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Pinellia ternate tissue culture one-step speciation method

A tissue culture and Pinellia technology, applied in the field of biotechnology engineering, can solve the problems of low transplant survival rate, reduce the steps of seedling hardening, reduce the possibility of virus infection, and avoid the effect of transplant survival rate.

Inactive Publication Date: 2011-11-02
HANZHONG PLANTS RES INST
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Problems solved by technology

[0008] The purpose of the present invention is: aiming at the problem that the transplanting survival rate of Pinellia is relatively low in the one-step seedling process, we have further improved the method of tissue culture of Pinellia, and proposed the one-step seeding method of Pinellia tissue culture

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  • Pinellia ternate tissue culture one-step speciation method
  • Pinellia ternate tissue culture one-step speciation method

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Embodiment Construction

[0014] Below in conjunction with embodiment the present invention will be further described,

[0015] The technological process of the one-step seeding method of pinellia tissue culture:

[0016] combine figure 1 , in this process, the method of subculture value-added culture of pinellia tissue culture is improved, and the leaf petiole and tuber of mature seedlings are recultivated. The reason is that in the traditional tissue culture subculture proliferation, multiple transfers cause material Variation, so that it can not normally form a complete plant. Through this improvement, the plant differentiation rate, the number of differentiated tubers on each explant, and the time of seedling formation are all superior to the former, and at the same time, no variation occurs, and all the genetic characteristics of Pinellia can be completely retained.

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Abstract

The invention relates to a pinellia ternate tissue culture one-step speciation method. Pinellia ternate tissue culture seedlings, that are obtained from a one-step seedling and can be treated with hardening and transplanting, are injected with liquid medium without switch, and cultured sequentially at 25+- 2 DEG C, with a photoperiodicity of 12h / d and an illumination intensity of 1000-1500Lx. Pinellia ternates complete one growth period in a culture bottle, and are washed out from the medium to be transplanted. The invention is characterized in that the method simplifies hardening seedling steps, avoids problem of transplanting survival rate, and lowers the possibility of re-infection of virus in pinellia ternate first generation production. In addition, the method combines all merits of the one-step speciation method, so that pinellia ternate plants have good inductivity, differentiation rate, and number of tubers differentiated from each explant.

Description

technical field [0001] The invention relates to a one-step seeding method of pinellia tissue culture, which belongs to the field of biotechnology engineering. Background technique [0002] Pinellia ternata (Thunb.) Breit. is a perennial herbaceous plant of Araceae. Clinical application is very extensive, and it has a medicinal history of more than 2,000 years. It is a commonly used traditional Chinese medicine that is widely used blindly and has relatively definite curative effects. [0003] Pinellia wild resources are widely distributed, but due to changes in farming systems and people’s over-excavation, the ecological environment suitable for the growth of Pinellia is shrinking year by year. The range of applications is expanding day by day, and the market supply has been tight. In order to alleviate the contradiction between supply and demand of pinellia, my country has started to conduct experimental research on wild species of pinellia since the early 1980s. However, ...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘万里何忠军
Owner HANZHONG PLANTS RES INST
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