the conditions of pH 6.5 and 38°C, and keep the reaction for 4 hours. After the reaction, filter with a 6K ultrafiltration membrane and collect the filtrate. Then ATP and ADP are recovered with Cl type anion chromatography column. Then use Na-type cation chromatography column to remove part of Mg 2+ Afterwards, after concentration, a solution containing about 5% glucose-6-phosphate and 5% glucose was obtained. Finally, add appropriate amount of KCl, NaCl, vitamin C and concentrated fruit juice for seasoning to prepare glucose-6-phosphate nutrient solution.
[0017] (2) Preparation of glucose-6-phosphate nutrient solution by solid-phase enzymatic method
[0018] The process of pre...
Embodiment 2
[0019] Embodiment 2: the animal experiment of anti-fatigue of 6-glucose phosphate nutrient solution
[0020] (1) Experimental materials:
[0021] Experimental samples: 10% glucose hexaphosphate solution, 10% glucose solution.
[0022] Experimental animals: 21 Kunming white mice (male), weighing 202 g, and aged about 3 weeks.
[0023] Experimental equipment: swimming pool (45cm×35cm×35cm), timer
[0024] (2) Experimental method:
[0025] The mice were randomly divided into 3 groups: blank control group, glucose hexaphosphate group, and glucose group, with 7 mice in each group. Each group was given physiological saline, 10% glucose hexaphosphate solution, and 10% glucose solution by intragastric administration. Before administration, the mice in each group performed weight-bearing swimming (see below for the method) to make them physically exhausted, and then administered the drug by gavage immediately. After 30 minutes, they performed weight-bearing swimming again, and the ...
Embodiment 3
[0031] Embodiment 3: 6-glucose phosphate nutrient solution rapid recovery exercise fatigue test
[0032] (1) Test subjects: 20 postgraduates who volunteered to participate in the test participated in the test, the ratio of male to female was 10:10, and the age was 23 to 30 years old. The test time was the beginning of September 2010, and the temperature on that day was 34°C. The experimenter jogged 1000 meters in the stadium. The results showed that there were 14 people who had obvious thirst, bitter taste, irritability, dizziness and fatigue. There are 6 people with mild symptoms above.
[0033] (2) Test method: Select 14 people with obvious fatigue feeling to participate in the test. After jogging for 1000 meters, each person rested on the spot, and each person drank a bottle of 300ml beverage within 10 minutes. The participants in the experiment were divided into 3 groups, the first group consisted of 5 persons, who drank nutrient solution containing 5% glucose 6-phosph...
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