High resolution, high throughput hla genotyping by clonal sequencing
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- F HOFFMANN LA ROCHE & CO AG
- Publication Date
- 2011-07-13
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
Background of the invention
[0001] Class I and class II HLA loci are the most polymorphic genes in the human genome, and the complex pattern of patch polymorphism is mainly localized in exon 2 of class II genes and exons 2 and 3 of class I genes middle. Allele-level resolution of HLA alleles that are clinically important for hematopoietic stem cell transplantation is technically challenging with current HLA typing methods. Several large-scale studies have demonstrated precise, allele-level HLA differentiation between donor and patient by reducing the incidence and severity of acute and chronic graft-versus-host disease and improving the rate of successful engraftment Matching significantly improved overall graft survival. For example, when 8 of the 8 most prominent HLA loci were matched, compared with 6 of 8, post-transplant survival was 60% better after 12 months.
[0002] Current practice is to maintain a bone marrow donor registry in which millions of potential donors ar...